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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,428 Documents
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Respons Pertumbuhan Rimpang Bangle (Zingiber purpureum) Pada Perlakuan PGPR (Plant Growth Promoting Rhizobacteria) Wardana, Singgih Tri; Mangandi, Wulan Fransisca; Harmida, Harmida; Setiawan, Doni
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13142

Abstract

Bangle (Zingiber purpureum Rosc.) is one of the species of the Zingiberaceae family that has medicinal properties. Secondary metabolites contained in bangle rhizomes have biological activity as antioxidants, anti-inflammatory, anticancer, anti-asthma, anti-aging, neuroprotective, antimicrobial. Environmentally friendly technological innovation to increase soil nutrient content and plant growth in sustainable plant cultivation efforts is by utilizing plant growth-promoting rhizobacteria (PGPR). This study aims to determine the effect of PGPR concentration on the growth of bangle rhizomes. This study was conducted at the Physiology and Development Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Sriwijaya University. A completely randomized study design was used with five PGPR concentration treatments (0; 75; 125; 175; and 225 ppm). Data were analyzed by Analysis of Variance (Anova) and Duncan's Multiple Range Test (DMRT). The study showed that PGPR treatment can increase the growth of bangle rhizomes compared to no PGPR treatment. The best results at a PGPR concentration of 175 ppm in increasing the growth of bangle rhizomes.
Potensi Aktivitas Antagonistik Streptomyces dari Rhizosfer Pohon Pule (Alstonia scholaris) sebagai Biokontrol Isrianto, Pramita Laksitarahmi; Wilujeng, Sukian; Marmi, Marmi
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13163

Abstract

Pule plant (Alstonia scholaris) is one of the plants that is often chosen for greening purposes and can be used as a medicinal plant. The ability of Pule plant interaction with microbes is shown from its potential as a source of beneficial microbes, both from the phyllosphere, rhizosphere, and endophytes. The tree rhizosphere microbial ecosystem plays an important role in maintaining tree health and has the potential to produce microorganisms with biocontrol activities. This study aims to evaluate the antagonistic activity of Streptomyces sp isolated from the rhizosphere of Pule trees (Alstonia scholaris) on the ability to inhibit pathogenic bacteria. The research method used disc antagonist test to determine the effectiveness of Streptomyces sp. in inhibiting the growth of pathogenic bacteria, macroscopic and microscopic characterization observations to identify isolates, as well as KOH test and catalase test. The test samples were Streptomyces isolates against Bacillus sp., Fusarium sp., and Aspergillus sp. and the data were analyzed descriptively. The results showed that the isolate Streptomyces sp. has the ability to inhibit the growth of Bacillus sp. and Fusarium sp. In biochemical tests revealed that the isolate is a gram-positive bacterium, based on the results of the KOH test showed that Streptomyces sp. is not slimy (+), while the catalase test produced negative (-) showed no bubbles, meaning Streptomyces sp. does not inhibit the growth of Bacillus sp. and Aspergillus sp.
Pengembangan Lembar Kerja Peserta Didik (LKPD) Berbasis Keterampilan Proses Sains Model Bounded Inquiry Laboratory Pada Materi Bioteknologi Naibaho, Amanda Nurul Aisyah; Khairuna, Khairuna
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2025): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i1.13193

Abstract

This study aims to develop and produce a product in the form of a valid and practical science process skill-based LKPD that can improve students' skills in science process skills. The research methodology used is research and development (R&D) using the 4D development model. Data collection was carried out through interviews, questionnaires, and tests. The data analysis method used is qualitative descriptive analysis. The results of this study indicate that the developed LKPD is feasible to use. Based on the results of observations and interviews, LKPD is able to facilitate students to conduct investigations and play an active role in the learning process so that it can improve the results of students' scientific work. The percentage of effectiveness achieved was 88.625% (very effective) and the level of practicality was 92.5% (very practical). The results of the N-Gain test showed an average N-Gain of 0.5709 (moderate) and a percentage of 57.09% (quite effective). Thus, it can be concluded that the developed LKS is feasible to use in learning activities and can improve students' science process skills.
Korelasi Kadar Total Flavonoid Ekstrak dan Fraksi Polar Kelopak Rosella (Hibiscus sabdariffa L.) Terhadap Aktivitas Antioksidan dengan Metode DPPH (2,2-Difenil-1-Pikrilhidrazil) Sari, Fita; Hidayatul, Fathul; Sariwati, Atmira; Wahyuni, Dwi; Hesturini, Rosa Juwita
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13205

