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Journal of Experimental and Clinical Pharmacy (JECP)
Published by Politeknik Kesehatan Kementerian Kesehatan Gorontalo
ISSN : -     EISSN : 27751368     DOI : https://doi.org/10.52365/JECP
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health
Journal of Experimental and Clinical Pharmacy (JECP) merupakan jurnal yang issue tiap 6 bulan sekali (Februari-Agustus) dalam bidang farmasi eksperimen dan farmasi klinik secara nasional. Penelitian eksperimental dalam jurnal ini mencakup penelitian pengembangan obat dan makanan secara in vivo, in vitro, in silico, dan formulasi serta pemeriksaan mutu makanan. Sementara itu, penelitian klinis mencakup uji klinik, case report, case series, cohort, dan crossectional dari penggunaan obat obatan dan kosmetika. Kami juga menerima artikel penelitian mengenai farmasi komunitas dan entreprenuer pharmacy (Pharmaprenuer). Selain artikel penelitian, kami juga menerima karya dalam bentuk review artikel, mini review dan Short Communication. Jurnal ini adalah jurnal akses terbuka Jurnal ini adalah jurnal akses terbukadan tidak memungut biaya publikasi untuk berlangganan, pengiriman, dan penerbitan jurnal.
Arjuna Subject : Pharmacology, Toxicology and Pharmaceutics - Pharmacology, Toxicology and Pharmaceutics (Lain-Lain)
Articles 82 Documents
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Uji Mutu Fisik dan Penetapan Kadar Magnesium Hidroksida pada Tablet Kunyah Antasida dengan Nomor Batch Berbeda Rahmaningrum, Puji Laili; Saputra, Sony Andika
Journal of Experimental and Clinical Pharmacy (JECP) Vol 3, No 2 (2023): August 2023
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v3i2.462

Abstract

Antasida merupakan obat penetral asam lambung yang digunakan untuk membantu mengatasi gangguan pencernaan penderita gastritis. Uji mutu fisik dan penetapan kadar dijadikan parameter untuk mengetahui mutu suatu obat. Penelitian ini bertujuan untuk memastikan mutu fisik dan kadar magnesium hidroksida pada obat antasida bermerek dengan nomor batch berbeda sesuai dengan spesifikasi. Uji mutu fisik yang dilakukan meliputi uji keseragaman ukuran, keseragaman bobot, kerapuhan, dan kekerasan tablet, sedangkan metode yang digunakan pada penetapan kadar magnesium hidroksida adalah spektrofotometri UV-Vis dengan pengompleks Eriochrome Black Tea (EBT). Hasil uji mutu fisik telah memenuhi persyaratan yang ditetapkan Farmakope Indonesia Edisi III dan non Farmakope. Hasil optimasi penentuan panjang gelombang maksimum dan operating time didapatkan pada 408 nm dengan operating time stabil pada menit ke 5-15. Hasil dari penetapan kadar pada masing-masing sampel uji diperoleh rerata sebesar 94,844% dan 98,282%. Kadar tersebut memenuhi persyaratan pada Farmakope Indonesia Edisi VI. Hasil uji statistik Independent T-Test dengan  = 0,05 menunjukkan tidak ada perbedaan yang signifikan.Antacids are stomach acid-neutralizing drugs that are used to help treat digestive disorders in gastritis sufferers. Physical quality tests and assays are used as parameters to determine the quality of a drug. This study aims to ensure the physical quality and levels of magnesium hydroxide in branded antacid drugs with different batch numbers according to specifications. Physical quality tests carried out included tests for size uniformity, weight uniformity, friability, and tablet hardness, while the method used to determine magnesium hydroxide levels was UV-Vis spectrophotometry with Eriochrome Black Tea (EBT) complex. The results of the physical quality test have met the requirements set by the Indonesian Pharmacopoeia Edition III and non-Pharmacopoeia. Optimization results for determining the maximum wavelength and operating time were obtained at 408 nm with a stable operating time of 5–15 minutes. The results of the assay in each test sample obtained an average of 94.844% and 98.282%, respectively. These levels meet the requirements of the Indonesian Pharmacopoeia, Edition VI. Independent T-Test statistical test results with α = 0.05 showed no significant difference.
Isolasi dan Aktivitas Antibakteri Fungi Endofit Daun Miana (Coleus scutellarioides (L.) Benth) terhadap Propionibacterium acne dan Pseudomonas aeruginosa Novita, Novita; Pakadang, Sesilia Rante; Arisanty, Arisanty
Journal of Experimental and Clinical Pharmacy (JECP) Vol 3, No 2 (2023): August 2023
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v3i2.537

