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All Journal Jurnal Ilmu Dasar Jurnal Sains dan Teknologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Kesehatan Andalas Jurnal Pendidikan Biologi Panrita Abdi - Jurnal Pengabdian pada Masyarakat Jurnal Ilmiah Universitas Batanghari Jambi Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Pengabdian Magister Pendidikan IPA Diseminasi : Jurnal Pengabdian kepada Masyarakat Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Madaniya Jurnal Abdi Masyarakat Indonesia Bioscientiae, Jurnal ilmu ilmu Biologi Jurnal Pemantik Molekul: Jurnal Ilmiah Kimia Scientific Timeline BIOTROPIC
Sulistiyawati, Indah
Program Studi Biologi, Fakultas Sains Dan Teknologi, Universitas Nahdlatul Ulama Purwokerto, Jl. Sultan Agung No. 42, Purwokerto Selatan, Banyumas, Jawa Tengah, 53144
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Public Health Social Sciences Veterinary Other

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UJI CEMARAN BAKTERI COLIFORM DAN Escherichia coli PADA AIR BERSIH SEBAGAI INDIKATOR KUALITAS LINGKUNGAN Silmi Kafah; Indah Sulistiyawati
Bioscientiae Vol 23, No 1 (2026): Bioscientiae Volume 23 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v23i1.18192

Abstract

Clean water plays a vital role in supporting human health and daily activities. This research aimed to analyze microbiological contamination specifically coliform and Escherichia coli bacteria in clean water as indicators of environmental quality. The study employed the membrane filter method to examine 20 water samples collected from various sources including wells, springs, and PDAM water. Microbiological tests followed the standard procedures set by the Ministry of Health Regulation No. 2 of 2023. In addition to microbiological testing, chemical parameters such as pH and iron content were also analyzed using a pH meter and UV-Vis spectrophotometer. The results indicated that not all samples met the required quality standards. Several samples contained coliform and E. coli levels exceeding the permissible limit of 0 CFU/100 mL, making them unsuitable for consumption. High bacterial levels were linked to poor sanitation practices around the water sources. The study also found that a few samples had elevated pH levels and iron concentrations, which may pose health risks. This research contributes valuable insights into water quality monitoring and underlines the importance of environmental hygiene in ensuring safe water sources.
SKRINING SIFILIS MENGGUNAKAN Treponema pallidum RAPID PADA PEKERJA MIGRAN INDONESIA DI KABUPATEN BANYUMAS Fadhil Rabbani; Indah Sulistiyawati
Bioscientiae Vol 23, No 1 (2026): Bioscientiae Volume 23 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v23i1.18190

Abstract

Syphilis is a sexually transmitted infection (STI) caused by Treponema pallidum subspecies pallidum that continues to pose a significant public health challenge, particularly among groups such as Indonesian migrant workers (PMI). Early detection is essential in preventing transmission and ensuring appropriate treatment. This study aimed to determine the prevalence of syphilis among Indonesian migrant workers using the Treponema pallidum Rapid Test (TP-Rapid) at a health facility in Banyumas Regency. The diagnostic procedure was conducted in three stages such as pre-analytic, analytic, and post-analytic. A quantitative descriptive method with a cross-sectional approach was employed involving 160 Indonesian migrant workers undergoing pre-departure health screening. Blood samples were collected and tested using the TP-Rapid method, which offers rapid, sensitive, and specific detection of T. pallidum antibodies. Results indicated that 10 out of 160 samples (6,25%) was reactive for syphilis, while 150 samples (93,75%) were non-reactive. Despite the relatively low prevalence, the findings underscore the necessity of routine syphilis screening. The TP-Rapid test was found to be a practical diagnostic tool due to its simplicity, rapid turnaround time, and reliability in identifying syphilis seroreactivity. These results highlight the importance of integrating early syphilis detection into mandatory health screenings for migrant workers to mitigate potential transmission and safeguard both individual and public health.
DETEKSI HEPATITIS B Surface Antigen (HBsAg) DENGAN PERBANDINGAN WAKTU DAN AKURASI RAPID TEST DAN Enzyme-Linked Immunosorbent Assay (ELISA) Nasywa Luthfi Zain; Indah Sulistiyawati; Muryanti Muryanti
Bioscientiae Vol 23, No 1 (2026): Bioscientiae Volume 23 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v23i1.18191

