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All Journal Majalah Kedokteran Bandung ASPIRATOR Althea Medical Journal Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Riset Kimia Global Medical and Health Communication Molecular and Cellular Biomedical Sciences (MCBS) Chimica et Natura Acta Jurnal Endurance: Kajian Ilmiah Problema Kesehatan BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA) Jurnal Pengabdian Masyarakat (ABDIRA) Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)

Lia Faridah

Fakultas Kedokteran Universitas Padjadjaran

Author-ID : 532476

Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Economics, Econometrics & Finance Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Potential Zoonotic Gastrointestinal Nematode Infection from Goat in Sumedang Nisa Fauziah; Hazel Faras Alhafiz; Naufal Fakhri Nugraha; Ita Krissanti; Muhammad Ersyad Hamda; Lia Faridah
BALABA: JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA Volume 17 Nomor 1 Juni 2021
Publisher : Balai Penelitian dan Pengembangan Kesehatan Banjarnegara Badan Litbangkes Kemenkes RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/blb.v17i1.2671

Potential of zoonotic gastrointestinal nematode infection from livestock in Indonesia is still often overlooked. Farms with a risk for nematodes infection would create a risk of infecting the local community with zoonotic gastrointestinal nematodes. This study aimed to assess the risk of gastrointestinal nematodes from goats that have zoonotic potential in Cibeureum Wetan, Sumedang, and to identify the incidence of nematodes infection among goats. This was a cross-sectional study conducted in August to November 2019 with a total of 52 samples of feces collected directly from goat’s rectum to prevent soil contamination. Sampling was performed randomly from goats raised at the Agriculture and Self-Sustaining Village Training Center (Pusat Pelatihan Pertanian dan Pedesaan Swadaya, P4S) Simpay Tampomas, Sumedang, Indonesia. The GPS point of the sampling location was recorded. Samples were then examined using the concentration sedimentation method at the Parasitology Laboratory, Faculty of Medicine, Universitas Padjadjaran. Results showed that 22 of 52 samples were positive for gastrointestinal helminth eggs, contained of Bunostomum sp., Strongyloides sp., Haemmonchus sp., Trichostrongylus sp., Toxocara sp. and Trichuris sp. The nematode parasites found are parasites that often infect goats.

Knowledge of Helminthiasis of People Living in Slum Areas of Bandung District, Indonesia Lia Faridah; Nisa Fauziah; Riyadi Adrizain
Majalah Kedokteran Bandung Vol 53, No 4 (2021)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v53n4.2393

Among the most prevalent infections worldwide, Soil-Transmitted Helminth (STH) infection is the one that affects the poorest and most deprived populations. Data from the WHO shows that more than 1.5 billion people are affected by soil-transmitted helminth diseases globally. In 2010, it was estimated that 819.0 million, 464.6 million, and 438.9 million people around the world were infected with Ascaris lumbricoides, Trichuris trichiura, and hookworms (Ancylostoma duodenale and Necator americanus), respectively, with the Southeast Asia as the region with the highest prevalence of STH. Children’s awareness and knowledge of helminthiasis are essential to reduce the STH infection prevalence. Unfortunately, evaluation on children’s understanding of helminthiasis is still scarce. This study was intended to measure children's knowledge of STH infections and its prevention, especially prevention measures at home, in Bandung District, Indonesia. This study was conducted in March 2021 on 506 children who lived in 15 different subdistricts in Bandung District. Data were collected through a questionnaire that was used for face-to-face interviews with the children. All data were recorded in RedCap apps for further analysis. It was demonstrated in this study that children in Bandung District generally have low-to-medium knowledge on helminthiasis prevention, symptoms, and treatment (85%). Thus, there is an urgent need for an education program on helminthiasis to complement the deworming measures conducted by the local government.

Diagnostic Value of Coproantigen for Detection of Giardia Infection in Stunted Children Octoviani, Fanny Anggraeni; Kurniawan, Agnes; Sari, Ika Puspa; Fauziah, Nisa; Faridah, Lia; Adrizain, Riyadi
Althea Medical Journal Vol 10, No 3 (2023)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v10n3.2860

