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All Journal Jurnal Ilmiah Farmasi Pharmaciana: Jurnal Kefarmasian Majalah Obat Tradisional Universa Medicina Acta Pharmaciae Indonesia : Acta Pharm Indo ALCHEMY Jurnal Penelitian Kimia JPSCR : Journal of Pharmaceutical Science and Clinical Research International Journal of Basic and Applied Science Journal of Science and Technology Research for Pharmacy Pharmaceutical Sciences and Research (PSR) Pharmaqueous Molekul: Jurnal Ilmiah Kimia Jurnal Pengabdian Gizi dan Kesehatan Masyarakat
Triyadi Hendra Wijaya
Department Of Pharmacy, Faculty Of Health Sciences, Universitas Jenderal Soedirman, Jl. Dr. Soeparno, Purwokerto, Indonesia, 53122.
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Electrical & Electronics Engineering Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health

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Nephroprotective effect and untargeted metabolomic analysis of celery extract against diethylene glycol induced nephrotoxicity in rats Wijaya, Triyadi Hendra; Ekowati, Heny; Wasito, Hendri; Maryellen, Geeta; Silitonga, Tasya Melinda; Oktavian, Firdaus
Universa Medicina Vol. 44 No. 2 (2025)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2025.v44.190-200

Abstract

Background Diethylene glycol (DEG) is known for its nephrotoxic effects as a contaminant. Recent investigations suggest that celery extract potentially protects against DEG-induced renal toxicity, warranting an exploration of the non-targeted metabolomic profile of celery in renal tissue. This study aimed to determine celery nephroprotective effects and identify potential biomarkers using an untargeted metabolomic  celery profile in DEG-induced kidney injury in male rats. Methods An experimental study was conducted involving 24 male Wistar rats, randomized into control and three treatment groups receiving DEG at doses of 1, 3, and 5 g/BW for three days. After 14 days, all rats were sacrificed. For the celery nephroprotective and metabolomic study, 27 male rats were randomized into three groups:  control, DEG group at 3g/BW, and DEG-celery group (receiving celery extract 200mg/kg BW single dose for 6 days). After 14 days, blood was tested for creatinine level and untargeted metabolomic analysis using liquid chromatography-high resolution mass spectrometry. One-way ANOVA was used to analyze creatinine data. Results Diethylene glycol at doses of 1 and 3 g/kg BW caused mild renal damage, while 5 g/kg BW resulted in moderate damage. Creatinine levels in the normal, DEG-induced, and DEG-celery groups were 0.55 ± 0.14, 0.71±0.1, and 0.86± 0.17 mg/dL, respectively, but not statistically significantly different between groups (p>0.05). DEG lead tryptophan and phenylalanine levels increased 45-fold and 123-fold, respectively. Celery reduced levels of these metabolites 21-fold and 65-fold, respectively. Conclusion Diethylene glycol led to mild to moderate renal damage. Celery effectively diminished metabolite levels associated with renal damage, specifically tryptophan and phenylalanine.
Nephroprotective and Hepatoprotective Effects of Turmeric in Diethylene Glycol Induced Toxicity in Rats Ekowati, Heny; Devitasari, Arinda; Fitriani, Novita Inawanda; Endriastuti, Nialiana Endah; Wijaya, Triyadi Hendra
Majalah Obat Tradisional Vol 30, No 2 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.95225

Abstract

In 2022, children in Indonesia experienced acute renal failure due to antipyretic syrup contaminated with diethylene glycol (DEG). DEG is a known contaminant in pharmaceutical solvents that can induce inflammation and act as a free radical, leading to kidney and liver damage. Turmeric (Curcuma Longa) has been reported to have nephroprotective and hepatoprotective effects due to anti-inflammatory and antioxidant properties. This study aims to evaluate the protective effects of turmeric ethanol extract on rat kidneys and liver subjected to DEG toxicity. Rats were divided into five groups (n=5 per group): Group I served as the normal control, while Group II received DEG orally at 3 g/kg BW twice daily for three days. Groups III, IV, and V were treated with DEG (3 g/kg BW) along with turmeric ethanol extract at a doses of 100, 200, and 400 mg/kg BW, respectively, administered orally twice daily for six days. After 14 days, all rats were sacrificed for macroscopic and microscopic evaluation using hematoxylin-eosin (H&E) staining. The results showed that rats treated with turmeric extract exhibited significantly significantly less kidney and liver damage (p<0.05). Kidney protection was evidenced by improvements in endothelial tissue, glomeruli, and tubules, while liver protection was indicated by reduced Kupffer cell activation, sinusoidal dilatation, hepatocyte degeneration, and necrosis. In conclusion, turmeric ethanol extract effectively protects against DEG-induced kidney and liver toxicity in rats.
Chemometric analysis of fingerprinting derivative spectrophotometry for authentication of shallots Puteri, Adelia; Wijaya, Triyadi Hendra; Wasito, Hendri
International Journal of Basic and Applied Science Vol. 13 No. 2 (2024): Sep: Basic and Applied Science
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/ijobas.v13i2.406

