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All Journal JBIO: jurnal biosains (the journal of biosciences) JURNAL PENELITIAN SAINTIKA JURNAL PENGABDIAN KEPADA MASYARAKAT BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) AMALIAH: JURNAL PENGABDIAN KEPADA MASYARAKAT Journal of Natural Sciences Jurnal Ekonomi Pertanian dan Agribisnis Biosainsdik: Biologi Sains dan Kependidikan
Pulungan, Ahmad Shafwan S
Universitas Negeri Medan
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences Other

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TESTING THE ACTIVITY OF KETUL LEAF (Bidens pilosa L.) ETHANOL EXTRACT IN INHIBITING THE GROWTH OF Escherihia coli BACTERIA Munthe, Melia Ivana; Nasution, Mhd Yusuf; Pulungan, Ahmad Shafwan S
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.52933

Abstract

Daun ketul (Bidens pilosa L.) memiliki senyawa metabolit sekunder yang dimanfaatkan masyarakat dalam mengobati penyakit diare. Daun ketul memiliki senyawa metabolit sekunder antara lain terpenoid, alkaloid, flavonoid, dan fenool yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol daun ketul (B. pilosa L.) dalam menghambat pertumbuhan bakteri Escherichia coli. Penelitian ini menggunakan metode difusi cakram yang terdiri dari 5 variasi konsentrasi yaitu 30%, 40%,50%,60%,70%, dengan 4 kali pengulangan. Data hasil zona hambat  dianalisis dengan uji statistik One Way Anova.  Hasil penelitian menunjukkan ekstrak daun ketul (Bidens pilosa L.) memiliki aktivitas antibakteri  terhadap bakteri Escherichia coli. Semakin luas zona bening yang terbentuk maka semakin kuat senyawa aktif dalam menghambat pertumbuhan bakteri. Berdasarkan hasil uji lanjutan Duncan diperoleh bahwa tidak ada perbedaan secara nyata dari setiap perlakuan konsentrasi dalam menghambat bakteri Escherichia coli.
TESTING THE ANTIBACTERIAL ACTIVITY OF MORINGA LEAF (Moringa oleifera L.) ETHANOL EXTRACT ON THE GROWTH OF THE BACTERIA Propionibacterium acnes IN VITRO Nisrina Afiqah Rahmi; Ahmad Shafwan S. Pulungan
Jurnal Biologi Sains dan Kependidikan Vol 4, No 2 (2024): BIOSAINSDIK : Jurnal Biologi Sains dan Kependidikan
Publisher : Prodi Tadris Biologi Fakultas Agama Islam Universitas Muhammadiyah Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37598/biosainsdik.v4i2.2260

Abstract

ABSTRACTThis study aims to determine the antibacterial activity of ethanol extract of Moringa oleifera L. leaves against the growth of Propionibacterium acnes bacteria in vitro and to determine the active compounds contained in the ethanol extract of Moringa oleifera L. leaves. This research is experimental and the method used is disc diffusion, namely by observing, measuring, calculating and analyzing the diameter of the growth inhibition zone of P. acnes which forms around the disc containing ethanol extract of Moringa leaves. The results of antibacterial activity show that the ethanol extract of Moringa oleifera L. leaves has antibacterial activity against the growth of Propionibacterium acnes bacteria and the active compounds contained in the ethanol extract of Moringa oleifera L. leaves which were tested using the phytochemical screening method, namely tannin and saponin compounds, and flavonoids.Keywords: Ethanol, Moringa leaves, antibacterial, Propionibacterium acnes. 
Pengaruh Pemberian Fungi Mikoriza Pada Tanaman Kangkung (Ipomea Reptans Poir.) Sitorus, Rumiris Fanessa; Anggraeni, Atika Puspita; Pulungan, M Farhan; Pulungan, Ahmad Shafwan S; Sarie, Marlinda Nilan
Jurnal Ekonomi Pertanian dan Agribisnis Vol. 1 No. 1 (2023): Juli - Desember
Publisher : CV.ITTC INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62379/jepag.v1i1.1307

