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All Journal Jurnal Ilmu Dasar Jurnal Sains dan Teknologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Kesehatan Andalas Jurnal Pendidikan Biologi Panrita Abdi - Jurnal Pengabdian pada Masyarakat Jurnal Ilmiah Universitas Batanghari Jambi Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Pengabdian Magister Pendidikan IPA Diseminasi : Jurnal Pengabdian kepada Masyarakat Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Madaniya Jurnal Abdi Masyarakat Indonesia Bioscientiae, Jurnal ilmu ilmu Biologi Jurnal Pemantik Molekul: Jurnal Ilmiah Kimia Scientific Timeline BIOTROPIC
Sulistiyawati, Indah
Program Studi Biologi, Fakultas Sains Dan Teknologi, Universitas Nahdlatul Ulama Purwokerto, Jl. Sultan Agung No. 42, Purwokerto Selatan, Banyumas, Jawa Tengah, 53144
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Evaluating Porins OmpK35 and OmpK36 mRNA Expression in Ciprofloxacin-Resistant Klebsiella pneumoniae Sulistiyawati, Indah; Wahyono, Daniel Joko; Siswandari, Wahyu
Molekul Vol 19 No 1 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.1.10134

Abstract

Porin messenger RNA (mRNA) expression significantly influences porin permeability and reduces antibiotic penetration. Klebsiella pneumoniae has developed resistance to several antibiotics. Decreased expression levels of OmpK35 and OmpK36 porins result in changes in the porin profile and even functional loss of porins. This research aimed to analyze the relative expression of the outer membrane porins, OmpK35 and OmpK36. The research methodology involved MIC assays and quantification of OmpK35 and OmpK36 mRNA using RT-qPCR. The relative expression of OmpK35 and OmpK36 in clinical isolate K. pneumoniae is 0.8925 and 0.5877, respectively. The porin permeability of OmpK35/K36 mRNA positively correlated with the MIC values of OmpK35 (p-value = 0.029) and OmpK36 (p-value = 0.016), respectively. Keywords: Ciprofloxacin, Klebsiella pneumoniae, OmpK35/K36, porin permeability
PENERAPAN STRATEGI PEMBELAJARAN PROBLEM BASED LEARNING MELALUI LESSON STUDY UNTUK MENINGKATKAN KETERAMPILAN MEMECAHKAN MASALAH MAHASISWA Mustofa, Zainul; Khoiriyah, Anna Jarrotul; Sulistiyawati, Indah; Harmawati, Diah; Al Muhdhar, Mimien Heni Irawati
Jurnal Pendidikan Biologi Vol 8, No 1 (2016)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.75 KB) | DOI: 10.17977/um052v8i1p32-37

Abstract

This research aims at improving students' problem solving skills. The type of research used is Classroom Action Research by implementing Lesson Study-based Problem Based Learning strategy as an effort to improve students’ problem solving skills. This study was conducted for 1 cycle consisting of two meetings. The results of this research show that LS-based PBL strategy can improve students' problem solving skills, from 30% during observation to 93%. The data of students' average problem solving skill scores reached 80.07. The achievement of this score has met the minimum standard score of State University of Malang, which is 55.00. Based on these results, learning by using LS-based PBL strategy can improve students' problem solving skills.
Efficacy of Ethanol Extract of Sambiloto (Andrographis paniculata) Against Pneumonia-Causing Klebsiella pneumoniae Sulistiyawati, Indah; Falah, Muhammad; Nurasih, Ari Dwi
Jurnal ILMU DASAR Vol 26 No 1 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i1.36846

