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All Journal Jurnal Ilmu Pertanian Indonesia Biospecies Jurnal Agroindustri Jurnal Penelitian Sains AL-HAYAT: Journal of Biology and Applied Biology Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) BIO-EDU: Jurnal Pendidikan Biologi Jurnal Riset Biologi dan Aplikasinya Jurnal Ilmiah Biologi UMA (JIBIOMA) Jurnal Jaring SainTek BIOEDUPAT: Pattimura Journal of Biology and Learning NEAR: Jurnal Pengabdian kepada Masyarakat AMMA : Jurnal Pengabdian Masyarakat Jurnal Pengabdian Masyarakat Panacea
Kusuma Handayani, Kusuma
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ISOLASI, IDENTIFIKASI DAN PEMBUATAN INOKULUM FUNGI SEBAGAI PENDEGRADASI LIMBAH PADAT TANAMAN TEBU (Saccharum officinarum L.) Supriyanti, Arni; Sumardi; Yusnaini, Sri; Agustrina, Rochmah; Handayani, Kusuma; Musa; Pramana, Yanuar Sigit
Jurnal Agroindustri Vol. 15 No. 1 (2025): May 2025
Publisher : BPFP Faperta UNIB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jagroindustri.15.1.97-111

Abstract

Penelitian ini bertujuan untuk memperoleh isolat fungi yang mampu dalam mendegradasi bagas tebu. Mengetahui pengaruh substrat jagung dan onggok terhadap pertumbuhan isolat fungi. Mengetahui bobot dan ratio C/N bagas tebu yang diberi inokulum fungi yang diambil dari media jagung dan onggok. Penelitian dilakukan di Laboratorium Mikrobiologi Universitas Lampung. Bulan November 2023 s.d April 2024.  Penelitian dilakukan secara faktorial dalam Rancangan Acak Lengkap (RAL).  Faktor pertama jenis substrat; jagung dan onggok.  Faktor kedua adalah isolat yang digunakan yaitu isolate Trichoderma sp komersial isolat fungi diduga Trichoderma sp., isolat diduga fungi Coprinus sp., isolat diduga fungi Neurospora sp., dengan pengulangan 3 kali.  Parameter yang diamati adalah jumlah inokulum, bobot degradasi bagas tebu dan rasio C/N.  Data heterogen dan tidak normal, dilakukan uji Kruskal-Wallis, dilanjutkan dengan uji lanjut Mann-Whitney pada taraf 5%. Uji Kruskal-Wallis tidak menunjukkan adanya beda nyata akibat perlakuan.  Hasil yang diperoleh adalah isolat yang mampu mendegradasi bagas tebu, yaitu diperolehnya sembilan isolat, tujuh diantaranya termasuk genus Trichoderma, satu genus Coprinus dan satu genus Neurospora. ubstrat jagung dan onggok dapat digunakan sebagai media alternatif dalam pembuatan inokulum fungi, dimana keduanya tidak menunjukkan perbedaan yang signifikan terhadap respon jumlah inokulum.  Penurunan bobot serta rasio C/N selama 28 hari dengan pemberian inokulum fungi tidak menunjukkan hasil yang baik.
Identifikasi Bakteri Pelarut Kalium Indigenus Asal Perkebunan Nanas Handayani, Kusuma; Mirawanty Amin
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 1 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.1.47

Abstract

The use of relatively high doses of inorganic fertilizers to increase yields in plantation-scale pineapple (Ananas comosus) cultivation can lead to new problems, such as being toxic to plants, polluting the environment, and increasing production costs. The utilization of rhizosphere microbes, such as potassium solubilizing bacteria (KSB) is expected can reduce the dose of inorganic K fertilizer application because it can dissolve potassium-fixed Al and Fe soil so that it is available to plants. This study aims to assess and characterize the potential of BPK in pineapple plantation areas. KSB evaluation was carried out based on the Potassium Solubility Index (IKK), besides that, it also carried out the characterization of bacteria that have the potential as CPC isolates. CPC was isolated from planting blocks with low and high production levels in the vegetative (V) and generative (G) phases with 5 replications. As many as 20 isolates from the CPC population on pineapple plantations were successfully isolated in this study. The lowest K solubility index of 0.6 was obtained in isolate RV1 and the highest, namely 1.2 in isolate RG1. Among these isolates, RG1 is Gram-positive and grows optimally at pH 5, temperature 30°C. Based on the results of the phylogenetic analysis, it is known that this potassium solubilizing isolate has the same level of similarity with the bacterium Bacillus subtilis. Keywords: Bacillus, indigenous, potassium, solubilizing index, temperature
Dinamika Populasi Azotobacter pada Tanah Ultisol Pertanaman Nanas: Pengaruh Kedalaman Bajak dan Jenis Kompos Sumardi, Sumardi; Ramadhani, Winih Sekaringtyas; Handayani, Kusuma; Triyono, Sugeng; Dermiyati, Dermiyati; Yusnaini, Sri; Pamela, Ahmad Bintang
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 7, No 2 (2025): November 2025
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v7i2.5702

