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Jurnal Analis Medika Biosains (JAMBS)
Published by Poltekkes Kemenkes Mataram
ISSN : 23564075     EISSN : 26562456     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions
JAMBS (Jurnal Analis Medika Bio Sains) is a journal that provides a forum for publishing articles related to food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology. Scientific articles dealing with the following topics in food analysis, mikrobiology, hematolgy, clinical chemistry, parasitology, immunoserology, histology.
Arjuna Subject : -
Articles 313 Documents
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Perbedaan Waktu Penambahan Reagen AHG Berpengaruh Terhadap Hasil Pemeriksaan Uji Silang Serasi Metode Tabung Rassajati, Syaqina; Mentari, Diani; Pebrina, Relita; Prasetya, Hieronymus Rayi
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 1 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i1.267

Abstract

Transfusi darah adalah proses pemberian darah dari seseorang (donor) kepada orang lain (resipien) yang bertujuan untuk mengganti darah hilang akibat perdarahan, mengatasi shock, memelihara serta mempertahankan kesehatan resipien. Analisis pretransfusi khususnya pada uji serologi sangat diperlukan untuk menjamin darah yang ditransfusikan aman dan tidak menyebabkan reaksi transfusi. Proses pencocokan darah donor dan dengan darah pasien/resipien dilakukan melalui uji. uji silang serasi (crossmatch). Uji silang serasi dapat dilakukan melalui 2 metode yaitu yaitu menggunakan tabung dan gel card. Uji silang serasi metode tabung terdiri dari 3 fase yaitu fase 1, fase 2 dan fase 3. Pada Fase 3 merupakan tahapan yang paling berpengaruh, karena ada penambahan Anti Human Globulin (AHG). Reagen AHG berisi immunoglobulin yang berfungsi sebagai penghubung antibodi inkomplit (antibodi IgG yang menyelubungi eritrosit), sehingga akan terdeteksi melalui terbentuknya aglutinasi (gumpalan). Namun perlu diketahui bahwa ikatan antar antibodi tidak dapat bertahan lama. Ikatan antar antibodi (AHG dan antibodi inkomplit) dapat terlepas kembali (disosiasi), sehingga hasil pemeriksaan dapat menjadi false negatif. Tujuan penelitian ini adalah untuk mengetahui apakah waktu penambahan AHG mempengaruhi derajad aglutinasi pada saat uji silang serasi. Penelitian ini menggunakan metode uji silang serasi metode tabung dengan menggunakan golongan darah O rhesus positif sebagai sampel. Analisis data dilakukan melalui pengamatan derajat aglutinasi pada tiap fase uji silang serasi. Pada penelitian ini, adanya perbedaan waktu penambahan AHG mempengaruhi hasil pemeriksaan uji silang serasi. Kesimpulan dalam penelitian ini adalah efektifitas penambahan AHG pada uji silang serasi metode tabung yaitu pada menit ke 2-10 menit.
Effect of Soaking Time’s Variation of Red Onion (Allium Cepa L) Extracts on Formalin Content in Mackerel Tuna (Euthynnus Affinis) Lestyaninrum, Indah Denissa; Dhanti, Kurnia Ritma; Sulistiyowati, Retno; Kurniawan, Kurniawan
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.289

Abstract

Mackerel tuna (Euthynnus affinis) is one of the most popular foods in the community since it is affordable and has complete nutritional content. It is a frozen fish food product so the storage period of which is relatively short. In addition, it is possible to add formalin on which. Formalin is a substance that is prohibited from being used due to its dangerous effects. The content of saponin compounds found in red onions (Allium cepa L) can be used to reduce formalin levels. This study aimed to determine the effect of variations in the soaking time of red onion extract on the formalin level in mackerel tuna and the optimal soaking time for reducing formalin levels. This study used an experimental method with a posttest control group design. The treatment was given by soaking red onion extract at a concentration of 10% in mackerel tuna containing formalin with variations in soaking time of 1 hour, 2 hours, and 3 hours which were tested quantitatively using a UV-Vis spectrophotometer. Then, the data were analyzed using a one-way ANOVA statistical parametric test and a post hoc test using Duncan's test (DMRT). The decrease of the formalin levels by soaking the onion extract for 1 hour, 2 hours, and 3 hours was 10.61%, 41%, and 73%, respectively. The result showed that there was an effect of the soaking time of red onion extract on formalin levels (p-value 0.05), and the optimal time of soaking onion extract to reduce the formalin content of the samples was 3 hours.
Relationship Long Time Storage of Trunk Fish (Euthnymus Affinis) Smoke With The Presence Of Staphylococcus Aureus Bacteria At Bobotsari Traditional Market Romadhon, Zakiyatudin Anduko; Mulyanto, Arif; Mujahid, Ikhsan; Widodo, Oei Stefani Yuanita
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 2 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i2.325

