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Potensi Antiinflamasi Ekstrak dan Fraksi n-Heksana Daun Galam (Melaleuca cajuputi) Asal Kalimantan Selatan Sutomo, Sutomo; Arnida, Arnida; Amalina, Amalina; Fadlilaturrahmah, Fadlilaturrahmah; Fitriana, Mia
BORNEO JOURNAL OF PHARMASCIENTECH Vol 9 No 2 (2025): Borneo Journal Of Pharmascientech
Publisher : Universitas Borneo Lestari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59053/bjp.v9i2.754

Abstract

South Kalimantan has a unique topography that includes tidal areas and swamps, and peatlands. One of the natural resources that grows well in this area is the galam tree (Melaleuca cajuputi). Traditionally, this plant has been used to treat various inflammatory conditions, such as intestinal inflammation, sore throat, itchy skin, and rheumatism. M. cajuputi contains flavonoid compounds that are believed to have anti-inflammatory properties. This study aimed to determine the anti-inflammatory activity of ethanol extracts and n-hexane fractions of M. cajuputi leaves from South Kalimantan using a protein denaturation inhibition method with IC50 values as the parameter. Sodium diclofenac was used as the positive control in this study. The results of the anti-inflammatory activity test showed that the IC50 values for sodium diclofenac, ethanol extract, and n-hexane fraction of M. cajuputi leaves were 14.948; 51.577; and 61.700 ppm, respectively. Statistical analysis revealed a significant difference between the IC50 value of sodium diclofenac and those of the ethanol extract and n-hexane fraction. Based on the study conducted, it can be concluded that the ethanol extract and n-hexane fraction have anti-inflammatory activity, although their potency is lower than that of sodium diclofenac
PENENTUAN NILAI SUN PROTECTION FACTOR (SPF) DAN AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL KULIT BATANG BANGKAL (Nauclea subdita) SECARA IN VITRO Rahmawanty, Dina; Maulina, Rizka; Fadlilaturrahmah, Fadlilaturrahmah
Media Farmasi: Jurnal Ilmu Farmasi Vol 14, No 2 : September 2017
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/mf.v14i2.11238

Abstract

Kulit batang bangkal (Nauclea subdita) telah lama digunakan sebagai kosmetika tradisional di Kalimantan Selatan. Kulit batang bangkal (KBB) diduga mengandung senyawa tabir surya dan antioksidan yang tinggi, seperti senyawa golongan flavonoid dan tanin. Penelitian ini bertujuan untuk mengetahui potensi tabir surya dan aktivitas antioksidan ekstrak etanol KBB. Penentuan nilai sun protecting factor (SPF) ekstrak etanol KBB diuji secara in vitro menggunakan spektrofotometer UV-Vis pada panjang gelombang 290-320 nm. Hasil penelitian menunjukkan bahwa nilai SPF ekstrak etanol KBB dengan konsentrasi 250 ppm sebesar 10, pada konsentrasi 500 ppm, nilai SPF-nya = 15, dan pada konsentrasi 1000 ppm, nilai SPF sebesar 29. Aktivitas antioksidan ekstrak etanol kulit batang bangkal diuji secara in vitro menggunakan metode penangkapan radikal DPPH (2,2-diphenyl-1-picrylhidrazyl) dengan Spektrofotometer UV-Vis pada panjang gelombang 521 nm. Nilai IC50 ekstrak etanol KBB sebesar 84,850 ppm. Berdasarkan nilai SPF dan nilai IC50 tersebut dengan demikian ekstrak etanol KBB memiliki kemampuan sebagai antioksidan dan tabir surya. 
Pemberdayaan Pengrajin Lulur Timung dalam Akselerasi Kualitas Home SPA Khas Suku Banjar Fitriana, Mia; Anwar, Khoerul; Fadlilaturrahmah, Fadlilaturrahmah
J-ABDIPAMAS (Jurnal Pengabdian Kepada Masyarakat) Vol. 8 No. 1 (2024): April 2024
Publisher : IKIP PGRI Bojonegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30734/j-abdipamas.v8i1.2297