Abstract

Senyawa metabolit sekunder rosella memiliki manfaat kesehatan, seperti hipertensi, diabetes, dan penyakit kardiovaskular. Rosella digunakan sebagai bahan minuman kesehatan, karena senyawa aktifnya seperti antosianin, polisakarida, dan flavonoid. Flavonoid dalam rosella diduga memiliki aktivitas antioksidan dalam menangkal radikal bebas penyebab penyakit. Penelitian ini bertujuan melihat profil fitokimia ekstrak dan fraksi polar kelopak rosella dengan Kromatografi Lapis Tipis (KLT) dan dilanjutkan menguji kadar total flavonoid serta aktivitas antioksidan dengan DPPH (1,1-difenil-2-pikrilhidrazil). Studi ini merupakan penelitian kualitatif dan kuantitatif, untuk mendeteksi profil fitokimia dari ekstrak dan fraksi kelopak rosella dilanjutkan uji kadar total flavonoid dan aktivitas antioksidan. Hasil penelitian menunjukkan bahwa (1) adanya senyawa flavonoid, ditandai bercak warna biru pada plat KLT, (2) uji kadar total flavonoid dari ekstrak menunjukkan sebesar 139,4321 ppm sedangkan fraksi polar 204,8334 ppm, (3) uji aktivitas antioksidan menunjukkan bahwa ekstrak rosella memiliki aktivitas lemah dengan nilai IC50 sebesar 656,4775 ppm, sedangkan fraksi lebih kuat dengan nilai IC50 sebesar 110,1132 ppm. Dengan demikian dapat disimpulkan bahwa ekstrak etanol dan fraksi polar kelopak rosella diduga memiliki senyawa flavonoid yang berperan sebagai antioksidan namun fraksi lebih kuat dibandingkan dengan ekstrak etanol kelopak rosella.
Hedonic Test of Instant Powdered Ginger Drink with Sugar and Sugar-Palm Sugar Combination Jannah, Husnul; Badi'ah, Baiq Arriyadul; Amalia Rachman, Irna Ningsi; Desimal, Iwan
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13207

Abstract

Stress, environmental pollution, radiation, and excessive food processing contribute to the production of free radicals, which are harmful to the body. Antioxidants play a role in preventing free radicals from damaging nucleotides. Ginger contains high levels of antioxidants, making it beneficial for overall health. However, ginger has a short shelf life, and its traditional preparation methods are less preferred by many people. Therefore, ginger is now available in the form of instant drink powders, which can be prepared like tea or coffee. This study is a descriptive study using the hedonic test method. Thirty students were selected as panelists. The panelists evaluated two types of ginger drinks made with different sweeteners: sugar and a combination of sugar and palm sugar (sugar-palm sugar). The parameters assessed in this study were color, aroma, taste, and sweetness level. The results of the hedonic test showed that the ginger drink sweetened with the sugar-palm sugar combination had the highest preference percentages across all parameters: color, aroma, taste, and sweetness level. For color, the "like" preference level achieved a percentage of 40%. For aroma, the "really like" preference level reached 43.33%. Taste received a "really like" preference level of 53.33%, and sweetness achieved a "like" preference level of 36.67%. In conclusion, the panelists preferred the ginger drink sweetened with the sugar-palm sugar combination over the drink sweetened with just sugar. This preference is attributed to the addition of palm sugar, which balances the ginger's strong flavor while retaining its distinct taste. Furthermore, the combination of ginger and palm sugar produces a harmonious blend of color, aroma, taste, and sweetness that complements both ingredients.
Induksi Kalus Krisan (Chrysanthemum morifolium var. reagen pink) dengan Penambahan Naphtalen Acetic Acid (NAA) dan Kinetin Secara In-Vitro Sholehah, Washeilatus; Resmisari, Ruri Siti; Oktafia, Safina; Mumpuni, Siwi Putri
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13217

Abstract

Chrysanthemum morifolium var. reagent pink is an ornamental flower that is widely cultivated in Indonesia. The increasing demand makes it difficult for farmers to fulfill the market. In-vitro tissue culture is an effective solution to multiply chrysanthemum seedlings in bulk and quickly. This study aims to identify the most effective concentration in inducing callus in chrysanthemum plants by combining Naphtalen Acetic Acid (NAA) and kinetin. This study was an experimental research with a completely randomized design (CRD), involving 12 treatments and each treatment was repeated 4 times. There were two treatment factors; NAA concentration (0 mg/l, 0.5 mg/l, 1 mg/l) and kinetin concentration (0 mg/l, 1 mg/l, 2 mg/l, 3 mg/l). Parameters in this study included days to callus appearance, percentage of callus explants, callus wet weight, callus color, and callus texture. Data were analyzed using analysis of variance (ANOVA). The results showed that (1) the fastest callus was formed at 11 HST by giving the treatment of 0 mg/l NAA + 3 mg/l kinetin; (2) the optimal treatment for the percentage of callus explants was obtained by giving 1 mg/l NAA + 1 mg/l kinetin); (3) the optimal treatment of callus wet weight was obtained by giving 1 mg/l NAA + 2 mg/l kinetin; (4) the best callus quality was obtained by giving a combination of NAA 1 mg/l and kinetin 1 mg/l, with a crumbly texture and brownish yellow callus.
Efektivitas Ekstrak Alga Coklat Padina australis dalam Menghambat Pertumbuhan Bacillus cereus dan Escherichia coli Sampulawa, Sriyati; Ode, Asmi; Hulopi, Fauzia; Nirmala, Wa; Bahalwan, Farida; Darmawati, Siti
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13225