Abstract

Fresh fish can experience spoilage due to the activity of enzymes and microorganisms contained in the body of the fish itself due to the oxidation process in body fat by air. The body of the fish contains water which is quite high, namely 60-80% and has a pH of 7.2 so that it can be a good medium for the growth of putrefactive bacteria. Preservation methods are carried out to reduce the presence of spoilage alternatif preservation can be done, among others, by salting, cooling, drying, and smoking. Excess fumigation contains phenol which can function as an anti-microbial so that microbes will die and not develop. The purpose of this study was to determine the relationship between long storage of smoked tuna (Euthynmus affinis) and the presence of Staphylococcus aureus bacteria. The sample in this study were 12 samples of smoked cob fish which were sold in the traditional markets of the ballpark and the sampling technique used was random sampling. This type of research was experimentally analyzed using the Kruskall Wallis test. The results of the study of 12 samples of tuna that contained Staphylococcus aureus bacteria concluded that there was a relationship between storage duration of smoked tuna (Euthynmus affinis) and the presence of Staphylococcus aureus bacteria.
Deteksi Metichilin Resistance Staphylococcus Aureus (MRSA) Pada Peralatan Medis Yang Digunakan Di Ruang Rawat Inap RSUD Provinsi NTB Pristianingrum, Santy; Zainiati, Baiq Lely; Muttaqin, Zainul; Puspita, Fergy Desy; Arman, Rolly
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 1 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i1.220

Abstract

Methicillin resistant Staphylococcus aureus (MRSA) adalah jenis bakteri yang timbul karena resistensi terhadap antibiotik, di pusat layanan kesehatan penyebaran bakteri-bakteri yang mengalami resistensi tersebut dapat melalui sentuhan pasien dengan tenaga medis atau peralatan medis yang digunakan di fasilitas layanan kesehatan. Penelitian ini bertujuan untuk mendeteksi keberadaan MRSA, resistensinya terhadap antibiotik serta mendeteksi keberadaan MRSA SSCmec tipe III. Sampel dari diafragma stetoskop dan manset tensimeter di ruang rawat inap RSUD Provinsi NTB, diperiksa secara mikrobiologi dan secara molekuler menggunakan teknik PCR (Polimerase Chain Reaction) menggunakan primer SSCmec Tipe III. Selama bulan Januari hingga Februari 2018 didapatkan sebanyak 20 sampel swab diafragma stetoskop dan 18 swab manset tensimeter. Pengambilan sampel dilakukan pada akhir Januari hingga pertengahan Februari 2018.  Hasil penelitian ditemukan sebanyak 2 isolat dari swab diafragma stetoskop (10%) dan 2 dari manset tensimeter (11.1%)  terdeteksi sebagai MRSA. Dari empat isolate tersebut semuanya masih sensitive terhadap Vancomycin, tiga isolate  resisten Clindamycin, Cefoxitin, dan Oxacilin, serta 1 isolat resisten Cefoxitin dan Oxacilin. Hasil pemeriksaan PCR pada 4 isolat MRSA menunjukkan tidak ditemukan MRSA SSCmec tipe III.
Efek protein rekombinan MPT64 Mycobacterium tuberculosis terhadap proliferasi sel T dan Sel B secara in vitro Fihirrudin, Fihirrudin; Inayati, Nurul; Nidhal, Lalu Unsun; Jannah, Raudatul
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 2 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i2.278