Abstract

This research aims to determine the dose of mycorrhiza to increase the growth and production of water spinach plants (Ipomea reptans poir). The parameters observed were plant height and number of leaves. The research method used was RAL (Completely Randomized Design), by administering 5 treatments with doses (2.5 grams, 4 grams, 6 grams, 9 grams) and one control treatment. And each treatment with 3 repetitions. The plant height was 80 cm and the largest number of leaves was 36 in the P3 treatment (giving 6 grams of mycorrhiza). Giving a dose of mycorrhiza affects the growth of water spinach (Ipomea reptans poir).
CHARACTERIZATION OF BACTERIA Salmonella sp. ON BROILER CHICKEN MEAT MARKETED AT SUKARAMAI MARKET, MEDAN CITY Rangkuti, Marlinda Nilan Sari; Pulungan, Ahmad Shafwan S; Silaban, Friend
JURNAL BIOSAINS Vol. 9 No. 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.49964

Abstract

One effort to meet high protein needs is obtained from chicken meat. Chicken meat is a food source that is composed of various nutritional values in balanced proportions. There is still doubt about Boiler Chicken meat being sold for sale because it is suspected that there is contamination with pathogenic microbes that can cause disease in humans. There is a risk that broiler chicken meat will be contaminated due to poor market sanitation. The aim of this research was to determine the bacterial contamination of Salmonella sp. in broiler chicken meat and the number of Salmonella sp bacteria. Which is found in samples of broiler chicken meat marketed at the Sukaramai Market, Medan City. This research method is a quantitative descriptive approach. This research was carried out at the Regional Health Laboratory of North Sumatra Province in May-June 2023. In this study, sample culture was carried out by streaking on Salmonella Shigella Agar (SSA) media, while the Total Plate Count (TPC) test for Salmonella sp. using pour plate media. The results showed that all 9 samples contained Salmonella sp. with the amount of bacterial contamination, namely A1 3.0 × 10-4, A2 3.9 × 10-4, A3 4.7 × 10-4, B1 4.0 × 10-4, B2 5.3 × 10-4, B3 6.0×10-4, C1 4.5×10-4, C2 6.8×10-4, C3 7.7×10-4. Based on the National Chicken Meat Standardization Agency ICS No. 67-120-20. In 2009, the maximum limit for microbial contamination and the maximum limit for residues in food ingredients of animal origin must not contain Salmonella sp.
FIG ENDOPHYTIC BACTERIA PRODUCE ENZYMES AMYLASE, LIPASE, PROOTEASE, CELLULASE Habsari, Lilis; Gultom, Endang Sulistyarini; Nasution, Mhd Yusuf; Rangkuti, Marlinda Nilan Sari; Pulungan, Ahmad Shafwan S
JURNAL BIOSAINS Vol. 9 No. 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.52820

Abstract

The use of enzymes in Indonesia is increasing every year, so action is needed to harmonize the production and needs of enzymes in Indonesia. Enzymes can be produced from various sources such as plants, animals and microorganisms. The advantage of utilizing microorganisms in enzyme production is that they can be produced in large quantities with a shorter time and can be produced continuously. One of the sources of bacterial screening to produce enzymes is figs. This study aims to look at the extracellular enzyme activity of fig endophytic bacteria and identify endophytic bacteria capable of producing extracellular enzymes such as proteases, amylase, cellulase and lipase. Isolation and purification of bacteria using NA media for 24 hours. Test of extracellular enzyme activity using NA media enriched with amylum, tween 80, skim milk, CMC. Biochemical tests are performed to identify endophytic bacteria of figs producing extracellular enzymes. The results of the study were 21 isolates of fig endophytic bacteria, as many as 14 isolates were able to produce extracellular enzymes. The identification results of fig endophytic bacteria producing extracellular enzymes obtained 8 isolates suspected to be from the genus Bacillus, 3 isolates suspected to be from the genus Micrococcus, 1 isolate suspected to be from the genus Cellulomonas, 2 isolates suspected to be from the genus Acetobacter
TESTING THE ACTIVITY OF KETUL LEAF (Bidens pilosa L.) ETHANOL EXTRACT IN INHIBITING THE GROWTH OF Escherihia coli BACTERIA Munthe, Melia Ivana; Nasution, Mhd Yusuf; Pulungan, Ahmad Shafwan S
JURNAL BIOSAINS Vol. 10 No. 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.52933