Abstract

The traditions of balulur and batimung are still carried out today. One of the production places for cucumber scrub in the city of Banjarmasin is Ipah Zein MSME. The cucumber scrub product by Ipah Zein MSME is a concoction that has been passed down from generation to generation and has been producing cucumber scrub for three generations. During these three generations, the making of scrub and timung was still done traditionally. The quality of this product needs to be improved so that it can still be marketed. The implementation of this service aims to improve the quality dimensions of Ipah Zein MSME timung scrub products, both product quality and packaging quality. Activities will be carried out from April to December 2021 at the Ipah Zein timung scrub production house. The method used begins with preparation, then implementation of activities and evaluation of activities. Activities that have been carried out include modernizing equipment in the production process, assisting production according to Good Cosmetic Manufacturing Practices, mechanizing packaging equipment and improving traditional cosmetic packaging designs. The results show that the service activities have been carried out well, where there has been an increase in the quality of Ipah Zein MSME timung scrub products, both product quality and packaging quality. Service partners have been equipped with knowledge about Good Cosmetic Manufacturing Practices, given equipment that supports this and the product has a more attractive design and is more acceptable to the marketKeywords: scrubs, timung, quality Tradisi balulur dan batimung masih dilakukan sampai saat ini. Salah satu tempat produksi lulur timung di kota Banjarmasin adalah UMKM Ipah Zein. Produk lulur timung oleh UMKM Ipah Zein adalah ramuan warisan turun menurun dan sudah memproduksi lulur timung selama tiga generasi. Selama tiga generasi tersebut, pembuatan lulur dan timung masih dikerjakan secara tradisional. Produk ini perlu ditingkatkan kualitasnya agar tetap dapat dipasarkan. Pelaksanaan pengabdian ini bertujuan untuk meningkatkan dimensi kualitas produk lulur timung UMKM Ipah Zein, baik mutu produk maupun mutu kemasan. Kegiatan dilakukan pada bulan April sampai Desember tahun 2021 di rumah produksi lulur timung Ipah Zein. Metode yang dilakukan diawali dengan persiapan kemudian pelaksanaan kegiatan dan evaluasi kegiatan. Kegiatan yang telah terlaksana meliputi modernisasi peralatan pada proses produksi, pendampingan produksi sesuai Cara Pembuatan Kosmetik yang Baik (CPKB), mekanisasi alat pengemas dan peningkatan desain kemasan kosmetika tradisional. Hasil menunjukkan bahwa kegiatan pengabdian telah terlaksana dengan baik dimana terjadi peningkatan kualitas produk lulur timung UMKM Ipah Zein, baik mutu produk maupun mutu kemasan. Mitra pengabdian telah dibekali pengetahuan mengenai CPKB, diberikan peralatan yang menunjang hal tersebut dan produk telah memiliki desain yang lebih menarik dan lebih dapat diterima pasar.Kata Kunci: lulur, timung, kualitas 
The Antityrosinase Activity of Mitragyna speciosa Korth Leaf Ethanolic Extract Normaidah, Normaidah; Rahmah, Maslia; Izma, Hayatun; Fadlilaturrahmah, Fadlilaturrahmah; Sari, Anna Khumaira
Jurnal Ilmiah Kesehatan (JIKA) Vol. 6 No. 1 (2024): Volume 6 Nomor 1 April 2024
Publisher : Sarana Ilmu Indonesia (salnesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36590/jika.v6i1.761

Abstract

The kratom plant (Mitragyna speciosa Korth) is a the plant that is traditionally used to soften the skin. Kratom leaves have very strong antioxidant activity so they are predicted also to have anti-tyrosinase activity. The potential inhibition of tyrosinase enzyme activity is thought to come from the content of flavonoid compounds, polyphenols, steroids, tannins, and quinones. This research aims to determine the tyrosinase inhibitory activity of ethanol extract of M. speciosa leaves. The inhibitory activity of the tyrosinase enzyme using L-DOPA as substrate and kojic acid as positive control. Antityrosinase testing was carried out by in vitro method using UV-vis spectrophotometer through determination of IC50 value. The results of the antityrosinase activity test of ethanol extract of M. speciosa leaves showed an IC50 value of 2117.708 ppm. The ethanol extract of M. speciosa leaves is scientifically proven to have antityrosinase activity with a weak category.
Edukasi Penerapan Teknologi Fermentasi melalui Pembuatan Kefir dan Yogurt Khairunnisa, Amalia; Pratika Viogenta; Nani Kartinah; Arnida Arnida; Sutomo Sutomo; Fadlilaturrahmah Fadlilaturrahmah
Jurnal Nusantara Berbakti Vol. 2 No. 4 (2024): Oktober : Jurnal Nusantara Berbakti
Publisher : Universitas Kristen Indonesia Toraja

