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Journal : Althea Medical Journal

Association between Mothers’ Characteristics, Knowledge, Attitude, and Practice and Intestinal Helminthes Infection on Children Hakiki, Nadhira Permata; Faridah, Lia; Dhamayanti, Meita
Althea Medical Journal Vol 3, No 2 (2016)
Publisher : Althea Medical Journal

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Abstract

Background: The prevalence of soil-transmitted helminthes infection in Indonesia is still high, especially in children aged 3 to 8 years old. Helminthes infection cause loss of nutrition, delay physical development, intelligence, and labor productivity and decrease immunity. Mothers’ characteristics, knowledge, attitude, and practice are some of the factors that influence the occurrence of intestinal helminthes infection on children. This study was aimed to find the association between mothers’ characteristics, knowledge, attitude, and practice and the occurrence of intestinal helminthes infection on children.Methods: The study was conducted at Jatinangor Cohort’s research center from August to September 2014 using cross sectional analysis. One hundred and forty five secondary data were collected using validated questionnaire that filled by mothers and results of feces on children were tested. The amount of data excluded due to incomplete was 8, the data utilized was then analyzed by Chi Square evaluation.Results: Mothers’ characteristics such as age (P = 0.611), education (P = 0.952), occupation (P = 0.876), income (P = 0.199), and knowledge (P = 0.424; OR = 1.333), attitude (P = 0.236; OR = 0.808), practice (P = 0.333; OR = 4.625) did not have a significant association with the occurrence of intestinal helminthes infection on children.Conclusions: Characteristics, knowledge, attitude, and practice of the mothers towards the intestinal helminthes infection do not associate with the occurrence of intestinal helminthes infection on children. [AMJ.2016;3(2):248–53] DOI: 10.15850/amj.v3n2.794
Identification of Giardia lamblia, Entamoeba histolytica and Cryptosporidium sp. in Feces of Diarrheal Patient at Puskesmas Jatinangor, September–November 2012 Palani, Komathi; Faridah, Lia; Sumardi, H. Uun
Althea Medical Journal Vol 2, No 2 (2015)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (529.961 KB)

Abstract

Background: Diarrhea is one of the main public health problems occurring in West Java. One of the affected areas is Subdistrict Jatinangor. Inappropriate management of sanitation facilities around Jatinangor area causes contamination of water. Cikeruh River is one of the water sources in Jatinangor Area, from which people obtain water for daily activities. Water borne illness due to poor sanitation condition can lead to parasitic infection such as Giardia lamblia, Entamoeba histolytica and Cryptosporidium parvum whichcan cause a prolonged diarrhea. There has not been any study done regarding the presence of parasitical infection causing diarrhea around Jatinangor.Methods: In order to identify the parasitic infection, a descriptive study was carried out on 16 fecal samples collected from diarrheal patient who visited Puskesmas Jatinangor from September–November 2012. The parasites were checked by using wet mount methodResults: The parasites found were Entamoeba histolytica, Cryptosporidium parvum, but none of Giardia lamblia. There were also other findings such as Iodamoeba butschlii and Entamoeba coli.Conclusion: Positive findings of Entamoeba histolytica and Cryptosporidium parvum in diarrhea patients is most probably due to contaminated water and food. Measures need to be done to improve sanitary condition in Cikeruh River to prevent diarrhea. [AMJ.2015;2(1):213–16]
Association of Ascariasis with Nutritional and Anemic Status in Early School-Age Students Eidwina, Chin Annrie; Faridah, Lia; Ermaya, Yudith Setiati; Akhmad Gurnida, Dida
Althea Medical Journal Vol 3, No 1 (2016)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (527.852 KB)

