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All Journal HAYATI Journal of Biosciences Jurnal Gizi dan Pangan Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Indonesian Journal of Pharmaceutical Science and Technology Prosiding Seminar Biologi INDONESIAN JOURNAL OF PHARMACY Journal of Medicine and Health Journal of Dentomaxillofacial Science Argipa (Arsip gizi dan Pangan) ARSHI Veterinary Letters Jurnal Ilmu dan Teknologi Kayu Tropis Pharmacoscript JURNAL KAJIAN VETERINER Britain International of Exact Sciences Journal (BIoEx Journal) Jurnal Pusat Inovasi Masyarakat Jurnal Kedokteran Hewan Journal of Medicine and Health

Vetnizah Juniantito

Institut Pertanian Bogor

Author-ID : 729418

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Veterinary

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Effectiveness of the application of bawang dayak (eleutherine palmifolia l. merr) extracts on healing proccess in the osteitis alveolar post tooth extraction through fibroblast examination, collagen density and amount of osteogenesis (experimental study) Fajar Rezandaru; Endang Syamsudin; Indra Hadikrishna; Vetnizah Juniantito
Journal of Dentomaxillofacial Science Vol. 5 No. 1 (2020): (Available online: 1 April 2020)
Publisher : DiscoverSys Inc.

Objectives:Â The aim of this study was to analyze the potential for topical administration of Eleutherine palmifolia (l.)Â merr extract gel in the healing proccess of wound with osteitis alveolar as a complication after tooth extraction in SpragueÂ dawley mice by observing fibroblast cell area, collagen density, and amount of osteogenesis.Material and Methods:Â This study used a true experimental research method conducted on Sprague Dawley mice. Sample were divided into 3 groups, namely K1 (osteitis alveolarÂ was not given any treatment), K2 (osteitis alveolarÂ mice were given iodoform paste application every 3 days), and K3 (osteitis alveolarÂ mice were given application of the extract gelÂ Eleutherine palmifolia (l.)Â merrÂ topically). On day 3, 5 and 10 necropsy was done and the tooth sockets in the form of soft tissue together with hard tissue was taken, then preparations were made and stained with masson trichrome to examined fibroblasts, collagen and osteogenesis.Results:Â Topical application of the extract gel Eleutherine palmifolia (l.)Â merr showed the mean value of fibroblasts area and collagen density and the amount of osteogenesisÂ were comparable to iodoform paste in alveolar osteitis healing.Conclusion:Â this study is that the application of Eleutherine Palmifolia (l) merr extract gel has almost the same effectiveness as iodoform paste

Stabilitas Fisikokimia dan Sifat Antipenuaan Kulit Formula Krim Berbahan Aktif Alami (Physicochemical stabilities and Skin Antiaging Properties of Bioaktive-based Cream Formulas) Rita K Sari; Nyoman J Wistara; Deded S Nawawi; Nopen Meisaroh; Ietje Wientarsih; D R Agungpriyono; L N Sutardi; Mawar Subangkit; Vetnizah Juniantito
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 15, No 1 (2017): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

The purpose of this study was to analyze the physicochemical stabilities and in vivo antiaging properties of the cream formulas of F1, F2, and F3 which contained the active compound of 0.1% 0.18, and 0.36%, respectively. The active compound was the combination of ethyl acetate fraction of Toona sinensis, Centella asiatica, and Acacia mangium leaf (1:2:1). For physicochemical stability tests (color, odor, acidity, and antioxidant activity), the creams were stored for 21 days in the refrigerator, room temperature, and extreme temperature (±40 ⁰C). For in vivo antiaging test, the mice skin were smeared with cream and UV irradiated for 15 minutes per day. After two and four weeks, the mice skins were biopsied. The results showed that the physicochemical properties of creams stored in the refrigerator were stable. The storage of creams at room and extreme temperature decreased the physicochemical properties. The application of cream formulas on mice skin after two to four weeks were able to increase the thickness of epidermis, but has not been able to increase the collagen skin of mice.Keywords: Acacia mangium, Centella asiatica, ethyl acetate fraction of leaf, physicochemical and antiaging properties of cream, Toona sinensis