Abstract

The Bima Brebes, variety of shallots, was in high demand, which led to mixing with other varieties. Derivative spectrophotometric fingerprinting combined with chemometrics was used to distinguish between authentic and adulterated shallot varieties. The objective of this study was to identify the original spectra and their derivative spectrophotometric fingerprinting, as well as classify and differentiate between shallot varieties using chemometrics. UV-Visible (UV-Vis) spectrophotometry was used to test essential oil samples from three shallot varieties and their mixtures, followed by spectral derivatization. The spectral data revealed distinct patterns for each sample, including individual varieties and mixtures, and was then analyzed using Principal Component Analysis (PCA) and Partial Least Square-Discriminant Analysis (PLS-DA). The original spectra and their derivatives showed similarities across the samples. PCA and PLS-DA results indicated that the second-order derivative data provided the greatest separation, with a total Principal Component 1 (PC1) and Principal Component 2 (PC2) value of 62.2%, a total component 1 and 2 value of 60.1%, and the highest Variable Importance in the Projection (VIP) score wavelength of 225 nm. The PLS-DA results were validated to ensure that the model was not overfit, as evidenced by a satisfactory cross-validation quality (Q2/R2) value of 0.693 and a significant permutation test. The combination of derivative spectrophotometry fingerprinting and a chemometric approach effectively classified different samples, allowing for the determination of the authenticity of a specific shallot variety.
Analisis Pengaruh Wadah, Suhu Dan Lama Penyimpanan Minyak Hati Ikan Cucut Botol Terhadap Bilangan Peroksida Wijaya, Triyadi Hendra; Issusilaningtyas, Elisa; Faiqoh, Maziyatul
Pharmaqueous: Jurnal Ilmiah Kefarmasian Vol. 1 No. 1 (2019): Volume 1, No. 1, November 2019
Publisher : Universitas Al-Irsyad Cilacap

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36760/jp.v1i1.14

Abstract

Stabilitas oksidatif digunakan untuk mengukur perubahan kualitas minyak seiring dengan cara, suhu dan lama waktu simpan. Stabilitas oksidatif dapat diukur menggunakan bilangan peroksida. Dalam penelitian ini telah diteliti mengenai pengaruh cara, suhu, lama waktu penyimpanan terhadap stabilitas oksidatif minyak hati ikan cucut botol. Bilangan peroksida diukur menggunakan titrasi iodimetri. Data dianalisis menggunakan statistik deskriptif dan Anova yang diikuti dengan uji Tukey untuk mengetahui perbedaan antar kelompok perlakuan. Kelompok dengan suhu 25 0C dengan wadah semi transparan dan transparan menunjukkan kenaikan bilangan peroksida yang cukup tajam dibandingkan dengan kelompok dengan wadah gelap dan pada kelompok dengan suhu -28 0C dan 5 0C. Wadah gelap dapat melindungi dari cahaya matahari (sinar uv) sehingga dapat meminimalisir terjadinya oksidasi. Suhu -28 0C dan 50C dapat melindungi minyak hati ikan cucut botol dari oksidasi dan tidak tergantung pada wadah yang digunakan.
Analisis Kandungan Boraks dan Formalin pada Bakso dan Cilok di Wilayah Cilacap Kota Faoziyah, Anita Ratna; Agustina, Laely Triyani; Wijaya, Triyadi Hendra
Pharmaqueous: Jurnal Ilmiah Kefarmasian Vol. 1 No. 1 (2019): Volume 1, No. 1, November 2019
Publisher : Universitas Al-Irsyad Cilacap