Abstract

Background: Ascariasis is one of the most frequent helminthias is that occurred in school-age children. Commonly, severe intensity of infection will seriously affect the nutritional and anemic status of the students. The aim of this study was to determine the association of ascariasis with nutritional and anemic status in early school-age students. Methods: An analytical cross-sectional study was conducted based on the secondary data from Jatinangor Cohort. The secondary data of 74 students who met the criteria were included in this study. Data collection was conducted in the Department of Epidemiology and Biostatistics from August to September 2014. The data obtained was analyzed based on the characteristics of the students regarding gender, age, class, parents’ education, ascariasis, nutritional and anemic status. Then, the data were further analyzed to determine the association of ascariasis with nutritional and anemic status of the students using the chi square test or Fisher test if the requirement was not fulfilled.Results: Sixteen (22%) students were having Ascaris lumbricoides infection, six (8.1%) students were thin and seventeen (23%) students were anemic. There was no statistically significant association found of Ascaris lumbricoides infection with nutritional and anemic status in early school-age students (P value <0.05) in this study.Conclusions: There is no statistically significant association of Ascaris lumbricoides infection with nutritional and anemic status of the early school-age students. [AMJ.2016;3(1):93–8] DOI: 10.15850/amj.v3n1.710
Association of Ascariasis with Nutritional and Anemic Status in Early School-Age Students Chin Annrie Eidwina; Lia Faridah; Yudith Setiati Ermaya; Dida Akhmad Gurnida
Althea Medical Journal Vol 3, No 1 (2016)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (527.852 KB)

Abstract

Background: Ascariasis is one of the most frequent helminthias is that occurred in school-age children. Commonly, severe intensity of infection will seriously affect the nutritional and anemic status of the students. The aim of this study was to determine the association of ascariasis with nutritional and anemic status in early school-age students. Methods: An analytical cross-sectional study was conducted based on the secondary data from Jatinangor Cohort. The secondary data of 74 students who met the criteria were included in this study. Data collection was conducted in the Department of Epidemiology and Biostatistics from August to September 2014. The data obtained was analyzed based on the characteristics of the students regarding gender, age, class, parents’ education, ascariasis, nutritional and anemic status. Then, the data were further analyzed to determine the association of ascariasis with nutritional and anemic status of the students using the chi square test or Fisher test if the requirement was not fulfilled.Results: Sixteen (22%) students were having Ascaris lumbricoides infection, six (8.1%) students were thin and seventeen (23%) students were anemic. There was no statistically significant association found of Ascaris lumbricoides infection with nutritional and anemic status in early school-age students (P value <0.05) in this study.Conclusions: There is no statistically significant association of Ascaris lumbricoides infection with nutritional and anemic status of the early school-age students. [AMJ.2016;3(1):93–8] DOI: 10.15850/amj.v3n1.710
Association between Mothers’ Characteristics, Knowledge, Attitude, and Practice and Intestinal Helminthes Infection on Children Nadhira Permata Hakiki; Lia Faridah; Meita Dhamayanti
Althea Medical Journal Vol 3, No 2 (2016)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (134.874 KB)

Abstract

Background: The prevalence of soil-transmitted helminthes infection in Indonesia is still high, especially in children aged 3 to 8 years old. Helminthes infection cause loss of nutrition, delay physical development, intelligence, and labor productivity and decrease immunity. Mothers’ characteristics, knowledge, attitude, and practice are some of the factors that influence the occurrence of intestinal helminthes infection on children. This study was aimed to find the association between mothers’ characteristics, knowledge, attitude, and practice and the occurrence of intestinal helminthes infection on children.Methods: The study was conducted at Jatinangor Cohort’s research center from August to September 2014 using cross sectional analysis. One hundred and forty five secondary data were collected using validated questionnaire that filled by mothers and results of feces on children were tested. The amount of data excluded due to incomplete was 8, the data utilized was then analyzed by Chi Square evaluation.Results: Mothers’ characteristics such as age (P = 0.611), education (P = 0.952), occupation (P = 0.876), income (P = 0.199), and knowledge (P = 0.424; OR = 1.333), attitude (P = 0.236; OR = 0.808), practice (P = 0.333; OR = 4.625) did not have a significant association with the occurrence of intestinal helminthes infection on children.Conclusions: Characteristics, knowledge, attitude, and practice of the mothers towards the intestinal helminthes infection do not associate with the occurrence of intestinal helminthes infection on children. [AMJ.2016;3(2):248–53] DOI: 10.15850/amj.v3n2.794
Semi-quantitative Digital Analysis for Human Papillomavirus Detection from Environmental Specimens Adelina Siagian; Dicky Bagus Pratama; Fahmy Fathurrohman; Lia Faridah; Savira Ekawardhani
Althea Medical Journal Vol 7, No 3 (2020)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v7n3.1918

