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All Journal Journal of Tropical Soils The Journal of Experimental Life Sciences (JELS) Buletin Penelitian Tanaman Rempah dan Obat Research Journal of Life Science Indonesian Green Technology Journal AGRIVITA, Journal of Agricultural Science Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Buletin Penelitian Tanaman Rempah dan Obat PERTANIAN TROPIK Jurnal HPT (Hama Penyakit Tumbuhan)
Syamsuddin Djauhari
Jurusan Hama Dan Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Engineering Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Other

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Sugarcane streak mosaic virus (ScSMV) Resistance Evaluation of Sugarcane Varieties Ernawati, Feny; Djauhari, Syamsuddin; Martosudiro, Mintarto; Putra, Lilik Koesmihartono; Kristini, Ari
Jurnal Perlindungan Tanaman Indonesia Vol 26, No 2 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.71158

Abstract

Sugarcane streak mosaic virus (ScSMV) is the most important viral disease of sugarcane in Indonesia with distribution in almost all commercial sugarcane plantations. The disease causes significant yield losses of both cane tonnage and sugar yield. The use of resistant varieties is the best approach for controlling viral diseases. This study aims to investigate resistance response of several introduced varieties against ScSMV in a glasshouse condition and the impact of the viral infection on chlorophyll and proline content in sugarcane leaves.  Sugarcane plants were inoculated using ScSMV inoculum one month after planting using an abrasive pad rubbing method. Disease incidence and severity was observed at week 4-12 after inoculation and variety resistance levels were classified based on disease incidence. Confirmation of the virus was done by RT-PCR. Spectrophotometer was used to measure chlorophyll content at dual wavelengths of 645 and 663 nm, and proline content at wavelengths of 520 nm. The results showed that most of the tested varieties were susceptible to ScSMV. There are six highly resistant varieties, namely SRA 1, SRA 2, N 10-4, N 10-7, N10-9, and N 10-13, but these varieties still require to be tested on a field scale. ScSMV infection generally decrease chlorophyll and proline content. However, the physiological effect of ScSMV infection on chlorophyll and proline content needs further investigation.
The Role of Indigenous Mycorrhiza in Combination with Cattle Manure in Improving Maize Yield (Zea Mays L) on Sandy Loam of Northern Lombok, Eastern of Indonesia Astiko, Wahyu; Sastrahidayat, Ika Rochdjatun; Djauhari, Syamsuddin; Muhibuddin, Anton
JOURNAL OF TROPICAL SOILS Vol. 18 No. 1: January 2013
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2013.v18i1.53-58

Abstract

A glass house study was conducted to evaluate the contribution of indigenous arbuscular mycorrhiza fungi (AMF) in improving maize yield grown on sandy loam of Northern Lombok. The package of organic fertilizers treatments were tested including: without inoculation of mycorrhiza, inoculation mycorrhiza and no added inorganic fertilizers, inoculation of mycorrhiza with cattle manure added, inoculation of mycorrhiza with rock phosphate added and inoculation mycorrhiza with inorganic fertilizers. The treatments were arranged using a Completely Randomized Design with four replications. The results of the study show that the inoculation of AMF significantly increased soil concentration of N, available-P, K and organic-C by 37.39%, 60.79%, 66.66% and 110.15% respectively observed at 60 days after sowing (DAS). The similar trend was also found at 100 DAS, where those nutrients increased by 21.48%, 69%, 43.93% and 37.07%, respectively compared to control. The improving of soil fertility status was also reflected by nutrients uptake (i.e. N, P, K, Ca) as well as growth and yield of maize. N, P, K and Ca uptake increased by 1,608%, 1,121%, 533% and 534%, respectively. Roots and top dry biomass at 60 DAS increased by 718.40% and 337.67%, respectively. The trend increased of the biomass was followed by observation at 100 DAS. Yield components including cobs, grain and weight of 100 grains increased by 313.60%, 411.84% and 137.54%, respectively. In addition, the inoculation of AM with F2 contributed significantly to the spore numbers and root infection.[How to Cite : Astiko W, IR Sastrahidayat, S Djauhari, and A Muhibuddin. 2013. The Role of Indigenous Mycorrhiza in Combination with Cattle Manure in Improving Maize Yield (Zea Mays L) on Sandy Loam of Northern Lombok, Eastern of Indonesia. J Trop Soils, 18 (1): 53-58. doi: 10.5400/jts.2013.18.1.53][Permalink/DOI: www.dx.doi.org/10.5400/jts.2013.18.1.53]
Control of "Damping Off" Disease Caused by Sclerotium Rolfsii Sacc. using Actinomycetes and Vam Fungi on Soybean in The Dry Land Based on Microorganism Diversity of Rhizosphere Zone Rochjatun, Ika; Djauhari, Syamsuddin; Saleh, Nasir; Muhibuddin, Anton
AGRIVITA Journal of Agricultural Science Vol 33, No 1 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i1.37

