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All Journal HAYATI Journal of Biosciences Jurnal Sains dan Seni ITS BIOTROPIA - The Southeast Asian Journal of Tropical Biology IPTEK Journal of Proceedings Series Indonesian Journal of Chemistry Akta Kimia Indonesia Journal of Fibers and Polymer Composites Sewagati

Adi Setyo Purnomo

Departemen Kimia, Institut Teknologi Sepuluh Nopember

Author-ID : 542924

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Engineering Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Public Health Social Sciences Veterinary Other

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Pengaruh Campuran Ampas Tebu dan Sabut Kelapa Sebagai Media Pertumbuhan Alternatif Terhadap Kandungan Jamur Tiram (Pleurotus ostreatus) Ade Restuani Rahma; Adi Setyo Purnomo
Jurnal Sains dan Seni ITS Vol 5, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

Pengaruh campuran ampas tebu dan sabut kelapa sebagai media pertumbuhan alternatif terhadap kandungan nutrisi jamur tiram putih (Pleurotus ostreatus) telah diteliti. Variasi komposisi sabut kelapa dan ampas tebu yang digunakan adalah 25:75 (F1), 50:50 (F2), 75:25 (F3), 100:0 (F4) dan 0:100 (F5). Jamur tiram yang dihasilkan dilakukan uji secara fisik dan kandungan nutrisi. Pada komposisi F1 menghasilkan jamur dengan diameter (13,9 cm), ketebalan (5,01 cm), dan panjang (13,8cm) terbaik, sedangkan untuk massa yang paling besar dihasilkan pada komposisi F5 yaitu 79,5 g, dan jumlah tudung paling banyak dimiliki oleh komposisi F3 sebanyak 16 buah. Dari hasil analisa nutrisi komposisi F1 memiliki kandungan nutrisi yang relatif baik. Pada komposisi F1 mempunyai kadar air (84,10%) yang rendah.

Pengaruh Campuran Ampas Tebu dan Tongkol jagung sebagai Media Pertumbuhan terhadap Kandungan Nutrisi Jamur Tiram Putih (Pleurotus ostreatus) Robiatuz Zuniar; Adi Setyo Purnomo
Jurnal Sains dan Seni ITS Vol 5, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

Pengaruh campuran ampas tebu dengan tongkol jagung sebagai media tanam terhadap kualitas jamur tiram telah diteliti. Media tanam dibuat berupa campuran ampas tebu dan tongkol jagung dengan perbandingan komposisi yaitu 75:25 (R1), 50:50 (R2), 25:75 (R3), 100:0 (R4), dan 0:100 (R5). Jamur tiram segar yang dihasilkan selanjutnya dianalisis kualitasnya yang meliputi analisis fisik, kadar air dan kadar lemak. Analisis kadar air menggunakan metode termogravimetri, sedangkan kadar lemak menggunakan metode soxhletasi. Hasil analisa fisik jamur tiram menunjukkan bahwa komposisi R5 memiliki massa ratarata paling tinggi (79,46 g). Jumlah tudung paling banyak pada komposisi R4 (13 buah), sedangkan diameter tudung paling baik pada komposi R1 yaitu 10,76 cm. Kadar air dan lemak terendah pada komposisi R4 yaitu 86,2288% dan 0,1199%. Penelitian ini menunjukkan bahwa penggunaan media dengan komposisi perbandingan yang berbeda dapat mempengaruhi kualitas fisik dan kadar air dari jamur tiram yang dihasilkan.

