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Gambaran Fiksasi Jaringan Menggunakan Larutan Bouin dan NBF 10% Pada Hati Mencit Dengan Durasi Fiksasi 4 dan 16 Jam Menggunakan Pewarnaan Hematoksilin-Eosin (HE) Ignasius Kalos; Erni Yohani Mahtuti; Faisal Faisal
Jurnal Ilmu Kedokteran dan Kesehatan Indonesia Vol. 6 No. 1 (2026): Maret : Jurnal Ilmu Kedokteran dan Kesehatan Indonesia
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jikki.v6i1.8875

Abstract

Histological tissue processing remains the “gold standard” for determining patient therapy and prognosis. One of the stages of tissue processing is fixation. The types of fixative solutions used are 10% NBF and Bouin. The purpose of this study was to determine the histological features of mouse (Mus Musculus) liver tissue fixed using 10% NBF and Bouin solutions. This study used a descriptive method. The population in this study were mice. The research sample was mouse liver organs. The research stages began with mouse surgery, fixation with 10% NBF and Bouin, dehydration, clearing, embedding, blocking, cutting, and staining. The results of the histological features of mouse livers fixed using 10% NBF with a time variation of 4 hours obtained good quality preparations of 33.33% and poor quality of 66.67%. In 10% NBF with a time variation of 16 hours, good quality was obtained of 83.33% and poor quality of 16.67%. In liver tissue preparations of mice (Mus musculus) fixed using Bouin's 4-hour variation, 83.33% of good quality preparations were obtained and 16.67% of poor quality preparations. In Bouin's 16-hour variation, 33.33% of good quality preparations were obtained, 50.00% of poor quality preparations and 16.67% of poor quality preparations were obtained. Suggestions for further research on the microscopic appearance of tissue fixed using Bouin's solution and other solutions other than 10% NBF with samples other than mouse liver organs (Mus Musculus).
Perbandingan Hasil Pemeriksaan Demam Tifoid Menggunakan Uji Widal pada Pasien Anak dan Dewasa di Puskesmas Polowijen Liliosa Londong; Yeni Avidhatul Husna; Erni Yohani Mahtuti
Jurnal Ilmu Kedokteran dan Kesehatan Indonesia Vol. 6 No. 1 (2026): Maret : Jurnal Ilmu Kedokteran dan Kesehatan Indonesia
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jikki.v6i1.8930

Abstract

Typhoid fever is an infectious disease caused by Salmonella typhi bacteria and generally affects the digestive tract. This bacterium is commonly found in areas with poor sanitation, and diagnosis requires a combination of clinical and laboratory examinations. Typhoid fever is caused by Salmonella typhi, primarily attacking the digestive system and typically presenting with a fever lasting more than one week. This study applied a quantitative descriptive approach with a total sampling technique based on all medical record data of typhoid fever patients treated at Polowijen Public Health Center in 2024, totaling 150 respondents. The results showed that most patients were children and adolescents, with the highest prevalence in the 3–19 age group, consisting of 45 children (30%) and 53 adolescents–adults (35.33%). The disease tended to affect females more than males, partly due to women's frequent involvement in preparing and handling food and beverages. Patients commonly presented with positive Widal titers and clinical symptoms such as fever, headache, nausea, and vomiting. Data analysis utilized SPSS and cross-tabulation covering sex, age, duration of fever, and Widal titers. The findings indicated an association between age, clinical symptoms, and laboratory results with typhoid fever incidence. There was a correlation between high Widal titers and prolonged fever duration. Patients with titers ≥1/320 generally experienced fever lasting more than 6 days, particularly among adolescents–adults, whereas children tended to have lower titers (1/80–1/160) and a shorter duration of fever, although some cases with higher titers lasted up to 8 days.
Co-Authors Akbar Dwi Kurniawan Amarani, Anggi Dwi Anggi Nur Wijayanti Anggraini, Fransiska Arsya Lingga Datu Aulidia, Rochilah Baco, Maria Velgiyanti Basyarrudin, Muhammad Chantika Putri Indah Sari Chotimah Chusnul Cindy Tanggu Humba Dewita Nggua Evi Dwi Prastiwi Fahru Rizal Isyafa Faisal Faisal Faisal Faisal Faisal Faisal Faisal Faisal Faisal Faisal Faisal Farahdita Devi Masyitoh Febriyanti Wora Kapero Firnanda Magfirah Fitria Wahyu Rahmadhani Galih Zatmiko Alan Gresela Rambu Kaleka Mali, Aquina Handhini Gema Arika Putri Hidhayah, Nurul Husnah, Yeni Avidhatul Ibaadillah, Afrihal Afiif Ignasius Kalos Indah Anggeriani Kapero, Febriyanti Wora Karismadani, Id'ha Lutfia Kristin Natalia Makatita Makatita Larasati, Marsella Liliosa Londong Lilla Maria Lud Waluyo Maria Diyah Maria Diyah Maria Srisuharny Maria Srisuharny Moh. Mirza Nuryady Muhammad Basyaruddin Muhammad Basyaruddin, Muhammad Muhammad Basyarudin Muhammad Masyhur Muhammad Masyhur Muhammad Masyhur Nadila Karno Ndapamuri, Alfenia Nggua, Dewita Nisa Ailmi Lidya Artha Revica Novi Yuniar Novitasari , Lilis NURUL AZIZAH Pehan, Laurensia Rivoniarti Ese Prasetya, Arita Ajeng Previta Zeisar Rahmawati Previta Zeizar Previta Zeizar Rahmawati Previta Zeizar Rahmawati Previta Zeizar Rahmawati Rachmawati, Idelia Diny Rahmawati, Previta Zeisar Rahmawati, Previta Zeizar Risna’im, Annisa Rohmania Rodolof, Claudya Putri Samsun hadi Sanjaya, Zahra Weny Saragih, Lenni Sari, Chantika Putri Indah Saroh, Putri Rohmah Muya Siska Nanda Widhaningrum Siska Nanda Widhaningrum Siti Aisyah Siti Aisyah Sofiah, Yayu Susilawati, Nasiro Syamsyinova BS, Andini Aulia Taquillah, Naura Silmy Widiatmoko, Muhamad Wulandari, Dewi Permani Ayu Wulandari, Lenni Ratna Tri Yeni Avidhatul Husna Yuniarreza Satriani