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Journal : EnviroScienteae

REKLAMASI LAHAN KRITIS BEKAS PENAMBANGAN EMAS MENGGUNAKAN METODE BIOREMEDIASI DAN FITOREMEDIASI Liswara Neneng; Dewi Saraswati
EnviroScienteae Vol 15, No 2 (2019): EnviroScienteae Volume 15 Nomor 2, Agustus 2019
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.394 KB) | DOI: 10.20527/es.v15i2.6955

Abstract

Degraded land caused by gold mining activities in Central Kalimantan requires reclamation efforts, in order to restore of soil conditions, due to the damage of topsoil layer, lack of soil nutrients, and reduced of soil fertility. This research aim was to find an appropriate method of biological reclamation in ex gold mining area, by utilizing a combination of several types of microorganisms and mercury phytoremediator plants. The research is an experiment on a pilot scale at an ex gold mining site in Central Kalimantan. Bioremediation method has used a consortium of local isolates Klebsiella sp. and Pseudomonas sp., and the phytoremediation method was used Melastoma sp. and Cyperus sp. Dependent variables were soil mercury concentration and soil nutrients. The results showed the application of consortium microorganisms and mercury phytoremediator, were able to reduce soil mercury concentrations in averages 2.5 ppm, soil nutrient levels increased in averages 33%. These increased soil nutrients consist of potassium in averaged 73%, Mg in average 21%, Na in average 19.5%, and Fe in averaged 71%, when compared to controls. The application of consortium of microorganisms combined with mercury plant phytoremediator type Melastoma sp., able more to improve soil conditions in ex gold mining area compared to Cyperus sp.
POTENSI MIKROORGANISME SEBAGAI BIOFERTILIZER Lusia Widiastuti; Y. Sulistiyanto; Adi Jaya; Yusurum Jagau; Liswara Neneng
EnviroScienteae Vol 15, No 2 (2019): EnviroScienteae Volume 15 Nomor 2, Agustus 2019
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.37 KB) | DOI: 10.20527/es.v15i2.6957

Abstract

This research was carried out in the oil palm plantation of PT. MuliaSawitAgro Lestari (PT. MSAL) Gunung Mas Regency Central Kalimantan Province for 3 months starting in January 2019 until March 2019. The purpose of this study was to identify the potential of microorganisms as biofertilizers from palm oil mill effluent (PKS). This research is an exploratory study conducted in the Laboratory of the Faculty of Health Sciences of the Muhammadiyah University of Palangka Raya. This study used 3 (three) media used to identify the potential of microorganisms for biofertilizer from palm oil liquid waste by calculating the number of bacterial colonies namely Carboxy Methyl Cellulose (CMC), pykovskaya media and Yeast Extract Mannitol Agar (Yema) media. The results of the analysis also showed that PKS liquid waste contained potential microorganisms found were phosphate solvent bacteria, cellulite fungi and nitrogen-fixing bacteria with an average bacterial colony of 1,700,000 cells/mL. Potential microorganisms from the proportion obtained showed the ability of bacteria found in PKS liquid waste to degrade the oil. The first and second samples of bacterial forms identified were in the form of bacilli and were gram-positive bacteria, while the samples of the three bacteria were in the form of coccus and the bacteria were gram-negative bacteria.
PENGARUH TAHAPAN BIOREMEDIASI TERHADAP EFEKTIVITAS ELIMINASI MERKURI (Hg) DI MEDIA CAIR Liswara Neneng; Nawan Nawan; Asri Adelia
EnviroScienteae Vol 19, No 2 (2023): ENVIROSCIENTEAE VOLUME 19 NOMOR 2, MEI 2023
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/es.v19i2.14883

Abstract

Gold miners use mercury for the gold extraction process, and annually releases no less than 1,000 tons of hazardous materials into the environment. The mercury content in the Kahayan watershed reaches 2,996 - 4,687 g/l and this value exceeds the standard limit for mercury content in water. Mercury is highly toxic so that it causes negative impacts on the environment and public health. Bioremediation is one of solution to reduce mercury waste pollution. Mercury bioremediation using microorganisms and adsorbents requires appropriate process steps to be effective in eliminating mercury. This study aims to determine the effect of the bioremediation steps on the effectiveness of mercury (Hg) elimination in liquid media and to determine the most effective bioremediation steps for mercury (Hg) elimination in liquid media. This study used an experimental method with a non-factorial Completely Randomized Design consisting of treatments, namely control (K), treatment 1 (T1), treatment 2 (T2), treatment 3 (T3), treatment 4 (T4), with 5 (five) times repetition. Analysis the results of the bioremediation stage used AAS (Atomic Absorption Spectrophotometry). Data were analyzed using One-way Anova test, LSD test and effectiveness test. The results showed that the bioremediation stage had a significant effect on the effectiveness of mercury (Hg) elimination in liquid media, this was proven by the results of statistical tests carried out using the One-way Anova test and further tests where the different reduction data obtained from each treatment. The initial level mercury 10 ppm, after being given treatment decreased T1 to 1.25 ppm, T2 to 1.33 ppm, T3 to 1.06 ppm, and T4 to 1.24 ppm. The most effective bioremediation step for the elimination of mercury (Hg) in liquid media is the T3 stage starting from Mercury Waste→Microalgae→Bacteria→Red Clay→Activated Charcoal, with a mercury reduction effectiveness value of 89.4%.