Abstract

One of the obstacles in the efforts to increase soybean production in Indonesia is disease such as damping off which is caused by Sclerotium rolfsii. In East Java, the intensity of S. Rolfsii reached approximately 8.61% that spread all over Indonesia region, even in our neighbor countries such as Malaysia, Thailand and the Philippines. This research was carried out to determine the efficacy of Actinomycetes and VAM (Vesicular Arbuscular Mycorrhizal) against damping-off attack and the diversity of microorganisms in rhizosfer. Research conducted in the laboratory and screen house on Plant Protection Department, Faculty of Agriculture, University of Brawijaya and in Lawang District Malang. Observation variables include level of pathogen attack and infection rate of dampingoff pathogen. Plant height, number of pods, pod weight, seed weight and weight of 100 seeds from each treatment, diversity and identification of microorganisms in rhizosphere were also observed. The results showed that Actinomycetes and VAM application could decrease the percentage of plant death due to damping-off. Application of Actinomycetes and VAM gave effect on microorganism diversity of Ratai Rhizosphere but not on Wilis. Keywords: Sclerotium rolfsii, vesicular arbuscular mycorrhizal, actinomycetes, and rhizosphere
ARBUSCULAR MYCORRHIZA FUNGI AS AN INDICATOR OF SOIL FERTILITY Syibli, Muhammad Akhid; Muhibuddin, Anton; Djauhari, Syamsuddin
AGRIVITA Journal of Agricultural Science Vol 35, No 1 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i1.228

Abstract

Arbuscular mycorrhizal fungi (AMF) are ubiquitous organism that forms association with the root of most terrestrial plants. AMF association also influence soil fertility through the enhancement of chemical, biological and physical content. In this study, we enumerated AMF spores from rhizosphere of Tithonia difersivolia as an indicator of soil fertility. The results showed that the most fertile soil had the highest AMF spores density. This research has confirmed that AMF has high interaction with organic carbon, organic matter, total phosphorus, cation exchange capacity, water level, soil fungi and soil bacteria. Partial regression analysis revealed the mathematic equation for their interaction. This equation used the abundant of AMF spores as an indicator for chemical, biological and physical fertility of the soil.                 Keywords: Arbuscular mycorrhiza fungi (AMF), indicator, soil fertility, Tithonia difersivolia  
Pathogenicity, Development and Reproduction of The Entomopathogenic Nematode Steinernema sp., in Mealworm Tenebrio molitor Baliadi, Yuliantoro; Sastrahidayat, Ika Rochdjatun; Djauhari, Syamsuddin; Rahardjo, Bambang Tri
AGRIVITA Journal of Agricultural Science Vol 33, No 3 (2011)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v33i3.74

Abstract

The pathogenicity, development and reproduction of Steinernema sp., isolate Skpr-20/Str, were studied using Tenebrio molitor. Results revealed that pathogenicity, development and reproduction were significantly influenced by nematode doses. Although the number of invading IJs increased with increasing dose, percentage penetration declined. The IJs reached adulthood within 3 days. Females laid eggs from day 4-7. All eggs remaining inside uterus develop inside the maternal body. The first female bearing endotokia matricida was observed on day 5. In a sand-based assay, nematode was more pathogenic at lower dose instead of higher ones, where optimum dose was 80 nematodes per larva and average number of progeny per female was 5438. Under crowded conditions, development proceeds to IJ stage instead of the J3. The average length and width decreased with increasing of nematode doses. The IJ produced in cadavers infested with 640 nematodes per larva was significantly smaller (492 ± 6.4 µm) than offspring from other doses. The number of days which nematodes first emerged from the cadavers decreased with increasing dose. IJ first emerged at the average of 10-13 days at high IJ densities. It is concluded that the wide experimental characteristic of EPNs is also true for Steinernema sp., isolate Skpr-20/Str.Keywords: development, emergence period, endotokia matricida, entomopathogenic nematode, isolation, pathogennicity, Steinernema sp., isolate Skpr- 20/Str
SOIL FERTILITY STATUS AND SOYBEAN [Glycine max (L) Merr] PERFORMANCE FOLLOWING INTRODUCTION OF INDIGENOUS MYCORRHIZA COMBINED WITH VARIOUS NUTRIENT SOURCES INTO SANDY SOIL Astiko, Wahyu; Sastrahidayat, Ika Rocdjatun; Djauhari, Syamsuddin; Muhibuddin, Anton
AGRIVITA Journal of Agricultural Science Vol 35, No 2 (2013)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v35i2.249

