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Journal : journal of innovative and creativity

Pengaruh Penambahan Sari Daun Pepaya Dalam Pengencer Sitrat Kuning Telur Terhadap Kualitas Spermatozoa Babi Persilangan Landrace Dan Duroc Apolonius Grad Suardi; Kirenius Uly; Aloysius Marawali; Yustiany Yuliana Bette
Journal of Innovative and Creativity Vol. 5 No. 3 (2025)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v5i3.4124

Abstract

Tujuan penelitian ini untuk mengetahui pengaruh penambahan sari daun papaya (SDP) ke dalam pengencer Tris-kuning Telur (T-KT) terhadap kualitas semen babi persilangan landrace dan duroc. Materi yang digunakan adalah semen segar dari babi jantan persilangan landrace dan duroc berumur 1,5 tahun dengan kondisi sehat dan terlatih untuk penampungan semen. Penelitian ini menggunakan metode eksperimental dengan rancangan acak lengkap yang terdiri atas enam perlakuan dan lima ulangan, yaitu: T-KT (P0), SDP, (P1) T-KT+0,5% SDP, (P2) T-KT+1% SDP, (P3), T-KT+1,5% SDP (P4), T-KT+2% SDP, dan (P5) TKT+2,5% SDP. Semua perlakuan disimpan dalam coolbox pada suhu 15–20ºC. Variabel yang dilihat adalah motilitas, viabilitas, abnormalitas, dan daya tahan hidup spermatozoa. Evaluasi dilakukan setiap 12 jam. Hasil penelitian menunjukkan bahwa perlakuan P5 (penambahan SDP 2,5%) pada penyimpanan selama 36 jam memberikan hasil terbaik (P<0,05) dibandingkan dengan perlakuan lainnya, dengan nilai motilitas 44,00±2,23% viabilitas 51,90±2,63%, abnormalitas 5,50±0,70%, dan daya tahan hidup selama 45,60 jam. Disimpulkan bahwa penambahan 2,5% SDP dalam pengencer Tris-kuning telur menghasilkan kualitas semen cair babi persilangan yang lebih baik dibandingkan perlakuan lainnya.
Quality Of Frozen Cement From Landrace Boars In Modified Citrate Diluents With Different Glycerol Concentrations Yohanes Paulus Martinus Bay; Thomas Mata Hine; Yustiany Yuliani Bette; W. Marlene Nalley
Journal of Innovative and Creativity Vol. 5 No. 3 (2025)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/joecy.v5i3.4269

Abstract

The purpose of this study was to determine the quality of frozen semen from landrace boars in modified citrate diluents with different glycerol concentrations. The research material used was fresh cement from 2-3 year old landrace boar studs from 4 boars. This study used an experimental study with a completely randomized design method consisting of 4 treatments and 4 replications. The treatments in question are T1: S-KT + G 3%, T2: S-KT + G 5%, T3: S-KT + G 7% and T4: S-KT + G 9%. The diluted cement was stored at room temperature (27-28℃) for two hours (holding time), then centrifuged at 3000 RPM for 15 minutes. After centrifugation, the supernatant was discarded and the centrifuged pellet was re-diluted with basic diluent. The diluted semen was packed in 0.5 Ml straws, then packed, arranged in a freezing rack, and then equilibrated at 3-5℃ for 2 hours. Then the straws were frozen above the surface of liquid N2 with a distance of 10 cm at a temperature of ±-110℃ for 10 minutes. The results of this study indicate that the addition of different glycerols did not have a significant effect (P>0.05) on the motility, viability, abnormality and recovery rate of spermatozoa. It was concluded that the addition of different glycerols had the same impact but were not suitable for IB.