Abstract

Daun ketul (Bidens pilosa L.) memiliki senyawa metabolit sekunder yang dimanfaatkan masyarakat dalam mengobati penyakit diare. Daun ketul memiliki senyawa metabolit sekunder antara lain terpenoid, alkaloid, flavonoid, dan fenool yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol daun ketul (B. pilosa L.) dalam menghambat pertumbuhan bakteri Escherichia coli. Penelitian ini menggunakan metode difusi cakram yang terdiri dari 5 variasi konsentrasi yaitu 30%, 40%,50%,60%,70%, dengan 4 kali pengulangan. Data hasil zona hambat  dianalisis dengan uji statistik One Way Anova.  Hasil penelitian menunjukkan ekstrak daun ketul (Bidens pilosa L.) memiliki aktivitas antibakteri  terhadap bakteri Escherichia coli. Semakin luas zona bening yang terbentuk maka semakin kuat senyawa aktif dalam menghambat pertumbuhan bakteri. Berdasarkan hasil uji lanjutan Duncan diperoleh bahwa tidak ada perbedaan secara nyata dari setiap perlakuan konsentrasi dalam menghambat bakteri Escherichia coli.
Analysis of Secondary Metbaolite Content of Ethanol Extract of Biden Pilosa L. Leaves which has the Potential as an Antibacterial Pulungan, Ahmad Shafwan S; Nasution, Aswarina; Puspitasari, Wina Dyah; Gultom, Rahmad H; Rahmi, Nisrina Afiqah; Munthe, Melia Ivana Br
JURNAL BIOSAINS Vol. 10 No. 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.62621

Abstract

The potential of plants as an alternative medicine is a great gift. This potential can be explored through various preliminary tests. One of the preliminary tests is to analyze the content of secondary metabolite compounds using GC-MS. One of the plants known to have potential is Biden Pilosa L. The results of the GC-MS analysis showed that there were 9 compounds identified as having antimicrobial properties. This was discovered after analyzing the compound content and continuing with a literature review. The conclusion obtained was that there were 9 compounds in the ethanol extract of Biden Pilosa L leaves that functioned as antimicrobials.
Co-Authors Ade Yesi Yuliana Adi Hartono Agnes Adela Br Ginting Anggraeni, Atika Puspita Ani Sutiani Ashar Hasairin Bakti Dwi Waluyo Brata, Wasis Wuyung Wisnu Diana Erawaty Tumangger Fitri Chairani Fitri Chairani Gultom, Endang Sulistyarini Gultom, Endang Sulistyarini Gultom, Rahmad H Habsari, Lilis HANDAYANI, DINA Harahap, Armansyah Maulana Marlinda Nilan Sari Rangkuti Mhd Yusuf Nasution Muhammad Yusuf Nasution Munthe, Melia Ivana Munthe, Melia Ivana Br Nanda Pratiwi Nasution, Aswarina Nasution, Mhd Yusuf Nisrina Afiqah Rahmi Pasar Maulim Silitonga Pulungan, M Farhan Puspitasari, Wina Dyah Rahmad Gultom Rahmad Gultom Rahmi, Nisrina Afiqah Rangkuti, Marlinda Nilan Sari Restuati, Martina Sarie, Marlinda Nilan Silaban, Friend Sitorus, Rumiris Fanessa Syahputra, Ricky Andi Tessa Togatorop Tumangger, Diana Erawaty Uswatun Hasanah Wulandari Wulandari Wulandari Wulandari