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Contact Name
Fera Sartika
Contact Email
mohammadrizkifadhilpratama@gmail.com
Phone
+6287815093560
Journal Mail Official
lp2m@umpalangkaraya.ac.id
Editorial Address
Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya Building B 1st Floor RTA Milono St. Km.1,5 Palangka Raya 73111 INDONESIA
Location
Kota palangkaraya,
Kalimantan tengah
INDONESIA
Borneo Journal of Medical Laboratory Technology
ISSN : -     EISSN : 26226111     DOI : https://doi.org/10.33084/bjmlt.v2i2
Core Subject : Health, Science,
Borneo Journal of Medical Laboratory Technology is a Scientific Journal managed by Department of Medical Laboratory Technology Faculty of Health Science Universitas Muhammadiyah Palangkaraya and published twice a year (in October and April) by Institute for Researches and Community Services Universitas Muhammadiyah Palangkaraya, contains articles of research and critical-analysis studies in Blood-transfusion Science, Clinical Chemistry, Hematology, Histopathology, Immunology, Microbiology, Parasitology, Toxicology, Food and drink Analysis, Molecular Biology, and other Medical Laboratory aspects.
Articles 196 Documents
Pembuatan Sediaan Gel Arang Tempurung Kelapa Dan Uji Daya Hambat Terhadap Bakteri Klebsiella pneumoniae: Preparation of Coconut Shell Charcoal Gel and Inhibitory Test Against Klebsiella pneumoniae Bacteria Kamalah, Siti Rahmah; Kurniawan, Kurniawan; Mulyanto, Arif; Dhanti, Kurnia Ritma
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6082

Abstract

Klebsiella pneumoniae is a gram-negative rod-shaped bacterium that is a member of the Enterobacteriaceae group of bacteria and is pathogenic and can infect humans. Currently, many K. pneumoniae bacteria are resistant to antibiotics, giving rise to new problems in the health sector that must be addressed immediately by utilizing biodiversity in the form of medicinal plants such as coconut trees, which can produce coconut shells that can be made into charcoal. The results of the phytochemical analysis show that coconut shell charcoal contains many active compounds that have been used in the cosmetic and health industries. The purpose of this study was to obtain an optimal and effective coconut shell charcoal extract gel formula for inhibiting the growth of K. pneumoniae bacteria. This study included a true experimental post-only control group with different gel concentration groups, namely 3%, 6%, and 9%. The optimal formulation of coconut shell charcoal extract gel is a gel with a concentration of 9%, and the results of the one-way ANOVA statistical analysis show that the treatment of the inhibition test on K. pneumoniae bacteria from gels with different concentrations shows that there is a mean difference in the area of the inhibition zone with a significant value of 0.033 (p<0.05). The results of the LSD post-hoc test showed that several treatment groups had significant values (p <0.05). Based on the results of the study, it can be concluded that the optimal formulation of shell charcoal extract gel is a gel with a concentration of 9%, while the most effective gel for inhibiting the growth of K. pneumoniae bacteria is a gel with a concentration of 3%.
Optimasi Suhu Annealing Gen Blaz Dari Bakteri Methicillin-Resistant Staphylococcus Aureus (MRSA) Pada Peralatan Medis: Optimization Annealing Temperature Gene blaZ of Bacterial Methicillin-resistant Staphylococcus aureus (MRSA) in Medical Equipment Listiani, Lia; Dhanti, Kurnia Ritma; Kurniawan, Kurniawan; Widodo, Oei Stefani Yuanita
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6083

Abstract

Methicillin-resistant Staphylococcus aureus is a strain of S. aureus bacteria that is resistant to methicillin-type penicillins. MRSA bacteria can cause nosocomial infections, which can be spread through medical equipment. One of the genes that encodes the resistance trait of S. aureus is the blaZ gene. The detection of the blaZ gene requires optimizing the annealing temperature of the primer used to obtain the optimal PCR band. This study aims to optimize the annealing temperature for the amplification of the blaZ gene DNA from Methicillin-resistant Staphylococcus aureus (MRSA) bacteria on medical devices. The method used in this study used a kit for bacterial extraction and Polymerase Chain Reaction(PCR). The results of the blaZ gene DNA amplification in all samples showed clear bands at temperatures of 52.0°C, 54.2°C, 58.7°C, 61.8°C, and 64.0°C; no smears were formed, and the electrophoretic visualization results on the PCR product showed the results of bands that were according to the target, namely 173 bp. The most optimal annealing temperature for amplification of the blaZ gene in MRSA bacteria from hospital medical equipment is 61.8°C.
Hubungan Tingkat Pengetahuan Ibu Dan Usia Pemberian Mp-Asi Dengan Kejadian Diare Pada Balita Wilayah Kerja Puskesmas Tampa Kabupaten Barito Timur : Relationship Between Level Of Maternal Knowledge And Age Of Feeding With Diarrhea Incidence In Toddlers, Tampa Health Center, East Barito Alghifari, Muhammad; Lestarisa, Trilianty; Yuliani, Ni Nyoman Sri; Sasmithae, Lia; Balyas, Abi Bakring
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6084

