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All Journal Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. Majalah Kedokteran Bandung Research Journal of Life Science Jurnal Kebidanan Dan Kesehatan Tradisional MNJ (Malang Neurology Journal) Majalah Obstetri dan Ginekologi Biomedical Journal of Indonesia Medical Laboratory Technology Journal AcTion: Aceh Nutrition Journal Jurnal Ilmu Kedokteran dan Kesehatan Journal of Issues in Midwifery Asian Journal of Health Research
Wiyasa, I Wayan Arsana
Department Of Obstetrics And Gynaecology, Faculty Of Medicine, Universitas Brawijaya/ Saiful Anwar General Hospital, Malang, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : Medical Laboratory Technology Journal

 1 
Cowpea (Vigna unguiculata) Extract Reduce Malondialdehyde Levels and Prevent Aortic Endothelial Cell Decline in Ovariectomized Rats Ni Putu Sri Haryati; Elisa Danik Kurniawati; Tanti Tri Lestary; Eviana Norahmawati; I Wayan Arsana Wiyasa; Dwi Yuni Nur Hidayati; Tatit Nurseta
Medical Laboratory Technology Journal Vol. 7 No. 2 (2021): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.221 KB) | DOI: 10.31964/mltj.v0i0.402

Abstract

Estrogen has anti-oxidative and anti-inflammatory properties, but its levels decrease in postmenopausal women who can trigger oxidative stress. One of the most damaging effects of ROS is lipid peroxidation, and the end product is Malondialdehyde (MDA). Similarly, aging endothelium has increased oxidative stress and endothelial cell sensitivity to apoptosis. This study aimed to determine the effect of cowpea extract on serum MDA levels, aortic endothelial cell counts, and brain MDA levels in the ovariectomy model. Cowpea extract can be used as an alternative to prevent and overcome the effects that occur during menopause, such as cardiovascular problems, decreased bone mineral density, and dementia. The study used 15-month-old female Rattus norvegicus, divided into six groups (OVX, SHAM, OVX+estradiol, OVX+Vu 1.25; 2.5; and 5 mg/kg BW/day). Serum and brain MDA levels were examined by ELISA method, while the number of aortic endothelial cells were examined on histopathological preparations with Hematoxylin & Eosin (HE) staining. The mean value of serum and brain MDA levels decreased with an increase in the dose given (p-value 0.016). The mean value of aortic endothelial cells between the dose groups did not significantly differ. However, the mean value showed an increasing trend as the dose of cowpea extract was given. The results of this study indicate that the extract of cowpea has the potential as an antioxidant to reduce serum and brain MDA levels, prevent a decrease in the number of aortic endothelial cells. As prevention, cowpea extract can be used as an antioxidant and consumed since premenopause to minimize problems that occur during postmenopause.
Effectiveness of Rosmarinus officinalis and Centella asiatica Nanoemulsions Against Caspase 3 Gestational Diabetes Mellitus Expression Sutini Lamadi; Fajar Dwi Prastiwi; Tri Yudani Mardining Raras; Husnul Khotimah; I Wayan Arsana Wiyasa
Medical Laboratory Technology Journal Vol. 9 No. 2 (2023): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v9i2.537

Abstract

Gestational diabetes mellitus (GDM) is closely related to oxidative stress conditions in insulin resistance conditions that increase Reactive Oxygen Species (ROS) against the body's defense antioxidant mechanism. Chronic complications due to hyperglycemia in patients with GDM increase BAX / BCL2 levels which then activates the change of procaspase 3 to caspase 3, an activator of apoptosis. This study aims to determine the effect of the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions on caspase 3 expressions in zebrafish models of gestational diabetes mellitus, with a posttest-only controlled group design. The samples were divided into five groups, namely K- (EM), K+(EM+3% Glucose), P1, P2 and P3 (3% Glucose + Combination of Rosmarinus officinalis and Centella asiatica 2.5μg/ml, 5μg/ml and 10μg/ml), which will be tested for PEPCK and Caspase expression at the age of 3dpf using Real Time-PCR. The results of the correlation test of the group with caspase 3 obtained (p = 0.045) negative direction. These results show that the combination of Rosmarinus officinalis and Centella asiatica nanoemulsion decreased Caspase 3 expression in zebrafish models of gestational diabetes mellitus. The combination of Rosmarinus officinalis and Centella asiatica nanoemulsion has the potential to reduce blood glucose levels and reduce the risk of apoptosis in gestational diabetes mellitus patients.
Rosmarinus officinalis and Centella asiatica Affect Interleukin-6 Zebrafish Larvae Induced High Glucose Prastiwi, Fajar Dwi; Lamadi, Sutini; Khotimah, Husnul; Wiyasa, I Wayan Arsana; Raras, Tri Yudani M.
Medical Laboratory Technology Journal Vol. 10 No. 1 (2024): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v10i1.538