Aktivitas Antioksidan Ekstrak Daun Surian, Mangium, dan Pegagan serta Kombinasinya dalam Formula Krim (Antioxidant Activities of Mangium, Surian, and Pegagan Leaf Extracts and Its Combination in Cream Formulas) Rita K Sari; Nyoman J Wistara; Deded S Nawawi; Itje Wintarsih; Dewi R Agungpriyono; L N Sutardi; Mawar Subangkit; Vetnizah Juniantito
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 14, No 2 (2016): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

The simplicia properties of toona (TS), mangium (AM), and centella (CA) leaves and antioxidant activities of their ethanolic leave extract and their combinations in cream formulas were examined in the present reserach. The ethanolic extracts were partitioned with n-hexane and ethyl acetate. The antioxidant activity of the fractions and their combinations were tested against DPPH. The best combination was formulated in F1, F2, and F3 cream formulas with 0.1, 0.18, and 0.36% of extract, respectively. The formulas antioxidant activity was then tested. The results showed that the water content, ash content, and acidic insoluble ash content of TS, AM, and CA simplicias were ±10%, 3.8-9.7%, and 0.9-1.2% respectively. The yields of n-hexane (FN), ethyl acetate (FEA), and residues (FR) fractions were 6-9, 2, and 5-6% respectively. The antioxidant activity of FEA, FR, and FN was very strong, strong, and weak to inactive, respectively. The best combination was the mixture of 2/3CA, 1/6AM, and 1/6TS (IC50 9 μg ml-1). The antioxidant activity of all cream formulas were higher than refered commercial cream. The IC50 values of formula 1, 2, and 3 were 1109, 596, and 202 μg ml-1, respectively, while the IC50 of refered commercial cream was 2372 μg ml-1.Keywords: antioxidant, centella, cream, extract, mangium, toona

Pathology of cutaneous blastomycosis in a cat Vetnizah Juniantito; Eva Harlina; . Jumari; Vici Eko Handayani; Iis Ismawati
ARSHI Veterinary Letters Vol. 4 No. 1 (2020): ARSHI Veterinary Letters - Februari 2020
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Cats are common as pet animals in Indonesia and live in close vicinity of human neighborhood, which make them potential for transmitting diseases to human. Blastomycosis is infectious disease caused by Blastomyces spp yeast. Here we describe morphopathology of blastomycosis found in skin of a Persian Cat. Grossly, the lesion were characterized by ulcerated wounds and multiple subcutaneous small nodules (2-5 mm in diameter) in the base of tail. Skin biopsy was made and further processed for histopathology. Microscopically, the lesion consist of epidermal necrosis, dermatitis, with coalescing dermal granulomatous inflammation, characterized by epitheloid and foreign type giant cells infiltrates. Numerous spherical-shaped structures and pseudohyphae which are Periodic Acid Schiff (PAS)-positive consistent with yeast morphology were found within the granulomas; morphology is also consistent with Blastomyces spp yeasts. Conclusively, multiple granulomatous dermatitis with evidence of Blastomyces yeasts is a hallmark of cutaneous blastomycosis. Exposure to Blastomyces spp yeast may possess infection threat to pet owners.

Effect of Boswellic Acid Administration on Blood Biochemical Profile of Sprague Dawley Rats in Cyclophosphamide-Induced Cardiotoxicity Conditions Miranti Fardesiana Putri; Ietje Wientarsih; Bayu Febram Prasetyo; Vetnizah Juniantito
Britain International of Exact Sciences (BIoEx) Journal Vol 4 No 2 (2022): Britain International of Exact Sciences Journal, May
Publisher : Britain International for Academic Research (BIAR) Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33258/bioex.v4i2.660