Abstract

Daun miana (Coleus scutellarioides (L.) Benth) mempunyai banyak jenis khasiat yang difungsikan menjadi obat, penurunan demam, batuk, influenza, penetralisir racun, antiseptik maupun yang lainnya. Bagian tanaman yang difungsikan menjadi obat yaitu bagian daun. Tujuan penelitian ini untuk mengetahui aktivitas antibakteri isolat fungi endofit daun pada Propionibacterium acne dan Pseudomonas aeruginosa, jenis penelitian yang digunakan adalah eksperimental. Adapun metode pengujian dari penelitian yang dilakukan dengan berupa isolasi dan pemurnian fungi endofit dari daun miana, identifikasi fungi daun miana penghasil bakteri, pengujian diameter zona hambat fungi endofit daun miana terhadap P. acne dan P. aeruginosa dengan metode difusi menggunakan teknik sumuran. Hasil penelitian diperoleh 4 isolat yang digunakan Mukor sp, (putih), Aspergillius niger (hitam), Aspergillius fumigatus (hijau), Rhzipus sp, (abu-abu). Isolat fungi endofit dari daun miana memiliki aktivitas antibakteri pada pertumbuhan P. acne dan P. aeruginosa.Miana leaves (Coleus scutellarioides (L.) Benth) have many types of properties that are used as medicine, including reducing fever, cough, influenza, neutralizing poisons, antiseptics, and others. The part of the plant that is used as medicine is the leaf. The purpose of this study was to determine the antibacterial activity of leaf endophytic fungal isolates on Propionibacterium acne and Pseudomonas aeruginosa. The type of research used was experimental. The testing methods of the research were isolation and purification of endophytic fungi from miana leaves, identification of miana leaf fungi-producing bacteria, and testing the diameter of the inhibition zone of miana leaf endophytic fungi against P. acne and P. aeruginosa by diffusion method using the well technique. The results obtained were four isolates: Mukor sp. (white), Aspergillius niger (black), Aspergillius fumigatus (green), and Rhzipus sp. (gray). Endophytic fungi isolated from miana leaves have antibacterial activity against the growth of P. acne and P. aeruginosa.
Analisis Kemoterapi pada Pasien Tn. “AM” dengan Acute Lymphoblastic Leukaemia (ALL) disertai HIV Positif dan Hepatitis B Hartati, Hartati
Journal of Experimental and Clinical Pharmacy (JECP) Vol 3, No 2 (2023): August 2023
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v3i2.617