Abstract

Diarrhea is defined as a digestive disorder that has the characteristics of defecation 3 times or more during one day which produces a watery stool consistency and can be followed by blood. diarrhea cases that occurred in the East Barito area in 2019 were around 3,428 people for all ages and 1,584 toddlers. maternal knowledge and age of complementary feeding is one of the factors that cause diarrhea. The purpose of the study was to determine the relationship between the level of maternal knowledge and the age of complementary feeding with the incidence of diarrhea in toddlers. This research method is a cross sectional analysis with purposive sampling technique totaling 100 samples. Data were obtained through questionnaires. The results showed a percentage distribution of good knowledge level of 75%. The percentage of the age of giving MP-ASI is exactly 76%. Maternal knowledge and the incidence of diarrhea have a significant relationship with a p value of 0.001. Age of complementary feeding and the incidence of diarrhea have a significant relationship with a p value of 0.001. It is hoped that this research can be an evaluation material in health services.
Hubungan Faktor Risiko Usia Dengan Angka Kejadian Kanker Payudara Dan Tumor Jinak Payudara Di RSUD Wangaya Kota Denpasar Tahun 2019-2022: The Correlation Between Age Risk Factors And Incidence Rates Breast Cancer And Benign Breast Tumors In Hospitals Wangaya City Of Denpasar In 2019-2022 Gatsu, Putu Denny Andreana; Cahyani, A. A. Eka; Candra D, I Dewa Gede; Novitasari, Novitasari
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6085

Abstract

Breast cancer is a condition in which there is a loss of control and normal mechanisms in cells, which causes abnormal, uncontrolled, and rapid growth in breast/mammae tissue. One of the risk factors for the occurrence of breast cancer is age, where a person's risk of being diagnosed with breast cancer will continue to increase as a person gets older and peaks at the age of 80 years. In this study to analyze the correlation between age risk factors on the incidence of breast cancer and benign breast tumors. Analytical observational study with a cross-sectional design. The population is all medical record data for the diagnosis of breast cancer and benign breast tumors at the Wangaya Regional General Hospital, Denpasar City in January 2019 – December 2022. Samples were taken using a categorical descriptive method and analyzed using Chi-square. The highest frequency of breast cancer patients was at the age of 46 - 55 years, amounting to 20 patients (64.5%). The highest frequency of patients with benign breast tumors was at the age of 26-35 years, amounting to 24 patients (30.76%). Chi-square test results show that the probability value (p) <0.05 (p = 0.000). There is a significant correlation between age risk factors and the incidence of breast cancer and benign breast tumors at the Wangaya Regional General Hospital, Denpasar City.
Literature Review : Gambaran Efektivitas Pemeriksaan Keracunan Merkuri Dengan Sampel Biologis: Literature Review : Description of the Effevtiveness of Mercury Poisoning Examination Using Biological Sample Sinaga, Hakim; Wahid, Rifky Saldi A.; Raudah, Siti
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6086