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59024/jnb.v2i3.411

Abstract

Fermentation technology can increase added value to agricultural, food and other products. Through fermentation, raw materials can be converted into products with higher selling value, such as cheese, yogurt, tempeh, wine, beer, and so on. Many fermented products are beneficial for health, for example kefir and yogurt which contain good probiotics. for digestion. Kefir is a type of fermented milk whose manufacture uses various microorganisms to produce lactic acid products with by-products such as ethanol, free fatty acids and acetaldehyde. Kefir is made by adding yeast which contains many microorganisms from the lactic acid bacteria and yeast groups, while yogurt is fermented milk with lactic acid bacteria (LAB).  This service activity aims to increase people' knowledge and skills regarding the application of fermentation technology, starting from the meaning of fermentation, fermentation methods to high-value products produced from the fermentation process. In this activity, socialization activities were carried out regarding the development of fermentation technology, especially kefir and cow's milk yogurt, as well as practices on how to make it among the residents of Balitra Jaya Permai Loktabat Utara. The results show an increase in residents' knowledge and skills regarding fermentation through pretest and posttest activities as well as practical activities.
Isolasi Dan Identifikasi Senyawa Antioksidan Dari Buah Kasturi (Mangifera casturi Kosterm.) Sutomo Sutomo; Hadi Azhari; Arnida Arnida; Fadlilaturrahmah Fadlilaturrahmah; Rahmat Yunus
Journal of Pharmascience Vol. 4 No. 2 (2017): Jurnal Pharmascience
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v4i2.5778

Abstract

ABSTRAK Antioksidan berfungsi untuk menetralisir radikal bebas, sehingga dapat mencegah dan melindungi terjadinya kerusakan tubuh yang dapat menyebabkan timbulnya penyakit degeneratif. Salah satu tanaman endemik Kalimantan Selatan yang teridentifikasi mengandung antioksidan alami yaitu kasturi (Mangifera casturi Kosterm.). Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi senyawa antioksidan dari buah M. casturi. Ekstraksi dilakukan dengan metode maserasi. Pemisahan dan pemurnian dilakukan dengan KLT , KCV, dan kromatografi kolom gravitasi. Identifikasi senyawa dengan spektrofotometri UV-Vis dan FTIR serta uji kualitatif antioksidan dan aktifitas kuantitatif antioksidan isolat. Ekstraksi 2500 gram serbuk buah M. casturi dengan metanol diperoleh 840,69 gram ekstrak kental berwarna coklat. Fraksinasi 60 gram ekstrak metanol dengan fraksi etil asetat menghasilkan 8,3 gram ekstrak kering. Fraksi etil asetat difraksinasi kembali menggunakan KCV dengan eluen n-heksana : etil asetat (9:1; 8:2; 7:3; 6:4; 5:5; 4:6; 3:7) v/v, etil asetat : metanol (5:5 v/v), 100% metanol diperoleh fraksi A, B, C, D, E, F, G, dan H. Fraksi H dipilih untuk di isolasi menggunakan kromatografi kolom gravitasi dengan eluen n-heksana : etil asetat : metanol (5:3:0,5; 5:4:1; 5:4:2; 5:6:2; 5:6:2,5) v/v dan 100% metanol. Uji kualitatif KLT menunjukkan isolat H-7 mengandung senyawa antioksidan dengan pereaksi DPPH. Aktivitas antioksidan isolat H-7 memiliki nilai IC50¬ sebesar 4,61±0,57 ppm. Analisis isolat H-7 dengan UV-Vis menghasilkan puncak pada λ 274,8 nm (pita I) dan λ 218 nm (Pita II). Analisis FTIR menunjukkan gugus fungsi dari isolat H-7 yaitu -OH, C-H aromatik, C-H alifatik, C=O, C=C aromatik, dan C-O. Kata Kunci : Antioksidan, buah M. casturi, DPPH, fraksi etil asetat, Isolasi
Isolasi Senyawa Antioksidan Fraksi Etil Asetat Daun Bilaran Tapah (Argyreia nervosa) Asal Rantau Kalimantan Selatan Sutomo Sutomo; Arnida Arnida; Nurmalita Sari; Fadlilaturrahmah Fadlilaturrahmah
Journal of Pharmascience Vol. 5 No. 1 (2018): Jurnal Pharmascience
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v5i1.5785