Abstract

Leukemia Limfoblastik Akut (ALL/LLA) adalah proliferasi ganas sel limfoid yang tersumbat pada tahap awal diferensiasi yang dapat menyerang sumsum tulang, darah, dan situs ekstrameduler. Penatalaksanaan leukemia meliputi terapi kuratif dan suportif. Studi kasus ini bertujuan untuk memperoleh gambaran tentang regimen kemoterapi pasien ALL yang disertai Hepatitis B Kronik dan HIV positif. Pengumpulan data menggunakan desain deskriptif dengan metode wawancara serta observasi langsung kondisi pasien dan e-rekam medik, e-resep yang dikaji kesesuaiannya berdasarkan protokol kemoterapi. Berdasarkan sequencing regimen kemoterapi diperoleh bahwa pasien menerima regimen Hyper CVAD dengan nilotinib, dimana penilaian resiko Chemotherapy Induced Nausea Vomiting diperoleh bahwa protokol kemoterapi yang diterima termasuk dalam kategori Moderate Emetogenic. Regimen ini juga disertai dengan pemberian Methotrexate dosis tinggi sebagai profilaksis CNS. Akan tetapi, untuk mengurangi resiko nefrotoksisitas akibat pemberian Methotrexate dosis tinggi, maka diperlukan tambahan natrium bikarbonat dan allopurinol untuk mencegah TLS pada pasien dengan ALL.Acute Lymphoblastic Leukaemia (ALL) is a malignant proliferation of lymphoid cells that are occlude at an early stage of differentiation and can invade the bone marrow, blood, and extramedullary sites. Management of leukemia includes curative and supportive therapy. This case study aims to obtain an overview of the chemotherapy regimen of ALL patients with chronic hepatitis B and HIV. Data were collected in a descriptive design with interview methods and direct observation of patient conditions and e-medical records. E-prescriptions were reviewed for suitability based on chemotherapy protocols. Based on the chemotherapy regimen sequencing, it was found that the patient received the hyper-CVAD regimen with nilotinib, while the chemotherapy-induced nausea and Vomiting risk assessment found that the chemotherapy protocol received was included in the Moderate Emetogenic category. This regimen is also accompanied by the administration of high doses of Methotrexate as CNS prophylaxis. However, to reduce the risk of nephrotoxicity due to the administration of high doses of Methotrexate, it is necessary to add sodium bicarbonate and allopurinol to prevent TLS in patients with ALL.
Formulasi Chewy Gummy “T-Sepis” (Temulawak, Serai, dan Jeruk Nipis) sebagai Upaya Peningkatan Kesehatan Masyarakat di Masa Pandemi Covid-19 Vania Armilda, Levina Hadi; Syamsiyah, Cici Nurul; Aji, Nur
Journal of Experimental and Clinical Pharmacy (JECP) Vol 3, No 2 (2023): August 2023
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v3i2.669

Abstract

Seluruh dunia terkena dampak puncak gelombang COVID-19. Kekebalan dapat ditingkatkan dengan senyawa yang disebut imunomodulator. Potensi temulawak, serai, dan jeruk nipis yang berkhasiat sebagai imunomodulator diformulasikan dalam bentuk sediaan chewy gummy. Metode yang digunakan yaitu eksperimental. Kombinasi zat berkhasiat menggunakan metode axial. Dilakukan uji organoleptik. Optimasi basis yang paling baik adalah formula 3 yaitu gelatin 40%, sukrosa 20%, dan akuadest 40%. Optimasi zat berkhasiat yang paling baik yaitu formula 4, konsentrasi paling tinggi terletak pada serai, sedangkan temulawak dan jeruk nipis mempunyai konsentrasi yang sama. Kesimpulan dari penelitian ini yaitu chewy gummy T-Sepis berpotensi sebagai imunostimulan.The whole world is being impacted by the peak of the COVID-19 wave. Immunity can be boosted by compounds called immunomodulators. The potency of temulawak, lemongrass, and lime, which are efficacious as immunomodulators, is formulated in the form of chewy gummy preparations. The method used is experimental. The combination of efficacious substances using the axial method. Organoleptic tests were carried out. The best base optimization is formula 3, namely 40% gelatin, 20% sucrose, and 40% aquadest. The best optimization of nutritious substances is formula 4. The highest concentration is found in lemongrass, while temulawak and lime have the same concentration. The conclusion of this study is that chewy gummy T-Sepis has the potential to be an immunostimulant.
Toxicity Activity and Total Phenolic Content of Soursop Leaves from Three Regions in South Sulawesi, Indonesia Rasyid, Faradiba Abdul; Amin, Asni; Sukmawati, Sukmawati; Djakariani, Kurnia Putri; Riska, Riska; Aliansyah, Moch. Rayhan; Sari, Cindy Artika
Journal of Experimental and Clinical Pharmacy (JECP) Vol 3, No 2 (2023): August 2023
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v3i2.674

