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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,384 Documents
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Keanekaragaman Serangga Pada Tanaman Sorgum Genotip Watar Hammu Witing Walla dan Wattar Hammu ManipaTadda Danu, Glorius Paskan; Jayanti, Ruth Maike
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.17717

Abstract

This study was conducted to evaluate the diversity of insect communities associated with sorghum plants of the genotypes Watar Hammu Witting Walla and Watar Hammu Manippa Tadda. Sampling was carried out using a Z-shaped transect method on both genotypes. Each genotype was represented by five replications, with every replication consisting of five plots, resulting in a total of 25 plots per genotype or 50 plots in total. Within each plot, six Yellow Sticky Traps were placed at representative points following the Z transect pattern to ensure systematic and comprehensive sampling. The traps were installed in the morning at approximately 07:00 a.m. and left in the field until 05:00 p.m. on the same day. After collection, insects adhered to the traps were gathered and stored in clear plastic bags for subsequent identification in the laboratory. The results of the analysis showed that the diversity index of the WH Witting Walla genotype was 1.54, while that of the WH Manippa Tadda genotype was slightly higher at 1.62. The dominance index values were 0.29 and 0.28, respectively, both of which are categorized as moderate. Across both genotypes, 13 insect genera were identified, including Musca, Bactrocera, Phaenicia sericata, Condylostylus longicornis, Bemisia, Bathrogia, Nezara, Camponotus, Abispa, Coleomegilla, Coccinella, Pieris rapae, Spodoptera, and Oxya. Among these, Musca sp. (housefly) and Bemisia sp. (whitefly) were the most dominant species, followed by Spodoptera sp. and Oxya sp. These findings suggest that insect communities on both sorghum genotypes exhibit moderate diversity and balanced dominance, indicating a relatively stable ecosystem.
Microclimate Modification through Water Misting Increase Natural Enemy Insect Richness in a Cocoa Agroecosystem Damayanti, Annisa; Windriyanti, Wiwin; Kusuma, Ramadhani Mahendra
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.17799

Abstract

This study aimed to analyze the influence of abiotic factors on the diversity of natural enemies in cocoa fields. The study was conducted from January to March 2025. Various traps were used, including yellow traps, light traps, pitfall traps, and visual observations. Identification showed that cocoa fields with misting applications had a higher number of natural enemies (1,052 individuals) compared without misting fields (658 individuals). The application of misting resulted in higher diversity indices (H'), richness (R'), and dominance (C'), as well as lower evenness (E') compared to untreated fields. These findings suggest that the misting system creates a more favorable microhabitat for natural enemy diversity, thus potentially strengthening the IPM approach in cocoa cultivation. Interestingly, the analysis showed that temperature had a significance value (sig) > 0.05, indicating that temperature did not show a significant correlation with the increase in the abundance of natural enemies, while humidity has a significance value (sig) < 0.05, which shows that humidity has a significant correlation, although statistically relatively small, with the increase in the abundance of natural enemies.
Pengaruh Model Pembelajaran Kooperatif Tipe Think Pair Share Terhadap Hasil Belajar IPA Biologi Pada Siswa Kelas VIII SMP Negeri 7 Palu Mardiana, Indhira; Bialangi, Mursito S.; Shamdas, Gamar B.N; Bustamin; Agni, Raya; Jamhari, Mohammad
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.17985

Abstract

This study aims to describe the effect of the Think Pair Share (TPS) cooperative learning model on the science learning outcomes of eighth-grade students at SMP Negeri 7 Palu. This study used a quasi-experimental design with a pretest-posttest control group design. The population of this study consisted of all eighth-grade students in six classes, with a total of 166 students. The sample of this study consisted of classes VIII A and VIII B, with a total of 54 students. The sampling technique used was purposive sampling, whereby both classes were selected as sample classes because they were relatively homogeneous in terms of student grades, numbers, and characteristics. The data collection technique was a test. The instrument in this study was a learning outcome test used for pretest and posttest. Instrument testing included validity and reliability tests, while assumption testing included normality and homogeneity tests. Hypothesis testing used an independent samples t-test and learning outcome improvement was tested using an N-Gain test. The results showed that there was a significant effect of the TPS cooperative learning model on biology learning outcomes in grade VIII students at SMP Negeri 7 Palu. The average posttest score for the experimental class was 76, while that for the control class was 39. The N-Gain test results showed a higher increase in learning outcomes in the experimental class with a score of 0.72, which is in the high category. In conclusion, the TPS cooperative learning model has a significant effect on biology learning outcomes.
Persepsi Siswa Terhadap Penerapan Model Pembelajaran Project Based Learning Menggunakan Alat Peraga Pada Materi Sistem Pernapasan Manusia di Kelas XI SMAN 1 Bunobogu Artinya, Indri A.; Bustamin; Rauf, Abd.; Bialangi, Mursito S.; Zainal, Syech; Sabran , Moh.
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18017

