Bioscientist : Jurnal Ilmiah Biologi
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
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Profil Creative Thinking Siswa SMA di Lombok : Habits of Mind
Masiah, Masiah;
Firdaus, Laras
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.
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DOI: 10.33394/bioscientist.v11i2.9378
Creative thinking is one of the skills that every individual must master in the 21st century. Each person has a different level of creative thinking. Measurement of creative thinking needs to be carried out so that in the learning process teachers can determine the learning methods and models that will be intensified. The aim of this research is to describe the creative thinking of high school students in Lombok which was accessed through a questionnaire. This research is survey research. The sample determination method is convenience sampling. The respondents involved were 731 students spread across 14 high schools in 3 districts in Lombok. Data were analyzed descriptively with percentages. The research results show that the average creative thinking of high school students in Lombok is in the creative category. This research is part of preliminary research, and for further research this data is verified with a creative test.
Efektivitas Eco Enzyme Limbah Jeruk Manis (Citrus sinensis) sebagai Obat Oles Penyembuhan Luka Sayat
Dzahabiyyah, Qonitah;
Asy’ari, Asy’ari;
Suharti, Peni
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.
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DOI: 10.33394/bioscientist.v11i2.9346
Sweet orange (Citrus sinensis) is a fruit that is much liked by Indonesian people, but from this fruit only the flesh and skin is wasted which eventually becomes waste. Because of that, an alternative solution to this problem is needed, namely Eco Enzyme is made in the form of a multifunctional solution that is used as a wound medicine. The purpose of this study was to determine the differences in the effect of sweet orange (Citrus sinensis) Eco Enzyme concentrations on the healing rate of cuts in mice (Mus musculus). This type of research was experimental with a Completely Randomized Design (CRD) consisting of 7 treatment groups and 4 repetitions, the research data analysis technique used the Kruskal-Wallis and Mann Whitney tests. The results showed that the average percentage of healing rate by applying Eco Enzyme topical medication from sweet orange (Citrus sinensis) waste was most effective on the healing rate of cuts in mice (Mus musculus) with various concentrations, namely P5 (100%) healing rate 100% average healing time 8.25, P4 (80%) healing rate 93.75% average healing time 9.25, P3 (60%) healing rate 87.5% average healing time 9.75, P2 (40%) cure rate 81.25% average healing time 10.25, P1 (20%) healing rate 75% average healing time 10.75, positive control (K+) betadine has a healing rate of 87.5% healing time an average of 10.25, negative control (K-) distilled water has a 50% healing rate healing time beyond day 14. The conclusion obtained is that there is a difference in the effect of orange Eco Enzyme concentration sweet (Citrus sinensis) on the healing rate of cuts in mice (Mus musculus).
Kemampuan Filter Alami Berbasis Kulit Pisang (Musa paradisiaca L.) dalam Penjernihan Air
Sihite, Nadia Okta Kristiana;
Sutarno, Sutarno;
Parlindungan, Deni;
Johan, Henny;
Karyadi, Bhakti
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.
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DOI: 10.33394/bioscientist.v11i2.9161
Waste from male banana peels (Musa paradisiaca Var. Paradisiaca), Ambon banana peels (Musa paradisiaca Var. Sapientum), and kepok banana peels (Musa acuminate L.) can be used as natural water filtration. The content of pectin, galacturonic acid and cellulose in banana peels can absorb heavy metals in water. This research aims to determine the filtration ability of natural waste materials such as kepok banana peels, Ambon banana peels and male banana peels which are most effectively used as water filtration media. This research consists of the sample preparation stage, carbonization stage, activation stage, and trial stage. The method used is an experimental method on a laboratory scale to measure Potential Hydrogen (pH), Total Dissolve Solid (TDS), and the level of clarity in water. The data obtained was tested using the Anova test with a significance level of 5% and the BNT test. Based on the BNT test which shows that male banana peels are not significantly proportional to the TDS of well water. Meanwhile, Ambon banana peel and Kepok banana peel are significantly proportional to TDS, and for Kepok banana peel, Ambon banana peel and male banana peel, it is significantly proportional to the pH of well water, because the significance results are less than 0.05%. From these findings it can be concluded that Kepok banana peels and Ambon banana peels are the most effective water filtration media to use.
Analisis Molekuler Tilapia Lake Virus (TiLV) di Sentra Budidaya Benih Ikan Gurami (Osphronemus goramy) Jawa Timur
Jumria, Andi;
Amin, Muhamad;
Mufasirin, Mufasirin
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.
