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Zulwanis, Zulwanis
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Uji Aktivitas Antibakteri Ekstrak Daun Ciplukan (Physalis angulata L.) Terhadap Bakteri Staphylococcus epidermidis Kulla, Periskila Dina Kali; Aldafi, Daevanul Aslam; Meilina, Rulia; Zulwanis, Zulwanis
JOURNAL OF HEALTHCARE TECHNOLOGY AND MEDICINE Vol 9, No 2 (2023): Oktober 2023
Publisher : Universitas Ubudiyah Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33143/jhtm.v9i2.3562

Abstract

Ciplukan (Physalis angulata L.) merupakan tanaman obat tradisional dari famili solanaceae sering dimanfaatkan dalam penyembuhan gangguan penyakit infeksi kulit. Daun Ciplukan (Physalis angulata L.) memiliki Aktivitas sebagai antimikroba pada pencegahan jerawat. Kandungan flavonoid dan saponin dalam daun ciplukan berperan penting sebagai permeabilitas dinding sel dan menimbulkan kematian sel. Daun ciplukan juga telah terbukti memiliki kandungan senyawa Flavonoid, saponin, tanin, dan glikosida. Tujuan dari penelitian ini adalah untuk melihat aktivitas antibakteri dan kadar hambat minimum (KHM) dan kadar bunuh minimum (KBM) ekstrak etanol daun ciplukan terhadap bakteri Staphylococcus epidermidis. Penelitian ini menggunakan metode difusi dan dilusi menggunakan kertas cakram dengan beberapa konsentrasi ekstrak etanol daun ciplukan yaitu 5%, 10%, 15%, 20% dan 25%. Tetrasiklin 0,25% sebagai kontrol positif dan Dimetil sulfoksida (DMSO) 1% sebagai kontrol negatif. Data hasil penelitian dianalisis dengan program elektrik computer yang dianalisis menggunakan komputerisasi sistem data (KSD) dengan hasil penelitian Diameter zona hambat serta kadar zona hambat minimum (KHM) dan kadar bunuh minimum (KBM) yang dihasilkan pada pengujian ekstrak daun ciplukan terhadap Staphylococcus epidermidis dengan konsentrasi 25 % mendapatkan nilai rata-rata sebesar 11,62 mm sebagai aktivitas antibakteri dan kadar hambat minimum (KHM). kesimpulan bahwa ekstrak daun ciplukan memiliki aktivitas dalam menghambat pertumbuhan bakteri Staphylococcus epidermis dengan kekuatan zona hambat dalam (kategori kuat), Namun kadar bunuh minimum (KBM) pada konsentrasi 25% belum mampu membunuh bakteri Staphylococcus epidermidis dengan pengujian >25% agar ekstrak mampu membunuh bakteri. Kata kunci: Daun Ciplukan, antibakteri, aktivitas, Staphylococcus epidermidisCiplukan (Physalis angulata L.) is a traditional medicinal plant from the solanaceae family often used in healing skin infection diseases. Ciplukan leaves (Physalis angulata L.) have antimicrobial activity in the prevention of acne. The flavonoids and saponins in ciplukan leaves play an important role as cell wall permeability and cause cell death. Ciplukan leaves have also been shown to contain flavonoids, saponins, tannins, and glycosides. The purpose of this study was to see the antibacterial activity and minimum inhibitory levels (KHM) and minimum kill levels (KBM) of ciplukan leaf ethanol extract against Staphylococcus epidermidis bacteria. This study uses diffusion and dilution methods using disc paper with several concentrations of ethanol extract of ciplukan leaves, namely 5%, 10%, 15%, 20% and 25%. Tetracycline 0.25% as positive control and Dimethyl sulfoxide (DMSO) 1% as negative control. The research data were analyzed with an electric computer program analyzed using a computerized data system (KSD) with the results of the study The diameter of the inhibition zone and the minimum inhibitory zone level (KHM) and the minimum kill level (KBM) produced in testing ciplukan leaf extracts against Staphylococcus epidermidis with a concentration of 25% getting an average value of 11.62 mm as antibacterial activity and minimum inhibitory levels.Keywords: Ciplukan leaves, antibacterial, activity, Staphylococcus epidermidis
Co-Authors Afrina, Nyak Yusfa Aldafi, Daevanul Aslam Astryna, Syarifah Yanti Azkia, Hanin Efendi Efendi Essy Harnelly Halimursyadah Halimursyadah Helmi Helmi Indra Indra Jauharlina Jauharlina Kesumawati, Kesumawati Marniati Marniati, Marniati Maryam Jamilah Meilina, Rulia Muhammad Resthu Musa, Suryani Muyassir Muyassir Nasrullah Nasrullah Periskila Dina Kali Kulla Pratama, Said Mirza Qhamal, Syamsul Rina Sriwati Samadi Samadi Saulie, Diffa Aprilydia Taufan Hidayat Zairin Thomy Zuraida Hanum