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All Journal Jurnal Pendidikan Kewarganegaraan Undiksha Media Penelitian dan Pengembangan Kesehatan Dentika Dental Journal Jurnal Komunikasi Hukum Elkawnie The Indonesian Biomedical Journal Science and Technology Indonesia Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Biota Oto Rhino Laryngologica Indonesiana Kosmik Hukum Ganesha Law Review BioEksakta : Jurnal Ilmiah Biologi Unsoed Molekul: Jurnal Ilmiah Kimia
Daniel Joko Wahyono
Fakultas Biologi, Universitas Jenderal Soedirman, Purwokerto, Jawa Tengah, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Veterinary

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Journal : Molekul: Jurnal Ilmiah Kimia

 1 
Evaluating Porins OmpK35 and OmpK36 mRNA Expression in Ciprofloxacin-Resistant Klebsiella pneumoniae Sulistiyawati, Indah; Wahyono, Daniel Joko; Siswandari, Wahyu
Molekul Vol 19 No 1 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.1.10134

Abstract

Porin messenger RNA (mRNA) expression significantly influences porin permeability and reduces antibiotic penetration. Klebsiella pneumoniae has developed resistance to several antibiotics. Decreased expression levels of OmpK35 and OmpK36 porins result in changes in the porin profile and even functional loss of porins. This research aimed to analyze the relative expression of the outer membrane porins, OmpK35 and OmpK36. The research methodology involved MIC assays and quantification of OmpK35 and OmpK36 mRNA using RT-qPCR. The relative expression of OmpK35 and OmpK36 in clinical isolate K. pneumoniae is 0.8925 and 0.5877, respectively. The porin permeability of OmpK35/K36 mRNA positively correlated with the MIC values of OmpK35 (p-value = 0.029) and OmpK36 (p-value = 0.016), respectively. Keywords: Ciprofloxacin, Klebsiella pneumoniae, OmpK35/K36, porin permeability
Regional Variations in rpoB Gene Mutations and Their Association with Rifampicin Resistance in Mycobacterium tuberculosis Widodo, Widodo; Wahyono, Daniel Joko; Oedjijono, Oedjijono; Pramono, Hendro
Molekul Vol 20 No 1 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.1.13215

Abstract

ABSTRACT. The increasing incidence of tuberculosis (TB) remains a global challenge, driven by the emergence of Mycobacterium tuberculosis resistance to antituberculosis drugs. This study investigated the relationship between rpoB gene mutations, rifampicin resistance levels, and the geographic origin of multidrug-resistant M. tuberculosis (MDR-TB) isolates. A total of 30 MDR-TB isolates were collected from the Central Java Provincial Health and Calibration Testing Laboratory between January and December 2023. Rifampicin resistance levels ranged from 5 to 80 µg/mL and were assessed using the microculture method, while DNA sequencing identified rpoB mutations. The results showed that rifampicin exposure significantly influenced rpoB mutation frequencies, with Ser531Leu (50%), His526 (16.7%), Leu511 (6%), Leu533 (6%), and Gln513 (6%) being the most common. Geographic variations were observed, with isolates from the ex-residencies Pekalongan, Pati, and Banyumas Residencies harboring seven, six, and four distinct mutations, respectively. Mutations such as Leu511Arg+His526Ser and Asp516Val were detected at low rifampicin concentrations (5–10 µg/mL), whereas Ser531Leu mutations dominated at moderate levels (20–80 µg/mL). These findings confirmed that rpoB mutations were influenced by both the region of origin and rifampicin resistance levels, providing critical insights for improving TB diagnosis and optimizing MDR-TB treatment strategies. Keywords: Mycobacterium tuberculosis, rifampicin resistance, rpoB gene mutation
Co-Authors AGUS HERY SUSANTO Ahsanal Kasasiah Andreanne Poppy Estania Anton Budhi Darmawan Arie Ardiansyah Nugraha Ashar, Fadli Bambang hermani Damara, Dandy Satria Dewi Setia Triana, Ikama Dodi Safari Dodi Safari Dody Novrial, Dody Elly Kristiani Purwendah ENDANG SRIMURNI KUSMINTARSIH ENDANG SRIMURNI KUSMINTARSIH Fitri, Safira Fuadi, Muflih Gumilas, Nur Signa Aini Haris Budi Widodo Hendro Pramono Hendro Pramono Hidayat Sulistyo Irfan Dwiandhono Jekmal Malau Korrie Salsabila Kusumaningsih, Oti Leader Alfason Lestari, Agatha Nabilla Lilis Mulyani Maretra Anindya Puspaningrum Meilani, Nanda Diva Miranti Oviani Nawangtantrini, Gita Nuniek Ina Ratnaningtyas Nur Signa Aini Gumilas Oedjijono Oedjijono, Oedjijono Oviani, Miranti Prayitno, Priskila Agnesia Priskila Agnesia Prayitno Pudyastiwi, Elisabet Purnomo Soeharso Reinhard Simbolon Rokhmani Rokhmani Rokhmani Rokhmani, Rokhmani Roni Afriansya Safari, Dodi Saryono Setianingsih, Tri Yuli Setyawati, Endang Ariyani Siwi Pramatama Mars Wijayanti Sulistiyawati, Indah Tri Yulia Ningsih Vivi Setiawaty Vivi Setiawaty Wahyu Siswandari Widodo Widodo Wisiva Tofriska Paramaiswari Zahra, Aliya Azkia Zahro, Aurora Fatimatuz