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Peningkatan Senyawa Fenolik Bekatul dengan SSF (Solid State Fermentation) sebagai Pencegah Kanker Yeni Kurniati; Slamet Budijanto; Lilis Nuraida; Fitriya Nur Annisa Dewi
Iptek Tanaman Pangan Vol 12, No 2 (2017): Desember 2017
Publisher : Puslitbang Tanaman Pangan

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Abstract

Rice bran is a by-product of rice-milling process. The rice bran is a coarse powder substance that is rich in bioactive components, including ferulic acid, ?-oryzanol, ? -sitosterol, tokotrienol/tocopherol, trisin, r-coumaric, sinapic, syiringic and phytic acid. The phenolic compounds of rice bran have potential of preventing cancer. The compounds could be increased by SSF (Solid State Fermentation). Enzymes produced by microbes during the process of fermentation are amylase, xylanase, protease, lacase, esterase, and ? -glucosidase. Enzymes which help increase the phenolic compounds in the rice bran include: lacase, esterase and ? - glucosidase. Factors affecting the amount of increases of phenolic compounds in SSF are the types of microbes being used and duration of fermentation.
Karakterisasi Isolat Bakteri Asam Laktat dari Mandai yang Berpotensi Sebagai Probiotik Aswita Emmawati; Betty Sri Laksmi; Lilis Nuraida; Dahrul Syah
agriTECH Vol 35, No 2 (2015)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (521.517 KB) | DOI: 10.22146/agritech.9400

Abstract

Mandai is a fermented product made of cempedak (Artocarpus champeden) dami. The research aimed to isolate and characterize lactic acid bacteria isolated from  and to evaluate their probiotic potency.  samples were collected from several home industries in East Kalimantan area lactic acid bacteria (LAB) isolates were obtained from  on day 4, 8, and 12 fermentation and assessed for their probiotic properties. All isolates showed good tolerances towards low pH (pH 2.0) with the decrease of viable counts of less than 2 log cfu/ml. The LAB isolates could grow in the present of 0.5% bile salt although the viable counts decreased as compared to those in medium without bile salt. Reduction of viable counts less than 1 log cfu/ml was observed in 21 isolates. Nineteen isolates could tolerate pH 2.0 and 0.5% bile salt better than others with the total decrease in viable counts less than 1 log cfu/ml. Most of isolates (11 out of 19 isolates) which tolerate low pH were obtained from 8 days  fermentation. Isolate MC812 and MC809 had good antimicrobial properties against Listeria monocytogenes ATCC 13932, Enterococcus faecalis ATCC 19433, Bacillus cereus ATCC 10876, Escherichia coli ATCC 25922 and Salmonella typhimurium