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DOI: 10.33394/bioscientist.v11i2.9515
Tilapia Lake Virus (TiLV) is a New Emerging Disease that has been defined and included in the WOAH disease list (WOAH, 2022). This virus can infect both freshwater and marine fish, causing high mortality in Ttilapia up to 90%. Reverse transcyptase method - Semi Nested Polymerase Chain Reaction (RT-snPCR) for detection of TiLV in Osphronemus goramy. TiLV was discovered for the first time in Thailand and was also found in Jogjakarta in 2021. Further research is needed to see the damage and genetic variation caused by TiLV in Osphronemus goramy. This study aims to study the genetic variability and homology of TiLV isolates in Osphronemus goramy as definitive hosts in East Java. The results showed clinical symptoms of lethargic fish, decreased appetite, abnormal behavior, reduced reflexes, sluggish movements, darker body color, congestion, thinning fins and exopthalmia. The PCR results showed that the Osphronemus goramy showing clinical symptoms of TiLV infection were all positive. The sequencing results were further identified using the BLAST program, showing the highest similarity of the genetic identity of the sample at 96.8% with TiLV from Israel (Genebank Accession Number NC 029927.1).
Kompatibilitas pada Persilangan Self Anggrek Dendrobium aphyllum
Welsiliana, Welsiliana;
Dewi, Ni Putu Yuni Astriani;
Makin, Florian M. P. R.;
Wiguna, Gede Arya;
Jefriyanto, Wilson
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.
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DOI: 10.33394/bioscientist.v11i2.9376
Indonesia has a very rich biodiversity, one of which is the genetic diversity of orchid species. Preservation and propagation of orchids are always carried out because they have many benefits and the availability of orchid germplasm is always there. Therefore, this research aims to test the compatibility of the Dendrobium aphyllum orchid through self-crossing. The crossing method used is self-crossing, namely crossing flowers in the same flower arrangement. The cross begins by taking 100 flowers, then each flower takes its pollinia and inserts it into the stigma hole of the other flower. After the crossing is carried out, observations are continued once a week and if fruit is successful then observations are carried out once a month. The results of the research showed that the percentage of flowers that fell off was 81% so the number of fruits that were formed until they were physiologically ripe was 8. In accordance with the research data obtained, the success of the cross shows that the level of self-cross compatibility is relatively low namely 8%, and it can be concluded that the nature of the cross is incompatible (crosses below 30%).
Meningkatkan Keterampilan Proses Sains Melalui Model Pembelajaran Problem Based Learning (PBL) pada Materi Biologi
Ramadhan, Ramadhan;
Ningsih, Kurnia;
Supartini, Sri
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.
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DOI: 10.33394/bioscientist.v11i2.8034
This research aimed to enhance the science process skills of the students through the implementation of the Problem Based Learning (PBL) teaching model in biology learning. This type of research was a classroom action research conducted in two learning cycles. Each learning cycle consisted of planning, implementation, observation, and reflection stages. The subjects of this research were 21 students from Class X.G at SMA Negeri 1 Pontianak for the academic year 2022/2023. The research data were obtained through testing techniques using instruments such as pre-test and post-test questions that reflected six aspects of science process skills: observing, interpreting observations, classifying, predicting, communicating, and formulating hypotheses. The data analysis results showed that there was an improvement in the students' science process skills for each measured aspect in both cycle I and cycle II. The average science process skill score for students in cycle I was 62.7, and in cycle II, it was 80.2. Therefore, the research results can be concluded that teaching using the Problem Based Learning (PBL) teaching model can improve students' science process skills in biology learning.
Validitas Petunjuk Praktikum Berbasis POE (Predict, Observe, and Explain)
Sahratullah, Sahratullah;
Seprianingsih, Dewi;
Mardiana, Mardiana
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.
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DOI: 10.33394/bioscientist.v11i2.9438
This research was carried out with the aim of finding out the validity of POE-based practicum instructions (Predict, Observe and Expline) at SMP Negeri 4 Taliwang. This type of research refers to the 4D development model, which consists of four stages, namely Define, Design, Develop, and Disseminate. This research stage was only carried out until the develop stage, namely testing development on a limited scale. This is because the POE (Predict, Observe and Expline) based practicum instructions have not yet reached the Disseminate stage, namely the dissemination stage at the high school level in West Sumbawa Regency. The instrument used in this research was a validation test sheet for POE-based practicum instructions with data collection techniques using the average expert validation score. The results of data analysis show that the development of a POE-based practical manual is valid and very suitable for use. The validation results obtained very high feasibility from the 10 assessment indicators with a value of 87.5%. POE-based practical instructions have been validated by a team of experts, then revised based on suggestions from the validator. As well as the results of the POE-based practicum instructions readability test on 15 indicators with easy criteria with a score of 98.64%. Among the 15 readability test indicators, there were 2 indicators that received hesitant answers from respondents and 3 indicators that received disagreeing answers from respondents. The implications of these practical instructions can be utilized by educators and students in learning in the classroom and outside the classroom, especially in laboratory activities.
