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All Journal Jurnal Bios Logos Protobiont: Jurnal Elektronik Biologi Tengkawang : Jurnal Ilmu Kehutanan AL KAUNIYAH BERITA BIOLOGI BIOMA BIOMA : Jurnal Biologi Makassar Jurnal Biologi Tropis IJIS - Indonesian Journal On Information System JURNAL PENGABDI Biosaintifika: Journal of Biology & Biology Education INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Teknologi Informasi dan Pendidikan Agroprimatech Biologica Samudra Sciscitatio : Journal for Biological Science Jurnal Borneo Akcaya : Jurnal Penelitian dan Pengembangan Pelayanan Publik Bioscientist : Jurnal Ilmiah Biologi Seminar Nasional Peningkatan Mutu Pendidikan Life Science Indonesian Community Journal JAMAS: Jurnal Abdi Masyarakat Life Science Berita Biologi
Rikhsan Kurniatuhadi, Rikhsan
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Tanjungpura, Pontianak, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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 1 
Kualitas Gaharu Aquilaria sp. dengan Pemberian Bioinokulan Fermentasi Batang Pisang yang Terkena Penyakit Layu Fusarium selno, Silvester; Zakiah, Zulfa; Kurniatuhadi, Rikhsan
JURNAL BIOS LOGOS Vol 11, No 2 (2021): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.11.2.2021.32551

Abstract

(Article History: Received February 1, 2021; Revised March 8, 2021; Accepted April 7, 2021) ABSTRAK Upaya produksi gaharu budidaya umumnya menggunakan inokulan dari jenis Fusarium sp., namun mahalnya inokulan biakan murni menjadi faktor pembatas untuk produksi gaharu budidaya. Bioinokulan dapat menjadi solusi bagi petani gaharu karena dibuat dari bahan yang mengandung mikroorganisme patogen dan tidak membutuhkan proses pembiakan murni. Penelitian ini bertujuan untuk mengidentifikasi jenis-jenis mikroorganisme dan konsentrasi bioinokulan yang tepat digunakan untuk menghasilkan kualitas gaharu yang baik serta mengetahui kualitas gaharu yang dihasilkan dengan menggunakan bioinokulan dari fermentasi batang pisang yang terkena penyakit layu fusarium. Metode penelitian lapangan dengan melakukan inokulasi bioinokulan dari fermentasi batang pisang yang terkena penyakit layu fusarium pada pohon Aquilaria sp. Setiap konsentrasi diinokulasikan pada pohon yang berbeda. Pada ketinggian 30 cm dari permukaan tanah, pohon dibuat lubang sebanyak 5 buah secara vertikal dengan menggunakan bor dengan jarak antar lubang 50 cm. Inokulan diinokulasikan melalui lubang yang telah dibuat dengan metode infus. Hasil penelitian diperoleh tiga jenis mikroorganisme yang terdapat pada bioinokulan yaitu kelompok genus Aspergillus, Fusarium dan Saccharomyces. Perlakuan dengan konsentrasi bioinokulan 140 ml memberikan hasil yang terbaik terhadap bobot kayu gaharu, warna, aroma dan kadar resin gaharu yang dihasilkan. Berdasarkan penelitian ini dapat disimpulkan bahwa kualitas gaharu yang dihasilkan setelah 4 bulan inokulasi menghasilkan mutu gaharu kelas kemedangan. Kata Kunci: Aquilaria sp., Bioinokulan, Fusarium sp., Gaharu, Inokulasi ABSTRACT Efforts to produce cultivated agarwood generally use inoculants of the type Fusarium sp. however the high cost of pure culture inoculants is a limiting factor for cultivated agarwood production. Bioinoculants can be a solution for agarwood farmers because they are made from materials containing pathogenic microorganisms and do not require a pure breeding process. The research aims to determine the kind of microorganisms and the proper concentration of bioinoculants to produce good quality agarwood and determine the quality of aloes produced by using bioinoculants from fermented banana stem affected by fusarium wilt disease. The method of field research was by conducting inoculation of bioinoculants from the fermentation of banana stems affected by fusarium wilt on Aquilaria sp. Each concentration was inoculated on a different tree. At a height of 30 cm from the ground, 5 holes are made vertically using a drill with a distance of 50 cm between the holes. The inoculant is inoculated through the hole that has been made by the infusion method. The results showed that there were three types of microorganisms found in bioinoculants, namely the Aspergillus, Fusarium and Saccharomyces genus groups. Treatment with a concentration of 140 ml of bioinoculant gave the best results on the weight of agarwood, color, aroma and content of the resulting agarwood resin. Based on this research, it can be concluded that the quality of aloes produced after 4 months of inoculation resulted in the quality of agarwood in the kemedangan class.Keywords: Agarwood, Aquilaria sp., Bioinoculants, Fusarium sp., Inoculation
Uji Antagonis Pseudomonas flourescens spp. Terhadap Isolat Bakteri Xanthomonas (SL3) dari Daun Padi Bergejala Hawar di Kabupaten Kubu Raya Larasaty, Suprih; Mukarlina, Mukarlina; Kurniatuhadi, Rikhsan
JURNAL BIOS LOGOS Vol 11, No 1 (2021): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.11.1.2021.30998