ATCC 14028. Ten isolates had good antimicrobial properties againts at least 3 pathogens tested. Their resistance toward antibiotics varied between isolates. All isolates was identified as Lectobacillus plantarum with API 50 CHL and confirmed with real-time-PCR. The overall results indicated that certain LAB isolates obtained from mandai show promising probiotic properties. ABSTRAKMandai merupakan pangan fermentasi yang terbuat dari dami atau bagian dalam kulit cempedak. Penelitian tentang mandai, khususnya bakteri yang terlibat dalam fermentasi mandai, masih belum banyak dilakukan. Tujuan penelitian ini adalah untuk melakukan isolasi dan karakterisasi bakteri asam laktat yang diisolasi dari  serta mengevaluasi potensinya sebagai probiotik. Sampel  diperoleh dari beberapa industri rumah tangga di Kalimantan Timur, yang dibuat dengan kadar garam 5, 10 dan 15%. Delapan puluh lima isolat bakteri asam laktat diperoleh dari pada hari ke-4, 8 dan 12 fermentasi dan dikaji sifat-sifat probiotiknya. Semua isolat menunjukkan toleransi yang baik terhadap pH rendah (pH 2,0) dengan penurunan jumlah sel hidup kurang dari 2 log cfu/ml. Isolat bakteri asam laktat dapat tumbuh dengan adanya 0,5% garam empedu walaupun jumlah sel hidupnya menurun dibandingkan dengan jumlah sel hidup pada medium tanpa garam empedu. Penurunan jumlah isolat viabel kurang dari 1 log cfu/ml teramati pada 21 isolat. Sembilan belas isolat dapat mentoleransi pH 2,0 dan garam empedu 0,5% lebih baik daripada yang lain dengan total penurunan jumlah sel hidup kurang dari 1 log cfu/ml. Sebagian besar isolat (11 dari 19) yang mentoleransi pH rendah diperoleh dari fermentasi  hari ke-8. Isolat MC812 dan MC809 mempunyai sifat antimikroba yang baik terhadap semua patogen uji (Listeria monocytogenes ATCC 13932, Enterococcus faecalis ATCC 19433, Bacillus cereus ATCC 10876, Escherichia coli ATCC 25922 dan Salmonella typhimurium ATCC 14028). Sembilan isolat lain mempunyai sifat antimikroba yang baik terhadap 3 atau lebih patogen uji. Resistensi terhadap antibiotik bervariasi diantara isolat. Kesepuluh isolat diidentifikasi sebagai Lactobacillus plantarum dengan API 50 CHL dan dikonfirmasi dengan real-time-PCR. Keseluruhan hasil mengindikasikan bahwa kesepuluh bakteri asam laktat yang diisolasi dari mandai berpotensi sebagai probiotik.
Aktivitas Antimikroba Minyak Esensial Jahe Merah (Zingiber officinale var. Rubrum) dan Lengkuas Merah (Alpinia purpurata K. Schum) terhadap Bakteri Patogen dan Perusak Pangan Tita Rialita; Winiati Pudji Rahayu; Lilis Nuraida; Budi Nurtama
agriTECH Vol 35, No 1 (2015)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.206 KB) | DOI: 10.22146/agritech.9418