Background: Giardiasis is a protozoa infection caused by Giardia intestinalis, which commonly infects children, impairing children’s growth, development, and cognitive function. Standard diagnosis is carried out by microscopic examination of stool. This study aimed to evaluate coproantigen examination in stunted children compared to microscopic examination.Methods: A cross-sectional study was performed on stools collected from a survey among stunted children in Bandung in 2019. Stools were preserved in 10% formaldehyde and kept at -20oC until used. Direct microscopy examination with 2% lugol solution and coproantigen ELISA test using Giardia Cryptosporidium (combo test) coproantigen test kit were performed in Parasitology Laboratory Faculty of Medicine, Universitas Indonesia.Result: A total of 99 stools originated from stunted children aged 2-6 years. with boys predominant (52.5%). Microscopic examination showed that 12.1% (12/99) of the children were harboring intestinal parasites, such as the protozoa Giardia intestinalis, Blastocystis spp, and Entamoeba coli (E. coli). Giardia was the primary infection (9.1%), of which single Giardia infection (n = 8) and mixed infection of Giardia and Blastocystis spp (n = 1). Interestingly, coproantigen examination resulted in 6 positive samples, and 4 samples agreed with the microscopy result. With a sensitivity of 44.4% and a specificity of 97.7%. The positive and negative predictive values were 66.7% and 94.7%, respectively.Conclusion: A moderate prevalence of Giardia in stunted children in Bandung regency has been observed. The combo coproantigen test method has high specificity and is suitable for use as a confirmation test to exclude Giardia infection.

Effectiveness of Various Mosquito Attractant Solutions to Control Mosquito Population Faridah, Lia; Albert, Christian; Fauziah, Nisa
Global Medical & Health Communication (GMHC) Vol 7, No 1 (2019)
Publisher : Universitas Islam Bandung

The vector-borne disease is a disease caused by an organism that can transmit disease between human or animal to human. In Indonesia, several vector-borne diseases are a burden of the government including dengue fever, chikungunya, filariasis, and malaria. The attractive baited lethal ovitrap (ALOT) is a novel strategy to alleviate mosquito populations in three main actions: attraction, an adulticide, and larvacide. Research using plant infusion can attract mosquitoes to lay their eggs is needed. This study aims to compare the effectiveness of the mosquito repellent solution using materials from organic waste in Bandung. This study was a quantitative analytic study with a quasi-experimental design conducted in the Faculty of Medicine Universitas Padjadjaran area in October 2016–July 2017. Research subjects are mosquito eggs in a solution which placed at 25 different places for every solution. The analysis was performed using Kruskal-Wallis test followed by the Dunn test. The result of the Kruskal-Wallis test indicates the difference of effectiveness of each solution (p<0.05). Based on the results of the Dunn test, the most significant difference found in the solution of wood shavings with the vegetable waste solution and the solution of wood shavings with corn straw (p<0.05). In conclusion, there is a difference in the effectiveness of the mosquito repellent solutions and the most attractive solution for mosquitoes to oviposit is the corn straw solution. EFEKTIVITAS BERBAGAI LARUTAN PENARIK NYAMUK UNTUK MENGONTROL POPULASI NYAMUKPenyakit tular vektor adalah penyakit yang disebabkan oleh organisme yang dapat mentransmisikan penyakit antarmanusia atau hewan ke manusia. Di Indonesia, terdapat beberapa penyakit tular vektor yang masih menjadi beban pemerintah, di antaranya demam berdarah, chikungunya, penyakit kaki gajah, dan malaria. Attractive baited lethal ovitrap (ALOT) merupakan strategi baru untuk menurunkan populasi nyamuk dalam tiga aksi utama, yaitu attraction, adulticide, dan larvacide. Penelitian terkait larutan dari tanaman yang dapat menarik nyamuk sangat diperlukan. Penelitian ini bertujuan membandingkan efektivitas larutan penarik nyamuk dengan menggunakan bahan dari limbah organik yang ada di Kota Bandung. Penelitian ini adalah penelitian analitik kuantitatif dengan desain quasi-eksperimental yang dilakukan di lingkungan Fakultas Kedokteran Universitas Padjadjaran pada bulan Oktober 2016–Juli 2017. Subjek penelitian merupakan telur nyamuk yang ada pada larutan yang diletakkan pada 25 titik untuk setiap larutan. Analisis dengan Uji Kruskal-Wallis yang dilanjutkan dengan Uji Dunn. Hasil penelitian dengan Uji Kruskal-Wallis menunjukkan perbedaan efektivitas tiap-tiap larutan (p<0,05). Berdasar atas hasil Uji Dunn, perbedaan yang paling signifikan terdapat pada larutan serutan kayu dengan larutan sampah sayur dan larutan serutan kayu dengan jerami jagung (p<0,05). Simpulan, terdapat perbedaan efektivitas larutan penarik nyamuk dan larutan yang menarik nyamuk paling banyak untuk bertelur adalah larutan jerami jagung.