Abstract

The fruit plant known as the soursop (Annona muricata L.) is indigenous to the Caribbean, Central America, and South America. The leaves of soursop contain substances with the potential to be cytotoxic, such as phenols and anonaceous acetogenins. Numerous variables, including geographic location, climatic conditions, and pest or disease disturbance factors, might impact a compound's phenol levels and toxicity. This study aims to investigate whether there are variations in the phenolic content and toxicity of the ethanol extract of soursop leaves that are grown in Gowa, Takalar, and Pinrang, three distinct regions in South Sulawesi, Indonesia. The Folin-Ciocalteu reagent was used to evaluate the total phenolic content, whereas Artemia salina Leach larvae were used for the BSLT method of the toxicity test. The results showed that the extracts of soursop leaves from Gowa, Pinrang, and Takalar had total phenolic contents of 3.7588% mg GAE/g, 0.9686% mg GAE/g, and 1.3832% mg GAE/g, respectively. Gowa, Pinrang, and Takalar have LC50 values of 38.19 µg/mL, 34.35 µg/mL, and 70.95 µg/mL, respectively, indicating their toxicity. The conclusion is that there were differences in the phenolic content and toxicity level of the ethanol extract of soursop leaves. All of these areas could source raw materials of soursop for the development of herbal medicines with cytotoxicity, i.e., anticancer, because they have LC50< 1,000 µg/mL.
Studi In Vitro Aktivitas Antibakteri Ekstrak Etanol Penawar Sampai (Tinospora crispa L.) terhadap Staphylococcus epidermidis Martani, Natalia Sri; Turnip, Oktaviani Naulita; Hanasia, Hanasia; Nawan, Nawan
Journal of Experimental and Clinical Pharmacy (JECP) Vol 4, No 2 (2024): August 2024
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v4i2.1074

Abstract

Kulit merupakan jaringan terluar di tubuh manusia yang menjadi pertahanan pertama dari masuknya patogen. Kulit dihuni oleh berbagai flora normal, salah satunya adalah Staphylococcus epidermidis. S. epidermidis adalah patogen oportunistik yang menjadi penyebab infeksi nosokomial yang menetap pada perangkat medis. Kasus infeksi nosokomial banyak terjadi baik di negara maju maupun negara berkembang. Infeksi nosokomial karena S. epidermidis tergolong sulit diatasi karena adanya kemampuan spesies ini dalam membentuk biofilm dan resistensi antibiotik yang menyebabkan kasus nosokomial berujung kematian. Penelitian ini mengujikan ekstrak dari tanaman penawar sampai atau brotowali terhadap S. epidermidis guna mendeteksi adanya aktivitas antibakteri. Batang penawar sampai dimaserasi dan diuapkan menjadi ekstrak kental dengan konsentrasi 20, 50, 75, dan 100%. Ekstrak kental batang penawar sampai diujikan terhadap S. epidermidis dengan metode difusi cakram. Interpretasi kemampuan ekstrak dalam menekan pertumbuhan S. epidermidis dapat diamati dari diameter zona hambat yang terbentuk di sekitar cakram. Konsentrasi terendah dalam menekan pertumbuhan S. epidermidis dihasilkan pada penelitian ini adalah konsentrasi ekstrak 20%. Hal ini mengindikasikan adanya potensi dari ekstrak penawar sampai sebagai antibakteri terhadap S. epidermidis yang tergolong dalam bakteri Gram positif.The skin is the outermost tissue in the human body and the first line of defense against pathogen entry. The skin is inhabited by a variety of normal flora, one of which is Staphylococcus epidermidis. S. epidermidis is an opportunistic pathogen that causes nosocomial infections that persist in medical devices. Cases of nosocomial infections are common in both developed and developing countries. Nosocomial infections due to S. epidermidis are relatively difficult to overcome due to the ability of this species to form biofilms and antibiotic resistance which causes nosocomial cases to lead to death. This study tested extracts from the antidote to brotowali plant against S. epidermidis to detect antibacterial activity. The stem was macerated and evaporated into thick extracts with concentrations of 20%, 50%, 75%, and 100%. The thick extracts were tested against S. epidermidis using disc diffusion method. Interpretation of the ability of the extract to suppress the growth of S. epidermidis can be observed from the diameter of the inhibition zone formed around the disc. The lowest concentration in suppressing the growth of S. epidermidis produced in this study was 20% extract concentration. This indicates the potential of the extract as an antibacterial against S. epidermidis which is classified as Gram-positive bacteria.
Formulasi dan Uji Aktivitas Antioksidan Sediaan Lip Balm dari Ekstrak Etanol Umbi Wortel (Daucus carota L.) dengan Metode DPPH (2,2-dipheny 1-picrylhydrazyl) Azima, Azima; Wahyuningsih, Sri; Agung, Yusfin Cesiana; Ilyas, Ika Lismayani
Journal of Experimental and Clinical Pharmacy (JECP) Vol 4, No 2 (2024): August 2024
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v4i2.1145