Abstract

The aims of this study was to determine the characteristics, composition and antimicrobial activity of essential oils of local Indonesian red ginger and red galangal against four pathogenic and food spoilage bacteria, which were B. cereus ATCC 10876, E. coli ATCC 25922, S. typhimuriumATCC 14028, and P. aeruginosa ATCC 27853. Analysis of physicochemical characteristics was carried outin accordance with ISO7355:1985. The chemical compositionwas analyzed using aGC-MS. The antimicrobial activity was determined by disc diffusion method and broth microdillution method was used for determine MIC and MBC values. Red ginger essential oil characteristic was brownish yellow, specific gravity 0.883, refractive index 1.480, optical rotation -8.45o, clear soluble (1:1) in 90 % alcohol, 2.06 acid number and 42.45 ester number. Redgalangal essential oil had a characteristic bright yellow color, specific gravity 0.895, refractive index 1.496, optical rotation -9.15o, clear soluble (1:1) in 90 % alcohol, 1.95 acid number and 140.15 ester number. The major component of red ginger essential oils were trimethyl-heptadien-ol, ar-curcumene, camphene, carbaldehyde, sesquiphellandrene, and nerol; while the major component of red galangal essential oil were 1.8-cineole, chavicol, 9-desoxo-9-xi-hydroxy-3-pentaacetate-3,5,7,8,9,12-Ingol, -caryophyllene and -selinene. The essential oil of red ginger and red galangal hadmoderate antibacterial activity against pathogenic and food spoilage bacteria with the average inhibition zone 7.17-10.33 and 7.25-11.17mm. Red ginger essential oils could inhibit the growth of tested bacteria with MIC values of 2.65-3.97 mg/mL and MBC value of 3.10-5.29 mg/mL, while the red galangal essential oil could inhibit the growth of tested bacteria with MIC values of 1.79-4.03 mg mL and MBC values of 1.79-4.92 mg/mL. Based on the MIC and MBC values, all tested bacteriasensitivity to essential oils of red ginger and galangal red decline in a row B.cereus > E. coli > S. typhimurium> P. aeruginosa. Sensitivity of Gram positive and Gram negative bacteria to both essential oils demonstrate the potential of the oils to be used as a natural preservative in the food industry. ABSTRAKPenelitian ini bertujuan untuk mengetahui karakteristik, komposisi dan aktivitas antimikroba minyak esensial jahe merah dan lengkuas merah lokal Indonesia terhadap empat spesies bakteri patogen dan perusak pangan, yaitu B.cereus ATCC 10876, E.coli ATCC 25922, S. typhimurium ATCC 14028, dan P. aeruginosa ATCC 27853. Analisis karakteristik fisika-kimia dilakukan sesuai standar ISO 7355:1985. Komposisi kimia dianalisis menggunakan alat GC-MS. Pengujian aktivitas antimikroba dilakukan dengan metode difusi cakram untuk menentukan zona hambat, sertabroth microdillution untuk menentukan nilai Minimum Inhibitory Concentration (MIC) dan Minimum Bactericidal Concentration (MBC). Karakteristik minyak esensial jahe merah yang dihasilkan yaitu kuning kecoklatan, berat jenis 0,883, indeks bias 1,480, putaran optik -8.45, larut jernih (1:1) dalam alkohol 90%, bilangan asam 2,06, dan bilangan ester 42,45. Minyak esensial lengkuas merah memiliki karakteristik warna kuning terang, berat jenis 0,895, indeks bias 1,496, putaran optik -9.15, larut jernih (1:1) dalam alkohol 90%, bilangan asam 1,95 dan bilangan ester 140,15. Komponen mayor minyak esensial jahe merah terdiri dari trimethyl-heptadien-ol, ar-curcumene, camphene, carbaldehyde, -sesquiphellandrene, dan nerol; sedangkan komponen mayor minyak esensial lengkuas merah terdiri dari 1.8-cineole, chavicol,9-desoxo9-xi-hydroxy-3,5,7,8,9,12-pentaacetat-ingol, -caryophyllenedan -selinene. Minyak esensial jahe merah dan lengkuas merah memiliki aktivitas antibakteri yang bersifat moderat terhadap bakteri patogen dan perusak pangan, dengan kisaran zona hambat rata-rata 7,17-10,33 mm dan 7,25-11,17 mm. Minyak esensial jahe merah dapat menghambat pertumbuhan bakteri uji pada nilai MIC 2,65-3,97 mg/mL dan nilai MBC 3,10-5,29 mg/mL, sedangkan minyak esensial lengkuas merah dapat menghambat bakteri uji dengan nilai MIC 1,79-4,03 mg/mL dan nilai MBC 1,79-4,92 mg/mL. Berdasarkan nilai MIC dan MBC, sensitivitas bakteri uji terhadap minyak esensial jahe merah dan lengkuas merah menurun berturut-turut dari B. cereus > E. coli > S. typhimurium > P. aeruginosa. Sensitivitas bakteri Gram positif dan Gram negatif terhadap kedua minyak esensial ini menunjukkan potensi minyak esensial jahe merah dan lengkuas merah untuk digunakan sebagai pengawet alami di industri pangan.
Kajian Isolat Bakteri Asam Laktat dalam Menurunkan Kolesterol Secara In Vitro dengan Keberadaan Oligosakarida Yati Maryati; Lilis Nuraida; Ratih Dewanti Hariyadi
agriTECH Vol 36, No 2 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (590.675 KB) | DOI: 10.22146/agritech.12865