Pengaruh Pemberian D-Galaktosa Intraperitoneal terhadap Kadar IL-6 pada Tikus Sprague-Dawley
Manus, Widya Christine;
Pratiwi, Johana Puspasari Dwi;
Ardiyan, Yustina Nuke
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.
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DOI: 10.33394/bioscientist.v11i2.7658
As humans age, there is a decline in the function and physiological mechanisms of the body, which can affect quality of life. Chronic inflammation is one of the characteristics found in the aging process, marked by an increase in proinflammatory cytokines in circulation. IL-6 is one of the cytokines that play a role in chronic inflammation. This study aimed to determine serum IL-6 levels using D-galactose induction in Sprague-Dawley rats. The study used a pure experiment with a post-test only control group design, in which 36 male Sprague-Dawley rats were divided into two groups: control and treatment. The research sample was serum, and the control group received a placebo of 0.9% NaCl at a dose of 400 mg/day/kg intraperitoneally. The treatment group, which underwent aging induction, was given D-galactose at the same dose and route. The treatment was administered for 6 weeks, and serum was collected from blood taken in the 6th week. The results indicated that the administration of D-galactose did not have a significant effect on increasing IL-6 levels.
Uji Aktivitas Sel Kanker Payudara T47D Ekstrak Etil Asetat Daun Glodokan Tiang (Polyalthia longifolia L.) Secara In Vitro
Shofi, Muh.;
Munawaroh, Siti
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.
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DOI: 10.33394/bioscientist.v11i2.9345
Cancer is one of the main causes of death throughout the world. Breast cancer is a non-communicable disease and a type of cancer that is often suffered by women. Breast cancer is a reproductive health problem, both in the world and in Indonesia, which is now a serious concern. Medical treatment of cancer is usually carried out with chemotherapy, surgery and radiotherapy, but the Indonesian people are familiar with and use efficacious plants as an effort to cure various diseases, including cancer. One of them is by using glodokan mast leaves or Polyalthia longifolia L. as natural chemotherapy from biological ingredients. This plant is reported to be high in antioxidant content, so it can be used as an anticancer agent, especially T47D breast cancer. The aim of this research is to examine the compound content and activity of T47D breast cancer cells from P. longifolia leaf extract. This research is basic experimental research, namely by comparing the IC50 value of ethyl acetate extract on young and old leaves. Based on the research results, it is known that the ethyl acetate extract in young and old leaves contains alkaloids, flavonoids, tannins and terpenoids which are useful as anticancer compounds. Cytotoxic activity of ethyl acetate extract of P. longifolia leaves against T47D breast cancer cells with an IC50 value of 253.76 μg/mL on old leaves and young leaves of 220.54 μg/mL with moderate cytotoxicity.
Pengaruh Konsentrasi Crude Enzim Bacillus subtilis terhadap Kadar Gula dan Bioetanol Hasil Fermentasi Kulit Singkong Menggunakan Zymomonas mobilis
Arief Abdillah Nurusman;
Trianik Widyaningrum;
Rifda Khairun Nisa;
Listiatie Budi Utami
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.
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DOI: 10.33394/bioscientist.v11i2.9491
Bioethanol is an environmentally friendly and renewable alternative fuel. The use of alternative fuels is necessary because petroleum fuels make a major contribution to increasing greenhouse gas concentrations, especially CO2. Bioethanol can be obtained from materials containing carbohydrates such as cassava peel which has a high carbohydrate content, namely 4.55%. The aim of this research was to see the effect of the crude enzyme concentration of Bacillus subtilis on the results of sugar and bioethanol content of cassava peel fermentation with Zymomonas mobilis and to find out the crude enzyme concentration that had the greatest influence. This research is an experimental study with the independent variable, namely the concentration of crude cellulase enzyme B. subtilis with concentrations of 0%, 2.5%, 5%, 7.5%, 10%, 12.5%, 15%, and 17.5% , and the dependent variables are sugar content from hydrolysis and bioethanol content from the fermentation process. The data obtained were analyzed using the ANOVA test and Duncan's test. The results of research on the effect of crude Bacillus subtilis cellulase enzyme concentrations of 8 concentrations have an influence on the amount of sugar and bioethanol produced from fermentation of cassava peel with Zymomonas mobilis. Crude Bacillus subtilis cellulase enzyme with a concentration of 17.5% had the greatest influence on sugar and bioethanol levels with a sugar content of 0.37% and a bioethanol content of 1.67%.