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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

Arjuna Subject : -

Articles 824 Documents

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Respon Imun Mencit terhadap Protein 24 dan 71 kDa Toxocara vitulorum dalam Membentuk Antibodi dan Protektifitasnya terhadap Infeksi Buatan Candra Dwi Atma; Kusnoto Kusnoto; Eduardus Bimo Aksono HP.
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This study aimed to get 24 and 71 kDa protein of T. vitulorum that have a high antigenicity and imunogenicity on ELISA and to get the protein which able to protect mice against artificial infection of L2 T. vitulorum. This study using mice Balb/c aged 6 to 8 weeks. Proteins isolated were 24 and 71 kDa. Proteins 24, 71 kDa and intestinal homogenates immunized in mice with the addition of adjuvant (1: 1) for 3 times with period of 2 weeks. Two weeks after the last booster, serum drawn from mice tested by Indirect ELISA to determine the value of optical density (OD). The next stage, mice were infected L2 with a dose of 10-17 larvae / g of body weight. The results showed the average OD value by ANOVA Factorial antigen P24 was not significantly different with antigen P71 T. vitulorum. Antigen 24 kDa and 71 kDa with different immunization, both were showed P0 significantly different with P1, P2 and P3. Based on percentage of L2 in the somatic tissue of mice, P0 were showed 79.1% of total number of L2 early infection, whereas the treatment of P1 were showed 0.04%, P2 and P3 showed as much as 0.02% and 0.04%. 24 and 71 kDa protein of T. vitulorum that have a high antigenicity and imunogenicity.

Efek Ekstrak Air Biji Pepaya (Carica papaya L.) terhadap Fertilitas Mencit (Mus musculus L.) Betina Muhammad Feraldi Firdaus; Agung Janika Sitasiwi; Siti Muflichatun Mardiati
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Papaya seeds were known as medicinal plant which have anti-fertility potencies. So it could be used as a herbal contraceptive. The purpose of this study was to test the anti-fertility effect of Papaya seed water extract on mice (Mus musculus L.) fertility which proved by pregnancy rate, litters number and sex ratio of litters. This study was designed by Completely Randomized Design, using 15 female Swiss Webster mice that were divided into 5 treatment groups with triplicates. The K(-) group were treated by distilled water; K(+) group were treated by contraceptive pills; P1, P2, P3 groups that were treated by papaya seeds extract with 1.4, 3.5 and 7 mg/animal/day respectively. Each treatment was given orally with 0.5 ml in volume for 21 days. At the end of treatment, mice was mated. The observed parameters were pregnancies occurence, litters number and sex ratio of the litters. The litters number data were analyzed by Analysis of Variance. The results showed there was differences in pregnancy time and sex ratio between control and treatment groups, but there was not significantly different in litters number. It could be concluded that the fertility of mice was inihibited by water extract of papaya seeds.

Efektivitas Terapi Multivitamin, Obat Cacing dan Premiks pada Sapi Terdiagnosa Hipofungsi Ovarium di Wilayah Kecamatan Prambanan, Yogyakarta Niken Widarini; Imbang Ru Beda; Agustina Dwi Wijayanti
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Ovarian hypofunction in cattle is reproduction disorder related to massive economic loss. Etiology of this ovarium disfunction are low quality feed, minimum health concern, poor sanitation and pen problem. Eighty-five (85) Peranakan Ongole (PO), Peranakan Limosin (PL) and Peranakan Simental (PS) cows from Prambanan Sub District of Daerah Istimewa Yogyakarta were diagnosed with ovarian hypofunction by rectal palpation and physical examination. The cows were then programmed for ovary condition recovery by administration of vitamine A,D,E (IM), per oral anthelminthic bolus (Klosantel) and premix mixture in feed given daily for 4 weeks. After 4 weeks, the cows were re-examined. Sixty-two (72,9%) cows were recovered from ovary hypofunction, however ovaries of 23 (21,9%) cows were palpated abnormal, which require another administration of A,D,E (IM). There were no significant difference between the type of cows (PO,PL,PS) with recovery percentages after one programme therapy (P>0.05). It can be concluded that single injection of vitamin A,D, E , per oral bolus of Klosantel, and addition of premix into feed daily for 4 weeks were able to reduce 72,9% ovary hypofunction in Peranakan Ongole, Peranakan Limosin and Peranakan Simental cows at Prambanan sub district, Daerah Istimewa Yogyakarta.