Abstract

This work evaluated the abilities of five isolates of lactic acid bacteria (LAB) from different sources, i.e Lactobacillus fermentum S21209 and Lactobacillus plantarum 1-S27202 from tempe, Lactobacillus rhamnosus R23 and Pediococcus pentosaceus 1-A38 from human breast milk and a commercially available human isolates Lactobacillus acidophilus FNCC0051 in lowering cholesterol by in vitro and metabolizing the prebiotic oligosaccharide compounds. The effects of oligosaccharide compounds on the performance of the LAB isolates in lowering cholesterol were also evaluated. The tests were done in MRS based medium in vitro with or without oligosaccharides i.e. galactooligosacharrides (GOS), fructooligosaccharides (FOS), inulin, hydrolyzed inulin or combination of oligosaccharides as prebiotics. The results revealed that all isolates were able to reduce cholesterol in the medium, and the highest cholesterol reduction was observed for L. acidophilus FNCC0051 and L. rhamnosus R23. There are two different mechanism in the loweringof cholesterol; cholesterol assimilation and cholesterol binding on the cell surface. For the case of P. pentosaceus 1-A38, it involves the assimilation, while the other four isolates may involve cholesterol binding on the cell surface. In addition, the tested LAB’s has different ability to use prebiotics, as shown by the reduction of total sugar in the medium. Oligosaccharides metabolism by L. acidophilus FNCC0051 and L. rhamnosus R23 resulted in several organic acid and SCFA with lactic acid produced as the largest proportion followed by acetic acid. Furthermore, the proportion of propionic and butyric acids were influenced by the type of isolates and carbon source. L. acidophilus FNCC 0051 was able to reduce cholesterol in the MRS based medium with oligosaccharides and their combination as carbon source and cholesterol reducing ability seems to involve both assimilation and cholesterol binding on the cell surface.ABSTRAKPenelitian ini mengevaluasi lima isolat bakteri asam laktat (BAL) dari sumber yang berbeda, yaitu Lactobacillus fermentum S21209 dan Lactobacillus plantarum 1-S27202 dari tempe, Lactobacillus rhamnosus R23 dan Pediococcus pentosaceus 1-A38 dari ASI dan isolat komersial Lactobacillus acidophilus FNCC 0051 dari percernaan manusia dalam kemampuannya menurunkan kolesterol secara in vitro dan kemampuannya memetabolisme senyawa oligosakarida prebiotik. Pengaruh senyawa oligosakarida terhadap kemampuan isolat BAL terpilih untuk menurunkan kolesterol juga dievaluasi. Pengujian dilakukan pada media berbasis MRS dengan atau tanpa oligosakarida terdiri dari galaktooligosakarida (GOS), fruktooligosakarida (FOS), inulin, hidrolisat inulin atau kombinasi oligosakarida sebagai prebiotik. Hasil penelitian menunjukkan bahwa semua isolat mampu menurunkan kolesterol, dan penurunan kolesterol tertinggi ditunjukkan oleh isolat L. acidophilus FNCC0051 dan L. rhamnosus R23. Penurunan kolesterol diduga terjadi melalui dua cara yang berbeda. Mekanisme penurunan kolesterol oleh isolat P. pentosaceus 1-A38 melibatkan asimilasi kolesterol, sedangkan pada keempat isolat lainnya kemungkinan melibatkan pengikatan kolesterol pada permukaan sel. Selain itu, isolat BAL juga memiliki kemampuan yang berbeda dalam memanfaatkan oligosakarida prebiotik, terlihat pada penurunan total gula dalam medium. Metabolisme senyawa oligosakarida oleh L. acidophilus FNCC0051 dan L. rhamnosus R23 menghasilkan beberapa asam organik termasuk SCFA dengan proporsi terbesar asam laktat diikuti oleh asam asetat. Selain itu, proporsi asam propionat dan butirat dipengaruhi oleh jenis isolat dan sumber karbon. L. acidophilus FNCC 0051 mampu menurunkan kolesterol dalam media berbasis MRS dengan keberadaan oligosakarida baik tunggal maupun kombinasi sebagai sumber karbon dan melibatkan mekanisme baik asimilasi dan pengikatan kolesterol pada permukaan sel.
Sensory Profiles and Lactic Acid Bacteria Density of Tape Ketan and Tape Singkong in Bogor Uswatun Hasanah; Haqqifizta Ratihwulan; Lilis Nuraida
agriTECH Vol 38, No 3 (2018)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (800.382 KB) | DOI: 10.22146/agritech.30935