Abstract

This study aims to describe students’ perceptions of the implementation of the Project -Based Learning (PjBL) model combined with the use of teaching aids in learning the human respiratory system in Grade XI at SMAN 1 Bunobogu. The study employed a descriptive qualitative approach. The research subjects consisted of three students purposively selected based on different levels of ability. Data were collected through participatory observation, in-depth interviews, supporting questionnaires using a Likert scale, and documentation. Data validity was ensured through source and method triangulation, while data analysis followed the Miles and Huberman model. The results indicate positive perceptions across all three aspects. The cognitive aspect showed a supporting questionnaire score of 3.56 (good category), and the teaching aids effectively transformed abstract concepts into more concrete representations. The affective aspect, with a score of 3.58, reflected increased learning interest and motivation. The psychomotor aspect, with a score of 3.53, contributed to the development of practical skills, creativity, and teamwork.
Efektivitas Monitoring EWS Terintegrasi Berbasis Elektronik Terhadap Deteksi Risiko Mortality di RS Bendan Kota Pekalongan Himawati; Pohan, Vivi Yosafianti; Hartiti, Tri; Masrukhi; Armiyati, Yunie
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18115

Abstract

This study aimed to determine the effectiveness of an integrated electronic-based Early Warning Score (EWS), known as TeBar, compared with a paper-based EWS form in detecting mortality risk at Bendan Regional General Hospital, Pekalongan City. A quasi-experimental study with a pretest–posttest control group design was employed. The sample consisted of 60 inpatient ward nurses selected using purposive sampling. The inclusion criteria were staff nurses working in the Jlamprang and Truntum wards, while nurse managers and nurses on leave, permission, or ward transfer during the study period were excluded. The intervention involved monitoring patients using the electronic integrated EWS (TeBar) in the intervention group, whereas the control group used the conventional paper-based EWS form. Data were collected using web-based observation sheets. Statistical analysis was performed using the Wilcoxon Signed Rank Test and the Mann–Whitney U Test. The results showed that before implementing the electronic EWS, most mortality risk detections in the intervention group were inaccurate (80%); however, after implementation, all detections were accurate (100%). In the control group, mortality risk detection using the paper-based EWS remained largely inaccurate both before (66.7%) and after (60%) the intervention. The Wilcoxon test revealed a significant difference in mortality risk detection before and after the use of the electronic EWS (TeBar) in the intervention group (p = 0.000), whereas no significant difference was found in the control group using the paper-based EWS (p = 0.346). Furthermore, the Mann–Whitney U test indicated a significant effect of electronic EWS (TeBar) monitoring on mortality risk detection compared to the paper-based EWS (p = 0.000). In conclusion, the electronic EWS (TeBar) is effective in detecting early mortality risk in inpatient wards at Bendan Regional General Hospital, Pekalongan. The use of electronic EWS improves the accuracy of clinical documentation, accelerates clinical decision-making, and enhances patient safety as part of efforts to improve the quality of nursing care.
Isolation and Characterization of Endophytic Bacteria from Curcuma Aeruginosa Roxb As Antibacterial Candidates Againts Pneumonia Utami, Arti Wahyu; Rezaldi, Firman; Ginaris, Rifkarosita Putri; Nabilla, Jihan; Bramantyo; Setiawati, Puput; Fatikha, Zulfania Shandra; Pujiastuti, Nanik; Wahyuni, Dwi
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18148

Abstract

This study aimed to isolate and characterize endophytic bacteria from black turmeric rhizomes (Curcuma aeruginosa Roxb) and test their potential as antibacterial agents against Streptococcus pneumoniae. This experimental research began with the isolation of endophytic bacteria from surface-sterilized black turmeric rhizomes. The obtained pure isolates were characterized macroscopically (colony shape, margin, elevation, pigment) and microscopically (cell shape, Gram staining). The growth curve of potential isolates was determined, and antibacterial activity was tested using the disc diffusion method against S. pneumoniae, with ciprofloxacin as a positive control. Six endophytic bacterial isolates (PS1, PS2, PS3, PS4, PS5, PS6) were successfully isolated, showing variations in colony morphology, and all were classified as Gram-negative bacilli. All isolates exhibited strong antibacterial activity against Streptococus pneumoniae, with inhibition zone diameters ranging from 15.3 mm to 20.6 mm. Black turmeric rhizomes (Curcuma aeruginosa Roxb) harbor diverse endophytic bacteria with significant potential as antibacterial agents against the pneumonia pathogen, Streptococcus pneumoniae.
Pengaruh Pemberian Nanopartikel TiO₂ pada Berbagai Konsentrasi terhadap Perubahan Morfologi dan Biomassa Kalus Ginseng Jawa (Talinum paniculatum) secara In Vitro Maulana, Zakiy; Noli, Zozy Aneloi; Suwirmen; Fajrisani, Syifa; Putri, Suci Indah
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18160