Abstract

Klebsiella pneumoniae is one of the most common pathogens in nosocomial respiratory infections. Antibiotics are often used for the treatment of K. pneumoniae infections. Increased bacterial resistance to antibiotics is closely related to the success rate of infectious disease therapy. The use of plants as phytopharmacology is currently an alternative treatment. Some medicinal plants that have antibacterial and immunomodulatory activity are Sambiloto (Andrographis paniculata) with an active compound called andrographol. The purpose of this study was to provide a solution to the problem above by developing a phytopharmaceutical using bitter extract to inhibit the growth of K. pneumoniae bacteria. Two sputum samples from the laboratory confirmed Covid-19 patients with pneumonia infection at the Hospital. The sambiloto was extracted by using 96% ethanol. The extract was further analyzed for its antimicrobial activity and phytochemical compounds. There were two K. pneumoniae isolates, namely KpRNG and KpTRI. The active compounds were saponin and steroids. The antimicrobial activity of the ginger extract was measured with different concentrations of 25%, 50%, 75%, and 100%. Its inhibitory zone against K. pneumoniae was ranging from 5.8 to 7 mm or had a moderate inhibition category.
PROFIL M. tuberculosis PASIEN TB SENSITIF RIFAMPISIN TERHADAP LAMA WAKTU PENUNDAAN PEMERIKSAAN SPUTUM Sulistiyawati, Indah; Fatimah, Siti; Rahayu, Nur Laila
Bioscientiae Vol 22, No 1 (2025): Bioscientiae Volume 22 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v22i1.14676

Abstract

Tuberculosis (TB) is an infectious disease that is currently still a serious health problem in society, both in terms of mortality rates, morbidity rates, diagnosis and its therapy rates. Sputum quality is an important component in laboratory examinations. The quality of sputum samples can affect the number of M. tuberculosis bacteria. This study aims to determine the differences in the morphological characteristics of M. tuberculosis bacteria with the length of time of delay in sputum sample examination. The study was conducted at the Klinik Utama Kesehatan Paru Masyarakat Purwokerto. The study used a descriptive method with a cross-sectional design. The sample population of this study were all suspected TB patients who underwent Molecular Rapid Technique (MRT) examinations at the Klinik Utama Kesehatan Paru Masyarakat Purwokerto (KKPM) in the January-June 2024. The research sampling technique was purposive sampling that met the inclusion criteria. The results of the study showed that the morphological characteristics of the M. tuberculosis bacteria vary in each sputum sample, based on differences in the quality of the sputum examined with a general description of the morphological characteristics of the bacteria, which have the form of monobacillus, diplobacillus and bacillus, solitary and in colonies, and are a type of Gram-positive bacteria.  Keywords: Mycobacterium tuberculosis, MDR-TB. TCM test, GeneXpert 
ANALISIS MIKROBILOGI COLIFORM PADA PRODUK INDUSTRI RUMAH TANGGA SIOMAY DI KECAMATAN PURWOKERTO SELATAN Sulistiyawati, Indah; Sadiah, Hilmi Halimatus; Rahayu, Nurlaila
Bioscientiae Vol 22, No 1 (2025): Bioscientiae Volume 22 No 1
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/b.v22i1.14614

Abstract

Jajanan siomay merupakan salah satu makanan yang banyak diminati oleh masyarakat dan dijual bebas di pasar tradisional. Bahan makanan mudah tercemar oleh golongan bakteri Coliform adalah bahan makanan tradisional dengan pengolahan sederhana. Jajanan siomay rentan terkontaminasi oleh berbagai bakteri patogen akibat kurangnya penerapan higiene dan sanitasi penjamah makanan. Penelitian ini mengidentifikasi keberadaan bakteri Colifom pada produk industri rumah tangga siomay di Kecamatan Purwokerto Selatan. Penelitian ini merupakan penelitian observasional laboratorik dengan teknik standar pemeriksaan mikrobiologi makanan dan minuman menggunakan metode kuantitatif yaitu Most Probable Number (MPN) dan Membran Filter. Teknik pengambilan sampel penelitian adalah purposive random sampling. Variabel yang diamati adalah analisis bakteri Coliform pada siomay, air yang digunakan dalam proses pembuatan siomay dan higiene sanitasi tempat pembuatan siomay dengan melakukan wawancara pengisian kusioner di Kecamatan Purwokerto Selatan. Hasil yang diperoleh menunjukkan 8 dari 12 sampel siomay terkonfirmasi mengalami kontaminasi golongan bakteri Coliform. Pemeriksaan air PDAM yang digunakan dalam pengolahan siomay didapatkan hasil 0/100 mL yaitu memenuhi syarat berdasarkan Peraturan Menteri Kesehatan Nomor 2 Tahun 2023. Higiene sanitasi penjamah makanan menunjukkan bahwa pengetahuan dan sikap penjamah makanan pada pedagang siomay di kelurahan Berkoh dan di kelurahan Purwokerto kidul dari rekapitulasi rata-rata memiliki kategori pengetahuan dan sikap baik.
Profil Ekspresi MikroRNA Kanker Payudara di Purwokerto Nurasih, Ari Dwi; Sulistiyawati, Indah; Falah, Muhammad
Jurnal Kesehatan Andalas Vol. 9 No. 4 (2020): Online December 2020
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v9i4.1553

