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All Journal Jurnal Ilmu Pertanian Indonesia Biospecies Jurnal Agroindustri Jurnal Penelitian Sains AL-HAYAT: Journal of Biology and Applied Biology Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) BIO-EDU: Jurnal Pendidikan Biologi Jurnal Riset Biologi dan Aplikasinya Jurnal Ilmiah Biologi UMA (JIBIOMA) JURNAL BIOSHELL Jurnal Jaring SainTek BIOEDUPAT: Pattimura Journal of Biology and Learning Jurnal Pengabdian Masyarakat Panacea
Kusuma Handayani, Kusuma
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary

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Biological Control Agent of Spodoptera frugiperda Using Bacillus thuringiensis Bacteria Indriyani, Indriyani; Handayani, Kusuma; Firmansyah, Alvian; Pratami, Gina Dania
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 2 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i2.26475

Abstract

The main obstacle in the development of food crop production and horticulture is the attack of S. frugiperda larvae. Pest control using chemical insecticides has a long-term negative impact. The biological control of Spodoptera frugiperda larvae is accomplished by utilizing B. thuringiensis bacteria. This study aims to determine the best B. thuringiensis isolate for killing S. frugiperda larvae, to identify the most effective concentration of B. thuringiensis suspension, and to ascertain the mortality rate of S. frugiperda larvae. The design of this study was a randomized block design (RBD) with two factors. The first factor was the type of B. thuringiensis isolate, consisting of four isolates. Larval mortality data were analyzed using Analysis of Variance (ANOVA) and further tested with the Duncan test. The results obtained showed the highest average mortality of S. frugiperda larvae using isolates Bt3BP14 and Bt4TSR6. These two isolates had the highest average mortality on the third day. Bt3BP14 and Bt4TSR6 isolates have high potential in controlling S. frugiperda larvae, with an average mortality proportion of 86,67% and 66,67% observed over three days. The best doses of B. thuringiensis suspension for killing S. frugiperda larvae were 15 ml and 20 ml. It can be concluded that B. thuringiensis isolates can be used as natural biological control agents against S. frugiperda larvae that attack food crops and horticulture.
Aquatic Insect Diversity as Bioindicators of Water Quality in Organic Inorganic Rice Fields, Pringsewu, Lampung: Keanekaragaman Serangga Air sebagai Indikator Kualitas Air di Sawah Organik Anorganik di Pringsewu Lampung Pramudita, Rima; Dania Pratami, Gina; Meidaliyantisyah; Handayani, Kusuma
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 2 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/j-bekh.v12i2.424

Abstract

The diversity of aquatic insects serves as an important indicator for assessing the quality of agricultural ecosystems, particularly in organic and conventional (non-organic) rice fields. Organic farming, which relies on natural inputs, tends to support greater biodiversity, whereas conventional farming may reduce it due to the use of chemical substances.   This   study   aimed   to   examine   the diversity, dominance, evenness, and environmental factors influencing the presence of aquatic insects in two types of agricultural systems in Tulung Agung Village, Lampung. The results showed that the organic   rice   fields   had   higher   aquatic   insect diversity, with a diversity index (H') of 2.3386, a dominance index (D) of 0.058, and an evenness index (E) of 0.9411. In contrast, the conventional rice fields showed a diversity index (H') of 0.9743, a dominance index (D) of 0.5188, and an evenness index (E) of 0.3312. Environmental factors had no significant effect on aquatic insects in the organic fields, but showed a strong correlation in the conventional fields, with a correlation value (r) of 0.905 and a significance value (p) of 0.035. These findings highlight that organic farming systems are better able to support a more stable aquatic ecosystem balance.
Mortality and Morphological Changes in Aedes aegypti Larvae by Bacillus thuringiensis and Serratia marcescens: Mortalitas dan Perubahan Morfologi Larva Aedes aegypti oleh Bacillus thuringiensis dan Serratia marcescens Yulia Sari, Umi; Rosa, Emantis; Handayani, Kusuma; Busman, Hendri
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 12 No. 2 (2025)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

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Abstract

The use of biolarvicides is an alternative biological agent to replace synthetic insecticides, namely entomopathogenic bacteria. The purpose of this study was to determine the potential of Bacillus thuringiensis and Serratia marcescens extracts on mortality and morphological changes in Aedes aegypti larvae. This study was a 2-factor experimental study using a Randomized Block Design (RBD). The first factor, the type of entomopathogenic bacteria, namely B. thuringiensis and S. marcescens. The second factor, the concentration of bacterial extracts (60 mL, 70 mL, 80 mL), negative control (sterile distilled water). Mortality data were processed using ANOVA at the 5% level. The results showed that the administration of B. thuringiensis and S. marcescens extracts caused mortality and morphological changes in Ae. aegypti larvae. B. thuringiensis at concentrations of 60 mL, 70 mL, and 80 mL caused 100% larval mortality, while S. marcescens at concentrations of 60 mL resulted in 70%, 53%, and 50% at concentrations of 70 mL and 80 mL, respectively. Morphological changes in larvae were indicated by changes in color, texture, and damage to the digestive system. It was concluded that B. thuringiensis has the highest potential as an entomopathogenic agent compared to S. marcescens in causing mortality and morphological changes in Ae. aegypti larvae.
Antibacterial Activity Test of Methanol Extract of Gracilaria sp. against Escherichia coli Using Disc Diffusion Method: Uji Aktivitas Antibakteri Ekstrak Metanol Gracilaria sp. terhadap Escherichia coli Dengan Metode Difusi Cakram Ajeng Pawesti, Ammanda Sadiva; Handayani, Kusuma; Setyaningrum, Endah; Busman, Hendri
BIO-EDU: Jurnal Pendidikan Biologi Vol. 10 No. 3 (2025): BIO-EDU: Jurnal Pendidikan Biologi - December 2025
Publisher : Jurusan Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jbe.v10i3.9389