Abstract

Hepatitis B is one of the serious infectious diseases that is still a global health problem. Early detection of HBsAg infection is very important to prevent further complications. This study aims to compare the detection time and accuracy between the rapid test and Enzyme-Linked Immunosorbent Assay (ELISA) in detecting HBsAg. The study used two methods, then analyzed based on age and gender variables. The rapid test showed advantages in detection speed, but had limitations in accuracy. Meanwhile, ELISA takes longer but provides more sensitive and specific results. The results of this study indicate that the selection of diagnostic methods must consider clinical needs so that the combination of both methods can increase the effectiveness of early detection and control of Hepatitis B infection in the community.
PEMERIKSAAN BAKTERI COLIFORM PADA SAMPEL DEPOT AIR MINUM ISI ULANG (DAMIU) Teguh Azizah; Indah Sulistiyawati
Bioscientiae Vol 23, No 1 (2026): Bioscientiae Volume 23 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v23i1.15249

Abstract

Water is one of the basic human needs, because humans are very dependent on air for both drinking and daily life. Air quality that includes is very important to ensure safe drinking water, but it is also important to consider the broader impacts of air management practices. Refill Drinking Water Depot (DAMIU). This study aims to ensure that drinking water is safe and suitable for consumption. This study was to examine coliform bacteria in drinking water samples from Refill Drinking Water Depot (DAMIU) using the membrane filter method, with 10 samples. This study uses the Membrane filter method, which is a technique used to test water samples to identify the presence of coliform bacteria and Escherichia coli. The results of testing 10 drinking water samples can be concluded that some of the quality of drinking water at the research location is still different and has not fully met the quality standards. The results are not in accordance with the maximum limit of coliform and E. coli in drinking water is 0 CFU / 100 mL in the drinking water quality standards set by the Regulation of the Minister of Health of the Republic of Indonesia (PERMENKES) Number 2 of 2023. The influencing factors are raw materials, length of time the water is stored in the reservoir, handling of the buyer's container, operator cleanliness, and environmental cleanliness around the DAMIU which is less noticed.
Co-Authors Aditya Trinanda Utama Adityo Nugroho, Adityo Agustiani, Rina Dwi Andika Ristiono Anggraeni, Gita Aniuranti, Asfi Ari Dwi Nurasih Chamid Sutikno Daniel Joko Wahyono Denny Indra Yudhistira Diah Arimbi Diah Harmawati Eko Hendarto Eko Hendarto Eti Wahyuningsih Fadhil Rabbani Falah, Muhammad Fitria Septiana Purwitaningrum Gita Anggraeni Hendro Pramono Herdian Farisi I Gede Arinton Indah Dwi Prigitaningtias Karseno Khoiriyah, Anna Jarrotul Maditaningtyas Hawwa Zuwanda Mimien Heni Irawati Al Muhdhar, Mimien Heni Irawati Muhammad Falah Muryanti Muryanti Nasywa Luthfi Zain Neli Oktaviani Nur Laila Rahayu Nur Laila Rahayu Nura Khabita Nuraini Muhassanah Nurasih, Ari Dwi Oksa Hugi Lestiani Rahayu, Nur Laila Rahayu, Nurlaila Rina Dwi Agustiani Rovik, Anwar Sadiah, Hilmi Halimatus Silmi Kafah Siti Fatimah Teguh Azizah Tien Setyaningtyas Wahyu Maulana Endris Wahyu Siswandari Zainul Mustofa, Zainul Zulaikhah, Siti Rahmawati