Abstract

Penemuan metode deteksi dini kanker payudara secara sensitif, spesifik dan minimal invasif merupakan kunci dalam keberhasilan pengobatan. Hal ini dapat dilakukan menggunakan biomarker yang bersirkulasi dalam plasma darah yaitu mikroRNA. MikroRNA adalah asam ribonukleat yang tidak mengkode protein tetapi memiliki peran dalam menghambat dan mendegradasi mRNA sehingga mempengaruhi perkembangan kanker. Tujuan: Mengetahui profil ekspresi mikroRNA pada pasien kanker payudara di Purwokerto dan mikroRNA yang mengalami disregulasi sebagai kandidat biomarker kanker payudara. Metode: Analisis profil ekspresi mikroRNA pada kanker payudara dilakukan dengan isolasi plasma dari pasien kanker payudara dan individu sehat, sintesis cDNA, dan kuantifikasi mikroRNA dengan quantitative real time PCR (qPCR). Kemudian dilakukan penghitungan dengan metode Livak untuk mengetahui mikroRNA yang mengalami peningkatan dan penurunan ekspresi yang signifikan. Hasil:  Biomarker kanker payudara di Purwokerto adalah MikroRNA yang mengalami upregulation adalah hsa-miR-543 (sebanyak 1612,28 kali), hsa-miR-495-3p (1797,95 kali), hsa-miR-382-5p (8780,32 kali), hsa-miR-155-5p (8552,18 kali) dan hsa-miR-154-5p (2339,41 kali). mikroRNA yang mengalami downregulation adalah hsa-miR-590-5p (sebanyak 4,70 kali), hsa-miR-33a-5p (sebanyak 74,29 kali), hsa-miR-100-5p (sebanyak 23,09 kali), hsa-miR-19b-3p (sebanyak 11,71 kali) dan hsa-miR-144-3p (sebanyak 25,30 kali) (p < 0,05). Simpulan: Biomarker yang dapat digunakan sebagai alat deteksi dan diagnostik dini kanker payudara di Purwokerto adalah miR-382-5p.Kata kunci: biomarker, kanker payudara, mikroRNA, qPCR
Inovasi teknologi modern pengolahan sirup buah mangrove solusi strategis mewujudkan kemandirian ekonomi masyarakat pesisir Sulistiyawati, Indah; Farisi, Herdian; Karseno
Jurnal Pembelajaran Pemberdayaan Masyarakat (JP2M) Vol. 6 No. 4 (2025)
Publisher : Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jp2m.v6i4.24173

