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Jurnal Sain Veteriner
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Arjuna Subject : -
Articles 824 Documents
Identifikasi Ektoparasit pada Benih Ikan Mas (Cyprinus carpio) di Balai Benih Ikan Kabat, Kabupaten Banyuwangi Mohammad Faizal Ulkhaq; Darmawan Setia Budi; Gunanti Mahasri; Kismiyati -
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (12210.612 KB) | DOI: 10.22146/jsv.34702

Abstract

Diseases caused by parasitic groups get less serious attention from the farmers because it is chronic morbidity and mortality rates and small. However, if observed further, parasitic diseases may trigger secondary infection by bacteria, viruses and fungi that can cause death and high losses. Thus this study aims to identify the types of ectoparasites that infected the seed goldfish in BBI Kabat, Banyuwangi which the results will be used as an early warning to prevent the death of fish in bulk. The research sample in the form of seeds 3-5 cm carp amounted 150 obtained from BBI Kabat, Banyuwangi. Ectoparasites inspection method performed by scraping the surface of the body, fins and gills of fish, and then observed under a microscope with a magnification ranging from 40-400x. The observation of ectoparasites are further identified accordance morphology based on the keys of identification. Identified ectoparasites infect seed goldfish in BBI Kabat, Banyuwangi consists of 8 genus is Zoothamnium sp., Trichodina sp., Oodinium sp., Vorticella sp., Argulus sp., Lernaea sp., Dactylogyrus sp., and Gyrodactylus sp. Further research is needed to determine the prevalence and intensity of parasites and appropriate measures to prevent the spread of parasites in healthy fish in BBI Kabat, Banyuwangi. 
Efektifitas Penambahan Insulin dalam Media Maturasi dan atau Media Kultur pada Tingkat Maturasi Oosit dan Perkembangan Awal Embrio Sapi secara In Vitro Ni Wayan Kurniani Karja; Syafri Nanda; Mohamad Agus Setiadi
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1994.908 KB) | DOI: 10.22146/jsv.35565

Abstract

This study was aimed to determine the effect of insulin supplementation in maturation and/or culture medium on nuclear maturation rate and the early bovine embryo development in vitro. Oocytes were collected and matured in maturation media without (IVM I-) or with (IVM I+) 10 ug/µL insulin at incubator 5% CO2 and the temperature of 39 °C, for early embryonic development evaluation, oocytes were divided into 4 groups, without the supplementation of insulin to the maturation medium and culture (IVM I-/IVC I-), insulin supplementation only the maturation medium (IVM I+/IVC I-), insulin supplementation only in the culture medium (IVM I-/IVC I+), and the combination of insulin supplementation to the maturation medium and culture (IVM I+/IVC I+). The result showed that supplementation of insulin to the maturation medium increased number nuclear maturation was higher 87.7% (P<0.05) compared to treatment without supplementation of insulin (70.1%). Cleavage rate in treatment IVM was higher IVM I-/IVC I- (55.8%), IVM I+/IVC I- (64.1%), IVM I-/IVC I+ (59.9%) (P<0.05). Result of the other were showed that early bovine embryo on day-4 cultured (IVC) reached 16 cell on treatment IVM I-/IVC I+(31,9%) and IVM I+/IVC I+ (27,1%) were higher compared to treatment IVM I-/IVC I-(2.9%) and IVM I+/IVC I- (2.5%) (P<0.05). In conclusion, supplementation of insulin to maturation medium and culture medium can increase nuclear maturation rate and improved early embryo cleavage rate.
Hiperglikemia Tidak Menyebabkan Perubahan Bobot Badan dan Jumlah Neuron Mienterik Nitrergik Usus Halus Tikus Wistar (Rattus norvegicus) Amelia Hana; Pudji Astuti; Yuda Heru Fibrianto; Sarmin Sarmin; Claude Mona Airin; Tauhid Tauhid
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (11051.986 KB) | DOI: 10.22146/jsv.38409