Effects of Acetic Anhydride toward Degree of Substitution on Acetylation Method of Sago Starch (Metroxylon sp) from Papua Yuliya Andriani Nanggewa; Adi Setyo Purnomo; Surya Rosa Putra
IPTEK Journal of Proceedings Series No 1 (2015): 1st International Seminar on Science and Technology (ISST) 2015
Publisher : Institut Teknologi Sepuluh Nopember

Sago contains carbohydrates that are stored in the starch form. Starch is generally formed from two molecules of glucose polymer, namely amylose and amylopectin, which its composition varies for each type of starch [1]. The weakness of starch can be overcome through a modification of the functional properties of starch to expand sago starch. Chemical modification of starches can enlarge the range of certain starch physical properties of the parent starch [2] and enhance their use in a number of applications found in industrial processes and food manufacture. Chemical modification of starch can be performed by various methods such as acetylation. Generally, native starch has a low Degree of Substitution (DS) because of their limited degree or reaction on the granule surface. Increasing DS can be obtained by modification of native starch through acetylation reaction using few catalysts such as pyridine and NaOH. Several researchers have reported the effects of acetylation on many sort of starch sources such potato, corn, pea and cassava [3-5]. There are few studies about the effects of acetylation of starches with a wide range of amylose contents. We have carried out some work on effects of acetic anhydride concentration toward DS value of native and modified sago starch from Papua.

Pengaruh Penambahan Bacillus subtilis Pada Biodegradasi DDT Oleh Phlebia brevispora Adi Setyo Purnomo; Fajriah Fajriah
Akta Kimia Indonesia Vol 2, No 1 (2017)
Publisher : LPPM, Institut Teknologi Sepuluh Nopember

DDT merupakan pestisida yang bersifat persisten di lingkungan, dan memiliki resiko kesehatan bagi manusia. Pada penelitian ini, pengaruh penambahan bakteri Bacillus subtilis pada biodegradasi DDT oleh jamur Phlebia brevispora telah diteliti. B. subtilis ditambahkan pada volume 1, 3, 5, 7, dan 10 mL (1 ml ≈ 6,7 × 108 sel bakteri / ml kultur). Hasil degradasi DDT oleh P. brevispora tanpa penambahan bakteri adalah 64%. Degradasi tertinggi DDT diperoleh pada penambahan 10 mL bakteri sekitar 75% selama masa inkubasi 7 hari. DDD, DDE and DDMU terdeteksi sebagai produk metabolit. Penelitian ini menunjukkan bahwa penambahan B. subtilis dapat meningkatkan degradasi DDT oleh P. brevispora.

The Effect of Pseudomonas aeruginosa Addition on 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) Biodegradation by Brown-rot Fungus Fomitopsis pinicola Atmira Sariwati; Adi Setyo Purnomo
Indonesian Journal of Chemistry Vol 18, No 1 (2018)
Publisher : Universitas Gadjah Mada

Effect of addition of Pseudomonas aeruginosa on 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) biodegradation by Fomitopsis pinicola had been investigated. P. aeruginosa was added into F. pinicola culture at 1, 3, 5, 7 and 10 mL (1 mL ≈ 1.53 x 109 P. aeruginosa bacteria cells/mL culture). The addition of 10 mL of P. aeruginosa showed the highest DDT biodegradation approximately 68% during 7 days incubation in Potato Dextrose Broth (PDB) medium, which was higher than biodegradation of DDT by F. pinicola only (42%) at the same incubation time. 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane (DDD), 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE) and 1-chloro-2,2-bis(4-chlorophenyl)ethylene (DDMU) were detected as metabolites from DDT biodegradation by mixed cultures of F. pinicola and P. aeruginosa.

The Biotransformation and Biodecolorization of Methylene Blue by Xenobiotic Bacterium Ralstonia pickettii Adi Setyo Purnomo; Asranudin Asranudin; Didik Prasetyoko; Yulinar Dwi Nur Azizah
Indonesian Journal of Chemistry Vol 21, No 6 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.65806

The biotransformation and biodecolorization of methylene blue (MB) dye using the bacterium Ralstonia pickettii was investigated. This experiment was conducted in a nutrient broth (NB) medium after adding MB at 100 mg L–1 concentration. Approximately 98.11% of MB was decolorized after 18 h of incubation. In addition, the metabolic products detected by LC-TOF/MS were Azure A (AA), thionine, leuco-MB, and glucose-MB, which indicated the MB degradation through a reductase that attacked the heterocyclic central chromophore group present in the structure. Moreover, azure A and thionine fragments resulted from the N-demethylase enzyme that attacked the auxochrome group. Thus, this research was assumed to be the first scientific report suggesting the potential to use R. pickettii in the biodecolorization and biotransformation of dye waste, particularly MB.