Abstract

Program pemberdayaan kemitraan masyarakat ini dilaksanakan di Desa Kutawaru, Segara Anakan, Cilacap dengan tujuan meningkatkan kapasitas dan kemandirian ekonomi masyarakat pesisir melalui inovasi teknologi pengolahan buah mangrove menjadi produk pangan bernilai tambah. Metode yang digunakan adalah pendekatan partisipatif (Participatory Action Research), meliputi wawancara awal, focus group discussion (FGD), pelatihan teknis berbasis partisipasi, serta pendampingan manajemen usaha yang disertai monitoring dan evaluasi. Kebaruan program terletak pada penerapan teknologi modern untuk mengolah buah mangrove menjadi minuman sirup berkualitas, disertai strategi diferensiasi melalui branding digital dan pemasaran daring. Hasil kegiatan menunjukkan peningkatan keterampilan produksi dan pengetahuan masyarakat sebesar 21,33%, terbentuknya diversifikasi produk olahan berbasis mangrove, tersusunnya sistem manajemen usaha sederhana, serta peningkatan akses pasar melalui platform digital. Capaian ini tidak hanya meningkatkan kapasitas individu, tetapi juga memperkuat jejaring ekonomi lokal dengan potensi peningkatan pendapatan dan keberlanjutan usaha. Dengan demikian, tujuan program untuk memberdayakan masyarakat pesisir berbasis inovasi teknologi dan strategi pemasaran modern dinyatakan tercapai, sekaligus menawarkan model pengabdian yang aplikatif dan replikatif bagi kawasan pesisir lainnya.
Antimicrobial Activity of Basil (Ocimum basilicum L.) Against Gram-Negative Bacteria Involved in Pneumonia Infection Sulistiyawati, Indah; Falah, Muhammad; Anggraeni, Gita; Rovik, Anwar
JST (Jurnal Sains dan Teknologi) Vol. 12 No. 2 (2023): July
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jstundiksha.v12i2.53577

Abstract

Infectious diseases are still a global problem, for example, acute respiratory infections (SARI). Resistance in pneumonia involving Gram-negative bacterial infection reduces the effectiveness of antibiotic use and increases the length of hospital stay. This study examines the potential of antimicrobial compounds from basil plants against isolates of Gram-negative bacteria involved in pneumonia infection. This research is an experimental study through several stages: basil extraction, initial screening of bioactive compounds, isolation and identification of bacteria from sputum, and testing for antimicrobial activity of basil extracts. Sputum samples were collected from 43 pneumonia patients (male and female) involving Klebsiella pneumoniae, Acinetobacter baumannii, Enterobacter aerogenes, Pseudomonas aeruginosa, Serratia marcescens, Proteus mirabilis, and Escherichia coli. The inhibitory activity against pathogenic bacteria was directly proportional to the basil extract concentration. Initial screening for phytochemical compounds showed that the extracts of basil leaves and stems contained active compounds of tannins and flavonoids. The 25% concentration of basil extract was the minimum inhibitory concentration (MIC) against the test bacteria. The 100% basil extract concentration produced the largest inhibition zone i.e., 10.93 mm. The overall findings of this study provide baseline information for the possible use of the Ocimum basilicum extract in the treatment of pneumonia involving Gram-negative bacteria.
Upaya Meningkatkan Pendapatan Kelompok Ternak Lembu Rizki Melalui Branding dan Modernisasi Pengolahan Susu Pada Program Pemberdayaan Kemitraan Masyarakat Zulaikhah, Siti Rahmawati; Sulistiyawati, Indah; Nugroho, Adityo
Jurnal Pengabdian Magister Pendidikan IPA Vol 6 No 4 (2023): Oktober-Desember 2023
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v6i4.5509