Abstract

This study aims to determine the effectiveness of the brown algae extract Padina australis in inhibiting the growth of Bacillus cereus and Eschericia coli bacteria. Samples were taken from the waters of Osi Island, West Seram, Maluku Province as much as 1 kg, the sample was then dried using the sundrying method. The dried sample was then soaked for 24 hours and extracted. The extract results were then tested for phytochemicals and antibacterial activity tests carried out in the Microbiology Laboratory of Pattimura University, Ambon. The results showed that (1) The brown algae extract Padina australis contains secondary metabolites in the form of flavonoids, terpenoids, steroids, saponins and alkaloids; (2) The combination of these various compounds provides a synergistic effect that can increase the effectiveness of antimicrobials in inhibiting the growth of Bacillus cereus and Escherichia coli bacteria; (3) The minimum inhibitory concentration (MIC) of Padina australis algae extract has different abilities in inhibiting test bacteria. The MIC of Bacillus cereus bacteria at a concentration of 10% has an inhibition zone of 7 mm and the MIC of Escherichia coli bacteria at a concentration of 60% has an inhibition zone of 11 mm.
Pemanfaatan Urin Kelinci Sebagai Pupuk Organik Cair untuk Meningkatkan Pertumbuhan dan Hasil Tanaman Cabai Keriting (Capsicum Annuum L.) Hulopi, Fauzia; Kamaruddin, Kamaruddin; Ode, Asmi
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13227

Abstract

The purpose of the study is to determine the optimal concentration of rabbit urine administration (POC Rabbit Urine) to increase the growth and yield of curly chili plants (Capsicum annum L.). This type of research is experimental research. This research was carried out in the Experimental Garden of the Faculty of Agriculture, Unidar Ambon, at an altitude of 150 m above sea level, the average daily temperature is around 27-290C.  The experiment will be carried out from February to May 2024. Variables observed on growth components and plant yield components include, plant height, number of leaves, the number of branches, when flowers appear, the number of harvested fruits, the total weight of the fruit of the plant. Observations are made after the plants are 1 week old after planting. The data obtained was analyzed using a variety fingerprint (F test) at the level of 5%. If the real influence is continued, the Smallest Real Difference Test (BNT) is continued. The results showed that the POC treatment of rabbit urine had a real effect on the control of all observed variables. Rabbit urine concentration of 300 ml/L of water resulted in the highest number of harvested fruits and fruit weight compared to other treatments.
Analisis Hubungan Kemampuan Pelayanan Kesehatan dengan Rate Rujukan Puskesmas di Wilayah Kerja BPJS Kesehatan Cabang Mataram Tahun 2024 Asmara, I Made Adi Putra; Saimi, Saimi; Putri, Findi Dwi Ariestania; Swandayana, Putu Gede Wawan
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13233

Abstract

This study aims to analyze the relationship between healthcare service capacity and referral rates at Puskesmas within BPJS Kesehatan Mataram Branch in 2024. This study used a correlation design with a cross-sectional approach. This design aims to evaluate the relationship between the service capabilities of health centers and referral rates at a certain time. The results of the study showed that Puskesmas with inpatient services tend to have safer referral rates compared to non-inpatient Puskesmas. Non-inpatient Puskesmas often refer more cases due to their limited capacity to manage complex cases at the primary level. The results emphasize the need for increased resources and medical training, especially in non-inpatient Puskesmas, to reduce unnecessary referrals to higher-level hospitals. Strengthening service capacity at Puskesmas is essential to ensure effective primary care and reduce the burden on referral hospitals.
Karakteristik Mutu Pikel Jahe dengan Penambahan Ekstrak Bawang Dayak Anggarkasih, Made Gayatri; Fatimah, Ai Imas Faidoh; Hapsari, Rianti Dyah; Nurwitri, CC; Qalbi, Rainatul
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13242

Abstract

Processing ginger into pickles can provide added economic value and potentially become an export commodity. Ginger pickles are made by soaking ginger slices in a salt solution. Improving the quality of elephant ginger pickles can be done by adding natural dyes to have a pink color like Gari. Dayak onion extract can be used as a natural dye. This research aimed to determine the quality characteristics of ginger pickle with the addition of Dayak onion extract to the soaking medium at 7 dan 14 fermentation days and compare it with a salt solution soaking medium of various concentrations. This study is experimental research that used a Completely Randomized Design consisting of 6 treatments, namely a water-based and a Dayak onion extract-based soaking solution, each made with 5%, 7%, and 9% (w/v) salt concentration. The results showed (1) Adding Dayak Onion extract and variations of salt concentration in elephant ginger pickles had a significant effect on pH, and TPT and had no significant effect on the color and number of lactic acid bacteria); (2) Ginger pickles with a water-based 7% concentration salt solution (P2) most preferred treatment by the panelists.

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