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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Ilmu Dasar Indonesian Journal of Cancer Chemoprevention AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Bioeksperimen: Jurnal Penelitian Biologi Pharmacon Majalah Obat Tradisional Journal of Tropical Biodiversity and Biotechnology The Indonesian Biomedical Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Biologi Tropis JSFK (Jurnal Sains Farmasi & Klinis) Menara Perkebunan Advances in Tropical Biodiversity and Environmental Sciences Proceeding Biology Education Conference Berkala Ilmiah Biologi
Tri Rini Nuringtyas
Department Of Biology, Faculty Of Biology, Universitas Gadjah Mada, Jl. Bulaksumur, Sleman, DI Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Veterinary

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Journal : Berkala Ilmiah Biologi

 1 
Cytotoxic Activity of Castanopsis argentea (Blume) A.DC. Extract and Fraction on MCF-7 Breast Cancer Cell Nuringtyas, Tri Rini; Nastiti, Anisa Diana; Surta, Muhammad Imam; Lailaty, Intani Quarta; Damayanti, Frisca
Berkala Ilmiah Biologi Vol 16 No 1 (2025)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v16i1.9851

Abstract

Marine algae is a biological source that is rich in bioactive activity. Marine algae is an important source of macronutrients, especially proteins and lipids, as well as micronutrients such as vitamins and minerals along with dietary fiber and other minoritarian constituents such as polyphenols. This study aimed to determine the antioxidant of hydrolysate protein of Sargassum sp. as. The work method was started by collecting Sargassum sp. in the intertidal zone of the South Coast of Gunungkidul, Yogyakarta and then brought to the laboratory for extraction. Protein hydrolysate was obtained by protein extraction using phosphate buffer and triRNA kit. Several buffers was used to optimize the extraction, namely PBS buffer, Tris HCl buffer and plant extraction buffer The protein obtained were evaluated in the SDS-PAGE electrophoresis. The isolated protein subjected for the trypsin digestion to obtain the hydrolysate protein then followed by antioxidant assays using DPPH test. Data analysis was carried out using ANOVA statistics for the antioxidant test. Several ratios of trypsin and protein are used to find the optimal ratio to produce protein hydrolysate. From the result it was observed that the best buffer solvent to use was Tris HCl buffer for the isolation of Sargassum sp. This can affect the protein profile of the Sargassum sp protein hydrolysate. by producing higher protein precipitates than other buffer types.
Antibacterial Activity of Leaf and Twig Extracts of Castanopsis argentea (Blume) A.DC. Against Escherichia coli and Staphylococcus aureus Amalia, Alifa; Lailaty, Intani Quarta; Surya, Muhammad Imam; Nuringtyas, Tri Rini; Damayanti, Frisca
Berkala Ilmiah Biologi Vol 16 No 1 (2025)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v16i1.19731

Abstract

Castanopsis argentea is a potential souce of natural antibiotics that has not been studied much. The purpose of this study was to analyze the antibacterial activity and study the content of secondary metabolites from leaves and twigs extract of Castanopsis argentea against Escherichia coli and Staphylococcus aureus bacteria. This study used the paper disk method to see antibacterial activity. Antibacterial activiy was indicated by the formation of a clear zone around the dic paper. Samples were made into three treatments, namely the concentration of 20 mg/ml; 40 mg/ml; and 80 mg/ml. the solvent used was selected based on the level of polarity namely ethyl acetate, 70% ethanol, and aquades. The results of the antibacterial activity test showed that the extract with the most potential to inhibit bacterial growth was ethyl acetate twig extract at a concentration of 80 mg/ml with an average diameter of 6mm for Escherichia coli bacteria and 8.67 mm for Staphylococcus aureus bacteria. The compounds identified in the ethyl acetate twig extract are terpenoids, phenolics, and flavonoids. The results of profiling using UV-Vis spectrophotometry showed that the maximum absorption of terpenoids, flavonoids, and phenolics compounds was found in the wavelength range of 300-600 nm. The secondary metabolite compounds contained in the leaf and twig extracts of Castanopsi argentea make this species a potential natural antibacterial.
Co-Authors Adita Yuniati Puspitasari Afifah, Laelatul ALIM ISNANSETYO Amalia, Alifa Andi Nur Cahyo Andyra, Vania Uly Aries Bagus Sasongko Caroline Dwiseptianti Chandra, Klara Kharisma Bunga Charlie Ester de Fretes Damayanti, Frisca Dedi Setiadi Denis FABRE Dewi, Siska Noviana Diani Mentari Dio N. Wijaya Donny Widianto Dwi Indriani Manurung Dwiseptianti, Caroline Dyah Ismoyowati, Dyah Eka Tarwaca Susila Putra Eko Pujiono Endar Marraskuranto Enik Nurlaili Afifah Erwin Nur Indiarto Fajri Marisa Febri Adi Susanto Febri Adi Susanto Harno Dwi Pranowo Hiqmah Yusi Yana ILG Nurtjahjaningsih, ILG JAKA WIDADA Krisnanda Surya Dharma Krisnanda Surya Dharma L. Hartanto Nugroho Lailaty, Intani Quarta Liliana Baskorowati Lindia Anggraini Lindia Anggraini Lisna Hidayati Marlyn Dian Laksitorini, Marlyn Dian Mashudi Mashudi Megayani Sri Rahayu Mirtani Naima Muhammad Imam Surya Muhammad Nursid Mustafid Rasyiid Naima, Mirtani Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Nastiti, Anisa Diana Novitasari, Laras Nugroho, Hartanto Nurrahmi Dewi Fajarningsih, Nurrahmi Dewi Pascal MONTORO Rahma Nurdiyanti Rahma Nurdiyanti Rahmawati, Rahmawati Rendi Mahadi Rendi Mahadi Respati Tri Swasono Rina Laksmi Hendrati Rina Sri Kasiamdari Riris Istighfari Jenie Riska Wulansari Ronny Martien Rudi Hari Murti Rudi Hari Murti Sebastian, Alfino Setiowati, Retno Diah Sri Wening Sudarsono Sugeng Pudjiono, Sugeng Sumardi . Surta, Muhammad Imam Susanto, Febri Adi Tri Joko Raharjo Tri Joko Raharjo Tri Wibawa Triyaningsih Triyaningsih, Triyaningsih Wibisono, Mochamat Gunawan Wulansari, Riska Yekti Asih Purwestri Yosi Bayu Murti Zulkifli Zulkifli