Abstract

(Article History: Received 21 October 2020; Revised 5 November 2020; Accepted 11 November 2020) ABSTRAKPenyakit hawar pada tanaman padi disebabkan oleh bakteri anggota genus Xanthomonas. Pengendalian penyakit tanaman dapat menggunakan bakteri sebagai Agen Pengendalian Hayati (APH) yang bersifat antagonis terhadap patogen. Pseudomonas flourescens merupakan APH yang memiliki kemampuan penghambatan terhadap bakteri patogen Xanthomonas. P. flourescens spp yang diisolasi dari rhizosfer berbeda memiliki kemampuan penghambatan yang berbeda. Penelitian ini bertujuan untuk menguji kemampuan daya hambat bakteri P. flourescens spp. yang berasal dari rhizosfer yang berbeda terhadap bakteri Xanthomonas yang diisolasi dari daun padi bergejala hawar di Kabupaten Kubu Raya. Penelitian ini dilakukan pada bulan Mei 2019 bertempat di Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak. Pengambilan sampel dilakukan di sentra produksi padi Desa Limbung Kabupaten Kubu Raya. Uji antagonis dilakukan di Laboratorium Mikrobiologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak menggunakan metode Kirby-bauer. Hasil uji antagonis menunjukkan kedua bakteri P. flourescens spp. mampu menghambat isolat bakteri genus Xanthomonas. Jenis P. flourescens spp (Sgu5) memiliki kemampuan penghambatan terbaik hingga mencapai 8,43 mm.Kata kunci: Agen pengendalian hayati; Penyakit Hawar; Pseudomonas flourescens; Xanthomonas (SL3) ABSTRACTBlight disease in rice plants is caused by bacteria belonging to the genus Xanthomonas. Plant disease control can use the Biological Control Agent (APH) which is antagonistic to pathogens.Pseudomonas flourescens is an APH that has the ability to inhibit the pathogenic bacteria Xanthomonas. Pseudomonas flourescens spp. isolated from different rhizosphere had different inhibitory abilities. This study aims to determine the inhibitory ability of the bacteria Pseudomonas flourescens spp. against bacteria isolated from blight with symptomatic rice in Kubu Raya Regency. The research was conducted in my 2019 at the Biology Department, Faculty of Mathematics and Natural Sciences, Tanjungpura University Pontianak. Sampling was carried out at the rice production center in Limbung Village, Kubu Raya Regency.The antagonist test was carried out at the Microbiology Laboratory of the Faculty of Mathematics and Natural Sciences, Tanjungpura University, Pontianak using the Kirby-bauer method. The antagonist test result showed that both Pseudomonas flourescens spp. able to inhibit bacterial isolates of the genus Xanthomonas. Pseudomonas flourescens spp. (Sgu5) has the best inhibition ability up to 8.43 mm.Keywords: Biological control agent; Blight disease; Pseudomonas flourescens; Xanthomonas (SL3)
Antibacterial Activity of Simpur Leaves Methanol Extract (Dillenia sp.) Againts Staphylococcus aureus Shafira Qurrata A'yuni; Rikhsan Kurniatuhadi; Zulfa Zakiah
JURNAL BIOS LOGOS Vol. 15 No. 1 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i1.54604