Abstract

The experiment tested four treatments, namely inoculation of soil with mycorrhiza, inoculation of soil with mycorrhiza and cattle manure, inoculation of soil with mycorrhiza and rock phosphate, and inoculation of soil with mycorrhiza and inorganic fertilizers. Soil without any inoculation was provided as control. The treatments were arranged in a Completely Randomized Design with four replications. Soil fertility status was based on the concentrations of N, P, K, and organic-C as well as soil pH. Plant performance was determined based on its ability to uptake nutrients (N, P, K, and Ca), its growth and yield. Then, the activity of mycorrhiza was measured based on total population and percentage of root infection. Overall, results of the present study showed that, compared with other treatments, inoculation of mycorrhiza into soil and amended with cattle manure significantly performed higher concentrations of N, P, K, and organic-C. This soil condition caused soybean to absorb significantly higher nutrients, grew well with higher yield compared with plant performance in other treatments. Therefore, results of this study implies that the application of mycorrhiza into soil amended with organic matter is promising to sustain soil productivity under soybean cropping system.  Keywords: Arbuscular Mycorrhiza, soil fertility, soybean yield, dryland
Co-Authors Abdul Cholil Abdul Cholil Abdul Cholil Abdul Cholil Abdul Latief Abadi Ade Supriyadi Ade Supriyadi Ahmad Eri Wirawan Ahmad Eri Wirawan Ahmad Ilham Tanzil Akbar, Faishal Anella Retna Kumala Sari Antok Wahyu Sektiono Anton Muhibuddin Anton Muhibuddin Ari Kristini Asri Ismahmudi Astri Septiyaningsih Nugraheni Astri Septiyaningsih Nugraheni Bambang Tri Rahardjo Desi Herawati Devia Enitasepa Dita Aprilia Mayasari Edi Priyo Utomo Edi Priyo Utomo Edi Priyo Utomo Eko Famuji Ariyanto Eko Famuji Ariyanto Ernawati, Feny Fatkur Roziq Fatkur Roziq Firdaus Auliya Rahmah Ika Rocdjatun Sastrahidayat Ika Rochdjatun Sastrahidayat Ika Rochdjatun Sastrahidayat Ika Rochjatun Imam Chanif Imam Chanif, Imam Jasuli, Jasuli Khoirunisa, Fenti Rahma Liliek Sulistyowati Lilik Koesmihartono Putra Lilik Sulistyowati Lilik Sulistyowati Luqman Qurata Aini Maulidatur Rahmawati Ningrum Maulidatur Rahmawati Ningrum Mintarto Martosudiro Muhammad Akhid Syibli Muhammad Arsys Tawa Muhibuddin, Anton Nasir Saleh Nia Devita Mevianti Nur Latifahani Oktavia Shinta Dwiyana Putri Oktavia Shinta Dwiyana Putri Pandu Indra Pratama Putri Setya Rahmita Redha Qadiani Ariyono Redha Qadiani Ariyono Rezyta Tri Yuli Hapsari Rezyta Tri Yuli Hapsari Ria Kurniasih Sarjoko Sarjoko Sektiono, Antok Wahyu Siti Aminatuz Zuhria Tijani Ahmad Wijaya Tijani Ahmad Wijaya Virgin Ayu Presty Mindarsusi Wahyu Astiko Wiwit Wicaksono Jati Yohana Avelia Sandy Yuliantoro Baliadi Yunita Dian Puspita