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36760/jp.v1i1.22

Abstract

Telah dilakukan penelitian kandungan borak dan formalin pada bakso dan cilok di Wilayah Cilacap Kota. Wilayah Cilacap Kota meliputi tiga kecamatan yaitu Kecamatan Cilacap Tengah, Kecamatan Cilacap Utara dan Kecamatan Cilacap Selatan. Jumlah sempel yang digunakan sebanyak 30 sempel dengan teknik pengembilan sempel mengunakan rendom sampling. Hasil identifikasi kandungan borak dengan metode analisis kualitatif menunjukan bahwa bakso dan cilok yang beredar di Wilayah Cilacap Kota tidak mengandung borak dengan presentase 100% negative (tidak mengandung borak), sedangan hasil identifikasi kandungan formalin pada bakso dan cilok di Wilayah Cilacap kota menunjukan sebanyak 73,33% bakso dan cilok mengandung formalin. Hasil identifikasi ini ditunjukan dengan adanya perubahan warna pada sempel bakso dan cilok setelah direaksikan dengan larutan KMnO4. Perubahan warna yang terjadi pada hasil identifikasi sempel yang mengandung formalin yaitu terjadi perubahan warna dari warna ungu menjadi warna kecoklatan.
Synthesis and Cytotoxic Activity of Methoxylated Chalcones in Breast Cancer MCF-7 and Prostate Cancer DU-145 Cell Lines Fareza, Muhamad Salman; Samudra, Genta Hafied Naga; Asrada, Syahdan; Fischellya, Dafi; Wijaya, Triyadi Hendra; Choironi, Nur Amalia; Wasito, Hendri; Suhesti, Tuti Sri; Mustikaningtyas, Ika; Rehana, Rehana; Setiyabudi, Lulu; Sarmoko, Sarmoko
Molekul Vol 20 No 3 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.3.13612

Abstract

Chalcones, a class of naturally occurring compounds, exhibit a broad spectrum of biological activities, including anticancer properties. In this study, a series of methoxylated chalcones were synthesized via Claisen-Schmidt condensation and evaluated for cytotoxic activity against breast cancer MCF-7 and prostate cancer DU-145 cell lines. The synthetic route involved Claisen-Schmidt condensation, leading to various methoxy-substituted chalcone derivatives. The structures of the synthesized chalcones were confirmed through NMR and mass spectrometry. Cytotoxicity was assessed using the PrestoBlue assay, with 4-bromochalcone (compound 2) displaying the highest cytotoxic activity against MCF-7 cancer cell lines (IC50 = 26.99 µM). These results indicate that methoxylated chalcones hold promise as potential lead compounds for the development of new anticancer agents targeting breast and prostate cancer.
Co-Authors Agnes Yuliasari Agustina, Laely Triyani Anita Ratna Faoziyah, Anita Ratna Anjani, Sabrina Asrada, Syahdan Beti Pudyastuti Beti Pudyastuti Beti Pudyastuti, Beti Choironi, Nur Amalia Devitasari, Arinda Dhadhang Wahyu Kurniawan Dhadhang Wahyu Kurniawan Elisa Issusilaningtyas Endriastuti, Nialiana Endah Faiqoh, Maziyatul Fareza, Muhamad Salman Fischellya, Dafi Fitriani, Novita Inawanda Giri Gumelar Hendri Wasito HENDRI WASITO Heny Ekowati Ilma Nugrahani Joko Setyono Kartawinata, Tutus Gusdinar Khoiriani, Izzati Nur Maryellen, Geeta Muhamad Salman Fareza Muhamad Salman Fareza Mustikaningtyas, Ika Nur Amalia Choironi Nur Intan, Nur Nuraeni Ekowati, Nuraeni Oktavian, Firdaus Prilyano Garcella Puteri, Adelia Rahma, Shesa Rahmah, Annisa Rehana, Rehana Samudra, Genta Hafied Naga Sarmoko Sarmoko Setiyabudi, Lulu Silitonga, Tasya Melinda Sri Sutji Susilowati Sri Sutji Susilowati Sunarto Sunarto Sunarto Sunarto Teguh Jati Prasetyo Tuti Sri Suhesti Warsinah Warsinah Wicaksari, Sifa Aulia Yosephine, Samuella Yuliasari, Agnes