Abstract

Tape ketan (fermented glutinous rice) and tape singkong (fermented cassava) are traditional Indonesian foods produced by fermenting carbohydrate sources using ragi as starter culture. Those products known to contain high number of lactic acid bacteria (LAB). The purpose of the study was to evaluate the sensory characteristics of tape ketan and tape singkong from different household industry in Bogor, to identify the sensory profile, and to evaluate the correlation between LAB density and sensory attribute. The research was started by survey to household tape industries, followed by sensory analysis of rating hedonic and Quantitative Descriptive Analysis (QDA), and LAB analysis. Data analysis of rating hedonic was done by ANOVA and post-hoc Duncan test, while data analysis of QDA was done by Principle Component Analysis (PCA). Samples of tape ketan were taken from two different home industries (BK and LK), while samples of tape singkong were taken from four different home industries (CT, NG, SH, TS). Hedonic rating test showed that the most preferred sample overall was tape ketan BK and tape singkong CT. QDA results showed that tape ketan BK was characterized by its sweetness, rice-like aroma, rice-like taste, and texture, while tape ketan LK was characterized by alcoholic aroma, alcoholic taste, sourness, and color. Tape singkong NG were characterized by sour aroma, alcoholic aroma, alcoholic taste, and color. Specific characteristic of tape singkong CT was bitterness and bitter aftertaste, SH was sweetness, and TS was texture. LAB of tape ketan BK (8,5 log cfu/g) was higher than LK (7,9 log cfu/g). Tape singkong with highest density of LAB was TS (8,1 log cfu/g), followed by CT (7,2 log cfu/g), NG (7,0 log cfu/g), and SH (6,5 log cfu/g). There was no correlation between the density of LAB and descriptive score of sourness, alcoholic taste, and sweetness.
Kesiapan Usaha Mikro Kecil Menengah Pangan dalam Penerapan ISO 9001:2008 (Studi Kasus di Palu, Sulawesi Tengah) Tjahja Muhandri; Dian Herawati; Faleh Setia Budi; Lilis Nuraida; Sutrisno Koswara; Afifah Zahra Agista; Yuli Sukmawati
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 2 No. 2 (2016): Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.2.2.61-66

Abstract

Small and Medium Industries of the Food (Food SME’s) needs to have SNI products to be competitive in the free market era. SNI certificate product can be provided by the certification body, on the condition that the industry has to implement ISO 9001. This activity aims to identify the readiness of small industries (food) to meet the clauses of ISO 9001 and assist them in improving the necessary documents. Activities carried out for 2 months with 24 samples of small industries (food) in Palu (Central Sulawesi) were selected purposively. The results showed that all the small industries yet to implement all aspects of GMP. Small industry best only meet 21% of the clauses of ISO 9001. Compliance with the clauses of ISO 9001 increased by between 4586% after mentoring.
Identifikasi Sumber Glutamat Bebas pada Menu Hidangan Indonesia Nuri Andarwulan; Lilis Nuraida; Purwiyatno Hariyadi; Siti Madanijah; Zulaikhah Zulaikhah; Desty Gitapratiwi
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 2 No. 1 (2015): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

