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INDONESIA
journal of Basic Medical Veterinary
Published by Universitas Airlangga
ISSN : 23026820     EISSN : 27749959     DOI : https://dx.doi.org/10.20473
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary
This journal published original articles, review articles, and case studies in Indonesian or English, in the scope of JBMV has a broad coverage of relevant topics across veterinary basic medical sciences which includes: preclinical and paraclinical disciplines like Anatomy, Physiology, Biochemistry, Pathology, Microbiology, Pharmacology and Medical Education.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 114 Documents
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UJI PENETAPAN STABILITAS RETENTION TIME Megestrole acetate DALAM ELUENT MOBILE PHASE MENGGUNAKAN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Cholifah, Siti Chusnul; Lazuardi, Mochamad; Rahardjo, Dadik; Maslachah, Lilik; Sukmanadi, Mohammad; Kurnijasanti, Rochmah
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21093

Abstract

The aim of this research was to determine the level of stability of Megestrole acetate-retention time in storage period for six, eight and 12 hours using High Performance Liquid Chromatography (HPLC). The research method used posttest-only control group design by using three treatments and six repetitions. The three repetitions consist into six hours, eight hours and 12 hours. The data were obtained analyzed by Summery Independent T-Test with SPSS 24 for windows. The result showed six hours retention time of Megestrole acetate is stable and eight hours treatment and 12 hours treatment are not stable there is one unstable point of 12 hours treatment that indicates the substance is break down. Based on those result, it could be concluded that the storage time of Megestrole acetate in Eluent Mobile Phase began to show unstable at eight hours of storage.
KEJADIAN KAWIN BERULANG PADA SAPI POTONG BETINA PERANAKAN LIMOSIN DAN SIMENTAL DI KECAMATAN WONOSALAM KABUPATEN JOMBANG PERIODE 2015 - 2018 Subagio, Muharam; Triana, Indah Norma; Poernomo, Bambang; Wurlina, Wurlina; Srianto, Pudji; Utomo, Budi
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20412

Abstract

Repeat Breeding is an adult cattle which have normal estrous cycle and clear signs of estrous but did not get pregnant when it got breed by natural mating or artificial insemination. The main purposes of this study is to know the number of repeat breeding cases in beef cattle in regency Jombang period of 2015-2018. The metode of the study is survey which collected by recording and survey. Were analyzed though Normality Test (Kolmogorov-Smirnov Test) and Homogenity Test and followed by T Test. Questionnaire test result processed using Kendall's Tau-b Correlation Test. Study result showed that repeat breeding cases fluctuate significantly per year and the factors affecting fertilization failure and early embrionic death, hormonal, infection, and management factors.   
PENGARUH PEMBERIAN EKSTRAK SAMBILOTO (Andrographis Paniculata) TERHADAP GAMBARAN HISTOPATOLOGI GINJAL YANG DIINDUKSI GENTAMISIN PADA TIKUS (Rattus norvegicus) Nugrahani, Anggi Dwi; Nangoi, Lianny; Meles, Dewa Ketut; Widiyatno, Thomas Valentinus; Hamid, Iwan Sahrial; Santoso, Kuncoro Puguh
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19989