Abstract

The Lembu Rizki Livestock Group is in Singasari Village, Karanglewas District, Banyumas Regency. The problems in this group are a) Low selling price of milk in cooperatives, b) Farmers do not yet have milk processing technology, c) Limited human resources, resulting in difficulties in production and marketing. The solutions offered are 1) Sales in the form of processed milk, 2) Transfer of diversification of milk processing technology in the form of pasteurized milk of various flavors and yoghurt, 3) Empowerment of family members, and marketing using digital marketing. The general objective of this Community Partnership Empowerment activity is a) to provide solutions based on situation analysis according to the needs, challenges or problems that exist in the Lembu Rizki livestock group, b) to empower the Lembu Rizki livestock group to improve their standard of living both in economic, social and cultural terms, c) transfer technology, science and art for the development of livestock groups. The results obtained from this activity are increased target knowledge and skills regarding processing milk into pasteurized milk of various flavors and yoghurt, knowing the importance of packaging and labeling for product branding, and digital marketing. Keyword: Branding, Modernization, Milk Processing, Empowerment of Community Partnerships, Livestock Groups
Uji Ekstrak Bawang Putih Tunggal (Allium sativum L.) Terhadap Bakteriuria Escherichia coli Pada Calon Pekerja Migran Indonesia Indah Sulistiyawati; Muhammad Falah; Ari Dwi Nurasih
Biotropic : The Journal of Tropical Biology Vol. 5 No. 1 (2021): Biotropic, Volume 5 Nomor 1, 2021
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2021.5.1.50-57

Abstract

Garlic (Allium sativum L) is a tested type of phytopharmaca because it is proven to have beneficial benefits as antibacterial and protect the body from pathogens. The majority of Indonesian Migrant Workers, especially Prospective Indonesian Migrant Workers (CPMI), are indicated to be susceptible to Urinary Tract Infection (UTI), which is caused by the bacteriuria Escherichia coli. In this study, the crude extract of single garlic (A. sativum L) will be used as an antibacterial against E. coli bateriuria in the urine sample of CPMI, with variations in the concentration of single crude extract of garlic, namely; 0%, 25%, 50%, 75%, and 100%. This research was conducted in a period of 1 (one) year from January to December 2020, with urine research samples from CPMI in the Banyumas Regency area. Extraction was carried out by extracting a single garlic using 96% ethanol solvent in a ratio of 1: 1, then evaporated to produce a filtrate that would be tested with a varied concentrate composition. Isolation of bacteriuria in urine samples using EMBA selective media. From a total of 37 urine samples, 17 about 46% were positive for E. coli, and 20 samples were 54% negative for E. coli. Bacteriuria that was found challenged with crude garlic extract resulted in differences in the antibacterial test at several extract concentrations with amoxicillin positive control, with One Way Anova a P-value = 0.000 <0.05, and continued with the analysis of the Post Hoc Test and the Tuckey Test, that the garlic extract The single most effective used as an anti-bacterial agent is the concentration of 100%, resulting in an average inhibition of 12.81 mm. The ability of single garlic extract to inhibit increases with increasing extract concentrate.
Co-Authors Aditya Trinanda Utama Adityo Nugroho, Adityo Agustiani, Rina Dwi Andika Ristiono Anggraeni, Gita Aniuranti, Asfi Ari Dwi Nurasih Chamid Sutikno Daniel Joko Wahyono Denny Indra Yudhistira Diah Arimbi Diah Harmawati Eko Hendarto Eko Hendarto Eti Wahyuningsih Fadhil Rabbani Falah, Muhammad Fitria Septiana Purwitaningrum Gita Anggraeni Hendro Pramono Herdian Farisi I Gede Arinton Indah Dwi Prigitaningtias Karseno Khoiriyah, Anna Jarrotul Maditaningtyas Hawwa Zuwanda Mimien Heni Irawati Al Muhdhar, Mimien Heni Irawati Muhammad Falah Muryanti Muryanti Nasywa Luthfi Zain Neli Oktaviani Nur Laila Rahayu Nur Laila Rahayu Nura Khabita Nuraini Muhassanah Nurasih, Ari Dwi Oksa Hugi Lestiani Rahayu, Nur Laila Rahayu, Nurlaila Rina Dwi Agustiani Rovik, Anwar Sadiah, Hilmi Halimatus Silmi Kafah Siti Fatimah Teguh Azizah Tien Setyaningtyas Wahyu Maulana Endris Wahyu Siswandari Zainul Mustofa, Zainul Zulaikhah, Siti Rahmawati