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Abstract

The study aimed to evaluate free glutamate content occurs naturally in prepared foods or dish menus. Fifteen selected Indonesian dish menus were categorized to three major groups, i.e. dish menu with monosodium glutamate (MSG) addition, dish menu with seasoning and/or condiment containing glutamate addition, and dish menu with MSG, seasoning and/or condiment containing glutamate addition. Each dish menu was prepared at the kitchen laboratory by standardizing the ingredients, composition and cooking process, followed by analysis of free glutamate content in dish menu, its raw material mix, and basic seasoning using HPLC method. The study results showed that raw materials such as chicken, fermented-tofu solid waste (oncom), meatball, cabbage, fried onion, tomato, and fried potato contributed to the free glutamate content in dish menus. Addition of MSG, seasoning and/or condiments de nitely contributed to free glutamate content in the dish menus, ranged 9.8-142.9% for MSG added and 7.9- 100% for seasoning and/or condiments added. The dish menus with MSG addition which contributed to higher free glutamate content were fried tempe, rice cake with vegetable soup in coconut milk, and spiced chicken soup. Meanwhile, the dish menus with seasoning/ condiment addition contributed to higher free glutamate content were stir-fry water crest, mixed vegetable in chicken soup, chicken rice porridge. Cooking practices could increase or reduce free glutamate content in dish menus, ranged from -86.2 to 43.3%. 
Pengaruh Perbedaan Jenis Kedelai terhadap Kualitas Mutu Tahu Nuri Andarwulan; Lilis Nuraida; Dede R. Adawiyah; Ria Noviar Triana; Denny Agustin; Desty Gitapratiwi
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 5 No. 2 (2018)
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

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Abstract

Tofu is a processed product which is produced by coagulation process of soybean protein extract. The objectives of this study were to evaluate the relation of soybean variety to physicochemical characteristics during processing and the charactiristics of tofu. The soybean (IP new, IP 1 year old, US, Argentina, Indonesia) had moisture content (8.67-9.95%), ash content (5.15-5.36%), protein content (30.33-36.49%),  fat content (15.91-25.11%) and carbohydrate (26.98-35.00%). The highest yield of tofu was found in soybean IP New that was 195.60%, with tofu water content (80.18-83.17%), protein content (8.83-10.69%), and fat content (3.94-6.31%). The highest total solid was found in tofu from Argentina soybean that was 19.82%, while the density of tofu relatively similar (1%) to other tofu. The highest protein recovery and total solid recovery were found in tofu from Argentina soybean (62.05% and 39.62%). Argentina soybean was the best soybean which can be used to produce tofu. That soybean had the best protein recovery and based on texture quality analysis the soybean had similar characteristics with IP New soybean which had the best hardness, elasticity, and cohesiveness.
Penerapan Audit Internal Terpadu dalam Sistem Manajemen Terpadu Berdasarkan PAS 99:2012 Di Industri Perisa Pika Mustika; Lilis Nuraida; Harsi Dewantari Kusumaningrum
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 3 No. 2 (2016): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

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Abstract

Individually implementation of management system standard of quality (ISO 9001), food safety (FSSC 22000), and halal assurance (HAS 23000) require a lot of resources in a food company. This study aimed to develop perangkat of internal audit (IA) by integrating the three standards into an Integrated Management System (IMS) based on the management system requirements framework, PAS 99:2012. The study was started by integrating all the major requirements of standards follows the Deming's PDCA cycle approach consisting of 7 clauses. The perangkat of IA was developed based on the IMS and implemented in PT IBP. Evaluation the effectiveness of the perangkat of IMS IA is done by analyzing the gap of IA results with external audit results. For integration of the standards, the most standard used as a case of IMS was the standard of FSSC 22000. The result of IA using the developed perangkat of IA was in agreement with the result of external audit. This suggest that the integration of the audit system has adequately covered all requirements based on the 3 standards. In addition, there is an opportunity to improve the ability of internal auditors in assessing the conformity of the implementation of planning.
Analisis Pemenuhan Persyaratan Food Savety System Certification 22000 di Industri Kemasan Pangan Jamal Zamrudi; Harsi Dewantari Kusumaningrum; Lilis Nuraida
Jurnal Mutu Pangan : Indonesian Journal of Food Quality Vol. 1 No. 2 (2014): Jurnal Mutu Pangan
Publisher : Department of Food Science and Technology (ITP), Faculty of Agricultural Technology, Bogor Agricultural University (IPB) in collaboration with the Indonesian Food and Beverage Association (GAPMMI), the National Agency of Drug and Food Control, and th