Abstract

This research was aimed to know the effect of leaves extract of sambiloto as a nephroprotector on renal histopathology features induced by gentamicin in white rats (Rattus norvegicus). The effect of leaves extract of sambiloto was tested in 25 rats. Twenty five male white rat were randomly divided into five groups; K- as negative control was given carboxy methyl cellulosa in 15 days, P0 was given carboxyl methyl cellulosa in 15 days, P1 was given leaves extract of sambiloto of 177 mg/kg bw/day/po in 15 days, P2 was given leaves extract of sambiloto of 189 mg/kg bw/day/po in 15 days, P3 was given leaves extract of sambiloto of 239,5 mg/kg bw/day/po in 15 days. On the 15 day of experimental, P0, P1, P2, P3 was given 200 mg/kg bw of gentamicin. Gentamicin solutions on P0, P1, P2 and P3 treated through intramuscular injection. After 24 hours of gentamicin induced, take the rats renal by laparotomy and then making preparations histopathology with staining Haematoxylin-Eosin (HE). Based on the result of Kruskall-Wallis statistical analysis on renal histopathology showed this research that the treatment have significant difference (p < 0,05). The leaves extract of sambiloto treated group (P3) gave the best value on the renal morfology, it gave a good effects in preventing nephrotoxicity in white rats.
PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK (Musa acuminata) TERHADAP GAMBARAN HISTOPATOLOGI BRONKUS DAN VENA PULMONALIS MENCIT (Mus musculus) JANTAN YANG DIPAPAR ASAP ROKOK Jeanita, Winny; Meles, Dewa Ketut; WIdjiati, Widjiati; Hamid, Iwan Sahrial; Luqman, Epy Muhammad; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21089

Abstract

This research aimed to attest the effect of Kepok banana (Musa acuminata) peel extract on the histopathology of bronchus and pulmonary veins in male mice (Mus musculus) exposed by cigarette smoke. The 24 experimental male mice were divided into six groups with each treatment having four replicate. All the groups, except K- as a control group, was treated with cigarette smoke for 14 days. After that, K- and K+ was treated with CMC-Na 1%, KO was treated with ascorbic acid 13 mg/kgBW, P1 was treated with Kepok banana peel extract 14 mg/kgBW, P2 was treated with Kepok banana peel extract 28 mg/kgBW, and P3 was treated with Kepok banana peel extract 56 mg/kgBW for 14 days. The data of the thickness of bronchial epithelium and the diameter of pulmonary veins were analyzed using ANOVA (Analysis of Varience) with significance p<0,05 and continued with Post- hoc Tukey. The result of the thickness of bronchial epithelium showed that K+ had the highest thickening (431,83 + 9,71 µm) and significant differences (P<0,05) with all the groups. The result of P3 showed the lowest thickening of bronchial epithelium (170,84 + 2,70 µm). The result of pulmonary veins diameter showed that K+ had the narrowest diameter (525,64 + 16,61 µm) and significant differences (p<0,05) with all the groups. The result of P3 showed the lowest diameter narrowing of pulmonary veins (718,10 + 9,64 µm). The conclussion of this research are Kepok banana peel extract able to decreased the thickness of bronchial epithelium and the narrowing of pulmonary veins diameter in male mice with effective dose 28 mg/ kgBW.
KEMAMPUAN MALARISIDAL NANOPARTIKEL EKSTRAK DAUN JUWET (Syzygium cumini) SEBAGAI TERAPI AJUVAN PADA HISTOPATOLOGI HATI MENCIT (Mus musculus) PENDERITA MALARIA Pujiyanto, Mahendra; Maslachah, Lilik; Triakoso, Nusdianto; Lazuardi, Mochamad; Anwar, Chairul; Legowo, Djoko
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21094

Abstract

The aimed of this study is to know malaricidal ability of nanoparticle juwet (Syzygium cumini) leaf extract as adjuvant therapy on liver histopathological of mice (Mus musculus) which infected by Plasmodium berghei. This research uses 30 mice which are divided into six treatment groups namely K0 (not infected without treated), K+ (infected without treated), K1 (infected and treated with chloroquin), K2 (infected and treated with juwet leaf extract), P1 (infected and treated with juwet leaf extract nanoparticle) and P2 (infected and combination treated with chloroquine and juwet leaf extract nanoparticle) which infected by 1x105 in 0.2 ml of Plasmodium berghei. The data were analyzed by Kruskal Wallis using SPSS and followed with Mann-Whitney test. This research result showed there was significant difference (p < 0.05) between K1 and P2, but did not showed significant difference (p > 0.05) with K+, K2 and P1. Based on this research, it could be concluded that nanoparticle juwet (Syzygium cumini) leaf extract as adjuvant therapy estimated can increase malaricidal ability in degeneration, necrosis, portal inflammation, Kupffer cell hyperplasia, sinusoidal congestion, and hemosiderosis in mice liver which infected by Plasmodium berghei.
IDENTIFIKASI BAKTERI Escherichia coli PENGHASIL Extended Spectrum β-Lactamase DARI SWAB RECTAL SAPI PERAH MENGGUNAKAN METODE VITEK-2 DI KUD TANI WILIS SENDANG KABUPATEN TULUNGAGUNG Syah Putra, Akyun Rozaqi; Effendi, Mustofa Helmi; Koesdarto, Setiawan; Suwarno, Suwarno; Tyasningsih, Wiwik; Soelih Estoepangestie, Agnes Theresia
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20414