Abstract

Gestational Diabetes Mellitus (GDM) is diabetes that occurs during pregnancy. GDM is characterized by hyperglycemia. Hyperglycemia causes an inflammatory response characterized by the excretion of pro-inflammatory cytokines, one of which is IL-6. Centella asiatica and Rosmarinus officinalis are known as anti-diabetic and anti-inflammatory. This study aims to study the effect of the combination of nanoemulsion Centella asiatica and Rosmarinus officinalis on the expression of Interleukin-6 in Zebrafish larvae induced by 3% glucose. Hyperglycemia is known from increased levels of Phosphoenolpyruvate carboxykinase in Zebrafish larvae. Phosphoenolpyruvate carboxykinase and Interleukin-6 expression were measured by conventional PCR method. The combination of Rosmarinus officinalis nanoemulsion and Centella asiatica was given with three different doses, namely at a concentration of 2.5, 5 and 10 µg/mL. The statistical analysis used was One Way ANOVA to measure Interleukin-6 expression levels. The results of this study prove that the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions reduces the expression of Interleukin-6. The dose closest to the negative control was at a concentration of 5 μg/mL, although the final result was statistically significant (p<0.05). In conclusion, combining nanoemulsion Centella asiatica and Rosmarinus officinalis with the right dose reduced levels of Interleukin 6 in zebrafish larvae induced by high glucose.
Co-Authors Achmad Hidayat Adriana, Kusuma Agus Hendra Al Rahmad Akhmalia, Qorry' Afyu Annissa Febriani Arianto, Onni Dwi Arifiandi, Maya Devi Asih, Aliyah Tresna Azahra, Mutiara Syifa Baihaqi, Irfani Bambang Rahardjo DAMAYANTI, ZUHRIA PUSPITA Darwitri, Darwitri Dewi Santosaningsih Dewi, Ratih Frastika Dian Kusumaningtyas, Dian Diani, Yuli Ayu Donny Wisnu Wardhana Dwi Yuni Nur Hidayati Edy Mustofa Een Nuraenah Effendi, Madina Paramitha Elisa Danik Kurniawati Evi Zahara Eviana Norahmawati Fahimma Fahimma Fajar Dwi Prastiwi Fikhy Rizky Hapsari Gandhari, Anak Agung Sagung Agung Ayuska Hariyati, Suheni Ninik Heni Pujiastuti Husnul Khotimah Husnul Khotimah Husnul Khotimah I Wayan Agung Indrawan I Wayang Agung Indrawan Irfani Baihaqi Kalsumy, Umi Kartika Agustina Khoir, Safa’atul Kusuma Adriana Lamadi, Sutini Machlusil Husna Maharani Maharani Maria Antonia Barbara Batu Mali Mohammad Muljohadi Ali Nany Nita Noviana Ni Putu Sri Haryati Nina Rini Suprobo Nirwan Nugraha Noryanto, Mukhamad Nugrahanti Prasetyorini Nurdiana Nurdiana Nurlaili Ramli Ovinery, Amarsya Paramita, Galuh Anggarda Prastiwi, Fajar Dwi Puspita Handayani Qonita Prasta Agustia Raras, Tri Yudani M. Siskanita Nur Fitriana Sri Andarini Sri Winarsih Subandi Subandi Suheni Ninik Hariyati Sulistomo, Hikmawan Wahyu Sumarno Reto Prawiro Suseno, Daniel Alexander Sutini Lamadi Sutrisno Sutrisno Sutrisno Sutrisno Suwana, Febie Putra Syah Sembung Wasiso Tanti Tri Lestary Tatit Nurseta Teguh Wiyono Tri Yudani Mardining Raras Tri Yuliyani Wibisono, Hermawan Wino Vrieda Vierlia Yahya Irwanto Zahrani, Safira Astri Atika