Arbovirus Detection of Adult Female Aedes aegypti for Dengue Surveillance: a Cohort Study in Bandung City, Indonesia Faridah, Lia; Ekawardhani, Savira; Fauziah, Nisa; Djati, Imam Damar; Putra, Ramadhani Eka; Watanabe, Kozo
Global Medical & Health Communication (GMHC) Vol 11, No 3 (2023)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v11i3.12749

Dengue surveillance is an important activity to prevent dengue outbreaks. This activity becomes a significant challenge for the region with limited logistic capabilities. Developing a simple mathematical model to predict the possibility of dengue incidence provides a reliable early warning system. This study compared the correlation between vector (adult female Aedes aegypti) and arbovirus detection on a vector to dengue incidence, which generalized linear mixed models tested. The incidence of adult female Aedes aegypti and dengue fever cases were interpolated through third-power inverse distance weighting (IDW). A spatial correlation between female Aedes aegypti incidence and dengue incidence was obtained from polynomial regression. Collection sites were 16 villages in Bandung city, one of the significant dengue endemic areas in January–December 2017. A total of 8,402 mosquitoes of Aedes aegypti, Aedes albopictus, and Culex sp., with 17% belonging to Aedes aegypti as the subject of the dengue virus (DENV) infection test. Data analysis only showed a weak correlation between the numbers of adult female Aedes aegypti and dengue incidence. On the other hand, there is no correlation between positive dengue infection of vector and dengue incidence. This study highlights the importance of constant arbovirus surveillance and integrated surveillance methods on all possible dengue vectors to develop an early warning system for dengue incidence.

Stakeholder Insights on Malaria Elimination Strategies in Pangandaran, West Java: a Qualitative Analysis Faridah, Lia; Fauziah, Nisa; Adams, Fayyaza Faiz; Mufida, Hasna; Akbar, Muhamad Lazuardi; Salsabila, Pricillia Laurenza; Rufinus, Wilbert Bernardi; Virajati, Abimanyu Athallah; Angga, Anggisti Nurdinda Chaerany Putri; Mahira, Khansa; Zahra, Ridha Beta
Global Medical & Health Communication (GMHC) Vol 12, No 2 (2024)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v12i2.13483

Indonesia has launched a determined effort to eliminate malaria by 2023, focusing on Java and Bali. Despite these efforts, Pangandaran in Java still faces malaria cases. This study aims to meticulously delve into the intricacies of the malaria elimination program's implementation while conducting a comprehensive evaluation of its effectiveness. Structured interviews were conducted with key stakeholders responsible for the malaria program in Pangandaran to extract invaluable insights. The study, carried out in November 2022, followed qualitative research with narrative analysis to reveal nuanced narratives from the participants. Findings from this rigorous analysis revealed a harmonious alignment between the malaria control program in Pangandaran and the Indonesian Ministry of Health guidelines. The strategy to combat malaria vectors in Pangandaran included mosquito net distribution, strategic larvicide application, and educational campaigns like Malaria Awareness Society (MASAMA). The expectation is that the current effective control program will resonate within the Pangandaran community, ultimately leading to the realization of the 2023 elimination target.

Cytotoxicity Effect of Aqueous Propolis Extract of Geniotrigona thoracica Sumatrans on Colo-201 Colon Cancer Cell Line and Senescence Colo-201 Colon Cancer Cell Line Induced by Low-dose Doxorubicin Latama, Zahra Nabila; Achadiyani, Achadiyani; Kamilah, Mutiara Mila; Putra, Ramadhani Eka; Kinasih, Ida; Faridah, Lia; Ekawardhani, Savira
Global Medical & Health Communication (GMHC) Vol 12, No 2 (2024)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v12i2.13760

Propolis, a resinous compound honeybees produce, demonstrates an extensive spectrum of powerful biological properties. However, the anti-cancer activity of propolis derived from Geniotrigona thoracica Sumatrans has yet to be reported. Thus, we sought to investigate the cytotoxicity of aqueous propolis extracts from Geniotrigona thoracica Sumatrans against Colo-201 colon cancer cell line and senescence Colo-201 colon cancer cell line induced by low-dose doxorubicin. This study was conducted at the Parasitology Laboratory of Human, Safety, and Environment, Universitas Padjadjaran Bandung from January to May 2024. This study assessed cell viability using the WST-1 test. Non-induced Colo-201 cells were treated with an aqueous extract of propolis (AEP) 100 ppm, or 5-fluorouracil (5-FU) 5 mg/ml as the positive control or water as a vehicle on untreated control. Colo-201 senescence was induced by doxorubicin 0.1 µM for three days. Doxorubicin-induced Colo-201 senescence was then treated with AEP 100 ppm, with 5-FU 5 mg/ml as the positive control, or with the combination of AEP 100 ppm and 5-FU 5 mg/ml, or water as a vehicle on untreated control. The data were analyzed using SPSS version 25.0, a one-way ANOVA, and Tukey’s post hoc test. The results showed that AEP has cancer-killing effects on Colo-201 cells and Colo-201 senescent cells induced by low-dose doxorubicin. AEP-treated Colo-201 cells and Colo-201 senescent cells induced by low-dose doxorubicin viability were significantly reduced to 37.15% and 13.72%, respectively, although slightly higher than those of the 5-FU-treated one at this concentration. There was also a decrease in the cancer-killing effect of 5-FU from 88.55% in non-induced Colo-201 cells to 41.5% in the doxorubicin-induced Colo-201 senescence model. In conclusion, aqueous extract of propolis from Geniotrigona thoracica Sumatrans showed cancer-killing-effects both on the Colo-201 colon cancer cell line and senescence Colo-201 colon cancer cell line induced by low-dose doxorubicin.