Abstract

Mycobacterium protein tuberkulosis (MPT) 64 merupakan protein imunogenik yang disandi oleh gen Rv1980c dan berada pada lokasi Regions of Diffrences (RD) 2. Protein MPT64 di prediksi sebagai antigen dari M. tuberculosis yang pertama kali berinteraksi dengan sistem imun tubuh dari host. Penelitian ini bertujuan untuk mengetahui proliferasi sel T dan sel B yang di induksi dengan protein MPT64 secara invitro. Penelitian ini merupakan penelitian ekperimental yaitu melakukan uji proliferasi terhadap sel T dan sel B yang di induksi dengan protein MPT64 pada media RPMI. Purifikasi protein MPT 64 dilakukan dengan kolom Protino™ Ni-NTA System. Uji proliferasi sel T dan sel B dilakukan dengan Metode MTT, dimana hasil optical dencity nya (OD) di baca dengan ELISA reader pada panjang gelombang 595 nm. Data nilai Optical dencity hasil uji MTT di analisis dengan uji statistik One Way Anova. Hasil menunjukkan bahwa Rata-rata OD proliferasi sel T yang di induksi dengan protein MPT64 10 μg/ml, 5 μg/ml, 2,5 μg/ml dan 1,25 μg/ml masing-masing adalah 0.276, 0.202, 0.184 dan 0.178. Rata-rata OD proliferasi sel B yang di induksi dengan protein MPT64 10 μg/ml, 5 μg/ml, 2,5 μg/ml dan 1,25 μg/ml masing-masing adalah 0.434, 0.380, 0.285 dan 0.251. Hasil uji statistik menunjukkan perbedaan yang signifikan pada setiap perlakuan dengan nilai probabilitas (p=0,011) <0,05. Protein MPT64 konsentrasi 10 μg/ml menunjukkan hasil yang paling optimal dalam menginduksi proliferasi sel T dan sel B secara invitro.
Relationship Between Albumin Levels and Leukocyte Counts in The Incident of Neonatal Sepsis Sulaiman, Shofan Ilham; Haryanto, Edy; Endarini, Lully Hanni
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 2 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i2.313

Abstract

Background: Sepsis can affect all age, but is most common in young infants and neonates. Young infants and neonates are more susceptible to infection because their immune systems are immature, unable to localize infections, and lack the IgM needed to protect the body against bacterial infections. When experiencing neonatal sepsis, there is often an increase in leukocytes in response to infection and inflammation, where inflammation can also cause a decrease in albumin in the blood. Objective: This study aims to analyze the relationship between albumin levels and leukocyte counts in the incidence of neonatal sepsis. Methods: The research method used analytic correlation with a cross sectional approach. A sample of 31 patients with neonatal sepsis who were treated at Waluyo Jati hospital, Probolinggo Regency in December 2022 - April 2023 were sampled using saturation sampling method. Results: The results of the study on neonatal sepsis based on laboratory examinations mostly occurred in conditions of leukocytosis (45.2%) and hypoalbuminemia (77.4%), while the results of the study based on Pearson correlation test analysis obtained a significant value (p) of 0.737 (α=0.05). Conclusion: there is no relationship between albumin levels and leukocyte counts in neonatal sepsis.  
Resistance Analysis Of Several Antibiotics in Samples of Clinical Isolates With Salmonellosis Wahyuni, Shohifatul; Pauzi, Iswari; Jiwintarum, Yunan; Fihiruddin, Fihiruddin
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.357

Abstract

Typhus is a systemic infection caused by Salmonella typhi bacteria, which is usually transmitted through contaminated food or drink, usually treated with antibiotics. Inappropriate use of antibiotics causes various problems, one of which causes resistance. This resistance problem has become a global problem, including in Indonesia where there is an increase in bacterial resistance from 2013 which is 40%, 2016 as much as 60% and in 2019 reached 60.4%, due to irrational use of antibiotics so that bacteria become resistant to drugs. This study aims to determine the description of resistance to several antibiotics in clinical isolates of patients with Salmonellosis This study is a pre-experimental study, with a cross sectional approach. The samples used were pure isolates of Salmonella typhi bacteria obtained from blood samples of Salmonellosis patients, then tested for resistance with 5 different types of antibiotics (Ciprofloxacin, Amoxicillin, Levofloxacin, Cefotaxime and Sulfamethoxazolle-trimethopim) with the disk method (Kearby Beaure). The number of experimental units is 25 units, with the number of replications is 5 replications. The results showed that the five types of antibiotics were sensitive to Salmonella typhi bacteria with the highest antibiotic sensitivity being Ciprofloxacin while the antibiotic that had the lowest sensitivity was Amoxicillin. From the results, it can be concluded that the resistance test of the five antibiotics (Ciprofloxacin, Amoxicillin, Levofloxacin, Cefotaxime and Sulfamethoxazolle-trimethopim) is sensitive. 
Analisis Mutu Pelayanan Terhadap Tingkat Kepuasan Pasien Di Laboratorium Rumah Sakit Umum Daerah Kota Mataram Santoso, Teguh; Fikri, Zainal; Jiwantoro, Yudha Anggit
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 1 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i1.223