Abstract

ABSTRACT South Kalimantan is rich with medicinal herbs, one of it is Argyreia nervosa. Ethanol extract of A. nervosa leaves has potential as antioxidant with IC50 value of 9,46 ppm. This study aims to isolate antioxidant compounds from ethyl acetate fraction of A. nervosa leaves. Extraction method used by maceration. Separation and purification of isolates was done by TLC, VLC, and CC. Qualitative and quantitative test of antioxidant activity using diphenyl picrylhydrazyl (DPPH) method. Identification of compounds uses UV-Vis and FTIR spectrophotometers. Argyreia nervosa leaf powder was extracted with 96% ethanol and obtained a yield of 16,916%. Rendemen of ethyl acetate fraction is 16,33%. The ethyl acetate fraction is fractionated again using VLC with eluen n-hexane:ethyl acetate (20:1, 15:1, 10:1,9:1, 8:2, 7:3, 6:4; 5:5; 4:6; 3:7) v/v, and methanol:ethyl acetate (1:4) v/v obtained 5 fraction. The fraction 4 is used for isolation using the gravity CC with eluen n-hexane:ethyl acetate (20:1, 15:1, 10:1, 9:1, 7:3, and 5:5) v/v. Qualitative TLC test showed isolates containing antioxidant compounds with DPPH reagent. Analysis with UV-Vis spectrophotometer yielded 344 nm and 299 nm λmax. FTIR analysis shows there are functional groups -OH, C=O, C-O, C=C (alkene and aromatic), and C-H. Keywords: Antioxidants, DPPH, ethyl acetate fraction, A. nervosa leaves, isolation
Aktivitas Antibakteri Ekstrak Metanol Bunga Teratai (Nymphaea pubescens Willd) Amalia Khairunnisa; Nashrul Wathan; Mia Fitriana; Fadlilaturrahmah Fadlilaturrahmah; Nisriyati Fiddina
Journal of Pharmascience Vol. 7 No. 2 (2020): Jurnal Pharmascience
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v7i2.8486

Abstract

Nymphaea pubescens Willd telah diketahui mempunyai efek antibakteri, terutama pada biji dan daunnya. Tetapi sampai saat ini bagian bunga dari tanaman tersebut belum dilakukan pengujian aktivitas antibakteri. Tujuan dari penelitian ini untuk melakukan skrining fitokimia, uji aktivitas antibakteri dan penentuan konsentrasi hambat minimum (KHM) dari ekstrak metanol bunga N. pubescens terhadap S. aureus dan E. coli. Proses ekstraksi bunga N. pubescens dimaserasi menggunakan pelarut metanol dengan perbandingan 1: 4 b/v. Metode pengujian yang digunakan ada dua yaitu metode difusi untuk pengujian aktivitas antibakteri dan metode dilusi untuk pengukuran konsentrasi hambat minimum (KHM). Hasil skrining fitokimia didapatkan bahwa ekstrak metanol bunga N. pubescens mengandung senyawa golongan fenolik, saponin dan flavonoid. Hasil pengujian aktivitas antibakteri menunjukkan bahwa ekstrak metanol bunga N.pubescens mampu menghambat S. aureus (diameter hambat 10 ± 0,29 mm) dan E.coli (diameter hambat 10,2 ± 0,50 mm). Konsentrasi hambat minimum dari ekstrak metanol bunga N. pubescens terhadap S.aureus sebesar 12,5% dan terhadap E.coli sebesar 25%. Dapat disimpulkan bahwa ekstrak metanol bunga N. pubescens memiliki aktivitas sebagai antibakteri. Nymphaea pubescens Willd has known to have antibacterial effects, especially on the seeds and leaves. However, until now the flower of the plant has not been tested for antibacterial activity. The purpose of this study was to perform phytochemical screening, antibacterial activity test and determine the minimum inhibitory concentration (MIC) of the methanol extract of N. pubescens flowers against S. aureus and E. coli. The process of extracting N. pubescens flowers is macerated using methanol as a solvent with a ratio of 1: 4 w / v. There are two test methods used, namely the diffusion method for testing antibacterial activity and the dilution method for measuring the minimum inhibitory concentration (MIC). The results of phytochemical screening showed that the methanol extract of N. pubescens flowers contained phenolic compounds, saponins, and flavonoids. The results of the antibacterial activity test showed that the methanol extract of N.pubescens flowers was able to inhibit S. aureus (inhibition diameter 10 ± 0.29 mm) and E. coli (inhibitory diameter 10.2 ± 0.50 mm). The minimum inhibitory concentration of the methanol extract of N. pubescens flowers against S.aureus was 12.5% and against E. coli was 25%. It can be concluded that the methanol extract of N. pubescens flowers has antibacterial activityKeywords: Nymphaea pubescens, lotus flower, methanol extract, antibacterial
Characteristics Study and Total Flavonoids Quantification of Kareho Leaves (Callicarpa longifolia Lam) Nashrul Wathan; Fadlilaturrahmah Fadlilaturrahmah; Saufy Arishandi; Akhmad Rezeki Firdaus
Journal of Pharmascience Vol. 8 No. 2 (2021): Jurnal Pharmascience
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v8i2.10682

