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All Journal JURNAL KIMIA SAINS DAN APLIKASI Bulletin of Chemical Reaction Engineering & Catalysis Indonesian Journal of Chemistry Indonesian Journal of Natural Pigments Journal of Multidisciplinary Applied Natural Science Makara Journal of Science Molekul: Jurnal Ilmiah Kimia Bioactivities Bulletin of Chemical Reaction Engineering & Catalysis
Kurniawan, Yehezkiel Steven
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Other

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Journal : Makara Journal of Science

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Highly Sensitive Phenol Biosensor Utilizing Selected Bacillus Biofilm Through an Electrochemical Method Ariyanti, Dita; Iswantini, Dyah; Sugita, Purwantiningsih; Nurhidayat, Novik; Effendi, Hefni; Ghozali, Ali Aulia; Kurniawan, Yehezkiel Steven
Makara Journal of Science Vol. 24, No. 1
Publisher : UI Scholars Hub

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Abstract

An eco-friendly phenol biosensor from Bacillus biofilm was prepared and investigated. The biofilm, which produced tyrosinase enzyme, was successfully immobilized on a screen-printed carbon electrode surface. A total of 72 Bacillus isolates were utilized because of their capability to produce tyrosinase enzyme in tyrosine media. Among them, Bacillus isolate code 100 was selected because it produced an adequate amount of tyrosinase enzyme and a high potentiostat current. The response surface methodology was also used to optimize the phenol sensing condition through an electrochemical method. Results showed that the optimum condition was achieved after 6 days on a phosphate buffer solution (pH of 8), with an optical density of 0.33. Furthermore, the limits of detection and quantification were 3.0 and 13 ng/L, respectively. The measurements of precision yielded a relative standard deviation of < 5%, which is remarkable. Although the biosensor material was used for 35 days, the current throughout was still maintained at 90%, indicating that the evaluated biosensor material has the potential to be used for phenol monitoring on environmental samples in the near future.
Co-Authors Abdul Karim Zulkarnain Adhi Dwi Hatmanto Adhiwibawa, Marcelinus Alfasisurya Setya Agus Dwi Ananto, Agus Agustinus Winarno Ahmad Bikharudin Alfanaar, Rokiy Amrulloh, Hanif Anggit Fitria Anggraeni, Putri Dian Anita Dwi Puspitasari Arif Cahyo Imawan Arif Cahyo Imawan Awalul Fatiqin Bambang Purwono Chairil Anwar Christyowati Primi Sagita Citrariana, Shesanthi Danny Nur Wahyu Hidayat Diah Kartika Sari Dita Ariyanti Dwi Rahmasari Fatmawati Dwi Siswanta Dyah Iswantini Edi Setiyono Ervan Yudha Ervan Yudha Eti Nurwening Sholikhah Eti Nurwening Sholikhah Eti Nurwening Sholikhah Eti Nurwening Sholikhah Fahmi, Muhammad Riza Ghulam Faris Hermawan Faris Hermawan, Faris Fatimi, Hana Anisa Fatmasari, Nela Fitria, Anggit Gerry Nugraha Ghozali, Ali Aulia Hana Anisa Fatimi Harizal Harizal Harno Dwi Pranowo Harno Dwi Pranowo Harno Dwi Pranowo Harno Dwi Pranowo Hefni Effendi Hendra Hendra Hendrik Oktendy Lintang Hidetaka Kawakita Iksen Iksen Indriana Kartini Jeffry Julianus Johan Syafri Mahathir Ahmad Joko Waluyo Jumina Jumina Jumina Jumina Jumina Jumina Jumina Jumina Jumina Jumina Jumina Jumina Kasta Gurning Keisuke Ohto Keisuke Ohto Kesuma, Ruth Febriana Kevin Thomas Krisfian Tata Aneka Priyangga Krisfian Tata Aneka Priyangga Krisfian Tata Aneka Priyangga Krisfian Tata Aneka Priyangga Krisfian Tata Aneka Priyangga Langit Cahya Adi Lathifah Puji Hastuti Leny Yuliati Limpat Nulandaya Lintang, Hendrik Oktendy Marlina, Lala Adetia Masatoshi Maeki Masaya Miyazaki Mizuki Ryu Muhammad Fernadi Lukman Muhammad Idham Darussalam Mardjan Mu’afa Purwa Arsana Nela Fatmasari Nela Fatmasari Nisa, Siti Astika Novik Nurhidayat Nursofia, Baiq Ike Philip Anggo Krisbiantoro Priastomo, Yoga Priyangga, Krisfian Tata Aneka Purnomo, Tantyo Ardy Bintoro Purwantiningsih Sugita Putra, Nicky Rahmana Rahman, Sudarman Ramachandra Rao Sathuluri Rizky Riyami Putri Rizky Riyami Putri Rokiy Alfanaar Shintaro Morisada Tantiana Indriani Thathit Suprayogi Triyono Triyono Tutik Dwi Wahyuningsih Tutik Dwi Wahyuningsih Tutik Dwi Wahyuningsih Vina Aida Roza Wataru Iwasaki Wibowo, Susalit Setya Yahya Febrianto Yudha Ramanda Yudha, Ervan