Abstract

Antibiotic resistance in animals and humans has become a global problem that needs attention. The use of antibiotics in inappropriate on food-producing animals can lead to resistance many of the pathogenic bacteria to the various types of antibiotics, one of which is the Escherichia coli (E. coli) which produces extended spectrum β-lactamase (ESBL). The aim of this study was to isolate and identified ESBL- E. coli isolate from dairy cow rectal swabs in Sendang, Tulungagung district using the Vitek-2 method. The number of rectal swab samples used in the present study was 50. The result of the study showed that from all of the samples could be isolated and indentified E. coli, based on the colony characteristics on EMBA and biochemical test. Based on the double disc synergy test method using antibiotic disc amoxicylyn-clavulanate, ceftriaxone, aztreonam, ceftazidime and cefotaxime, 10 isolates could be identify els ESBL- E. coli. furthermore 3 out of 10 isolates DDST positives were confirmed ESBL- E. coli using Vitek-2 method.
DESCRIPTION OF HEMATOLOGICAL VALUE IN LEOPARD GECKO (Eublepharis macularius) Wiranata, Try Ardhi; Widjiati, Widjiati; Wahyuni, Retno Sri; Hidajati, Nove; Poetranto, Emmanuel Djoko; Yuliani, M. Gandul Atik
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19990

Abstract

The aim of this research was to explore the hematological value of leopard gecko (Eublepharis macularius). Total of 20 samples were examined, its consists of 10 males and 10 females, blood samples were taken from vena ventral abdominal and deposited into EDTA tubes, fresh blood leaves little to make blood smear, total blood examination conducted with automatic hematological analyzer ABX MICROS 60 and for total leukocyte and differential leukocyte count used standard manual method. The mean result of hematological value of leopard gecko in this research are WBC (x103/mm3) 7,12 male, 8,09 female, Lymphocyte (%) 46,2 male, 38,4 female, Monocyte (%) 17 male, 17,3 female, Heterophil (%) 18,3 male, 21,8 female, Eosinophil (%) 10,8 male, 13,9 female, Basofil (%) 7,7 male, 8,6 female, RBC (106/mm3) 0,64 male, 0,65 female, HGB (g/dl) 8,95 male, 9,46 female, Hematocrit (%) 10,55 male and 9,26 female. In all parameters showed that hematology value of male gecko leopard was lower than female gecko except on two parameters which are hematocrit and lymphocyte which showed male gecko has the higher value than females, but the hematology value difference of both sexes leopard gecko is not significant except in heterophil value. 
PENETAPAN RETENTION TIME PROGESTERON DALAM PELARUT ELUEN PHASE MOBILE MENGGUNAKAN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY Chandra, Evania Haris; Lazuardi, Mochamad; Sarudji, Suryanie; Maslachah, Lilik; Sukmanadi, Mohammad; Utama, Suzanita
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21090