Decolorization and Transformation of Synthetic Dye Methylene Blue by Brown-Rot Fungus Fomitopsis pinicola Adi Setyo Purnomo; Hamdan Dwi Rizqi; Aulia Ulfi; Refdinal Nawfa; Herdayanto Sulistyo Putro
Indonesian Journal of Chemistry Vol 22, No 2 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.69834

Methylene blue (MB) is a synthetic dye widely used in industries that is difficult to degrade in the environment due to its stability. Brown-rot fungus Fomitopsis pinicola is an organism that is known to be able to degrade some organic pollutants such as DDT, aldrin, dieldrin, and methyl orange dye. This study aimed to explore the ability of F. pinicola on MB biodecolorization, to identify metabolites and propose a biodecolorization pathway. F. pinicola was evaluated for MB biodecolorization on PDA agar and PDB liquid media. The metabolites were determined by using LCTOF/MS. The PDA agar medium's largest index decolorization (ID) was 0.915%. The MB decolorization in liquid PDB medium showed the highest percentage of decolorization of 92.56% at MB concentration of 100 mg/L after 14-days incubation. The analysis using LC-TOF/MS showed metabolites from MB biodecolorization, namely 3-amino-7-(methylamino) phenothiazin-5-ium (Azure C), 3-(dimethylamino)-7-(methylamino) phenothiazin-5-ium (Azure B), and 3,7-bis(dimethylamino)-4aH-phenothiazin-5-one. The MB degradation pathway was proposed through demethylation and oxidation reactions based on the detected product metabolites. These results indicated that F. pinicola is a suitable agent for biodecolorization of MB, and can potentially be used for bioremediation of MB waste in the environment.

Polyhydroxyalkanoates Production from Ralstonia Pickettii Bacteria: Structural and Mechanical Studies Asranudin Asranudin; Surya Rosa Putra; Adi Setyo Purnomo; Dalia Allouss; Holilah Holilah; Lisman Suryanegara; Alvin Romadhoni Putra Hidayat; Alvin Rahmad Widiyanto; Muchammad Tamyiz
Journal of Fibers and Polymer Composites Vol. 1 No. 2 (2022): Journal of Fibers and Polymer Composites
Publisher : Green Engineering Society

Bacterial Polyhydroxyalkanoates (PHAs) are a remarkably versatile category of biodegradable polymers with a variety of applications in the packaging, agricultural, biomedical, and pharmaceutical fields. In the present study, bacterial PHAs films are characterized by Fourier transform infrared (FTIR), Scanning electron microscope (SEM), Gas chromatography-mass spectroscopy (GC-MS), Differential scanning calorimetry (DSC), and Universal testing machine (UTM). It was found that almost 20% (w/w) of PHAs was produced from Ralstonia pickettii, and the five major types of the produced polymer were validated via FTIR analysis, i.e., 1046-1185 cm-1 (C-O stretching), 1723 cm-1 (C=O stretching), 2974-2926 cm-1 (CH3 and CH2 stretching) and 3450 cm-1 (OH stretching). The GC-MS chromatogram generated two main peaks, i.e., 2-butenoic acid methyl ester and 4-hexenoic acid methyl ester, at retention times of 4.62 min and 5.79 min, respectively. The main compounds of 2-butenoic acid methyl ester and 4-hexenoic acid methyl ester had percentage areas of 28% and 43%, respectively. Based GCMS analysis shows two monomer PHAs ie. 2-butenoic acid methyl ester and 4-hexenoic acid methyl ester, correspondingly. The blending of PHAs R. pickettii and PEG-400 positively decreases thermal properties and tensile strength and increases elongation at break.

