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Synergistic Impact of Cholesterol-Loaded Cyclodextrin and Moringa Leaf Extract on Post-Thaw Boar Sperm Kinematics Hine, T. M.; Nalley, W. M.; Uly , K.; Kune , P.; Lawa , A. B.; Setyani , N. M. P.; Bette , Y. Y.; Banamtuan , A. N.; Riwu , R. M. J.
Tropical Animal Science Journal Vol. 49 No. 1 (2026): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2026.49.1.30

Abstract

Structural and functional damage during cryopreservation usually impairs the quality of boar sperm, especially post-thaw motility and kinematic performance, which restricts its use in artificial insemination programs. Cholesterol-loaded cyclodextrin (CLC) and moringa leaf extract (MLE) are considered possible cryoprotective additives because they are membrane-stabilizing and antioxidants. The study investigated the possible synergistic effect of the Tris-egg yolk (TY) extender in combination with CLC and MLE on the motility and kinematics properties of thawed boar sperm, as identified in a Computer-Assisted Sperm Analysis (CASA) system. Ejaculates of four healthy boars were diluted with Tris-based extender without or with CLC (1 or 2 mg/mL), MLE (1 or 1.5 mg/mL), or both, and frozen with a conventional protocol. Kinematic variables alongside progressive and fast motility were included as post-thaw tests. The findings showed that combination treatment of 2 mg/mL CLC and 1 mg/mL MLE (T7) resulted in a significant increase in all sperm motility and kinematic variables compared to the control group (T0) (p<0.05). In particular, T7 had better progressive motility (38.04%), fast motility (22.42%), and higher kinematic variables, especially VCL (106.34 µm/s), VSL (36.28 µm/s), and VAP (45.41 µm/s), and better displacement and trajectory indices. T7 was always better than the single supplementation and other combinations. Finally, the addition of CLC (2 mg/mL) and MLE (1 mg/mL) into the Tris egg yolk extender could be a successful approach towards optimizing semen cryopreservation in boars.
PENYULUHAN PENGOLAHAN LIMBAH PETERNAKAN BABI MENJADI PUPUK BOKASHI RAMAH LINGKUNGAN David Agustinus Nguru; Ni Nengah Suryani; Simon E. Mulik; Alberth N. Ndun; Alvrado B. Lawa; Yustiani Y. Bette; Nitty C. Mafefa
JMM (Jurnal Masyarakat Mandiri) Vol 9, No 4 (2025): Agustus
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v9i4.30394

Abstract

Abstrak: Perkembangan usaha ternak babi di Nusa Tenggara Timur tidak di imbangi dengan pengolahan limbah peternakan yang baik. Pengelolaan limbah peternakan yang kurang baik dapat mencemari lingkungan, menjadi sarang penyakit dan berpotensi menicu konflik sosial. Program PKM (Pengabdian Kepada Masyarakat) bertujuan untuk mengatasi permasalah limbah peternakan babi yang di hadapi para peternak dengan pengelolaan menjadi pupuk bokasi untuk meningkatkan nilai guna, serta meningkatkan keterampilan softsskill dan hardskill. Pelaksanaan PKM dengan metode ceramah serta ekperimen langsung yang melibatkan peternak. Kegiatan ini dihadiri oleh 25 peternak babi. Evaluasi terhadap kegiatan ini dilakukan dengan menerapkan pre-test yang terdiri dari 15 soal, yang dilaksanakan sebelum kegiatan dimulai, dan post-test yang dilaksanakan setelah kegiatan selesai. Program PKM bertujuan membantu peternak dalam proses pengelolaan limbah peternakan babi menjadi pupuk bokasi, sehingga dapat meningkatkan nilai guna dan manfaatnya. Hasil evaluasi yang dilakukan pada 25 peternak menunjukkan bahwa peningkatan tertinggi terdapat pada kemampuan menilai kualitas pupuk bokashi (88%), yang menunjukkan pemahaman peserta dalam mengenali ciri-ciri pupuk yang berhasil.Abstract: The development of pig farming in East Nusa Tenggara is not balanced with good livestock waste treatment. Poor management of livestock waste can pollute the environment, become a nest of diseases and potentially trigger social conflicts. The PKM (Community Service) program aims to overcome the problem of pig farm waste faced by farmers by managing it into bokasi fertilizer to increase the use value, as well as improve soft skills and hard skills. The implementation of PKM with lecture methods and direct experiments involving farmers. This activity was attended by 25 pig farmers. The evaluation of this activity was carried out by applying a pre-test consisting of 15 questions, which was carried out before the activity started, and a post-test which was carried out after the activity was completed. The PKM program aims to assist farmers in the process of managing pig farm waste into bokasi fertilizer, so that it can increase its use value and benefits. The results of the evaluation conducted on 25 farmers showed that the highest improvement was in the ability to assess the quality of bokashi fertilizer (88%), which showed the participants' understanding in recognizing the characteristics of successful fertilizers.
Correlation Between Chest Girth, Body Length, and Body Height on Wither Weight of Rote Sheep Ewes Setyani, Ni Made Paramita; Nalley, Wilmientje Marlene; Hine, Thomas Mata; Riwu, Agustinus Ridlof; Uly, Kirenius; Kune, Petrus; Bette, Yustiany Yuliana; Lawa, Alvrado Bire; Mulik, Simon Edison; Banamtuan, Adyanto Nessy; Riwu, Roni Markus Jami; Zulfa, Yanna Nailla; Listyadi, Rut
Journal of Tropical Animal Science and Technology Vol. 8 No. 1 (2026): Journal of Tropical Animal Science and Technology
Publisher : Animal Husbandry Study Program, Faculty of Agriculture, Timor University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jtast.v8i1.10617

