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All Journal Jurnal Online Mahasiswa (JOM) Bidang Keguruan dan Ilmu Pendidikan Indonesian Biology Teachers JMM (Jurnal Masyarakat Mandiri) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Ilmiah Wahana Pendidikan BIOGENESIS NUSRA: Jurnal Penelitian dan Ilmu Pendidikan Journal Of Human And Education (JAHE) JURNAL BIOLOGI PAPUA The International Journal of Tropical Veterinary and Biomedical Research PESHUM
Nursal Nursal, Nursal
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Journal : JURNAL BIOLOGI PAPUA

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Ekstraksi DNA Nibung (Oncosperma tigillarium (Jack) Ridl.) Menggunakan Beberapa Jenis Buffer Terhadap Kualitas Genomik DNA Mahadi, Imam; Sayuti, Irda; Nursal, Nursal
Jurnal Biologi Papua Vol 16 No 2 (2024)
Publisher : Jurusan Biologi FMIPA Universitas Cenderawasih

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31957/jbp.3780

Abstract

The Nibung (Oncosperma tigillarium (Jack) Ridl.) is a plant that serves as the floral mascot of Riau and symbolizes the spirit of unity and brotherhood among its people. Genetic diversity research is essential for the conservation of this species, with DNA extraction being a critical initial step. This study compared three types of buffers: CTAB, Alkyl Benzene Sulfonate (ABS) surfactant detergent, and Sodium Laureth Sulfate (SLS) surfactant detergent. The aim of this research was to identify alternative buffers to replace CTAB and simplify the extraction of genomic DNA from Nibung. Parameters measured included the presence of DNA and DNA concentration, using electrophoresis and a nanodrop spectrophotometer. The results showed that clear genomic DNA bands were successfully obtained for 24 Nibung accessions, with only two accessions, Nibung2s and Nibung6s, showing smeared bands when using the SLS surfactant detergent buffer. The genomic DNA concentrations ranged from 526.2 to 3,829.2 ng/µl. These concentrations are suitable for use as DNA templates in further research. Thus, both ABS and SLS surfactant detergent buffers can be considered viable alternatives to CTAB for extracting genomic DNA from Nibung.  Key words: Buffer; DNA bands; DNA extraction; DNA Concentration; Extraction optimization.
Co-Authors Amran, Afitria Anggrahini, Fify Apriliani, Salsabila Rika Arnentis Arnentis, Arnentis Darmadi Darmadi Darmawati Darmawati Dipuja, Diah Anugrah Elya Febrita Faedah, Siti Nur Fajri Fajri Fathonah Fathonah Ferdian, Riyan Firdaus LN Fitra Suzanti Fitriani, Raisa Gani, Ridho Abdul Hac D, M. Ulil Harlis Harlis Helfita, Rani Herlina, Nur Husna, Rizqa Alfajri Imam Mahadi Irda Sayuti Irda, Sayuti Juliana, Fitri Junaidi, Annajmu Sasna L.N. Firdaus, L.N. Firdaus L.N., Firdaus Ma’ruf, Rizky Mariani Natalina Mariani Natalina L, Mariani Mariani Natalina Linggasari Mariani NL, Mariani NL Masitta Tanjung Miftahul Jannah Muhammad Hasan Qonita S, Sakinah Rama, Ance Gusti Rasyida Ulfa Suwarno, Nella Suwnodo, Suwnodo Suwondo Suwondo Utari, Melita Wahyuni, Resma Wan Syafii Yuslim Fauziah Yustina Yustina Zulfarina, Zulfarina