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All Journal Journal of Islamic Pharmacy Pharmaceutical Journal of Indonesia Lumbung Farmasi : Jurnal Ilmu Kefarmasian Hubungan Tingkat Pengetahuan Petugas Pengelola Obat dengan Tingkat Ketersediaan Obat Di Puskesmas Kota Malang PARAHITA : Jurnal Pengabdian kepada Masyarakat Jurnal Riset Farmasi Jurnal Ilmiah Sains JPMP Medical Sains : Jurnal Ilmiah Kefarmasian Jurnal Sains dan Kesehatan
Astrid Kusuma Putri
Program Studi Farmasi, Fakultas Farmasi, Universitas Hang Tuah, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Physics Public Health Social Sciences

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Telaah Metodologis Uji DPPH pada Metabolit Sekunder Bahan Alam Astrid Kusuma Putri; Asti Rahayu; Rina Andayani; Ersanda Nurma Praditapuspa; Yuyun Nailufa; Etik Wahyuningsih
Jurnal Riset Farmasi Volume 6, No. 1, Juli 2026, Jurnal Riset Farmasi (JRF)
Publisher : UPT Publikasi Ilmiah Unisba

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/jrf.v6i1.9972

Abstract

Abstract. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay is one of the most widely used spectrophotometric methods for evaluating the antioxidant activity of natural product extracts because it is simple, rapid, and cost-effective. However, DPPH assay results are influenced by methodological variations, including solvent selection, initial DPPH concentration and absorbance, reagent-to-sample volume ratio, incubation time, measurement wavelength, and data processing, which may reduce result comparability when experimental conditions are inadequately reported. This study aimed to identify methodological variations in the DPPH assay, evaluate their potential influence on antioxidant activity results, and formulate recommendations for the minimum methodological parameters that should be reported. A structured narrative review following the PRISMA framework was conducted using publications from the Scopus, Google Scholar, and SINTA databases (2004–2025). Sixteen eligible articles were analyzed using a descriptive-comparative approach based on Molyneux's conceptual framework. The review identified methodological heterogeneity and inconsistent reporting as major factors affecting the interpretation and comparability of DPPH assay results. The resulting recommendations aim to enhance methodological transparency, reproducibility, and comparability in natural product pharmaceutical research. Abstrak. Uji 2,2-difenil-1-pikrilhidrazil (DPPH) merupakan salah satu metode spektrofotometri yang banyak digunakan untuk mengevaluasi aktivitas antioksidan ekstrak bahan alam karena prosedurnya sederhana, cepat, dan ekonomis. Namun, hasil uji aktivitas antioksidan dipengaruhi oleh variasi parameter metodologis, seperti jenis pelarut, konsentrasi dan absorbansi awal DPPH, rasio volume reagen dan sampel, waktu inkubasi, panjang gelombang pengukuran, serta pengolahan data, sehingga berpotensi menurunkan keterbandingan hasil apabila kondisi eksperimental tidak dilaporkan secara memadai. Artikel ini bertujuan mengidentifikasi variasi parameter metodologis pada uji DPPH, mengevaluasi potensi pengaruhnya terhadap hasil pengujian, serta merumuskan rekomendasi parameter minimum yang perlu dilaporkan. Penelitian disusun sebagai structured narrative review dengan pendekatan PRISMA terhadap publikasi dari basis data Scopus, Google Scholar, dan SINTA tahun 2004–2025. Sebanyak 16 artikel memenuhi kriteria inklusi dan dianalisis secara deskriptif-komparatif menggunakan kerangka konseptual Molyneux. Hasil telaah menunjukkan bahwa heterogenitas kondisi eksperimen dan pelaporan parameter reaksi memengaruhi interpretasi dan keterbandingan hasil. Berdasarkan sintesis literatur, artikel ini merumuskan rekomendasi parameter metodologis minimum untuk meningkatkan transparansi metodologis, reprodusibilitas, dan keterbandingan hasil uji DPPH dalam penelitian farmasi bahan alam.
Pengaruh Pelarut Metanol dan Etanol Terhadap Kadar Flavonoid Total Ekstrak Kulit Pohon Waru (Hibiscus tiliaceus L.) Astrid Kusuma Putri; Lunardhi Susanto; Deva Oktavy American Iswardjono; Asti Rahayu; Lindawati Setyaningrum
Lumbung Farmasi: Jurnal Ilmu Kefarmasian Vol 5, No 2 (2024): Juli
Publisher : UNIVERSITAS MUHAMMADIYAH MATARAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/lf.v5i2.17465

Abstract

Sea hibiscus (Hibiscus tiliaceus L.) is one of the plants in Indonesia that is empirically used as a traditional medicine. The study aims to determine the total flavonoid content of methanol and ethanol extracts from the sea hibiscus tree bark using UV-Vis spectrophotometry. Phytochemical screening of flavonoids is done using concentrated magnesium and hydrochloric acid (HCl). Total flavonoid levels are determined using the AlCl3 method specified in the QE (Quercetin equivalent). The phase of the research method includes preparation of methanol extract and ethanol extracts, phytochemical screening of flavonoid content, quantitative analysis of the flavonoid content (determination of the maximum wavelength (λmax) of quercetin, standard curve preparations of quercetin, and determination of flavonoid contents in methanol extract and ethanol extract), data management and analysis. Phytochemical screening showed methanol extracts and ethanol extract of the Sea Hibiscus tree bark showed positive contain flavonoids. Quantitative tests using UV-Vis spectrophotometry measured at 441 nm wavelengths showed that the concentration of flavonoid compounds in the Sea Hibiscus tree bark methanol extract was 0.109 %b/b, and the concentrations of flavonoids in the ethanol extracts were 0.096 %b/b.
Co-Authors Andayani, Rina Asti Rahayu Asti Rahayu Deva Oktavy American Iswardjono Dewi Perwito Sari Ersanda Nurma Praditapuspa Etik Wahyuningsih Faqihuddin Faqihuddin Fithri Farchah Frischah Sari Hardani, Prisma Trida Hardiyono Hardiyono Izazi, Farizah Kresnamurti, Angelica Lindawati Setyaningrum Liza Yudistira Yusan Lunardhi Susanto Mochamad Yuwono Nadya Ambarwati Nailufa, Yuyun Nani Wijayanti Dyah Nurrahman Praditapuspa, Ersanda Nurma Purbosari, Ira Rachma Nurhayati Rakhma, Dita Nurlita Rina Andayani Rizky Anugrah Dirgantara Putra Wahyudi Wahyudi Yanu Andhiarto Yuwono, Mochamad Yuyun Nailufa