Abstract

Digestive dysfunction is a common symptom of diabetes. Diabetes can lead to the loss of enteric neurons as well as nerve dysfunction and gastrointestinal complications. There has been no complete report of mienteric nitrergic of small intestines on hyperglycaemia condition, so the purpose of this study was to assess changes of nitrergic neural profile of small intestines on hyperglycemic Wistar strain rats (Rattus norvegicus). Twelve rats were adapted for 3 days, then they were randomly divided into 3 groups namely group I (K-1, as control), group II (K-2), and group III (K-3). Before treatment all rats were fasted for 12 hours, rats K-1 were induced with 1.0 ml of buffer citrate single peritoneal dose; rats K-2 were induced with 40 mg streptozotocin (STZ)/ kg body weight of single peritoneal dose; and rats K-3 were induced with 45 mg STZ /kg body weight of single peritoneal dose. On the 1st and 5th days after STZ induction, blood was collected through the coccigea vein to measure blood glucose levels and weigh the weight. On the day 5th the rats were uthanated with a high dose of ketamine, dissected and taken small intestines (duodenum, jejunum, and ileum) for staining with Nicotinamide Adenine Dinucleotide Phosphate-diaphorase (NADPH-d). Morphometric changes of nitrergic neurons of the small intestine segments were observed. The results showed that on the 5th day increased blood glucose levels of the K-2 and K-3 groups were significantly higher than K-1 (P <0.01). The weight of three groups did not show any significant improvement difference (P> 0.05). The number of neurons in the duodenum, jejunum and ileum of K-2 group increased higher than that of K-1 and K-3 groups. These results indicate that in hyperglycaemic states, the number of nitrergic neurons increases (K-2 group), and there is a significant decrease in the number of neurons (group K-3) compared with K-1 and K-2 groups (P <0.01). The results of this study concluded, that the STZ induction treatment causes hyperglycemia. Hyperglycemia does not affect to the body weight and the number of  nitrergic neurons of small intestines in Wistar strain rats (Rattus norvegicus).
Daya Ovisidal dan Larvisidal Infusa Daun Bambu Apus (Gigantochloa apus)Terhadap Haemonchus Contortus Secara in Vitro Budi Purwo Widiarso; Wisnu Nurcahyo; Kurniasih Kurniasih; Joko Prastowo
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7949.865 KB) | DOI: 10.22146/jsv.38430

Abstract

Gastrointestinal Nematodes are one among cause decrease production efificiency in goat and sheep in Indonesia, especially parasite of abomasum namely Haemonchus contortus. This parasite causes disease of Haemonchosis causing anemia, and causes mortality to young goat or sheep. Loss of economics caused by high morbidity and  mortality this parasite so that can improve the expense of prevention and medication of this disease. This research aim was to evaluate ovicidal and larvicidal apus bamboo leaf infusion (Gigantochloa apus) against Haemonchus contortus by in vitro. Examination of apus bamboo leaves infusion to seven concentrations namely : 1.2; 1.0; 0.8,; 0.6; 0.4; 0.2;0.1; 0.05 and 0 mg / ml as negative control. Positive control uses Albendazole with concentration 0,025 mg / ml. The highest concentration 1.2 mg / ml apus bamboo leaves infusion   inhibited 92.35% egg hatching ( LC 0.49 mg / ml ) and have larvicidal activity equal to 90% ( LC50 0,47mg / ml). The concentration 1.2 mg / ml also have similar effect with positive control which use Albendazole. Phytochemical apus  bamboo leaf analysis of the highest concentration revealed the presence  tanin condensed, saponins, and alkaloids.
Deteksi Hemaglutinin, Hemolisin dan Koagulase Secara Fenotipik dan Genotipik pada Staphylococcus aureus Isolat Asal Broiler Khusnan Khusnan; Dwi Kusmanto
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (12538.886 KB) | DOI: 10.22146/jsv.38434