Abstract

This study aims to analyze the effect of titanium dioxide (TiO₂) nanoparticle concentrations on the characteristics of Talinum paniculatum (Java ginseng) callus. The experiment was conducted using a completely randomized design with six TiO₂ concentration treatments (0, 1, 2, 3, 4, and 5 ppm) over a 14-day incubation period. The observed parameters included callus morphology (color and texture), fresh weight, and dry weight. Fresh and dry weight data were analyzed using one-way analysis of variance (ANOVA) followed by Duncan’s New Multiple Range Test (DNMRT), while callus color and texture were analyzed descriptively. The results showed that the addition of TiO₂ nanoparticles induced changes in callus color ranging from yellow to yellow-reddish, and the resulting callus exhibited a compact texture. TiO₂ nanoparticles up to a concentration of 5 ppm did not show negative effects on either fresh or dry callus weight. These findings indicate that TiO₂ nanoparticles at the tested concentrations play a role in modifying the visual characteristics of callus without affecting biomass accumulation. Therefore, TiO₂ nanoparticles have potential application in enhancing secondary metabolite production.
Analisis In Silico Filogenetik Coffea sp. Berdasarkan Gen matK dan rbcL: Dasar Pemuliaan Profil Sensorik Rizki, Firnanda Ayu; Isnawati
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18204

Abstract

This study aimed to analyze phylogenetic relationships, genetic distances, and the potential for successful hybridization based on concatenated matK and rbcL sequences. The study was conducted in silico on 11 coffee species obtained from the NCBI GenBank database. Sequence data were analyzed using bioinformatics tools for alignment, concatenated sequences, phylogenetic reconstruction, and genetic distance calculation. Phylogenetics produced a stable and accurate topology. Species showed three categories of hybridization compatibility based on genetic distance values; high ensured gene introgression stability, moderate produced heterotic hybrids, and low risked incompatibility and genetic barriers. The study concluded that species with low genetic distance and close kinship have the potential for successful hybridization. The integration of phylogenetics and genetic distance based on matK and rbcL sequences proved effective in mapping interspecies compatibility oriented towards sensory profile development. These findings provide a strategic foundation for the efficiency of modern coffee breeding programs with superior taste and aroma characteristics.
Pengaruh Aplikasi Trichoderma sp. dan Pupuk Organik Kotoran Ayam Terhadap Pertumbuhan Bawang Merah (Allium Ascolonicum L) Nababan, Nurmaini; Rahmadina
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18268

Abstract

This study aims to determine the effect of chicken manure and Trichoderma sp. on the growth and health of shallots (Allium ascalonicum L.). The study was conducted in June–July 2025 at the Greenhouse of the State Islamic University of North Sumatra using a factorial randomized block design (RAK) with two factors. The first factor was the dose of chicken manure (P0 = 0 g, P1 = 100 g, P2 = 120 g, P3 = 140 g), and the second factor was the dose of Trichoderma sp. (T0 = 0 g, T1 = 25 g, T2 = 30 g, T3 = 35 g). The two factors were combined into 16 treatments with three replicates. The parameters observed included plant height, number of leaves, number of wilted leaves, root length, fresh weight, and dry weight of plants. The data were analyzed using ANOVA and followed by DMRT test at the 5% level. The results showed that chicken manure and Trichoderma sp. had a significant effect on plant height, number of leaves, fresh weight, dry weight, and were able to suppress leaf wilting symptoms, but had no significant effect on root length. The combination of treatments yielded the best results compared to single treatments, especially at chicken manure doses P2–P3 and Trichoderma sp. T3. Thus, the application of chicken manure combined with Trichoderma sp. has the potential to optimize shallot growth in an environmentally friendly manner.
Optimasi Kombinasi Ramuan Etnomedisin Penumbuh Rambut Nusa Tenggara Timur Menggunakan Orthogonal Stimulus Response Method Pada Kepadatan Rambut Mencit (Mus musculus) Taek, Maximus M.; Megawati, Dewi Sinta; Subiyakto, Erlangga Sufi; Khoirunnisa, Zahra; Maulina, Novia; Muslikh, Faisal Akhmal; Ma’arif, Burhan
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18301

Abstract

This study aimed to optimize a traditional ethnomedicinal formulation from East Nusa Tenggara (RENTT) as a natural hair-growth candidate and to evaluate its antioxidant activity and biological effectiveness using an alopecia model. A true experimental design was applied using the Taguchi–Orthogonal Stimulus Response Method (OSRM) to determine the optimal combination of five plant extracts, namely Aleurites moluccanus, Hibiscus rosa-sinensis, Ficus benjamina, Polyscias scutellaria, and Hibiscus tiliaceus. In vitro optimization was conducted using the DPPH radical scavenging assay with a Taguchi L27 orthogonal array design. The optimal formulation was subsequently evaluated in vivo using male mice (Mus musculus) with croton oil–induced alopecia. Hair growth effectiveness was assessed based on hair density percentage through histomorphometric analysis with hematoxylin–eosin staining. The results demonstrated that the optimal RENTT formulation exhibited very strong antioxidant activity with an IC₅₀ value of 27.46 ppm. Taguchi analysis identified F. benjamina as the most influential factor in determining formulation performance. In vivo evaluation showed that the RENTT-treated group achieved the highest hair density (49.45 ± 4.70), which was significantly higher than that of the positive control, negative control, and normal control groups. In conclusion, the optimized RENTT formulation demonstrates strong antioxidant properties and a significant stimulatory effect on hair growth, indicating its promising potential as a natural cosmeceutical product based on the local wisdom of East Nusa Tenggara.

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