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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 80 Documents
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Search results for , issue "Vol. 13 No. 2 (2025): June" : 80 Documents clear
Efek Skarifikasi Air Panas Dengan Variasi Suhu Dan Waktu Pada Perkecambahan Biji Kacang Hijau (Vigna radiata L.) Setiyanto, Afif Eka Rahma; Munawarti, Aminatun
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.12176

Abstract

This study aims to analyze the effect of initial temperature variation and hot water scarification time on the germination of mung bean seeds. Seeds measuring 0.2 ± 0.05 cm in size and dark green in color were scarified using various scarification treatments, namely control (without scarification) (P0), room temperature for 10 minutes (P1), room temperature for 60 minutes (P2), 35°C for 10 minutes (P3), 35°C for 60 minutes (P4), and 55°C for 10 minutes (P5), with a germination period of seven days. The experimental design used was a completely randomized design with four replications, each replication using 25 seeds. The parameters observed were germination percentage, germination rate index, germination rate, radicle length, and plumule length. The best germination percentage and germination speed index were produced by the control (P<0.05). The best germination rate was produced by the P3 temperature treatment. Radicle length and plumule length did not differ significantly (P<0.05) across all treatments. Hot water scarification with varying temperatures and times in this study did not induce better mung bean germination than the control.
Filogenetik Ikan Kuro (Eleutheronema sp.) di Perairan Pesisir Merauke, Papua Selatan Saleky, Dandi; Situmorang, Ferdinand C.; Welliken K., Marius A.; Pangaribuan, Rosa D.
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.13685

Abstract

This research aims to identify kuro fish (Eleutheronema sp.)  collected from the coastal waters of Merauke. Cytochrome Oxidase Subunit I (COI) Gene was amplified by the Polymerase Chain Reaction (PCR) method, using primers FISH F1 (5’-TCA ACC AAC CAC AAA GAC ATT GGC AC-3’) and FISH R1. (5’-TAG ACT TCT GGG TGG CCA AAG AAT CA -3’), while DNA data analysis uses Mega 11 software. Results research shows that DNA sequence obtained were 675 base pairs long with the identification result being Eleutheronema tetradactylum with a similarity level of 99.54 and 99.85% for each sample. Based on phylogenetic analysis and genetic distance, the two DNA sequences analyzed form the same clade with identification results with a genetic distance of 0.001. Analysis of genetic diversity found 2 haplotypes from the two samples. Both the Sample Have a small genetic distance because THEY are collected from the same waters DNA barcoding and phylogenetics can be used to identify species for management and conservation of species.
Micropopagation of Cryptocarya massoy (Oken) Kosterm. Trough Optimizing Callus Induction with Benzylaminopurine (BAP) Supplementation Hanafi, Muhammad; Noli, Zozy Aneloi; Idris, M.; Hany, Iga Permata
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.13852

Abstract

This study aimed to evaluate the effect of BAP concentrations on explant response and callus formation in Massoia. The experiment was arranged in a Completely Randomized Design (CRD) with three BAP concentrations: 0.5 mg/L, 1.0 mg/L, and 1.5 mg/L, each with 24 replications. The culture medium used was Murashige and Skoog (MS), and observations were carried out for 7 days after planting. Parameters observed included the percentage of viable explants, browning, contamination, average callus formation, callus color, and texture. Data were analyzed using analysis of variance (ANOVA) at a 5% significance level. The results showed that 0.5 mg/L BAP yielded the highest explant survival rate (54%), the lowest browning rate (13%), and 35% contamination. Meanwhile, 1.5 mg/L BAP produced the highest average callus formation (0.21) with compact texture and brown coloration. Although not statistically significant, BAP concentrations affected explant viability and callus formation. A concentration of 0.5 mg/L tended to be optimal for maintaining explant viability, while 1.5 mg/L was more effective in inducing callus. These findings provide a preliminary basis for optimizing Massoia tissue culture techniques.
Efektivitas Model PBL Berbantuan Electronic Portfolio Berbasis Web Terhadap Ownership of Learning, Keterampilan Berpikir Kritis, Keterampilan Pemecahan Masalah, dan Hasil Belajar Kognitif Siswa Mutsaqqofa, Amna Roisah; Susilo, Herawati; Prabaningtyas, Sitoresmi
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14096