Abstract

Umbi wortel (Daucus carota L.) mengandung banyak senyawa kimia salah satunya yaitu β-karoten. β-karoten berfungsi sebagai antioksidan alami. Penelitian ini dilakukan untuk mengetahui aktivitas antioksidan sediaan lip balm dari ekstrak etanol umbi wortel dengan metode DPPH. Pada penelitian ini, kestabilan sediaan diketahui melalui evaluasi sebelum dan sesudah cycling test meliputi uji organoleptik, uji homogenitas, uji pH, uji daya sebar, uji titik lebur dan uji kelembapan yang sesuai standar kestabilan untuk sediaan lip balm serta metode dalam uji aktivitas antioksidan sediaan lip balm ekstrak umbi wortel menggunakan metode DPPH. Hasil penelitian menunjukkan bahwa sediaan lip balm ekstrak umbi wortel stabil secara fisika dan kimia setelah melalui tahapan evaluasi sediaan dan menunjukkan hasil pada IC50 yang diperoleh berturut-turut pada formula 1 yaitu 29,18 ppm, formula II yaitu 20,18 ppm, dan formula III yaitu 10,47 ppm sedangkan pada kontrol positif lip balm viva memiliki nilai IC50 yaitu 3,60 ppm. Kesimpulan dari penelitian ini yaitu, sediaan lip balm ekstrak umbi wortel dikategorikan stabil secara fisika dan kimia serta mempunyai nilai IC50 yang paling baik pada formula III (6%) dengan nilai IC50 yaitu 10,47 ppm.Carrot tubers (Daucus carota L.) contain many chemical compounds, one of which is β-carotene. β-carotene functions as a natural antioxidant. This research was conducted to determine the antioxidant activity of lip balm preparations from the ethanol extract of carrot tubers using the DPPH method. In this study, the stability of the preparation was determined through evaluation before and after the cycling test, including the organoleptic test, homogeneity test, pH test, spreadability test, melting point test, and humidity test in accordance with stability standards for lip balm preparations as well as methods for testing the antioxidant activity of lip preparations. carrot tuber extract balm using the DPPH method. The research results showed that the carrot tuber extract lip balm preparation was physically and chemically stable after going through the preparation evaluation stages and showed the IC50 results obtained respectively in formula 1, namely 29.18 ppm, formula II, namely 20.18 ppm, and formula III, namely 10.47 ppm, while the positive control Viva lip balm has an IC50 value of 3.60 ppm. The conclusion of this research is that the carrot tuber extract lip balm preparation is categorized as physically and chemically stable and has the best IC50 value in formula III (6%) with an IC50 value of 10.47 ppm.
Standarisasi Ekstrak Etanol Daun Sirsak (Annona muricata Linn.) Asal Daerah Gowa dan Takalar Amin, Asni; Rasyid, Faradiba Abdul; Syarif, Rezki Amriati; A.M, Sinta Faradilla; Saputri, Dicka; Sukmawati, Sukmawati
Journal of Experimental and Clinical Pharmacy (JECP) Vol 4, No 1 (2024): February 2024
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v4i1.972