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Abstract

Food Safety System Certification 22000 (FSSC 22000) as food safety management system recently becomes requisition required by customers, consisting of ISO 22000, PAS 223:2011, and additional requirements. The purpose of this study was to develop recommendations and establish a simple strategy model in compliance with FSSC 22000 requirements. The methods used were identifying food safety regulations related to FSSC 22000, assessing the actual condition of PT XYZ as a case study, developing recommendations on the found gaps, and developing a simple strategy model. PT XYZ has ful lled 63% of the requirements, with in detail compliance of ISO 22000, PAS 223: 2011, and their additional requirements were 55%, 70%, and 20% respectively. Food safety management system has not been well socialized that caused lacking of personnel awareness, infrastructure and documentation implemention, and food safety requirement for supplier selection. The strategy to meet the food safety system requirement incudes action, plan, training, infrastructure improvement, documentation review, food safety training for suppliers, internal audit, and external audit. It was concluded that the strategy model developed has been effective in helping the company to meet the FSSC 22000 requirements. 
Co-Authors . EFRIWATI . Hana Afifah Zahra Agista Alam, Syah Khalif Amor Tresna Karyawati, Amor Tresna Anja Meryandini Antonius Suwanto Antung Sima Firlieyanti Antung Sima Firlieyanti Apriliana W. Hartanti Ardiansyah . Arimah Arimah Bambang Pontjo Priosoeryanto Betty Jenie Sri Laksmi Betty Sri Laksmi Betty Sri Laksmi Jenie Betty Sri Laksmi Jenie Budi Nurtama Cece Sumantri Cynthia Rizka Riani Dahrul Syah Dede R. Adawiyah Dede Robiatul Adawiyah Denny Agustin Desty Gitapratiwi Desty Gitapratiwi Dewi, Fitriya Nur Annisa Dewi, Retno Anggrina Khalistha Dian Herawati Dias Erfan Dodik Briawan Donna Fujie Rahaditha Utami Dreitsohn Franklyn Purba Eko Hari Purnomo Emmawati, Aswita Endang Prangdimurti Epi Taufik Fadlillah, Hendry Noer Faleh Setia Budi Farida, Eko Fenny Larasati Firat Meiyasa Gayuh Rahayu Hana - - Hana . Hana . Hanifah Nuryani Lioe Haqqifizta Ratihwulan Harsi D. Kusumaningrum Hendry Noer Fadlillah Iffa Illiyya Fatma Irma Isnafia Arief Jamal Zamrudi Madaniyah, Siti Maggy T. Suhartono Maggy Thenawidjaja Suhartono Mahara, Fenny Amilia Mochammad Sriduresta Soenarno NASRIANTI SYAM, NASRIANTI Ni Nyoman Puspawati Nur Rita Mardiana Nurbety Tarigan Nurdin, Qamariyah Nuri Andarwulan Nurwulan Purnasari Pika Mustika Purwiyatno Hariyadi Puspo Edi Giriwono Qamariyah Nurdin Raini Panjaitan Ratih Dewanti -Hariyadi Ria Noviar Triana Rina Ningtyas Safrida Safrida Safrida Safrida Siti - Winarti Siti Madanijah Siti Madaniyah Siti Nurjanah Slamet Budijanto Sri Budiarti Poerwanto Sri Rini Dwiari Suci Apsari Pebrianti Sutrisno Koswara TATI NURHAYATI Tio, Yohana Tania Tita Rialita Tjahja Muhandri Ummul Khayrah Uswatun Hasanah Utami, Donna Fujie Rahaditha Widya Aprilita Minamilail Winiati P Rahayu Winiati P Rahayu Winiati P. Rahayu Winiati P. Rahayu Winiati Pudji Rahayu Yati Maryati Yati Maryati Yeni Kurniati Yeni Kurniati Yeni Restiani Yuli Sukmawati YULIN LESTARI Zamrudi, Jamal Zulaikhah Zulaikhah Zulaikhah Zulaikhah Zulaikhah Zulaikhah, Zulaikhah