Abstract

This research aims to determine the effectiveness of the web-based electronic portfolio-assisted PBL model on ownership of learning, critical thinking skills, problem-solving skills, and cognitive learning outcomes. The type of this research is pre-experimental with a one-group pretest-posttest design involving 36 XI IPA students at SMAN 7 Malang.The measurement instruments used include a learning ownership questionnaire, a critical thinking skills test in essay form, a problem-solving skills test in essay form, and a cognitive learning outcomes test in multiple-choice form. Data analysis using the N-gain test to measure the overall effectiveness of learning by looking at the improvement in student scores from pretest and posttest results. The research results show the average N-gain score for ownership of learning (N-gain = 0.3 low), critical thinking skills (N-gain = 0.7 moderate), problem-solving skills (N-gain = 0.6, moderate), and cognitive learning outcomes (N-gain = 0.4 moderate).Thus, it shows that the web-based electronic portfolio-assisted PBL model is an effective teaching strategy to enhance students' ownership of learning, critical thinking skills, problem-solving skills, and cognitive learning outcomes in the learning process.
Pengelolaan Ekowisata Mangrove Berkelanjutan Sebagai Sarana Edukasi dan Pembelajaran IPA di Bale Mangrove Jerowaru Kabupaten Lombok Timur Idrus, Agil Al; Gunada, I Wayan; Idawati, Sri; Nirmala, Siska Ayu; Yahdi, Yahdi; Munib, Munib; Dewi, Earlyna Sinthia
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14164

Abstract

This study aims to explore how ecotourism management involving environmental education can increase community awareness and involvement in mangrove conservation. This research design is descriptive qualitative research. The research sample was determined randomly for visitors to Bale Mangrove Ecotourism. The research instrument used questionnaire sheets and interview guidelines with managers, visitors, and the surrounding community as well as observation sheets of ecotourism practices in the area. The research data consisted of responses from structured questionnaires, interviews and qualitative analysis methods, including thematic coding and content analysis, were used to categorize and interpret the responses. The results showed that mangrove ecotourism in Bale Mangrove not only provides economic benefits to the local community, but also acts as an effective educational medium to increase knowledge about the importance of mangrove conservation. With an approach that involves community empowerment and environmental education, it is expected that the management of this ecotourism can provide a sustainable model that benefits both economically and ecologically.
Perbandingan Metode Isolasi Phosvitin dari Kuning Telur Ayam Buras untuk Karakterisasi Aktivitas Bioaktif Andayani, I Gusti Ayu Sri; Iswaini, Dedy; Depamede, Sulaiman N; Kisworo, Djoko; Bulkaini, Bulkaini; Sriasih, Made
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14304

Abstract

This study aims to compare phosvitin isolation procedures from chicken egg yolk based on purity level, time efficiency, and functional activity. The procedures compared include previously published methods and modified methods developed in this study. Phosvitin was isolated from 10 samples of native chicken eggs collected from farms in the Mataram area using a stratified random sampling approach. The isolation process involved several sequential steps: sedimentation, centrifugation, dialysis, and lyophilization. A comparative analysis of the two procedures was conducted based on protein profile characteristics using SDS-PAGE and Fourier Transform Infrared (FTIR) spectroscopy to identify phosphate group interactions and metal ion binding. SDS-PAGE results showed that both procedures yielded α-phosvitin (35 kDa) and β-phosvitin (45 kDa) protein bands. FTIR spectra confirmed that both phosvitin samples contained high levels of phosphate groups, indicating strong phosphorylation and good protein stability. From a chemical and biological standpoint, the modified procedure showed no qualitative differences compared to the previously established method. However, the modified protocol was approximately 30 minutes faster and did not require a pH adjustment step, making it a simpler and more time-efficient alternative for isolating phosvitin from egg yolk.
Validity of Sinergi Learning Model (Science Process Skills Integration with Local Wisdom and Technology) Fitriani, Herdiyana; Tika, I Nyoman; Artawan, Putu; Arnyana, Ida Bagus Putu; Adnyana, Putu Budi; Citrawathi, Desak Made
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14343

Abstract

This study aims to validate the SINERGI (Science Process Skills Integration with Local Wisdom and Technology) learning model, which is designed to improve students' Science Process Skills (SPS) through the integration of local wisdom and technology. Validation was carried out by involving five education experts to assess the validity of the content and construct of the model. Content validity covers six main aspects, namely supporting theory, syntax, social system, reaction principle, support system, and model implementation, while construct validity covers seven aspects, including components of the learning model. The validation results show that the SINERGI learning model has a high level of validity with most indicators in the very valid category and reliability above 85% based on the Percentage of the Agreement method. This finding confirms that the SINERGI learning model is feasible to be applied in 21st-century science learning, although minor improvements are needed in the reaction principle aspect.
Diversity of Bird Species in The Border City of Pontianak, Indonesia, and Sarawak, Malaysia Agil, Muhamad; Riyadi, Ahmad; Abdillah, Annas Nur; Raihan, Raihan
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14570