Abstract

Background: Recently, human papillomavirus (HPV) deoxyribonucleic acid (DNA) has been detected in urban wastewater, indicating that the virus can reach the sewer and, eventually, other water environments. This study aimed to develop a semi-quantitative assay for HPV DNA detection from environmental specimens using the PCR gel electrophoresis method.Method: This was an experimental descriptive qualitative study conducted from July to November 2019 in a standard molecular laboratory and non-laboratory administration room without air conditioner. Three brands of PCR reagents and different annealing temperatures were compared to identify the best condition for conventional PCR of plasmid DNA containing the HPV L1 gene. The semi-quantitative data were obtained from densitometry digital analysis using an imaging software. The optimized protocol was then applied on DNA serial dilutions to seek for the lower limit of detection (LLOD) value and the linear range of the assay. To evaluate the robustness of the assay, the protocol was further applied to spiked specimens of wastewater. Finally, several wastewater samples were tested for the presence of HPV DNA using this protocol.Results: A broad linear range and HPV L1 gene detection ability were observed with an LLOD of less than 2pg plasmid DNA in field condition. Although the assay successfully detected HPV DNA from several spiked wastewater samples, certain wastewater could interfere with the assay and gave false negative result.Conclusion: A semi-quantitative conventional PCR method to detect HPVDNA from environmental samples has been established and proven to be robust in field condition with non-optimum cold chain.
Identification of Giardia lamblia, Entamoeba histolytica and Cryptosporidium sp. in Feces of Diarrheal Patient at Puskesmas Jatinangor, September–November 2012 Komathi Palani; Lia Faridah; H. Uun Sumardi
Althea Medical Journal Vol 2, No 2 (2015)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (529.961 KB)

Abstract

Background: Diarrhea is one of the main public health problems occurring in West Java. One of the affected areas is Subdistrict Jatinangor. Inappropriate management of sanitation facilities around Jatinangor area causes contamination of water. Cikeruh River is one of the water sources in Jatinangor Area, from which people obtain water for daily activities. Water borne illness due to poor sanitation condition can lead to parasitic infection such as Giardia lamblia, Entamoeba histolytica and Cryptosporidium parvum whichcan cause a prolonged diarrhea. There has not been any study done regarding the presence of parasitical infection causing diarrhea around Jatinangor.Methods: In order to identify the parasitic infection, a descriptive study was carried out on 16 fecal samples collected from diarrheal patient who visited Puskesmas Jatinangor from September–November 2012. The parasites were checked by using wet mount methodResults: The parasites found were Entamoeba histolytica, Cryptosporidium parvum, but none of Giardia lamblia. There were also other findings such as Iodamoeba butschlii and Entamoeba coli.Conclusion: Positive findings of Entamoeba histolytica and Cryptosporidium parvum in diarrhea patients is most probably due to contaminated water and food. Measures need to be done to improve sanitary condition in Cikeruh River to prevent diarrhea. [AMJ.2015;2(1):213–16]
Diagnostic Value of Coproantigen for Detection of Giardia Infection in Stunted Children Octoviani, Fanny Anggraeni; Kurniawan, Agnes; Sari, Ika Puspa; Fauziah, Nisa; Faridah, Lia; Adrizain, Riyadi
Althea Medical Journal Vol 10, No 3 (2023)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v10n3.2860

