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Journal : Jurnal Airaha

Karakteristik Bakteri Streptococcus agalactiae NP104O, S01-196-16 dan NMbO Penyebab Streptococcosis pada Ikan Nila (Oreochromis niloticus) Achmad Suhermanto; Herdianto T; Suhermin; Ridwan; Nurmawanti I
Jurnal Airaha Vol 8 No 02: DEC 2019
Publisher : Politeknik Kelautan dan Perikanan Sorong, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (262.599 KB) | DOI: 10.15578/ja.v8i02.138

Abstract

Failure of tilapia culture in Indonesia in the hatchery, nursery and rearing segments mostly is caused by Streptococcus agalactiae infection. This study aimed to detect the characteristics of bacteria S. agalactiae NP104O, S01-196-16 and NMbO which infect tilapia in aquaculture. Bacterial isolation was carried out during an outbreak of streptococcosis, further analysis based on morphological and biochemical characters. The pathogenicity test was done by injecting bacteria intraperitoneally at a dose of 107 CFU fish-1. The results showed that all isolates was cocci, gram positive, negative catalase and negative oxidase. The three isolates had different concentrations and growth patterns until end of the observation. Pathogenicity test results obtained all fish injected with NP104O, S01-196-16 and NMbO bacteria appeared as clinical symptoms of melanosis, unilateral or bilateral exophthalmos, turbidity in the eye, curved vertebrae forming the letter C, erratic, and whirling. Hopefully, the results of this study can be used as a reference for prevention of streptococcosis infections in tilapia.
The potential of Barringtonia asiatica Biopesticide from Papua to Eradicate Pests in Aquaculture Achmad Suhermanto; Fabian Ardianta; Murtihapsari Murtihapsari; Achmad Sofian
Jurnal Airaha Vol 11 No 01: June 2022
Publisher : Politeknik Kelautan dan Perikanan Sorong, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.155 KB) | DOI: 10.15578/ja.v11i01.335

Abstract

Saponins are compounds derived from plants that are currently widely used in aquaculture for aquatic pest control. In this study, potential saponin components extracted from fish poison tree (Barringtonia asiatica), which is widely dispersed in tropical areas worldwide, were characterized. Saponin properties were obtained from seeds and leaves by extraction and spectrophotometric methods. To test its properties, four different concentrations of saponin extracts, namely 10 ppm, 20 ppm, 30 ppm, and 40 ppm, were applied in four trials containing 20 tilapias each. An unexpected result was obtained and proved that the saponins extracted from the seeds of the fish poison tree proved to be significantly more effective than the saponins extracted from the peel of its fruit to eradicate pest in pond. The results also revealed that the lethal dose of saponins reached higher level at a concentration of 40 ppm. It can be concluded that fish poison tree as a poisonous tree plays an important role in ensuring the sustainability of saponin stocks. The use of natural materials such as fish poison tree as a biopesticide has the potential to minimize environmental damage and reduce costs for aquaculture.