Abstract

Tumbuhan sirsak (Annona muricata Linn.) memiliki aktivitas sebagai antikanker dan antiinflamasi. Bahan baku ekstrak daun sirsak yang akan dikembangkan menjadi obat modern Indonesia asli harus terstandardisasi untuk menjaga mutu ekstrak yang dapat dipengaruhi oleh tempat tumbuh. Penelitian ini bertujuan melakukan standarisasi ekstrak daun sirsak asal daerah Gowa dan Takalar. Masing-masing ekstrak daun sirsak dilakukan standarisasi dengan parameter spesifik dan non spesifik. Hasil penelitian diperoleh data untuk pengujian parameter spesifik berupa uji organoleptik ekstrak yaitu bentuk kental, warna coklat kehitaman, dan berbau khas. Kadar senyawa larut air dan etanol  sebesar 7, 560% dan 13,94%untuk Gowa, sedangkan Takalar sebesar 6,64% dan 10,1%. Hasil uji parameter non spesifik untuk daerah Gowa dan Takalar sebagai berikut, susut pengeringan 0,289% dan 0,293%, bobot jenis 0,814 g/mL dan 0,818 g/mL, kadar air 4,488% dan 7,436%, kadar abu total 4,467% dan 6,560%, kadar abu tidak larut asam 0,499% dan 0,248%, cemaran mikroba < 1,0 x 101 koloni/g, cemaran logam berat cadmium < 0,0001 µg/g,  cemaran logam timbal sebesar 0,0390 µg/g dan < 0,0471 μg/g, residu pestisida < 0,001 mg/kg. Penelitian ini menunjukkan ekstrak daun sirsak asal daerah Gowa dapat digunakan menjadi sumber bahan baku daun sirsak.Annona muricata Linn. has anticancer and anti-inflammatory activity. The raw material for soursop leaf extract, which will be developed into authentic modern Indonesian medicine, must be standardized to maintain the quality of the extract, which can be influenced by the place of growth. This research aims to standardize soursop leaf extract from the Gowa and Takalar areas. Each soursop leaf extract was standardized with specific and non-specific parameters. The results of the research obtained data for testing specific parameters in the form of an organoleptic test of the extract, namely thick form, blackish brown color, and distinctive smell. The levels of water-soluble compounds and ethanol were 7,560% and 13.94% for Gowa, while for Takalar they were 6.64% and 10.1%. The results of non-specific parameter tests for the Gowa and Takalar areas are as follows: drying loss 0.289% and 0.293%, specific gravity 0.814 g/mL and 0.818 g/mL, water content 4.488% and 7.436%, total ash content 4.467% and 6.560%, acid insoluble ash content 0.499% and 0.248%, microbial contamination < 1.0 x 101 colonies/g, heavy metal cadmium contamination < 0.0001 µg/g, lead metal contamination 0.0390 µg/g and < 0.0471 μg/g, pesticide residue < 0.001 mg/kg. This research shows that soursop leaf extract from the Gowa area can be used as a source of raw material for soursop leaves.
Identifikasi Senyawa Flavonoid Fraksi Etanol Bintang Laut (Linckia laevigata) di Perairan Bolaang Mongondow Mappa, Moh. Rivaldi; Bahi, Rizky Resvita R.; Sarman, Sarman; Fauzan, Moh Rizki; Begum, Nanda Sayyida
Journal of Experimental and Clinical Pharmacy (JECP) Vol 4, No 2 (2024): August 2024
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v4i2.1103