Abstract

Rumah sakit merupakan salah satu bentuk sarana kesehatan, baik yang diselenggarakan oleh pemerintah maupun masyarakat yang berfungsi untuk melakukan upaya kesehatan dasar atau kesehatan rujukan dengan meningkatkan mutu pelayanan. Mutu pelayanan kesehatan merupakan faktor penting dalam keberhasilan suatu rumah sakit, dimana terdapat pelayanan laboratorium yang tidak dapat terpisahkan dengan rumah sakit. Pelayanan yang diberikan laboratorium sangat berpengaruh terhadap tingkat kepuasan pasien. Cara pengukuran tingkat kepuasan pasien menggunakan lima dimensi mutu pelayanan seperti, bukti fisik (tangible), kehandalan (reliabilty), ketanggapan (responsiveness), jaminan (assurance), dan empati (empathy). Mengetahui  mutu pelayanan terhadap tingkat kepuasan pasien di laboratorium RSUD Kota Mataram. Jenis penelitian ini adalah survei analitik dengan rancangan cross sectional. Populasi dalam penelitian ini adalah pasien laboratorium RSUD Kota Mataram, sampel penelitian sebanyak 98 orang. Tekknik pengambilan sampel purposive sampling dengan kriteria sampel. Pengumpulan data menggunakan kuesioner tentang pengaruh mutu pelayanan terhadap tingkat kepuasan pasien. Analisis data dengan uji chi square. Hasil penelitian karakteristik responden berdasarkan lima dimensi mutu pelayanan, didapatkan hasil variabel mutu pelayanan: tangible baik (67,4%), tidak baik (32,6%), reliability baik (83,7%), tidak baik (16,3%), responsiveness baik (94,0%), tidak baik (6,0%), assurance baik (51,1%), tidak baik (48,9%), dan empathy baik (97,0%), tidak baik (3,0%). Variabel kepuasan pasien responden yang mengatakan puas sebesar (74,5%). Hasil penelitian menunjukkan p value (0.000). Ada pengaruh mutu pelayanan terhadap tingkat kepuasan pasien di laboratorium RSUD Kota Mataram.
Perbedaan Jumlah Trombosit Pada Produk Trombosit Konsentrat (TC) Yang Berasal Dari Kantong Darah 350 Ml Dan 450 Ml di UTD PMI Kota Surabaya Tahun 2019 Amalia, Yustisia; Samsulhadi, Wiwid; Darmawati, Darmawati
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 1 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i1.243

Abstract

Salah satu produk darah yang di hasilkan oleh UTD adalah trombosit, Untuk menjamin kualitas dan kuantitas trombosit dapat dinilai dari jumlah trombosit, suhu simpan, goyangan, permeabilitas kantong darah, dan pH serta ada tidaknya kontaminasi bakteri. Trombosit didapat dari Whole Blood( WB ) yang ditampung ke dalam sistem kantong darah steril dengan kantong transfer yang terintegrasi, kandungan trombosit tersuspensi didalam plasma, pengawasan mutu komponen trombosit dapat dilakukan dengan pemeriksaan jumlah trombosit terhadap produk TC yang telah dihasilkan.Tujuan penelitian ini adalah untuk mengetahui perbedaan jumlah trombosit per unit pada produk TC antara volume kantong darah 350 ml dan 450 ml. Penelitian menggunakan metode analitik observasional dengan penedekatan cross-sectional. Data yang didapatkan dianalisa secara langsung, penelitian ini dilaksanakan pada bulan Februari  sampai October 2019  di Laboratorium Unit Transfusi Darah Palang Merah Indonesia Kota Surabaya. Sampel yang digunakan untuk penelitian ini merupakan hasil data pemeriksaan sampel produk TC yang berjumlah 44 sampel produk TC.Dari hasil penelitian membuktikan bahwa Jumlah trombosit dalam suatu produk TC yang dapat dikoleksi, dapat dipengaruhi oleh volume kantong darah whole blood sebagai bahan awal dari pembuatan suatu produk TC.
Analisis Jumlah dan Jenis Bakteri Coccus Gram Positif pada Sisa Sampel Darah di Spuit Arfi, Sofiyan Dwi; Kristinawati, Erna; Khusuma, Ari
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 2 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i2.270

Abstract

.Background: One of the problems in the field of health services that can increase the mortality rate is nosocomial infection. Waste containing pathogenic bacteria can serve as a medium for the spread of disease for health facility workers, sufferers and the public. One of the medical devices that have a high risk factor as an intermediary for infection is a syringe. Bacterial contamination of the syringe after use cannot be ignored. Therefore, further research is needed on waste in health facilities to determine infectious diseases caused by residual blood samples in the syringe. Research Objectives: To determine the presence of gram-positive coccus bacteria in the remaining blood sample in the syringe. Methods: This study used a descriptive observational method with a cross sectional approach to time. The sample size in this study was 14 samples with a sampling technique that is total sampling. Results: It was found that Staphylococcus aureus and Staphylococcus cohnii were found each of the 14 samples. Conclusion: There are gram-positive coccus bacteria, namely Staphylococcus cohnii and Staphylococcus aureus. 

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