Abstract

The utilization of marine resources as an alternative source of natural antibacterial agents has been increasingly developed in recent years. Gracilaria sp., a type of red algae, is known to contain biologically active compounds with potential antibacterial properties, particularly against Escherichia coli. This study aims to determine the antibacterial potential of methanol extract from Gracilaria sp. against E. coli using an experimental method with a Completely Randomized Design (CRD). Data were analyzed using One-Way ANOVA followed by Tukey's test. The stock solution of the extract was prepared at a concentration of 100 mg/mL and tested at concentrations of 0.05%, 1%, 5%, and 10%, with ciprofloxacin as the positive control and sterile distilled water as the negative control. The experiment was conducted with three replications. The results showed that the methanol extract of Gracilaria sp. formed inhibition zones at the highest concentrations of 10% and 5%, with an average diameter of only 0.075 mm. Thus, the methanol extract of Gracilaria sp. exhibits weak antibacterial potential against Escherichia coli, as the inhibition zone formed was significantly smaller than that of the positive control, which reached 1.7 mm.
Isolasi, Identifikasi, dan Bioaktivitas Jamur Endofit Akar Cabai Rawit sebagai Biokontrol dan Pemacu Pertumbuhan Wahid, Muhammad Zainul; Irawan, Bambang; Agustrina, Rochmah; Handayani, Kusuma
JURNAL BIOSHELL Vol 15 No 2 (2026): Article in Progress
Publisher : Universitas Islam Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56013/bio.v15i2.5603

Abstract

Penelitian ini dilakukan untuk mengisolasi serta mengarakterisasi jamur endofit dari jaringan akar cabai rawit (Capsicum frutescens L.), serta mengevaluasi bioaktivitasnya sebagai agen biokontrol terhadap C. capsici dan sebagai pemacu pertumbuhan tanaman. Isolasi dilakukan menggunakan metode kultur jaringan tanaman, diikuti dengan identifikasi secara makroskopis dan mikroskopis. Uji aktivitas biokontrol dilakukan melalui metode dual culture, sedangkan kemampuan produksi Indole Acetic Acid (IAA) diuji menggunakan reagen Salkowski. Pengujian in vivo dilakukan untuk menilai kemampuan isolat dalam menekan keparahan penyakit dan meningkatkan pertumbuhan tanaman cabai rawit. Hasil penelitian menunjukkan bahwa beberapa isolat jamur endofit memiliki aktivitas antagonis terhadap C. capsici, dengan salah satu isolat (E2) menunjukkan daya hambat tertinggi. Isolat tersebut juga mampu memproduksi IAA dan memberikan pengaruh positif terhadap pertumbuhan tanaman, yang ditunjukkan oleh peningkatan parameter tinggi tanaman, jumlah daun, dan berat basah. Selain itu, aplikasi isolat E2 secara nyata menurunkan tingkat keparahan penyakit dibandingkan kontrol. Hasil ini mengindikasikan bahwa jamur endofit berpotensi sebagai agen biokontrol sekaligus pemacu pertumbuhan tanaman cabai rawit yang ramah lingkungan. Kata kunci: Jamur endofit, Biokontrol, Colletotrichum capsici, IAA, Cabai rawit
Co-Authors Abdullah Setiawan, Wawan Ajeng Pawesti, Ammanda Sadiva Andi Setiawan Arifiyanto, Achmad Ayuandira, Niken Bambang Irawan Damayanti, Berliana Dania Pratami, Gina Dermiyati, Dermiyati Elly Lestari Rustiati Endah Setyaningrum, Endah Firmansyah, Alvian Gina Dania Pratami, Gina Dania Hendri Busman Indriyani Indriyani John Hendri Krisna Rendi Awalludin Mayuri, Nindya Sekar Meidaliyantisyah Mirawanty Amin Musa Nainggolan, Ribka Debora Pamela, Ahmad Bintang Pramana, Yanuar Sigit Pramudita, Rima Pratiwi, Ratih - Prihadhi, Eka Putri, Meishy Handerlin Ramadhani, Winih Sekaringtyas Ratih, Cindy Lukyta Riandani Azzahra, Syifa Rochmah Agustrina Rosa, Emantis Rumidatul, Alfi Setiawan, Wawan A Sri Yusnaini Sugeng Triyono Sumardi Sumardi . Supriyanti, Arni Susanto, Gregorius Nugroho Tugiyono Tugiyono Tundjung Tripeni Handayani, Tundjung Tripeni Wahid, Muhammad Zainul WAWAN ABDULLAH SETIAWAN, WAWAN ABDULLAH Wulandari, Adelia saviera Yulia Sari, Umi