Abstract

Mercury is one of the heavy metals with high toxicity and is included in the class of neurotoxic substances that can cause various damage to tissue and nerve cells. The proper use of biomarkers in blood, urine and hair can determine the effectiveness of the mercury poisoning examination in the laboratory. Purpose: This study aimed to describe the effectiveness of mercury poisoning examination using biological samples. Method: This study used a literature review with online database sources from the search engine Science Direct, PubMed, Google Scholar. Result, The cause of mercury poisoning was through the use of mercury in various industrial fields, mining activities, consumption of fish, shellfish and water that had been contaminated with mercury. Examination of mercury poisoning with blood and urine biomarkers was the main choice for acute exposure due to the direct use of mercury, especially in the work environment. Hair biomarkers were the main choice for chronic exposure, mainly fish consumption. The length of working time and the frequency of fish consumption had a significant influence on the high levels of mercury in the body, where the longer and more mercury exposure, the more risk a person was to experience mercury poisoning. Mercury levels in the blood can be detected within 2-3 days, in urine one week after exposure, and in hair, levels are persistent in the long term. Conclusion: The effectiveness of mercury poisoning examination using biological samples depends on the type and duration of exposure, where short-term exposure to high concentrations uses blood and urine biomarkers and long-term exposure using hair biomarkers.
Jamur Kontaminan Pada Nasi Berdasarkan Cara Penyimpanan: Contaminant Fungus In Rice Based On Storage Method Agustina, Shella; Azahra, Sresta; Kusumawati, Nursalinda
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6087

Abstract

Rice is a staple food for the people of Indonesia as a source carbohydrate. Improper rice storage can lead to contamination microorganisms. One of these microorganisms is fungus. Contaminant mold is an example of a type of fungus that can grow on foodstuffs such as rice. This study aims to determine the description of fungal contaminants in rice with various kinds of storage, namely rice cooker on, rice cooker off, and serving lid with storage time of 8 hours, 16 hours, and respectively 24 hours. This type of research is a descriptive observational approach cross sectional. The sample uses 3 brands of rice taken from the market Segiri Kota Samarinda with purposive sampling technique. Rice is processed then stored in various places and storage times. After that identification of contaminants macroscopically and microscopically. Data analyzed using univariate analysis. The results of the research show that there is 3 species of contaminating fungi namely Aspergillus sp, Muchor sp, and Penicillium sp. Based on the sample storage with rice cooker on, rice cooker off, and serving cap respectively indicate the percentage growth of the species the same fungus, namely 33.3%.
Gambaran Kadar Hemoglobin Pada Petani Buah Jeruk Pengguna Pestisida Di Desa Karangwidoro Kecamatan Dau Kabupaten Malang: Description of Hemoglobin Levels in Citrus Fruit Farmers Using Pesticide in Karangwidoro Village, Dau District, Malang Regency Azizah, Dinar Silky; Faisa, Faisa; Fatmawati, Diana Noor
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6088

Abstract

Hemoglobin is a protein that contains iron and is found in erythrocytes which functions as a carrier of oxygen from the lungs throughout the body. Hemoglobin also carries carbon dioxide back to the lungs to be exhaled out of the body. When the level of hemoglobin in the body is low, anemia is indicated. Low hemoglobin levels can be caused by the use of pesticides which are toxic chemicals and are often used by farmers to eradicate pests in the agricultural sector. Exposure to pesticides in the body causes the formation of sulfhemoglobin and methemoglobin binding which is the cause of the low amount of hemoglobin in red blood cells resulting in anemia. The purpose of this study was to describe the level of hemoglobin in citrus fruit farmers using pesticides in Karangwidoro Village, Dau District, Malang Regency. This research is a descriptive research with purposive sampling technique. The population is 76 farmers and the sample consists of 22 male farmers. Hemoglobin levels were checked using the Easy Touch Hemoglobin tool. The normal value of hemoglobin level is 13.0 g/dL-17.0 g/dL. The results showed that Hemoglobin levels in citrus fruit farmers using pesticides ranged on average 14.9 g/dL, the lowest value was 11.5 g/dL, and the highest value was 18.0 g/dL. The conclusion in this study is that almost all farmers who use pesticides have hemoglobin levels within normal limits. The suggestion for this research is to be able to add a series of complete blood profile examinations such as erythrocytes, hematocrit, leukocytes, and platelets.
Hubungan Antara Kadar Magnesium Serum Dengan HbA1c Pada Penderita Diabetes Mellitus Tipe 2 Di RSUP Fatmawati Jakarta: Correlation between MagnesiumSerum and HbA1c Levels in Patients with Type 2 Diabetes Mellitus at Fatmawati General Hospital Jakarta Prasetyorini, Tri; Lestari, Diah; Putri, Wandira Nur Eka
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6089