Abstract

The aim of this research was to determine the level of stability of Progesterone-retention time on Eluen Phase Mobile (EPM) solution in storage period for six, eight and 12 hours using High Performance Liquid Chromatography (HPLC). The research method used posttest-only control group design by using three treatments and six repetitions. The three repetitions consist into six hours, eight hours and 12 hours. The data were obtained analyzed by Summery Independent T-Test with SPSS 24 for windows. The results showed that the progesterone treatment in EPM solvents with six-hour storage (P1) and eight hours (P2) retention time progesterone are stable but the progesterone treatment in EPM solvent with 12-hour storage (P3) showed an unstable due to storage time. Besides that there are irrelevant peak which show that many pure molecular fractions are separated in the stationary phase so that the molecular structure of the substance begins to break down. Based on these results, it can be concluded that the storage time of Progesterone in Eluen Phase Mobile began to be unstable at 12 hours of storage. 
THE EFFECT OF POLYPROPYLENE PLASTIC RESIDUE ON HEATED PALM OIL AGAINST HISTOPATHOLOGICAL CHANGES OF SMALL INTESTINE ON MALE WHITE RAT (Rattus norvegicus) WISTAR STRAIN Amaris, Aulia Puspa; Yustinasari, Lita Rakhma; Legowo, Djoko; Plumeriastuti, Hani; Anwar, Chairul; Hidajati, Nove
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20409

Abstract

This study was conducted to investigate the effect of polypropylene residue on heated palm oil against histopathological changes of small intestine on male white rat (Rattus norvegicus) wistar strain. Eighteen male white rats divided into three groups and were administered via peroral gavage with different treatments for five weeks. The treatment were Control (84 grams palm oil), P1 (84 grams palm oil with 2.3 grams plastic), and P2 (84 grams palm oil with 6.81 grams plastic). The histopathological changes of small intestine were examined by using Barthel Scoring method was analysed using Kruskal Wallis and continued with Mann-Whitney test. The result showed that were significant difference (p<0.05) between treatment groups. The worse damage of polypropylene plastic residue was 6.81 grams.
POTENSI KANDUNGAN ANTIOKSIDAN RUMPUT KEBAR (Biophytum Petersianum Klotzsch) TERHADAP APOPTOSIS DAN GAMBARAN HISTOPATOLOGIS TESTIS PADA MENCIT (MUS MUSCULUS) YANG DIPAPAR 2,3,7,8-Tetrachlorodibenzo-p-dioxin Dewita, Dewita; Widjiati, Widjiati; Rimayanti, Rimayanti; Suprayogi, Tri Wahyu; Hariadi, Mas'ud; Plumeriastuti, Hani
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19986

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is the most toxic compound in the dioxin group. This compound is a pollutant for the environment and very harmful to human health and enter the body through the mucous membranes in the mouth and the respiratory tract and can be transmitted through the placenta and lactation. The aim of this study was to know the antioxidantpotency of to resolve reproduction disturbance caused by TCDD exposure.Thirty Balb/C male mice were divided into five different groups, the negative control group, a positive control group exposed to TCDD at a dose of 7 μg/kg BW, P1 group of groups exposed to TCDD doses of 7 μg / kg BW and given Biophytum petersianum extract 0.05mg/gBB/day, group P2 group exposed to TCDD doses of 7μg/kgBW and given 0.080mg/gBB/day, and group P3 were exposed to TCDD dose of 7μg/kgBB and given the extract of Biophytum petersianum 0.135mg/gBB/day during day 2 to day 55. On the 56th day the mice were sacrificed and apoptotic examination and spermatogenic cell histopathological features were performed on the testis. The results showed that: P2 (0,433 ± 0,497; p<0,05) and P3 (0,200 ± 0,000 p<0,05) groups were the most effective group in decreasing spermatogenic cell apoptosis compared to positive control group (2,933 ± 1,5832 p<0,05). The Johnsen score result showed that P2 (9,400 ± 0,420) and P3 (9,800 ± 0,253) groups improving the histopathologic picture of spermatogenic cells in seminiferous tubules compared to positive control group (7,20 ± 0,400) p<0,05. Conclusion of this study were Biophytum petersianum is effective to solve reproduction disturbances caused by exposure of TCDD and the P3 group is the most effective group.

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