Abstract

The decline in pineapple production in Lampung Tengah regency occurred in 2023, where the decline reached 16.4% of the pineapple production in 2022 (8.6 million quintals). The decline in pineapple production came from PT Great Giant Pineapple (PT GGP), with the problem of accumulation of pineapple plant litter polymers on ultisol land. The solution that emerged was the adding of biochar, vermicompost and Azotobacter (LOB), with various plowing depth. The aim of this research was to determine the dynamics of Azotobacter population (log CFU g-1) in the ultisol soil of PT GGP’s pineapple plantations which were influenced by plowing depth, compost mixture and interaction between them. The supporting variables were the temperature (0C), water content (%), pH and soil organic carbon (%). Data analysis included ANOVA (α 5%), Tukey’s HSD Test (α 5%) and Pearson R Test (α 1%). The results showed that the Azotobacter population in pre-planting (PT) samples differed significantly between plowing depth, compost mixture and the interaction of the two factors. Deep plow treatment (± 40 cm) with finely chopped pineapple litter (L1) and application of premium GGP’s compost + LOB (P3) was the best treatment, with 4,96 log CFU g-1 Azotobacter population. Azotobacter population correlated not significantly with any supporting variables.
Biological Control Agent of Spodoptera frugiperda Using Bacillus thuringiensis Bacteria Indriyani, Indriyani; Handayani, Kusuma; Firmansyah, Alvian; Pratami, Gina Dania
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i2.26475

Abstract

The main obstacle in the development of food crop production and horticulture is the attack of S. frugiperda larvae. Pest control using chemical insecticides has a long-term negative impact. The biological control of Spodoptera frugiperda larvae is accomplished by utilizing B. thuringiensis bacteria. This study aims to determine the best B. thuringiensis isolate for killing S. frugiperda larvae, to identify the most effective concentration of B. thuringiensis suspension, and to ascertain the mortality rate of S. frugiperda larvae. The design of this study was a randomized block design (RBD) with two factors. The first factor was the type of B. thuringiensis isolate, consisting of four isolates. Larval mortality data were analyzed using Analysis of Variance (ANOVA) and further tested with the Duncan test. The results obtained showed the highest average mortality of S. frugiperda larvae using isolates Bt3BP14 and Bt4TSR6. These two isolates had the highest average mortality on the third day. Bt3BP14 and Bt4TSR6 isolates have high potential in controlling S. frugiperda larvae, with an average mortality proportion of 86,67% and 66,67% observed over three days. The best doses of B. thuringiensis suspension for killing S. frugiperda larvae were 15 ml and 20 ml. It can be concluded that B. thuringiensis isolates can be used as natural biological control agents against S. frugiperda larvae that attack food crops and horticulture.
Aquatic Insect Diversity as Bioindicators of Water Quality in Organic Inorganic Rice Fields, Pringsewu, Lampung: Keanekaragaman Serangga Air sebagai Indikator Kualitas Air di Sawah Organik Anorganik di Pringsewu Lampung Pramudita, Rima; Dania Pratami, Gina; Meidaliyantisyah; Handayani, Kusuma
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 2 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j-bekh.v12i2.424