Abstract

Bintang laut merupakan organisme laut yang menghasilkan senyawa bioaktif, salah satunya flavonoid. Flavonoid berkhasiat sebagai antioksidan, anti-penuaan, anti-inflamasi, antivirus, dan lain sebagainya. Penelitian ini bertujuan untuk mengidentifikasi kandungan senyawa flavonoid dari fraksi etanol bintang laut yang diperoleh dari pesisir pantai Desa Maelang Kabupaten Bolaang Mongondow menggunakan uji kualitatif pereaksi kimia dan metode kromatografi lapis tipis (KLT). Hasil penelitian menunjukkan bahwa bintang laut yang diperoleh dari pesisir pantai Desa Maelang Kabupaten Bolaang Mongondow positif mengandung senyawa flavonoid yang ditandai dengan perubahan warna dari jingga ke hijau kehitaman pada uji menggunakan pereaksi kimia dan terbentuknya pola noda berwarna hijau dengan nilai Rf 0,8 pada pelat KLT yang dibandingkan dengan baku standar kuersetinStarfish are marine organisms that produce bioactive compounds, one of which is flavonoids. Flavonoids are efficacious as antioxidants, antiaging, anti-inflammatory, antiviral and so on. This research aims to identify the content of flavonoid compounds from the ethanol fraction of starfish obtained from the coast of Maelang Village, Bolaang Mongondow Regency using qualitative tests of chemical reagents and the thin layer chromatography (TLC) method. The results of the research showed that starfish obtained from the coast of Maelang Village, Bolaang Mongondow Regency were positive for containing flavonoid compounds which were characterized by a color change from orange to blackish green in the test using chemical reagents and the formation of a green stain pattern with an Rf value of 0.8 on the TLC plate which was compared with the standard quercetin
Potensi Ekstrak Etanol Daun Murbei (Morus alba L.) sebagai Antifungi terhadap Jamur Candida albicans Utami, Yuri Pratiwi; Rante, Herlina; Handayani, Tuti; Jariah, Ainun; Erianto, Fatricia
Journal of Experimental and Clinical Pharmacy (JECP) Vol 4, No 1 (2024): February 2024
Publisher : Poltekkes Kemenkes Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52365/jecp.v4i1.969

Abstract

Senyawa kimia di daun murbei (Morus alba L.), termasuk flavonoid, tanin, steroid, dan saponin. Salah satu golongan senyawa yaitu flavonoid memiliki sifat antioksidan, antibakteri, dan sifat antimikroba yang dapat meningkatkan kontraksi luka. Penelitian dilakukan untuk mengetahui bagaimana potensi daun murbei sebagai antifungi terhadap jamur Candida albicans. Untuk menghasilkan ekstrak yang akan diskrining fitokimia, teknik maserasi menggunakan pelarut etanol 70% digunakan untuk ekstraksi sampel. Uji bebas etanol dan dilanjutkan dengan pengujian antifungi terhadap jamur Candida albicans. Hasil ekstraksi memperoleh persen rendemen ekstrak 3,89%. Pengujian skrining fitokimia mengandung flavonoid, saponin dan tannin. Pengujian ekstrak dengan konsentrasi 1,25%, 2,5%, 5% dan 10% memiliki aktivitas sebagai antifungi terhadap Candida albicans yaitu 9,7 mm ± 0,36, 10,83 mm ± 0,45, 15,8 mm ± 3,31, 26,06 mm ± 3,27 dan ketokonazole sebagai kontrol positif 33,4 mm ± 0,57. Disimpulkan bahwa ekstrak etanol daun murbei memiliki aktivitas sebagai antifungi terhadap Candida albicans. Ekstrak etanol daun murbei dapat menghambat pertumbuhan Candida albicans hingga konsentrasi 1,25%, sehingga dapat dimanfaatkan sebagai antijamur.Chemical compounds in mulberry leaves (Morus alba L.) include flavonoids, tannins, steroids, and saponins. One class of compounds, namely flavonoids, has antioxidant, antibacterial, and antimicrobial properties that can increase wound contraction. Research was conducted to determine the potential of mulberry leaves as an antifungal against the fungus Candida albicans. To produce extracts that will be screened for phytochemicals, a maceration technique using 70% ethanol solvent is used for sample extraction. Ethanol-free test and continued with antifungal testing against Candida albicans fungus. The extraction results obtained an extract yield percentage of 3.89%. Phytochemical screening tests contain flavonoids, saponins, and tannins. Extract testing with concentrations of 1.25%, 2.5%, 5%, and 10% had antifungal activity against Candida albicans, namely 9.7 mm ± 0.36, 10.83 mm ± 0.45, 15.8 mm ± 3.31, 26.06 mm ± 3.27, and ketoconazole as a positive control (33.4 mm ± 0.57). It was concluded that the ethanol extract of mulberry leaves has antifungal activity against Candida albicans. Mulberry leaf ethanol extract can inhibit the growth of Candida albicans up to a concentration of 1.25%, so it can be used as an antifungal.

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