Abstract

Background: Giardiasis is a protozoa infection caused by Giardia intestinalis, which commonly infects children, impairing children’s growth, development, and cognitive function. Standard diagnosis is carried out by microscopic examination of stool. This study aimed to evaluate coproantigen examination in stunted children compared to microscopic examination.Methods: A cross-sectional study was performed on stools collected from a survey among stunted children in Bandung in 2019. Stools were preserved in 10% formaldehyde and kept at -20oC until used. Direct microscopy examination with 2% lugol solution and coproantigen ELISA test using Giardia Cryptosporidium (combo test) coproantigen test kit were performed in Parasitology Laboratory Faculty of Medicine, Universitas Indonesia.Result: A total of 99 stools originated from stunted children aged 2-6 years. with boys predominant (52.5%). Microscopic examination showed that 12.1% (12/99) of the children were harboring intestinal parasites, such as the protozoa Giardia intestinalis, Blastocystis spp, and Entamoeba coli (E. coli). Giardia was the primary infection (9.1%), of which single Giardia infection (n = 8) and mixed infection of Giardia and Blastocystis spp (n = 1).  Interestingly, coproantigen examination resulted in 6 positive samples, and 4 samples agreed with the microscopy result. With a sensitivity of 44.4% and a specificity of 97.7%. The positive and negative predictive values were 66.7% and 94.7%, respectively.Conclusion: A moderate prevalence of Giardia in stunted children in Bandung regency has been observed. The combo coproantigen test method has high specificity and is suitable for use as a confirmation test to exclude Giardia infection.
Co-Authors Achadiyani Adams, Fayyaza Faiz Adelina Siagian Adrizain, Riyadi Agnes Kurniawan Agoes, Ridad Agrianfanny, Yukan Niko Ainul Yaqin Akbar, Muhamad Lazuardi Albert, Christian Amirul Mukminin Angga, Anggisti Nurdinda Chaerany Putri Ardini Raksanegara Arie Galih Mohamad Asep Sofyan Astri Gloria Larwuy Bashari, Muhammad Hasan Chin Annrie Eidwina Chin Annrie Eidwina, Chin Annrie Cica Lavemita Dicky Bagus Pratama Dida Akhmad Gurnida Dini Oktaviani Djati, Imam Damar Dwi Agustian Ekawardani, Savira Elsa, Zahratul Eva Nuriyah, Eva Fahmy Fathurrohman Fauzah, Nisa Fauziah, Nisa Fauziah, Nisa Gaga Irawan Nugraha H. Uun Sumardi H. Uun Sumardi, H. Uun Hadyana Sukandar Hadyana Sukandar Hakiki, Nadhira Permata Hamda, Muhammad Ersyad Hazel Faras Alhafiz Heni Djuhaeni Hesti Lina Wiraswati I Gede Nyoman Mindra Jaya I Made Joni Ida Kinasih Ika Puspa Sari Ita Krissanti Jontari Hutagalung Jontari Hutagalung Kamilah, Mutiara Mila Khotibul Umam Komathi Palani Komathi Palani, Komathi Latama, Zahra Nabila Leonita, Inggrid Mahira, Khansa Meita Dhamayanti Meita Dhamayanti Meri Alex Sandra Mufida, Hasna Muhammad Ersyad Hamda Muhammad Saifuddin B. S. Arif Nadhira Permata Hakiki Naufal Fakhri Nugraha Naufal Fakhri Nugraha Neneng Syarifah Syafei, Neneng Syarifah Nisa Fauziah Octoviani, Fanny Anggraeni PUTRI, RAFIKA Putri, Rafika Nanda Radiah Baizura Ramadhani Eka Putra raudatul jannah Reyhan Luthfierza Fauzan Riyadi Adrizain Rufinus, Wilbert Bernardi Safri Ishmayana Salma Nur Raidah Salsabila, Pricillia Laurenza Sarasati Windria Savira Ekawardhani Shabarni Gaffar -, Shabarni Gaffar Shabrinna, Hanif Sri Yusnita Irda Sari Sri Yusnita Irda Sari, Sri Yusnita Irda Sulaksono, Haura Labibah Salsabil Sunarjati Sudigdoadi Supriyadi, Isma Yustifania Syafrizal Saragih Titik Respati Uun Sumardi Uun Sumardi, Uun Virajati, Abimanyu Athallah Watanabe, Kozo Yasfira Aradella Yeni Wahyuni Hartati Yudith Setiati Ermaya Yuliarni, Dinda Zahra, Ridha Beta Zahratul Elsa