Abstract

Simpur (Dillenia sp.) is a plant that can be used as traditional antibacterial medicine. This study aims to determine the inhibitory activity of Staphylococcus aureus bacteria when exposed to a concentration of methanol extract from Simpur leaves (Dillenia sp.). This study utilized a completely randomized design with five concentration levels of Dillenia sp. specifically, the study included a control group and methanol extracts of Simpur leaves at concentrations of 5%, 10%, 15%, and 20%. The methods used included total plate count, hemocytometer, and UV-Vis spectrophotometry, which were then analyzed using ANOVA (Analysis of Variance). The results showed that the greatest inhibition occurred in the methanol extract of Simpur leaves (Dillenia sp.) at a concentration of 20%, there was a decrease in the number of colonies ranging from 90% to 94%, while the decrease in the number of cells ranged from 17% to 18%. The lowest inhibition was found in the methanol extract of Simpur leaves (Dillenia sp.) with a concentration of 10%, the proportion of decreasing the number of colonies ranged from 63% to 70%, while the proportion of decreasing the number of cells ranged from 6% to 7%. The highest optical density (OD) value at a 20% concentration ranged from 0.482 to 0.547, while the lowest value at a 5% concentration ranged from 0.127 to 0.131. The conclusion of this study is that the methanol extract of Simpur leaves (Dillenia sp.) Bacteriostatic antibiotics only inhibit bacterial growth and cannot kill bacteria.
Co-Authors Abdullah Abdullah Anzani, Yunita Magrima Apriani, Lisa Ari Hepi Ari Hepi Yanti Ari Hepi Yanti Ari Hepi Yanti Ari Hepi Yanti Ari Hepi Yanti Asrin, Fauzan Daniati, Eleutra Devinda Ekarizky Diputri Diah Wulandari Rousdy Dwi Gusmalawati Edi Kurniadi Eka Karunia Elvi Rusmiyanto Pancaning Wardoyo, Suci Lestari, Mukarlina, Fahruna, Yulyanti Firman Saputra Firman Saputra Hadary, Ferry Harfan, Donatus Tia Hendri Hendri Henno Wisnu Putra Holyness Nurdin Singadimedja Hosiana Patricia Sheren Indah Permatasari Indah Pratiwi Irwan Lovadi Ita Purnama Sari, Ita Purnama Julia Nanda Puspita Junardi Junardi Junardi Komara, Dina Kustiati Kustiati Kustiati Larasaty, Suprih Lastyanti Mulyani Lukmanul Hakim Muhammad Luthfi Mukarlina, Mukarlina Mulyani, Lastyanti Muthahhari, Morteza Muthahhari, Morthezza Naulandari, Mesi Rafdinal Rafdinal Rahmawati Rahmawati Rahmawati Rahmawati Rahmawati Rahmawati Rahmawati, Rahmawati Riyandi Riyandi Riyandi Riyandi Riza Linda Riza Linda selno, Silvester Shafira Qurrata A'yuni Siti Ifadatin Siti Ifadatin Siti Khotimah Sulistrioningsih, Sulistrioningsih Sulistyarini, Sulistyarini Tri Rima Setyawati Tri Rima Setyawati Tri Rima Setyawati Tri Rima Setyawati Turnip, Masnur Umaroh, Dian Asti Williem, Albertus Zulfa Zakiah Zulfa Zakiah