Karakteristik Fisik dan Kimia Telur Burung Mamoa (Eulipoa Wallacei) di Pantai Uwo Uwo Kecamatan Galela Kabupaten Halmahera Utara Yusri Sapsuha; Nur Sjafani; Nurjana Albaar; Hasriani Ishak
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The aim of this research was to determine physicochemical characteristics of Mamoa egg. The eggs were collected about 100 eggs from Uwo uwo beach. Physical quality was observe in Animal Husbandry laboratory, Faculty of Agricultural, Khairun University. The physical characteristics observed were measuring include weight, shape, colour of eggshell, length, diameter, egg index, albumen, yolk, weight of eggshell, haugh unit, and thickness of eggshell. The chemical characteristics consist of moisture content, protein, fat content, fiber content, ash, carbohydrate, cholesterol, and antioxidant. Data were analyzed by quantitative descriptive analysis. The result showed that physical quality of egg were weight = 97.28 g, length = 7.12 cm, diameter = 5.45 cm, index of egg = 71.89%, Percentage of yolk and albumen were 69.12% and 23.24%, haugh unit score = 68.78 and shape of egg was ovale. The chemical characteristic of egg chemical quality were protein content = 15.47%, fat content = 8.31%, carbohydrate = 25.52 %, cholesterol = 387.54 mg/100gr, and antioxidant = 49.08% dpph.

Daya Vermisidal Ekstrak Lima Jenis Etnofarmakologi terhadap Cacing Haemonchus contortus Secara In-vitro I Gusti Komang Oka Wirawan; Kurniasih Kurniasih; Joko Prastowo; Wisnu Nurcahyo
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Research aimed to identify the most effective concentration of five extract types (EKBMCP, EKPAS, EDMSP, EBJCP, dan EDMCO) as vermicidal anthelmintic against H. contortus in-vitro. Research was consisted of seven groups, among them five groups were considered as treatment groups that named based on the extract type (EKBMCP, EKPAS, EDMSP, EBJCP, dan EDMCO). Each treatment group was consisted five subgroups of etnopharmacological concentration : 0,5%, 1,5%, 2,5%, 3,5% dari 0,2 g/mL. Other two group were regarded as a negative control by distilled water treatment, and a positive control by pyrantel pamoat 5%. Every single treatment group was quadruplet and exposure each concentration were performed in quadruplet with variation in exposure times i.e. : 1, 3, 5, and 7 hours. The research focused on several variables that were the percentage of live and dead H. contortus in the treatment groups, extract concentration, and treatment period by using selected etnopharmacological extract. The effective vermicidal anthelmentic concentration of each treatment was analyzed descriptively. The results showed that five etnopharmacological extract types that were EKBMCP, EKPAS, EDMSP, EBJCP, dan EDMCO had a potency as vermicidal anthelmentic. Among those treatment, EKPAS and EBJCP had the lowest concentration (2,5%) to produce optimum vermicidal effect (100%) with period five hours exposure. Other treatment groups with EKBMCP and EDMSP needed 3,5% concentration with exposure period seven hours to produce optimum vermicidal effect, respectively. Another treatment group with EDMCO needed 3,5% concentration with exposure time seven hours to produce optimum vermicidal effect.

Kualitas Oosit Kerbau dari Status Reproduksi Ovarium yang Berlainan Sri Gustina; Hasbi Hasbi; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

In vitro embryo production relies on the quality of oocytes, however the quality is subjected to ovaries reproduction cycle. This study was conducted to observe the potency of buffalo ovaries from various reproductive cycle in producing quality oocytes. Collected pairs of ovaries from slaughter house were weighed and grouped of 5 according to the cycle. Oocytes were collected by slicing techniques, then selected based on quality. The results showed the average weight of buffalo ovaries of (+CL, +FD); (+CL, -FD); (-CL, +FD); (-CL, -FD) are 7.2 g; 5.5 g; 4.1 g; and 4.5 g respectively. No significant quality difference of produced oocytes between ovaries cycles (P>0.5). Good quality of collected oocytes were only 40-55%. Approximately 2-5 oocytes of grade A and 1-5 oocytes of grade B can be collected per pair of ovaries.