Abstract

The Callicarpa longifolia Lam. known as ‘kareho’ is one of the plants used by several Dayak tribes for traditional medicine such as antiacne, swelling, wounds, diarrhea, diabetes, and lowering cholesterol levels. This study has obtained scientific data on the characteristics of dried leaves and determined the flavonoids levels of methanol extract of C. longifolia leaves. Characterization includes both qualitative and quantitative examination on the dried leaves of C. longifolia leaves, while the determination of total flavonoid content was determined by the aluminium chloride method and calculated as quercetin equivalent (QE). The results of characteristics observations obtained organoleptic in the form of powder, dark green, bitter taste and have a distinctive smell. Microscopic test of leaves had an actinositic stomata type, ethanol soluble extract content of 17.50 ± 0.10% and water soluble extract content of 16.53 ± 0.37%. Drying loss 8.16 ± 0.25%, total ash content of 5.52 ± 0.06%, and acid insoluble ash content of 0.07 ± 0.01%. The results of phytochemical screening confirmed the presence of saponins, flavonoids, steroids, tannins, glycosides, and phenols. TLC observasions using 254 nm UV light obtained 6 spots and 7 spots appearance on sulphuric acid. It has total flavonoid levels obtained at 7.995 ± 0.050% w/w QE.
Uji Aktivitas Antioksidan dan Antitirosinase Fraksi n-Butanol Daun Sungkai (Peronema canescens Jack.) Secara Kualitatif Menggunakan Kromatografi Lapis Tipis Fadlilaturrahmah Fadlilaturrahmah; Aditya Maulana Perdana Putra; Muhammad Ikhwan Rizki; Titin Nor
Journal of Pharmascience Vol. 8 No. 2 (2021): Jurnal Pharmascience
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v8i2.11160

Abstract

Kerusakan oksidatif dalam tubuh manusia dapat disebabkan oleh radikal bebas, sehingga menimbulkan berbagai permasalahan. Daun P. canescens memiliki kandungan senyawa fenolik, tanin, alkaloid, steroid, saponin dan flavonoid yang potensial sebagai antioksidan. Tujuan penelitian ini yaitu untuk melakukan uji aktivitas antioksidan dan antitirosinase fraksi n-butanol daun sungkai secara kualittaif menggunakan kromatografi lapis tipis. Metode yang digunakan yaitu preparasi sampel dengan pembuatan simplisia yang kemudian diekstraksi secara maserasi menggunakan etanol 96%. Hasil ekstrak kental kemudian difraksinasi bertingkat dengan pelarut n-heksan, etil asetat, dan n-butanol. Selanjutnya dilakukan skrining fitokimia, uji aktivitas antioksidan, dan antitirosinase secara kualitatif menggunakan kromatografi lapis tipis. Hasil rendemen ekstrak etanol daun P. canescens yaitu sebesar 7,28% sedangkan rendemen fraksi yaitu 24,8%. Skrining fitokimia ekstrak etanol P. canescens mengandung alkaloid, flavonoid, terpenoid, steroid, tanin, saponin, dan fenol. Kesimpulan yang didapatkan dari penelitian ini yaitu fraksi n-butanol daun P. canscens berpotensi memiliki aktivitas antioksidan yang ditandai adanya noda kuning pucat setelah penyemprotan dengan reagen DPPH, serta berpotensi memiliki aktivitas antitirosinase secara kualitatif yang ditandai dengan adanya bercak putih pada plat KLT. Kata Kunci: DPPH, L-DOPA, Asam askorbat, Enzim  Oxidative damage in the human body can be caused by free radicals, causing various problems. P. canescens leaves contain phenolic compounds, tannins, alkaloids, steroids, saponins and flavonoids that have potential as antioxidants. The purpose of this study was to test the antioxidant and antityrosinase activity of the n-butanol fraction of sungkai leaves qualitatively using thin layer chromatography. The method used is sample preparation by making simplicia which is then extracted by maceration using 96% ethanol. The viscous extract was then fractionated with n-hexane, ethyl acetate, and n-butanol as solvents. Furthermore, phytochemical screening, antioxidant activity, and antityrosinase tests were carried out qualitatively using thin layer chromatography. The yield of P. canescens leaf ethanol extract was 7.28%, while the fraction yield was 24.8%. Phytochemical screening of P. canescens ethanol extract containing alkaloids, flavonoids, terpenoids, steroids, tannins, saponins, and phenols. The conclusion obtained from this study is that the n-butanol fraction of P. canscens leaves has the potential to have antioxidant activity which is indicated by a pale yellow stain after spraying with DPPH reagent, and has the potential to have qualitative antityrosinase activity which is indicated by the presence of white spots on the TLC plate.