Cyclophosphamide (CP) is a chemotherapeutic agent that belongs to the alkylating agent group that is widely used in the treatment of cancer. Cardiotoxicity is often a side effect of using CP in medical therapy. In this study, 24 Sprague Dawley rats were randomly divided into 4 groups. Group 1 (K1) was given injection with aqua pro injection intraperitoneally (IP) once a week for 21 days. Group 2 (K2) was given IP CP with a dose of 50 mg/kg BW, once a week for 21 days. Group 3 (K3) was given boswellic acid extract at a dose of 250 mg/kg BW orally, every day for 21 days. Group 4 (K4) was given boswellic acid nanoparticles at a dose of 250 mg/kg BW orally, every day for 21 days. During the treatment the body weight of the rats was weighed every day. At the end of the treatment, the rats were euthanized and blood samples were taken for blood biochemical evaluation, namely CPK, LDH, AST, and ALT. The results showed that the levels of CPK, LDH, AST, and ALT in K2 were significantly higher (p<0.05) than K1, K3 and K4. Statistically, the results of CPK, LDH, AST and ALT in K3 and K4 were not significantly different (p<0.05) compared to K1. The two groups (K3 and K4) were not significantly different (p<0.05) but on average the CPK, LDH, AST, and ALT results in K4 had lower scores than K3. This can indicate the protective effect of boswellic acid and boswellic acid nanoparticles on the heart against cyclophosphamide-induced cardiotoxicity.

Potential Deleterious Effects of L-Citrulline Supplementation in Isoproterenol-Induced Myocardial Infarction: Focus on Nitrosative Stress Gregorius Bhaskara Wikanendra; Wawaimuli Arozal; Kusmardi Kusmardi; Vetnizah Juniantito; Andrea Laurentius
Indonesian Journal of Pharmacy Vol 30 No 4, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm30iss4pp269

L-Citrulline shows potential activity as a supplement to prevent myocardial infarction through vasodilative and possible antioxidative effects but may be deleterious by causing nitrosative stress. This study determined the potentially deleterious effects of L-citrulline supplementation in isoproterenol-induced myocardial infarction with a focus on nitrosative stress. L-Citrulline supplementation was given orally at dosages of 300 or 600mg/kg body weight daily for 6 days. Myocardial infarction was induced in Wistar rats via subcutaneous injection of isoproterenol (85 mg/kg body weight (BW)) on day 4 and 5. Blood pressure was measured at the end of the study (day 6) and rats were sacrificed to collect heart tissue samples for a histopathological evaluation. The histopathological evaluation was done using hematoxylin and eosin staining for the myocardial damage evaluation and immunohistochemical (IHC) staining of arginase-2, inducible nitric oxide synthase (iNOS), and 3-nitrotyrosine to evaluate nitrosative stress. L-Citrulline supplementation failed to show a significant protective effect on blood pressure and exacerbated the decrease of diastolic blood pressure. Both low and high dose L-citrulline supplementation had a significant protective effect on myocardial damage compared to the isoproterenol group (p<0.01). L-Citrulline also caused increased nitrosative stress as shown by increased expression of arginase-2 and 3-nitrotyrosine on IHC staining but tended to show an ameliorative effect on iNOS expression. A significant increase in arginase-2 expression was detected between the high dose group and the other groups (p<0.01 vs. normal and isoproterenol groups; p<0.05 vs. low dose group). L-Citrulline supplementation increased 3-nitrotyrosine expression in a dose-dependent manner, which was significantly different compared to the normal group (low dose: p<0.013; high dose: p<0.003). L-Citrulline increased the production of nitrosative stress but resulted in less myocardial damage through its other effects.

Identifikasi Penyebab Kematian Domba Melalui Bedah Bangkai di Desa Pantai Mekar, Muara Gembong, Kabupaten Bekasi Sharon Aurellia; Armando Balses Ishimora; Dwi Rofifah Putri Jania; Putri Ramadhanty; Aisyah Aisyah; Alfina Zalfa Suryono; Rafida Nisa Maghfiroh; Siti Magfiroh; Antonius Anre Sianturi; Ranady Perwira Nuruzzaman; Akhmad Endang Zainal Hasan; Vetnizah Juniantito
Jurnal Pusat Inovasi Masyarakat (PIM) Vol. 5 No. 1 (2023): April 2023
Publisher : Direktorat Pengembangan Masyarakat Agromaritim, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpim.5.1.116-127