Abstract

This study aimed to analyze the relationship between linear body measurements including chest girth (CG), body length (BL), and wither height (WH) and body weight (BW) in Rote sheep ewes across different age groups to determine the most reliable predictor of body weight. The research employed a quantitative method through direct field measurements and weighing. Data collected including vital statistics (CG, BL, WH) and BW from Rote sheep ewes categorized into three age groups (I0, I2, and I4). The data were analyzed statistically using correlation coefficient (r), the coefficient of determination (R²), and bias analysis to evaluate the accuracy of weight estimation. The results revealed that chest girth exhibited the highest correlation with body weight, particularly in the I0 group (r = 0.93; R² = 86.05%), indicating a strong positive relationship and high predictive accuracy. Body length showed a moderate relationship (r = 0.87; R² = 76.11%) in the same group, while wither height demonstrated a relatively weaker correlation (r = 0.73; R² = 53.94%). Across all age groups, the predictive strength of body measurements decreased with increasing age, suggesting that skeletal growth contributes less to body weight variation in mature sheep. In conclusion, chest girth was identified as the most accurate and reliable linear measurement for predicting body weight in Rote sheep ewes, particularly in younger age groups, and can therefore be used as a practical and non-invasive tool for estimating livestock weight.
Pengaruh Penambahan Sari Daun Pepaya Dalam Pengencer Sitrat Kuning Telur Terhadap Kualitas Spermatozoa Babi Persilangan Landrace Dan Duroc Apolonius Grad Suardi; Kirenius Uly; Aloysius Marawali; Yustiany Yuliana Bette
Journal of Innovative and Creativity Vol. 5 No. 3 (2025)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v5i3.4124

Abstract

Tujuan penelitian ini untuk mengetahui pengaruh penambahan sari daun papaya (SDP) ke dalam pengencer Tris-kuning Telur (T-KT) terhadap kualitas semen babi persilangan landrace dan duroc. Materi yang digunakan adalah semen segar dari babi jantan persilangan landrace dan duroc berumur 1,5 tahun dengan kondisi sehat dan terlatih untuk penampungan semen. Penelitian ini menggunakan metode eksperimental dengan rancangan acak lengkap yang terdiri atas enam perlakuan dan lima ulangan, yaitu: T-KT (P0), SDP, (P1) T-KT+0,5% SDP, (P2) T-KT+1% SDP, (P3), T-KT+1,5% SDP (P4), T-KT+2% SDP, dan (P5) TKT+2,5% SDP. Semua perlakuan disimpan dalam coolbox pada suhu 15–20ºC. Variabel yang dilihat adalah motilitas, viabilitas, abnormalitas, dan daya tahan hidup spermatozoa. Evaluasi dilakukan setiap 12 jam. Hasil penelitian menunjukkan bahwa perlakuan P5 (penambahan SDP 2,5%) pada penyimpanan selama 36 jam memberikan hasil terbaik (P<0,05) dibandingkan dengan perlakuan lainnya, dengan nilai motilitas 44,00±2,23% viabilitas 51,90±2,63%, abnormalitas 5,50±0,70%, dan daya tahan hidup selama 45,60 jam. Disimpulkan bahwa penambahan 2,5% SDP dalam pengencer Tris-kuning telur menghasilkan kualitas semen cair babi persilangan yang lebih baik dibandingkan perlakuan lainnya.
Quality Of Frozen Cement From Landrace Boars In Modified Citrate Diluents With Different Glycerol Concentrations Yohanes Paulus Martinus Bay; Thomas Mata Hine; Yustiany Yuliani Bette; W. Marlene Nalley
Journal of Innovative and Creativity Vol. 5 No. 3 (2025)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v5i3.4269

Abstract

The purpose of this study was to determine the quality of frozen semen from landrace boars in modified citrate diluents with different glycerol concentrations. The research material used was fresh cement from 2-3 year old landrace boar studs from 4 boars. This study used an experimental study with a completely randomized design method consisting of 4 treatments and 4 replications. The treatments in question are T1: S-KT + G 3%, T2: S-KT + G 5%, T3: S-KT + G 7% and T4: S-KT + G 9%. The diluted cement was stored at room temperature (27-28℃) for two hours (holding time), then centrifuged at 3000 RPM for 15 minutes. After centrifugation, the supernatant was discarded and the centrifuged pellet was re-diluted with basic diluent. The diluted semen was packed in 0.5 Ml straws, then packed, arranged in a freezing rack, and then equilibrated at 3-5℃ for 2 hours. Then the straws were frozen above the surface of liquid N2 with a distance of 10 cm at a temperature of ±-110℃ for 10 minutes. The results of this study indicate that the addition of different glycerols did not have a significant effect (P>0.05) on the motility, viability, abnormality and recovery rate of spermatozoa. It was concluded that the addition of different glycerols had the same impact but were not suitable for IB.