Pengaruh Ekstrak Buah Delima Terstandar 40% Ellagic Acid terhadap Profil Darah Tikus Putih Yang Mengalami Nefrotoksisitas akibat Induksi Gentamisin Bambang Sektiari Lukiswanto; Wiwik Misaco Yuniarti
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The purpose of this study was to determine the effect of pomegranate extract of 40% ellagic acid on blood profile of albino rats that had nephrotoxicity after included with Gentamicin. a total of 32 white rats were divided into four treatment groups, each consisting of eight rats. the four treatment groups were healthy rats (P0), Gentamicin induced rats (P1), Gentamicin induced rats and ellagic acid treatment (P2) and Gentamicin induced rats and standardized Pomegranate extract therapy 40% ellagic acid treatment (P3). After seven days of treatment, blood was taken for blood profile tests. The results showed that in the Gentamicin-induced group (P0), a decrease in blood profile allegedly caused by Gentamicin that could cause damage and impaired renal tubular function. Givibg ellagic acid and pomegranate extracts of 40% ellagic acid proved to improve the blood profile of Gentamicin induced rats. This is due to inhibition of renal damage and improved renal function due to antioxidant activity possessed by ellagic acid and pomegranate extract of standardized 40% ellagic acid.

Studi Distribusi Glukosa Transporter 4 pada Otot Skelet Ayam Kedu Cemani Teguh Budipitojo; Ariana -; Tri Wahyu Pangestiningsih; Hery Wijayanto; Dwi Liliek Kusindarta; Dewi Kania Musana
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Glucose transporter (GLUT 4) is glucose transporter protein regulated by insulin, found in adipose tissue and striated muscle (skeletal and cardiac muscle). Kedu cemani chicken is one of Indonesia endemic animal, found in Kedu, Temanggung regency, Central Java. This study was required to complete microscopic documentation of Indonesia’s native biodiversity. The objective of this study was to clarify GLUT 4 distribution in skeletal muscle fibers of kedu cemani chicken by using avidin-biotin-peroxidase complex (ABC) immunohistochemistry method. This study was conducted by using pectorales major, biceps brachii, and biceps femoris muscle tissue from 5 kedu cemani chicken. The result showed that GLUT 4 immunoreactivity were detected in sarcolemma and myofibrils component of pectorales major, biceps brachii, and biceps femoris muscle tissue. Intensity of GLUT 4 immunorectivites increased from weak intensity in pectorales major muscle tissue, moderate intensity in biceps brachii muscle tissue, then strong intensity in biceps femoris muscle tissue. This result might motivate to further exploration about the other kedu cemani chicken specific features to complete microscopic documentation of Indonesia’s native biodiversity.

Potensi Ekstrak Atuna racemosa sebagai Anti - Methicillin Resistant Staphylococcus aureus (MRSA) Siti Isrina Oktavia Salasia; Novra Arya Sandi; Fajar Budi Lestari; Verda Farida; Nurbani Aziz
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Staphylococcus aureus is one of the major causative agents of mastitis in animals and a variety of human diseases such as septicemia, endocarditis, arthritis dan osteomyelitis. Infection of Methicillin-resistant S. aureus (MRSA) has been widely reported and these strains are usually resistant to multiple antibiotics. The purpose of this study was to evaluate the potential of Atuna racemosa, as an alternative herbal medicine against MRSA infection. The MRSA strains were isolated from human and confirmed based on their resistant to various antibiotics and analyzing of the mecA gene by polymerase chain reaction (PCR). Atuna racemosa originated from Ambon, Maluku, Indonesia, were extracted using 70% ethanol. The activities of the Atuna racemosa extract against MRSA were performed by diffusion disc agar and dilussion agar tests. The results showed that Atuna racemosa extract has the barrier effect of MRSA growth at a concentration of 5% in the diffusion test and at a concentration of 7% in the dilution test. Atuna racemosa could be used as an alternative new drugs with dose of 0.07 g/ml (7%) against MRSA which is multi-resistant to many antibiotics.

Kondisi Biosekuriti Tempat Penjualan Burung Terkait Avian Influenza di Wilayah Jakarta Ardilasunu Wicaksono; Etih Sudarnika; Chaerul Basri
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The aims of this study were to identify biosecurity condition and to analyse the relation of bird vendors characteristics toward biosecurity practices and biosecurity condition related to Avian influenza in Jakarta bird markets. Data were collected from four bird markets in Jakarta using randomly interview method of 75 respondents and observe the biosecurity practices in bird markets. Data were analysed using chi-square test and gamma test to analyse the relation between bird vendors’ characteristics and biosecurity condition. The result showed that 69,3% of bird vendors did the biosecurity practice in moderate level and only 10,7% did it well, meanwhile, most of bird kiosks (53,5%) in Jakarta got the poor category of biosecurity level. Beside that, bird vendors’ characteristics which has the significant relation (p<0,05) were age (p=0,003), education level (p=0,007), training (p=0,047), knowledge (p=0,000) and attitude (p=0,000) toward biosecurity condition related to Avian influenza in Jakarta bird markets.

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