Abstract

Diabetes Mellitus (DM) is a collection of symptoms that arise in a person caused by an increase in blood glucose levels due to a progressive decrease in insulin secretion. magnesium (Mg) plays an important role in insulin secretion, insulin binding, and homeostasis. Mg Serum level is an important factor in the etiology and development of macrovascular and microvascular complications in diabetes mellitus patients. HbA1c in DM patients is used to monitor glycemic control and predict the progression of diabetes complications. The purpose of this study was to determine the relationship between serum magnesium and HbA1c levels in patients with type 2 diabetes mellitus. The design of this study was an analytical observational study with a cross-sectional design. The data used are secondary data obtained from patients with type 2 diabetes mellitus at Fatmawati General Hospital for the period 2019 – 2020. The research sample used was 84 data for patients with type 2 diabetes mellitus. Analysis of the data used includes descriptive analysis and then hypothesis testing using the Spearman correlation test. The result of this study showed an association between serum magnesium and HbA1c levels with a value of p = 0,000 (p ≤ 0,05) and r = 0,513. The conclusion obtained in this study is that there is a strong negative pattern relationship between serum magnesium and HbA1c levels in type 2 diabetes mellitus.
Perbedaan Hasil Pewarnaan Hematoxylin Eosin (HE) Pada Histologi Kulit Mencit (Mus Musculus) Berdasarkan Ketebalan Pemotongan 3 Μm, 6 Μm Dan 9 Μm Nazhiifah, Tristania Shofi; Sofyanita, Eko Naning
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 1 (2023): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i1.6090

Abstract

Sectioning is a step that must be passed before staining Hematoxylin Eosin (HE). The aim of this study was to determine differences in the results of Hematoxylin Eosin (HE) staining in mice skin histology (Mus musculus) based on the thickness of the microtome sections of 3 μm, 6 μm, and 9 μm. Experimental research, true experimental research design post test only control group design. The research sample was mice skin preparations (Mus musculus). Primary data collection, cutting of skin preparations using a microtome and staining Hematoxylin Eosin (HE). Field readings were carried out at 400x magnification (40x objective). Data was processed using Kruskal Wallis and Man Whitney. The average value of 6 μm nuclear cutting was 1.44, cytoplasm was 1.15 and color uniformity was 3. The results showed an abnormal distribution, the Kruskal Wallis test (p=0.008) there was a difference in the quality of staining in the 3 μm, 6 μm and 9 μm. The Man Whitney test for the 3 μm and 6 μm microtome cutting groups (p=0.412) showed no difference in the group, the 6 μm and 9 μm microtome cutting groups (p=0.004) there were differences in the group, the 3 μm and 9 μm cutting groups ( p=0.004) there was a difference in the cutting group. The conclusion of this study was that 6 μm microtome cutting produced preparations with better staining quality compared to 3 μm and 9 μm microtome cutting.
Review: Aplikasi Histopatologi Untuk Praktik Forensik: Review: Applications of Histopathology for Forensic Practice Faulana, Arum Fahmi; Empra, Desi Eka Putri; Rahmadian, Desti; Shalihah, Fianicha; Nuriliani, Ardaning; Retnoaji, Bambang; Saragih, Hendry Tri Sakti SG; Rohmah, Zuliyati; Septriani, Nur Indah
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 2 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i2.6582

Abstract

Forensics plays an important role in investigating crimes because it can reveal everything related to a murder case, including the time and cause of the murder. Histopathology is the study of damage to body tissues and organs which can be used to help investigate crimes. The aim of this literature review is to study the role of histopathology applications for forensic practice. The method used is searching journals and books using Google Scholar, ScienceDirect, NCBI, and PubMed with the keywords histopathology, forensics, diagnosis, death, microscopy, disease, staining, and autopsy. The histology approach helps the pathologist make a clear determination on the cause of death. The aim of histopathology is to perfect the macroscopic diagnosis and act as an additional examination in forensic case management. One of the histopathology methods in the forensic field is the paraffin method with hematoxylin eosin staining and special stains, immunohistochemistry, and MITS. The parameters observed with this method are measurements of pulmonary fat embolism; determining the age of myocardial infarction; assessment of liver steatosis, fibrosis, and cirrhosis; determination of the age of thrombus or thromboembolism; and diagnosis of shock with various diseases. The prospect of forensic histopathology is to complement molecular diagnostic methods, so that the combination of molecular and histology methods can play a maximum role in the field of forensic pathology. The conclusion of this literature review is that histopathology can be used to confirm the diagnosis in forensic cases and has good future prospects if combined with other methods such as molecular.

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