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Kusuma Handayani, Kusuma
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ISOLASI, IDENTIFIKASI DAN PEMBUATAN INOKULUM FUNGI SEBAGAI PENDEGRADASI LIMBAH PADAT TANAMAN TEBU (Saccharum officinarum L.) Supriyanti, Arni; Sumardi; Yusnaini, Sri; Agustrina, Rochmah; Handayani, Kusuma; Musa; Pramana, Yanuar Sigit
Jurnal Agroindustri Vol. 15 No. 1 (2025): May 2025
Publisher : BPFP Faperta UNIB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jagroindustri.15.1.97-111

Abstract

Penelitian ini bertujuan untuk memperoleh isolat fungi yang mampu dalam mendegradasi bagas tebu. Mengetahui pengaruh substrat jagung dan onggok terhadap pertumbuhan isolat fungi. Mengetahui bobot dan ratio C/N bagas tebu yang diberi inokulum fungi yang diambil dari media jagung dan onggok. Penelitian dilakukan di Laboratorium Mikrobiologi Universitas Lampung. Bulan November 2023 s.d April 2024.  Penelitian dilakukan secara faktorial dalam Rancangan Acak Lengkap (RAL).  Faktor pertama jenis substrat; jagung dan onggok.  Faktor kedua adalah isolat yang digunakan yaitu isolate Trichoderma sp komersial isolat fungi diduga Trichoderma sp., isolat diduga fungi Coprinus sp., isolat diduga fungi Neurospora sp., dengan pengulangan 3 kali.  Parameter yang diamati adalah jumlah inokulum, bobot degradasi bagas tebu dan rasio C/N.  Data heterogen dan tidak normal, dilakukan uji Kruskal-Wallis, dilanjutkan dengan uji lanjut Mann-Whitney pada taraf 5%. Uji Kruskal-Wallis tidak menunjukkan adanya beda nyata akibat perlakuan.  Hasil yang diperoleh adalah isolat yang mampu mendegradasi bagas tebu, yaitu diperolehnya sembilan isolat, tujuh diantaranya termasuk genus Trichoderma, satu genus Coprinus dan satu genus Neurospora. ubstrat jagung dan onggok dapat digunakan sebagai media alternatif dalam pembuatan inokulum fungi, dimana keduanya tidak menunjukkan perbedaan yang signifikan terhadap respon jumlah inokulum.  Penurunan bobot serta rasio C/N selama 28 hari dengan pemberian inokulum fungi tidak menunjukkan hasil yang baik.
Identifikasi Bakteri Pelarut Kalium Indigenus Asal Perkebunan Nanas Handayani, Kusuma; Mirawanty Amin
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 1 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.1.47

Abstract

The use of relatively high doses of inorganic fertilizers to increase yields in plantation-scale pineapple (Ananas comosus) cultivation can lead to new problems, such as being toxic to plants, polluting the environment, and increasing production costs. The utilization of rhizosphere microbes, such as potassium solubilizing bacteria (KSB) is expected can reduce the dose of inorganic K fertilizer application because it can dissolve potassium-fixed Al and Fe soil so that it is available to plants. This study aims to assess and characterize the potential of BPK in pineapple plantation areas. KSB evaluation was carried out based on the Potassium Solubility Index (IKK), besides that, it also carried out the characterization of bacteria that have the potential as CPC isolates. CPC was isolated from planting blocks with low and high production levels in the vegetative (V) and generative (G) phases with 5 replications. As many as 20 isolates from the CPC population on pineapple plantations were successfully isolated in this study. The lowest K solubility index of 0.6 was obtained in isolate RV1 and the highest, namely 1.2 in isolate RG1. Among these isolates, RG1 is Gram-positive and grows optimally at pH 5, temperature 30°C. Based on the results of the phylogenetic analysis, it is known that this potassium solubilizing isolate has the same level of similarity with the bacterium Bacillus subtilis. Keywords: Bacillus, indigenous, potassium, solubilizing index, temperature
Dinamika Populasi Azotobacter pada Tanah Ultisol Pertanaman Nanas: Pengaruh Kedalaman Bajak dan Jenis Kompos Sumardi, Sumardi; Ramadhani, Winih Sekaringtyas; Handayani, Kusuma; Triyono, Sugeng; Dermiyati, Dermiyati; Yusnaini, Sri; Pamela, Ahmad Bintang
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 7, No 2 (2025): November 2025
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v7i2.5702

Abstract

The decline in pineapple production in Lampung Tengah regency occurred in 2023, where the decline reached 16.4% of the pineapple production in 2022 (8.6 million quintals). The decline in pineapple production came from PT Great Giant Pineapple (PT GGP), with the problem of accumulation of pineapple plant litter polymers on ultisol land. The solution that emerged was the adding of biochar, vermicompost and Azotobacter (LOB), with various plowing depth. The aim of this research was to determine the dynamics of Azotobacter population (log CFU g-1) in the ultisol soil of PT GGP’s pineapple plantations which were influenced by plowing depth, compost mixture and interaction between them. The supporting variables were the temperature (0C), water content (%), pH and soil organic carbon (%). Data analysis included ANOVA (α 5%), Tukey’s HSD Test (α 5%) and Pearson R Test (α 1%). The results showed that the Azotobacter population in pre-planting (PT) samples differed significantly between plowing depth, compost mixture and the interaction of the two factors. Deep plow treatment (± 40 cm) with finely chopped pineapple litter (L1) and application of premium GGP’s compost + LOB (P3) was the best treatment, with 4,96 log CFU g-1 Azotobacter population. Azotobacter population correlated not significantly with any supporting variables.
Co-Authors Abdullah Setiawan, Wawan Abdullah, Wawan Setiawan Andi Setiawan Arifiyanto, Achmad Ayuandira, Niken Damayanti, Berliana Dania Pratami, Gina Dermiyati, Dermiyati Elly Lestari Rustiati Ernawiati, Eti Fadia, Yasmin Indriyani John Hendri Kanedi, Mohamad Krisna Rendi Awalludin Mayuri, Nindya Sekar Mirawanty Amin Musa Nainggolan, Ribka Debora Nuning Nurcahyani Pamela, Ahmad Bintang Pramana, Yanuar Sigit Pratiwi, Ratih - Prihadhi, Eka Primasari Pertiwi Putri, Meishy Handerlin Ramadhani, Winih Sekaringtyas Ratih, Cindy Lukyta Riandani Azzahra, Syifa Rochmah Agustrina Rumidatul, Alfi Setiawan, Wawan A Sri Yusnaini Sugeng Triyono Sumardi Sumardi . Supriyanti, Arni Susanto, Gregorius Nugroho Tugiyono Tugiyono Tundjung Tripeni Handayani, Tundjung Tripeni WAWAN ABDULLAH SETIAWAN, WAWAN ABDULLAH Wulandari, Adelia saviera