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Journal : Bioscience

Analysis of Genetic Profiles of Heavy Metal Phytoremediator Plants From Gold Mining Areas Sih Winarti; Liswara Neneng; Yohanes Edy Gunawan; Chaidir Adam
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.732 KB) | DOI: 10.24036/0202041108305-0-00

Abstract

The resistance and survival ability of phytoremediator plants in a polluted environment is thought to be a form of genetic adaptation. This research aimed: (1) to identify the dominant plant species in ex-gold mining area; (2) to analyze the genetic profile of phytoremediator plants from ex-gold mining area; and (3) to compare the genetic profile of heavy metal phytoremediator plants from ex-gold mining area with the same plant species from non-mining area. The samples of this study were Cyperus sp., Lycopodium sp., and Melastoma sp. The research procedures carried out include sample collection, DNA isolation, DNA amplification with PCR, and DNA visualization with Electrophoresis. The results show that the dominant plant species of ex-gold mining area are Cyperus sp., Lycopodium sp., and Melastoma sp. The genetic profile analysis of dominant plant species of ex-gold mining area show that no DNA bands appeared from the target gene as the result of amplification using specific primers of Metallothionein gene. The result of RAPD analysis using OPA-04 universal primers show that at 500-750 bp there are differences in DNA bands that appeared between the samples. DNA bands that appeared in the genetic profile of phytoremediator plants is thought to be the representation of the gene that responsible for heavy metals tolerance.
Co-Authors Abriyanto Adam, Chaidir Adi Jaya Akhmadi Akhmadi Amalia Yulianti Anita Maulina Araina, Elga Ardianoor Ardianoor Arintiana Ghazella Asri Adelia Asri Adelia Asri Pujirahadju Bambang S. Lautt Bambang Supriono Lautt Basuki, Bejo Bejo Basuki Bejo Basuki Chaidir Adam Chuchita Desimaria Panjaitan Dewi Saraswati Dicky Satriya Pratama Ebrry Dwi Putra Ennike Gusti Rahmi Fachry Abda El Rahman Fahrina, Ririn Ferayani Ferayani Ferayani, Ferayani Frans Grovy Naibaho Frenklin Yuda Laba Gamaliel Gamaliel Gunawan, Yohanes E Gunawan, Yohanes Edy Hartanti, Rio Eka Desi Purwandari Herniati , Melly Indah Sari Dewi Indri Aprilia, Indri Kusuma Putri, Rahmadyah Lautt, Bambang S. Ludang, Yetrie Lusia Widiastuti Lusia Widyastuti Meitiana Miranda, Yula Muhammad Nuruddin Naibaho, Frans Nawan Nawan Nawan Nawan Nawan, Nawan Ngazizah, Febri Nur Oksal, Efriyana Panny, Priscilla Pujirahadju, Asri Rahmad Hidayat Rahmawati, Rahmawati Ria Anjalani Rio Eka D.P. Hartanti Ruben Tinting Sirenden Sari, Nopita Tamara Sih Winarti Simatupang, Tumpal Siti Sunariyati Srininta Br Perangin-angin Stevin Carolius Angga Sulmin Gumiri Sulmin Gumiri Susilawati Susilawati Susilawati Susilawati Susilawati, Susilawati Syarpin Syarpin Tanduh, Yusintha Titin Purnaningsih Tri Widayat Tri Widayat Tumpal Simatupang Usop, Aswin Usup, Hepryandi Luwyk Djanas Vivin Alvianita Wijaya, Nuriman Y. Sulistiyanto Yetrie Ludang Yohanes Edy Gunawan Yula M Yula M, Yula Yustinus Sulistiyanto Yusurum Jagau Yusurum Jagau Zakaria Zakaria Zimon Pereiz