Abstract

The island of Kalimantan is an island with a very large area. On this island there are three countries namely Brunei Darussalam, Malaysia and Indonesia. Therefore, there are several areas that are border areas between countries, such as North Kalimantan, Indonesia with Sabah Malaysia, and West Kalimantan, Indonesia with Sarawak, Malaysia. Kalimantan Island is known to have high biodiversity, including a variety of bird species, making it very interesting to explore its species diversity. The purpose of this research is to analyze the diversity of bird species and their conservation status found in the border cities of Pontianak, Indonesia, and Sarawak, Malaysia. The method used in this research is the Point Count Method at each predetermined location. All data obtained were analyzed for their diversity index values and the conservation status of each bird species found was determined based on the International Union for Conservation of Nature and Natural Resources (IUCN). The research results indicate that the bird species diversity index in the border city of Pontianak is 1.795, and Sarawak is 1.70. The research results identified a total of 12 bird species from the two border cities. The bird diversity index value in the border area city of Pontianak is 1.795 and Sarawak is 1.70. The value of the diversity index falls into the moderate category. The conservation status of the 12 bird species found is that one species is classified as Vulnerable (VU) and 11 species are classified as Least Concern (LC).
Frekuensi Kehadiran Arthropoda pada Media Dekomposisi Pelepah Nipah (Nypa fruticans) di Kawasan Mangrove Sungai Kakap Kalimantan Barat Mulyani, Lastyanti; Junardi, Junardi; Kurniatuhadi, Rikhsan
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14651

Abstract

This study aimed to determine the frequency of Arthropod occurrence at each stage of decomposition and to assess its relationship with environmental parameters in the mangrove area of Sungai Kakap. Arthropod sampling was conducted using the hand-collecting method on days 15, 25, 35, and 45 after substrate placement. A total of 24 fronds were randomly positioned vertically at a depth of 20 cm below the soil surface, with six replication across four time-based observation points. Arthropod eggs, larvae, pupae, and imago were collected, sorted, and preserved in 70% alcohol. Specimens were identified directly through morphological observation, and the data obtained were analyzed descriptively. The results showed that the Relative Frequency (RF) of Arthropods consisted of 34 genera, dominated by Amphipoda (Allorchestoides: 75% FR) and Diptera (Bactrocera: 75% FR; Eristalinus: 100% FR; Psychoda: 100% FR). An RF of 100% indicates that these genera were consistently present across all observation points and time periods, suggesting their potentially important role in the decomposition process. Variations in Arthropod presence among genera reflect changes in composition at each decomposition stage. These changes were influenced by environmental conditions, particularly salinity, pH, temperature, as well as organic carbon and nitrogen levels. These findings indicate that certain Arthropods have the potential to serve as biological indicators of decomposition stages in mangrove ecosystems. Further research is recommended to investigate microbial dynamics and changes in organic content throughout the decomposition process.
Diversity and Potential Active Compound of the Sponge-Associated Bacteria from Lemukutan Island, West Kalimantan Indonesia as New Type of Antibiotics Baihaqi, M. Fikri; Nabila, Faika; Triwahyuni, Adelia; Fahira, Annisa Nasywa; Khairillah, Yuyun Nisaul
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14660

Abstract

This study aims to determine the diversity of sponge symbiont bacteria and the effectiveness of active compounds of bacteria that are symbiotic with marine sponges from Lemukutan Island waters. The methods in this study are isolation of sponge symbiont bacteria, characterization of sponge symbiont bacteria, antagonistic tests, activity tests of the best sponge symbiont bacterial isolate extract culture filtrate, and GCMS/MS tests. The results showed that nineteen isolates of sponge symbiont bacteria were successfully isolated from sponge types from Theonella cylindrica and Hyattella intestinalis sponges in Lemukutan waters. Seven isolates of sponge symbiont bacteria are included in the Gram-positive bacteria genre. While the other twelve are included in Gram-negative bacteria. Ten of the nineteen isolates of sponge symbiont bacteria have antibacterial activity for S. aureus. Isolate Sp4 10-6 B, based on the results of antagonistic tests, has an inhibition diameter of 10.28 mm, which is categorized as solid inhibition. Ethyl acetate extract of Sp4 10-6 culture filtrate has a minimum inhibitory concentration value of 0.5% against pathogenic bacteria S.aureus. The active compound with the potential for antibiotic development is the Tetradecane compound, which has the highest peak area of the eight other types of compounds. This shows that the concentration of the compound contained is higher than the other compounds. This compound is included in the aliphatic hydrocarbon and alkane groups.

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