Promotion of Crypt-like Structures in Intestinal Organoid Development through the Addition of Graphene Oxide in Cell-based Assays Sulaksono, Haura Labibah Salsabil; Kamilah, Mutiara Mila; Faridah, Lia; Joni, I Made; Watanabe, Kozo; Ekawardhani, Savira
Global Medical & Health Communication (GMHC) Vol 12, No 3 (2024)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v12i3.13386

The intestinal organoid represents a miniature organ that can mimic functional physiology and pathology. However, there are several challenges to developing the organoid system, such as the limited survival of cells. Based on theory, matrix addition is a factor that can support survival in cells. As a result, graphene oxide (GO) addition is used in this study. As an artificial matrix, GO has been successfully shown to encourage good cell behavior and is well known for having good biocompatibility. Herein, we fabricate GO characterized with FT-IR and PSA. Crypt-like structures (CLS) are isolated from small intestinal mice in GO addition as a matrix. The gene expression and cell viability of CLS are investigated. RT-PCR examined the gene expression in CLS, while cell viability of CLS was carried out using the staining method. This study was conducted at FiNder U-CoE and Parasitology Laboratory of HSE Universitas Padjadjaran Bandung during February and December 2023. Our results show that Vil-1 as an identity for cells in the intestinal epithelium has been expressed in CLS primary significantly higher than intestinal tissue (p=0.01). However, identifying Lgr5 in CSL isolates is tricky. Thes in the crypt may be limited. Besides that, cell viability of CLS with GO addition can be maintained for four days. The GO addition as a matrix may provide support to maintain CLS. These findings are promising as cell-based assays for developing organoid models.

The μDrop Method Enhances Melanin Content Measurement in the in vitro Melanogenesis Model Using B16F10 Cell Line Yuliarni, Dinda; Kamilah, Mutiara Mila; Nugraha, Gaga Irawan; Faridah, Lia; Bashari, Muhammad Hasan; Ekawardhani, Savira
Molecular and Cellular Biomedical Sciences Vol 9, No 1 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i1.527

Background: The B16F10 cell line is a cell frequently used in melanin content assays. However, reports on cell models using B16F10 are limited, particularly as the robust model cell in the Indonesian cosmetics industry. We found measuring melanin content using microplate spectrophotometry to be challenging, so this research was conducted to develop a method using μDrop spectrophotometry.Materials and methods: In this in vitro study, the B16F10 melanoma cell line was cultured in Roswell Park Memorial Institute (RPMI) medium containing 5% fetal bovine serum (FBS). The cells were categorized into control, stimulated, and treated groups. Melanogenesis stimulation was achieved using 1μM α-melanocyte-stimulating hormone (α-MSH), while inhibition using 800 μg/ml kojic acid. After treatment, the cells were incubated for 48 hours. Their melanin content was then measured using an ELISA reader with a μDrop method and compared with the microplate method. Statistical analysis used a one-way ANOVA test with Turkey’s Post Hoc analysis.Results: The μDrop method increased the melanin signal into the linear range of machine readings, while the signals from the microplate method fell far below this range. The B16F10 melanoma cell lines stimulated by α-MSH exhibited increased melanin production compared with the control group, while kojic acid treatment significantly reduced (p<0.05) melanin content in the stimulated group.Conclusion: The μDrop method significantly outperformed the microplate method in measuring melanin content within melanogenesis cell models, offering enhanced accuracy and particularly excelling at quantifying low content of melanin. Keywords: μDrop, microplate, melanin, melanogenesis, B16F10 cell line, RPMI