Abstract

The diversity of aquatic insects serves as an important indicator for assessing the quality of agricultural ecosystems, particularly in organic and conventional (non-organic) rice fields. Organic farming, which relies on natural inputs, tends to support greater biodiversity, whereas conventional farming may reduce it due to the use of chemical substances.   This   study   aimed   to   examine   the diversity, dominance, evenness, and environmental factors influencing the presence of aquatic insects in two types of agricultural systems in Tulung Agung Village, Lampung. The results showed that the organic   rice   fields   had   higher   aquatic   insect diversity, with a diversity index (H') of 2.3386, a dominance index (D) of 0.058, and an evenness index (E) of 0.9411. In contrast, the conventional rice fields showed a diversity index (H') of 0.9743, a dominance index (D) of 0.5188, and an evenness index (E) of 0.3312. Environmental factors had no significant effect on aquatic insects in the organic fields, but showed a strong correlation in the conventional fields, with a correlation value (r) of 0.905 and a significance value (p) of 0.035. These findings highlight that organic farming systems are better able to support a more stable aquatic ecosystem balance.
Mortality and Morphological Changes in Aedes aegypti Larvae by Bacillus thuringiensis and Serratia marcescens: Mortalitas dan Perubahan Morfologi Larva Aedes aegypti oleh Bacillus thuringiensis dan Serratia marcescens Yulia Sari, Umi; Rosa, Emantis; Handayani, Kusuma; Busman, Hendri
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 2 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The use of biolarvicides is an alternative biological agent to replace synthetic insecticides, namely entomopathogenic bacteria. The purpose of this study was to determine the potential of Bacillus thuringiensis and Serratia marcescens extracts on mortality and morphological changes in Aedes aegypti larvae. This study was a 2-factor experimental study using a Randomized Block Design (RBD). The first factor, the type of entomopathogenic bacteria, namely B. thuringiensis and S. marcescens. The second factor, the concentration of bacterial extracts (60 mL, 70 mL, 80 mL), negative control (sterile distilled water). Mortality data were processed using ANOVA at the 5% level. The results showed that the administration of B. thuringiensis and S. marcescens extracts caused mortality and morphological changes in Ae. aegypti larvae. B. thuringiensis at concentrations of 60 mL, 70 mL, and 80 mL caused 100% larval mortality, while S. marcescens at concentrations of 60 mL resulted in 70%, 53%, and 50% at concentrations of 70 mL and 80 mL, respectively. Morphological changes in larvae were indicated by changes in color, texture, and damage to the digestive system. It was concluded that B. thuringiensis has the highest potential as an entomopathogenic agent compared to S. marcescens in causing mortality and morphological changes in Ae. aegypti larvae.
Antibacterial Activity Test of Methanol Extract of Gracilaria sp. against Escherichia coli Using Disc Diffusion Method: Uji Aktivitas Antibakteri Ekstrak Metanol Gracilaria sp. terhadap Escherichia coli Dengan Metode Difusi Cakram Ajeng Pawesti, Ammanda Sadiva; Handayani, Kusuma; Setyaningrum, Endah; Busman, Hendri
BIO-EDU: Jurnal Pendidikan Biologi Vol. 10 No. 3 (2025): BIO-EDU: Jurnal Pendidikan Biologi - December 2025
Publisher : Jurusan Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jbe.v10i3.9389

Abstract

The utilization of marine resources as an alternative source of natural antibacterial agents has been increasingly developed in recent years. Gracilaria sp., a type of red algae, is known to contain biologically active compounds with potential antibacterial properties, particularly against Escherichia coli. This study aims to determine the antibacterial potential of methanol extract from Gracilaria sp. against E. coli using an experimental method with a Completely Randomized Design (CRD). Data were analyzed using One-Way ANOVA followed by Tukey's test. The stock solution of the extract was prepared at a concentration of 100 mg/mL and tested at concentrations of 0.05%, 1%, 5%, and 10%, with ciprofloxacin as the positive control and sterile distilled water as the negative control. The experiment was conducted with three replications. The results showed that the methanol extract of Gracilaria sp. formed inhibition zones at the highest concentrations of 10% and 5%, with an average diameter of only 0.075 mm. Thus, the methanol extract of Gracilaria sp. exhibits weak antibacterial potential against Escherichia coli, as the inhibition zone formed was significantly smaller than that of the positive control, which reached 1.7 mm.
Co-Authors Abdullah Setiawan, Wawan Abdullah, Wawan Setiawan Ajeng Pawesti, Ammanda Sadiva Andi Setiawan Arifiyanto, Achmad Ayuandira, Niken Damayanti, Berliana Dania Pratami, Gina Dermiyati, Dermiyati Elly Lestari Rustiati Endah Setyaningrum, Endah Ernawiati, Eti Fadia, Yasmin Firmansyah, Alvian Gina Dania Pratami, Gina Dania Hendri Busman Indriyani Indriyani Indriyani John Hendri Kanedi, Mohamad Krisna Rendi Awalludin Mayuri, Nindya Sekar Meidaliyantisyah Mirawanty Amin Musa Nainggolan, Ribka Debora Nuning Nurcahyani Pamela, Ahmad Bintang Pramana, Yanuar Sigit Pramudita, Rima Pratiwi, Ratih - Prihadhi, Eka Primasari Pertiwi Putri, Meishy Handerlin Ramadhani, Winih Sekaringtyas Ratih, Cindy Lukyta Riandani Azzahra, Syifa Rochmah Agustrina Rosa, Emantis Rumidatul, Alfi Setiawan, Wawan A Sri Yusnaini Sugeng Triyono Sumardi Sumardi . Supriyanti, Arni Susanto, Gregorius Nugroho Tugiyono Tugiyono Tundjung Tripeni Handayani, Tundjung Tripeni WAWAN ABDULLAH SETIAWAN, WAWAN ABDULLAH Wulandari, Adelia saviera Yulia Sari, Umi