Sheep breeders generally raise their sheep by releasing and allowing their livestock to graze wildly. This causes the sheep to receive feed intake with unbalanced nutrition. One of the student programs in the KKN-T IPB University in Pantai Mekar Village, Muara Gembong District, Bekasi Regency, were checking physical conditions, socializing management of sheep maintenance and distribution of vitamin B complex which to improve the health of the sheep. It was reported that four sheep were found dead after B complex vitamin administration to in four days. The sheep cadavers were brought to the School of Veterinary and Biomedical Sciences, IPB to undergo carcass surgery or necropsy to reveal the cause of death. The results of necropsy found Haemonchus contortus worm infestation in the abomasum of sheep, accompanied by accumulation of fluid under the skin, chest cavity, stomach, and lungs. The cause of death of the four sheep based on necropsy was caused by severe infestation of the Haemonchus contortus type of worm and malnutrition, which lead to hypoalbuminemia and progressed to edema in the lungs, which resulted in shortness of breath (dyspnea). In this case the necropsy results showed that the administration of vitamin B complex was not the cause of the death of the sheep.

Efek Andrographolide Oral Terhadap Biomarker Jantung pada Tikus yang Diinduksi Doxorubicin Silmi Hanifah; Wawaimuli Arozal; Vetnizah Juniantito
Journal of Medicine and Health Vol 5 No 2 (2023)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28932/jmh.v5i2.5026

Doxorubicin remains the most prescribed anticancer agent despite its unintended side effects on non-target organs. A limiting-dose strategy is used to lower incidence of cardiotoxicity. Andrographolide has therapeutic effects including antioxidant and anti-inflammatory. This study aimed to assess cardioprotective effects of andrographolide oral on lactate dehydrogenase (LDH), creatine kinase-myocardial band (CK-MB), and relative cardiac weight in doxorubicin-induced rats. Sixteen male rats Sprague Dawley randomized into four groups: receives saline i.p and vehicle orally (Normal), doxorubicin 16 mg/kgBW i.p and vehicle orally (Dox), doxorubicin 16 mg/kgBW i.p+andrographolide 30 mg/kgBW orally (Dox+And30), doxorubicin 16 mg/kgBW i.p+andrographolide 60 mg/kgBW orally (Dox+And60). Blood was collected via cardiac puncture and cardiac organs were weighed after four-weeks administration. Total LDH and CK-MB measured spectrophotometrically. LDH and CK-MB levels significantly elevated, and signs of acute toxicity in Dox group compared with Normal group. Co-treatment with andrographolide at 30 mg/kgBW and 60 mg/kgBW reduced signs of toxicity and significantly attenuated LDH and CK-MB levels compared with Dox group (P<0.05 and P<0.01). However, body weight and relative cardiac weight were not significantly different in all groups after co-treatment with andrographolide. In conclusion, andrographolide lowered LDH and CK-MB levels, therefore has a protective potency in alleviating toxic effects of doxorubicin.

MOLECULAR DETECTION OF Coxiella burnetii THE CAUSE OF ZOONOSIS Q FEVER IN VARIOUS ORGANS OF CATTLE IN BANDUNG REGENCY Tsulatsy Puspitasari Purnamiharja; Vetnizah Juniantito; Rahayu WoroWiranti; Agus Setiyono
Jurnal Kedokteran Hewan Vol 17, No 3 (2023): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i3.32716

Primary objective of this study was to ascertain the presence of Coxiella burnetii (C. burnetii) as the causative of Q fever in cattle within Bandung Regency. Tissue samples were procured from 240 slaughtered cattle’s in abattoirs across Bandung Regency. Each breed of cattle consists of Brahman cross (BX), Simmental, Limousin, Friesien Holstein crossbreed, and Friesien Holstein (FH) with total of 192, 3, 13, 29, and 3, respectively. Detection method employed was nested-Polymerase Chain Reaction (PCR) to identify the presence of C. burnetii Deoxyribonucleic Acid (DNA) within tissue samples. Positive samples underwent further examination employing hematoxylin-eosin (HE) staining to identify lesions attributed to C. burnetii. Five BX cattle were identified as positive for C. burnetii in lungs and heart. However, histopathological analysis of both organs did not reveal any notable changes. These findings suggest the occurrence of C. burnetii infection in cattle within Bandung Regency, without specific histopathological changes indicative of Q fever lesions. In conclusion, presence of asymptomatic C. burnetii infections should be regarded as a significant threat to human health.