New Custom Primers for the Detection of SARS-CoV-2 using the Singleplex rRT‒PCR SYBR Green-Based Method with the NSP10 and N genes as Targets Gaffar, Shabarni; Shabrinna, Hanif; Putri, Rafika; Wiraswati, Hesti Lina; Hartati, Yeni Wahyuni; Ishmayana, Safri; Faridah, Lia; Ekawardhani, Savira
Chimica et Natura Acta Vol 13, No 1 (2025)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v13.n1.53493

Although COVID-19 is no longer a global health emergency, rapid, sensitive, and specific detection tests are still needed. In this study, we developed a cost-effective test, the SYBR Green-based rRT‒PCR kit, using new custom primers targeting the N and NSP10 genes of the SARS-CoV-2 virus. The specificity of the designed primers was determined through agarose gel electrophoresis. A standard curve generated from a ten-fold dilution of SARS-CoV-2 RNA was used to determine the efficiency and sensitivity of the kit. Validation of this protocol was carried out on ten clinical specimens. As expected, the results showed that the N and NSP10 gene primers produced 134 and 161 bp products, respectively. The limits of detection and limit of quantification with N gene primers were 7.74 and 23.46 copies/μL, respectively, and those with the NSP10 gene primers were 4.69 and 14.21 copies/μL, with a PCR efficiency of 102.5% and 110.6%, respectively. The validation results with clinical specimens revealed that seven samples were true-positive for COVID-19 (Ct range 15.09–21.33), and three were confirmed to be true-negative. Costs associated with COVID-19 patient testing can be anticipated to decrease with the use of custom primers for the detection of SARS-CoV-2 via the use of the singleplex rRT‒PCR mix SYBR Green.

Co-Authors Achadiyani Adams, Fayyaza Faiz Adelina Siagian Adrizain, Riyadi Agnes Kurniawan Agoes, Ridad Agrianfanny, Yukan Niko Ainul Yaqin Akbar, Muhamad Lazuardi Albert, Christian Amirul Mukminin Angga, Anggisti Nurdinda Chaerany Putri Ardini Raksanegara Arie Galih Mohamad Asep Sofyan Astri Gloria Larwuy Bashari, Muhammad Hasan Chin Annrie Eidwina Chin Annrie Eidwina, Chin Annrie Cica Lavemita Dicky Bagus Pratama Dida Akhmad Gurnida Dida Akhmad Gurnida, Dida Dini Oktaviani Djati, Imam Damar Dwi Agustian Dwi Agustian Ekawardani, Savira Elsa, Zahratul Eva Nuriyah, Eva Fahmy Fathurrohman Fauzah, Nisa Fauziah, Nisa Fauziah, Nisa Gaga Irawan Nugraha H. Uun Sumardi H. Uun Sumardi, H. Uun Hadyana Sukandar Hadyana Sukandar Hakiki, Nadhira Permata Hamda, Muhammad Ersyad Hazel Faras Alhafiz Heni Djuhaeni Hesti Lina Wiraswati I Gede Nyoman Mindra Jaya I Made Joni Ida Kinasih Ika Puspa Sari Ita Krissanti Jontari Hutagalung Jontari Hutagalung Kamilah, Mutiara Mila Khotibul Umam Komathi Palani Komathi Palani, Komathi Latama, Zahra Nabila Leonita, Inggrid Mahira, Khansa Meita Dhamayanti Meita Dhamayanti Meri Alex Sandra Mufida, Hasna Muhammad Ersyad Hamda Muhammad Saifuddin B. S. Arif Nadhira Permata Hakiki Naufal Fakhri Nugraha Naufal Fakhri Nugraha Neneng Syarifah Syafei, Neneng Syarifah Nisa Fauziah Octoviani, Fanny Anggraeni PUTRI, RAFIKA Putri, Rafika Nanda Radiah Baizura Ramadhani Eka Putra raudatul jannah Reyhan Luthfierza Fauzan Riyadi Adrizain Rufinus, Wilbert Bernardi Safri Ishmayana Salma Nur Raidah Salsabila, Pricillia Laurenza Sarasati Windria Savira Ekawardhani Shabarni Gaffar -, Shabarni Gaffar Shabrinna, Hanif Sri Yusnita Irda Sari Sri Yusnita Irda Sari, Sri Yusnita Irda Sulaksono, Haura Labibah Salsabil Sunarjati Sudigdoadi Supriyadi, Isma Yustifania Syafrizal Saragih Titik Respati Uun Sumardi Uun Sumardi, Uun Virajati, Abimanyu Athallah Watanabe, Kozo Yasfira Aradella Yeni Wahyuni Hartati Yudith Setiati Ermaya Yuliarni, Dinda Zahra, Ridha Beta Zahratul Elsa

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