Effect of the Coconut Coir (Cocos nucifera) as a Growth Medium for Pleurotus ostreatus (Oyster Mushroom) on Mineral and Vitamin B Contents Adi Setyo Purnomo; Atmira Sariwati; Sri Fatmawati; Faradita Eka Puspitasari
HAYATI Journal of Biosciences Vol. 30 No. 1 (2023): January 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.1.95-104

The oyster mushroom (Pleurotus ostreatus) is the most cultivated mushroom which its growth performance and nutritional composition depend on substrate types. Thus, this study investigated the effect of coconut coir on minerals and vitamins B contents in the oyster mushroom cultivation medium. The composition of coconut coir and Sengon’s sawdust as growth medium were variated at ratios of 4:0 (F1), 3:1 (F2), 2:2 (F3), 1:3 (F4), 0:4 (F5) (w/w). Furthermore, the mineral and vitamin B contents of the harvested oyster mushrooms were analyzed by ICPMS and LCMS, respectively. The highest minerals contents of potassium 26,909 mg/kg, 1,136 mg/kg of phosphorus, 313 mg/kg of magnesium, 4,346 mg/kg of calcium, 15.4 mg/kg for zinc, 2.07 mg/kg of copper, 0.623 mg/kg of Selenium were identified at F1, whereas the highest manganese was at F2 (15.3 mg/kg). Potassium was the highest mineral content in oyster mushrooms in all growth media, and low toxic minerals content of cadmium was detected between 0.015-0.058 mg/kg in all variant substrates. Lead (0.525 mg/kg) and mercury (0.012 mg/kg) was only detected at F1, while arsenic was detected at F4 (0.002 mg/kg) and F5 (0.029 mg/kg). LCMS analysis showed that vitamin B1 (Thiamine), B3 (niacin), B6 (pyridoxine) and B12 (cyanocobalamin) were detected in all growth media, while vitamin B5 (pantothenic acid) was only detected at F5. These results indicated that the P. ostreatus cultivation on coconut coir become valuable nutritional resources to alleviate malnutrition and help dispose of coconut coir in an environment-friendly manner.

Biodecolorization of Methylene Blue by Using Bacillus subtilis Immobilized into SA-PVA-Bentonite Matrix in Mineral Salt Medium and Non-nutritious Medium Alya Awinatul Rohmah; Adi Setyo Purnomo; Widiya Nur Safitri
Indonesian Journal of Chemistry Vol 22, No 6 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.76080

Industrial dye wastewater can potentially cause significant harm to organisms and the environment across the world. Methylene blue (MB) is a synthetic dye that can be found in wastewater. Immobilizing clay material and degradative bacteria into a carrier is the best way to remove MB. Therefore, this study aimed to immobilize Bacillus subtilis in sodium alginate (SA)-polyvinyl alcohol (PVA)-Bentonite for adsorption and degrading MB in nutritious mineral salt medium (MSM) and non-nutritious media. The result showed that SA-PVA-Bentonite-non-living B. subtilis beads (SPB-nBS) had the highest result in non-nutritious medium, approximately 88.89%. While in nutritious MSM, living B. subtilis addition into beads (SPB-BS) reached the highest MB removal, which was 94.31%. Nutritious MSM had a role as the sole carbon and energy for living B. subtilis. So, it could adsorb and degrade MB by its enzymatic system. The degradation products were predicted as C7H10N2O4S, C8H10N2O2 and C6H8N2O3S. Hence, this study indicated that a nutritious medium was the suitable medium for MB degradation. According to the SEM result, the bacteria spread and covered the beads. Furthermore, the adsorption kinetics and isotherms were also analyzed; SPB beads followed the pseudo-second order kinetic model and Langmuir isotherm.