Eksplorasi Parasit pada Ikan Nila (Oreochromis niloticus) dari Penjual Ikan Lokal di Dramaga, Bogor Azizah, Hanifah Nur; Nugraha, Arifin Budiman; Juniantito, Vetnizah; Cahyaningsih, Umi
JURNAL KAJIAN VETERINER Vol 10 No 2 (2022): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v10i2.7973

The demand for tilapia fishing goods has recently expanded on a national scale. One of the difficulties this fish farming operation encountered was the parasite-caused illness. The aims of this study were to explore parasites in tilapia (Oreochromis niloticus) from the local fish seller in Dramaga, Bogor, West Java. A total of 14 tilapia were sampled, with an average length of 22 cm and a body weight of 195 grams. Fish gills, pectoral fins, caudal fins, tail fins, dorsal fins, scales, and intestines were collected. The methods used in this study were wet mount examination, gut samples stained with Ziehl Neelsen, and blood samples from fish obtained and stained with Giemsa for analysis. The findings revealed that Trichodina sp. (85.71 percent) was present in several organs, with the gills being the most heavily affected organ. The intensity of this parasite was 27.83 individuals/head. The parasites detected by Ziehl Neelsen staining were Myxobolus sp. (57.14 %), Cryptosporidium spp. (35.71 %), and Cyclospora sp. (14.29%). The type of blood parasite found was identified as Hemogregarina sp (50%). Our findings are very important information for initiating the development of a treatment strategy to prevent parasite infection in tilapia fish farms.

Co-Authors . Jumari Abdullah Baharun Agatha Grace Agus Setiyono Agus Setyono Aisyah Aisyah Akhmad Endang Zainal Hasan Alfina Zalfa Suryono Ali Khomsan Andrea Laurentius Annisa Rahmi Antonius Anre Sianturi Armando Balses Ishimora Azizah, Hanifah Nur Baharun, Rasyid Bambang Pontjo Priosoeryanto Bayu Febram Prasetyo Cesilia M Dwiriani D R Agungpriyono Deded Sarip Nawawi Dewi R Agungpriyono Dondhin Sajuthi Dondin Sajuthi Dwi Rofifah Putri Jania Endang Syamsudin Eva Harlina Evy Ayu Arida Evy Damayanthi Fajar Rezandaru Femmi Dwinda Agustini Fivi Melva Diana, Fivi Melva Gregorius Bhaskara Wikanendra I Nyoman Jaya Wistara IETJE WIENTARSIH Iis Ismawati Indra Hadikrishna Itje Wintarsih Kusmardi Kusmardi L N Sutardi Madyastuti, Rini Makhroja, Lalu Faris Naufal Miranti Fardesiana Putri Mohamad Sadikin Mokhamad Fahrudin Monica Septiani Nafrialdi Nafrialdi Ni Wayan Kurniani Karja Nina Herlina Nopen Meisaroh Nugraha, Arifin Budiman Nur Indrawaty Lipoeto Putri Ramadhanty Rafida Nisa Maghfiroh Rahayu WoroWiranti Ranady Perwira Nuruzzaman Rimbawan , Rita Kartika Sari Safitri, Debby Endayani Sajuthi, Dondhin Sharon Aurellia Silmi Hanifah Siti Magfiroh Soni Siswanto Sri Estuningsih Sri Wahyuni Salam Sri Widia A Jusman Subangkit, Mawar Sugeng, Santoso Syahruddin Said Titta Novianti Tsulatsy Puspitasari Purnamiharja Umi Cahyaningsih Vici Eko Handayani Wahono Esthi Prasetyaningtyas Wawaimuli Arozal Wawaimuli Arozal Wawaimuli Arozal Yenny Rakhmawati

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