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All Journal Jurnal Kesehatan Bakti Tunas Husada JMM (Jurnal Masyarakat Mandiri) Manuju : Malahayati Nursing Journal Science Midwifery Community Development Journal: Jurnal Pengabdian Masyarakat Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Jurnal Abdi Kesehatan dan Kedokteran Jurnal Pengabdian Masyarakat (Jupemas) Jurnal Kesehatan Bakti Tunas Husada
Yane Liswanti, Yane
Prodi D3 Analis Kesehatan, Universitas Bakti Tunas Husada
Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary Other

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Development of a natural eosinophil stain based on cordyline fruticosa leaves extract and selenicereus monacanthus (dragon fruit peel extracts) for hematology diagnostic applications Hasanah, Annisa Nur; Liswanti, Yane; Kusmiati, Meti; Salsabila, Aliya Fitria; Prihartini, Linda
Science Midwifery Vol 13 No 6 (2026): February: Health Sciences and related fields
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v13i6.2268

Abstract

The search for natural alternatives to conventional hematological stains has gained attention due to issues of dependency on imported reagents, cost, and potential environmental impact. This study aimed to explore the potential of Cordyline fruticosa (andong leaves) and Selenicereus monacanthus (dragon fruit peel) extracts as natural eosinophil stains in hematology. Pigment extraction was performed using standard procedures, followed by phytochemical screening for anthocyanins and flavonoids. The total anthocyanin content was higher in C. fruticosa (1.20 mg/L) compared to S. monacanthus (0.30 mg/L), while relative flavonoid absorbance values also indicated greater pigment density in andong leaves. Application of the extracts in the hemocytometer method demonstrated staining of the background and cellular components, although with weaker intensity than conventional eosin. In peripheral blood smear preparations, Giemsa stain produced the most distinct results, clearly differentiating eosinophil nuclei, cytoplasm, and characteristic granules. In contrast, the natural extracts yielded paler orange cream staining, with less clarity and limited granule visualization, and some smears exhibited detachment due to imperfect fixation. These findings suggest that while andong leaves and dragon fruit peel contain bioactive pigments with staining potential, their application in hematology requires further optimization, particularly in fixation methods, extract concentration, and stability testing over longer storage periods. 
Co-Authors Afiah, Irma Nur Anggun Anggun Dewi Peti Virgianti, Dewi Peti Farah Farah Faulina, Farah Ferdina, Dina Harnanik Nawangsar Hasanah, Annisa Nur Hemarisa Nurizkillah Hendro Kasmanto Hendro Kasmanto Henny Fitriani Henny Fitriani, Henny Ilham Alifiar Khusnul, Khusnul Korry Novitriani Latisa, Alsya Liliek Pratiwi Marhami, Azwan Cahya Meisya F, Lisna Meri, Meri Meri, Meri Meti Kusmiati Meti Kusmiati Meti Kusmiati, Meti Muhammad Sugih Nata, Septiawan Dwi Naufal Naufal Pratiwi, Liliek Prihartini, Linda R. Suhartati Rianti Nurpalah Rianti Nurpalah, Rianti Rochmanah Suhartati RUDY HIDANA, RUDY Safitri, Lisdiana Pavila Salsabila, Aliya Fitria Sudianto, Sudianto Sugih, Muhamad Tanendri Arrizqiyani Tanendri Arrizqiyani, Tanendri Yayah Syafariah, Yayah