Co-Authors A'in Qurrota A'yunin Abdillah, Salsabilah Putri Ade Restuani Rahma Adisya Salsabila Aldianita, Ananda Krisna Alvin Rahmad Widiyanto Alvin Romadhoni Putra Hidayat Alya Awinatul Rohmah Alya Awinatul Rohmah Ariati, Nafla Rahma Dian Asranudin Asranudin Asranudin Asranudin Asranudin Asranudin Asranudin Asranudin Atmira Sariwati Aulia Ulfi Ayundra, Rezania Azzahira, Sabira Badzlin Nabila Badzlin Nabilah Ceisar Andrian Putra Ciptoaji, Yusril Rizqi Citra ‘Aaqilatul Fa’aidah Dalia Allouss Diah Trisna Rahayu Lestari Didik Prasetyoko Dini Ermavitalini Djohana Tri Lois Moyo Dwi Lestari Egar Pamela Eka Pratiwi Yuniarti Erly Grizca Boelan Fadhil Adinata Fadlan, Arif Fahimah Martak Fahimah Martak Fajriah Fajriah Fanda Luvita Sari Faradita Eka Puspitasari Farid Kamal Muzaki Fazad, M. Haikhal Ferdy Muhammad Gading, Anjani Puspa Gunawan, Triyanda Halimah, Adedila Hamdan Dwi Rizqi Hamzah Fansuri Hamzah Fansuri Hendro Nurhadi Herdayanto Sulistyo Putro Herdayanto Sulistyo Putro Hery Purnobasuki Hibatullah, Faizah Holilah Holilah Ichiro Kamei Iman, Muhammad Ilham Nur Inayah Fitri Indana Madaniyah Tsani Indrapaksi, Tengku Ikhwan Alfajri Irma Citra Mayangsari Iwan Adi Indrawan Karina Harley Aulia Rizky Khulaida, Khoridatul Laili Khumaidah Laudy Nasya Yanuar Lavida Aisya Salvanora Lea Kristina Anggraeni Lisman Suryanegara Maulianawati, Diana Mawardani, Alfiyyana Nurrahma Muchammad Tamyiz Mufidah, Iftin Farekana Muhammad Lukman Hakim Muhammad Lukman Hakim, Muhammad Lukman Muhammad Rasyid Abdullah Nabilah Farah Azzahra Naila, Ishmatun Nawfal, Mohammad Fadl Johara Nela Rachmawati Nourma Sulistyowati Novia Nurul Hidayah Nur Khoiriyah Nurhayati, Alfia Nurlatifah Nurlatifah Nurul - Widiastuti Nurul Widiastuti Octaviani, Wulan Nur Oktafia, Galuh Krisna Pangayom, Danang Luhur Prasetiyowati, Mega Pratama, Silvia Abdi Pratiwi, Tasya Resa Putra, Ananda Satria Eka Putra, Muhammad Farhan Lucky Putri, Aulia Malihah Putri, Devi Anggraini Putro, Herdayanto Sulistyo Refdinal Nawfa Refdinal Nawfa Rizqi, Hamdan Dwi Robiatuz Zuniar Rohmah, Alya Awinatul Sabilla, Wardatus Salsabila, Finna Ananda Sigmatresna Wasatya Silvia Abdi Pratama Siti Mamluatus Sa'adah Sotya Rawiningtyas Sri Banun Titi Istiqomah Sri Fatmawati SRI FATMAWATI Sri Fatmawati Sri Fatmawati Sri Fatmawati Surya Rosa Putra Surya Rosa Putra, Surya Rosa Suryo Wiyono Syafri Izzat Abidiy Taslim Ersam Tatas, Tatas Taufiq Rinda Alkas Triono Bagus Saputro, Triono Bagus Weni Sri Ekowati Widhayanti, Nur Ari Widiya Nur Safitri Wilujeng, Susi Agustina Wiwit Denny Fitriana Yediya, Hanna Yulianengtias, Andira Yulinar Dwi Nur Azizah Yuliya Andriani Nanggewa Yunita Alfiyati Firdausa Yusuf Syahril Alam Zahra Salsabila Zahra, Ema Azzah Aulia Zakiyyah Yasmin Salsabila

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