Abstract

Staphylococcus aureus is a bacterium causing diseases in animals and human. Staphylococcus aureus in broilers cause septicemia, tendosinovitis, dermatitis, endocarditis, wound infections, arthritis and bumblefoot. In this research, 24 isolates of Staphylococcus aureus from broiler were characterized of its virulent factors including the presence of haemagglutinin, ability to agulate the plasma in tubular coagulase test as well presipitate formation in clumping factor test, and haemolysis types. By polymerase chain reaction (PCR) were genotypically detected genes coa, clf, hlaA, and hlaB. All of the isolates (100%) had haemagglutinin, capable to agglutinate and precipitae of rabbit plasma. All isolates could lyse sheep red blood cells with the type of α-hemolysis (45.8%),  β-hemolysis (50.0%) and γ-haemolysis (4.2%). Genotypically, all isolates (100%) had coa and clf genes,  hlaA gene 7(0.8%) and hlaB gene (29.2%).
Identifikasi Escherichia coli dan Salmonella spp pada Karkas Sapi di Rumah Potong Hewan di Banyuwangi dan Resistensi Terhadap Antibiotika Faisal Fikri; Muhammad Thohawi Elziyad Purnama; Amung Logam Saputro; Iwan Sahrial Hamid
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8447.455 KB) | DOI: 10.22146/jsv.38439

Abstract

Food borne disease can be transmitted through Escherichia coli and Salmonella spp contamination. The contamination of microorganisms with high pathogenic potentials on bovine carcasses results in food borne illness. The aim of this study was to identify multidrug-resistance of Escherichia coli and Salmonella spp on carcass samples that isolated from slaughter house in Banyuwangi. Samples were collected from district of Banyuwangi, Rogojampi, Genteng and Kalibaru. This study used cross sectional study with assumption of prevalence at 50% in each contaminant, confidence level 95% and standart of error at 10%. By the number of samples should reach 96 samples. The result showed that seven samples (7,3%) were positive Escherichia coli and none samples (0%) were positive Salmonella spp. The multidrug-resistance of Escherichia coli showed that Cephalotin (42,9%), Trimethoprim (14,3%) and Erythromycin (42,9%) whereas Ampicillin, Enrofloxacin, Chloramphenicol and Tetracycline were sensitive against Escherichia coli.
Perbandingan Kinerja Berahi dan Level Estradiol Kambing Kacang dan Kambing Nubian yang Diinduksi dengan PGF2α Mulyadi Adam; Raja Nurul Huda; Widya Zahara; Tongku Niswan Siregar; Sri Wahyuni; Cut Nila Thasmi; Rosmaidar Rosmaidar
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (10432.789 KB) | DOI: 10.22146/jsv.38444

Abstract

The experiment was conducted to compare estrous performance between and estradiol level kacang and Nubian goats estrous induction by prostaglandin F2 alpha (PGF2α). Eight female goats were divided into two groups, groups 1 (K1) consisted five Nubian goats and group 2 (K2) included three kacang goats with criterias as follows: age 1.5-3.0 years old, not pregnant, at least two months postpartum, and healthy clinically. All goats were estrous synchronized by double injection 7,5 mg PGF2α intramuscularly with 10 days interval. Goats that appeared estrus symptoms were mated naturally to a male goat. Determination of estrous intensity was performed using scoring method. Blood samples were taken at the first time the goats were mated (day 0). The measurement of estradiol concentration was done by enzyme linked immunosorbent assay (ELISA) method. Data of intensity, onset, duration, and estradiol level were analyzed using t test. The results showed that the rate of intensity, onset, duration, and estradiol level of estrous at KI vs KII were 2.08±0.52 vs 1.85±0.67, 56.00±34.11 vs 40.80±18.20 hours, and 24.00±26.15 vs 33.80±9.10 hours, and 103.51±42.49 vs 67.95±38.44 pg/ml (P>0,05). It can be concluded there was no difference of estrous performance and estradiol level between kacang and Nubian which estrous induction with PGF2α. The relationship between the intensity of estrus estradiol level in the Nubian goats was 0.995 while the kacang goats was 0.890.
Analisis Scanning Electron Microscope (SEM) Mikroarsitektur Daerah Metafisis Os Femur Tikus Putih (Rattus novergicus) Ovariohisterektomi dengan Pemberian Madu Lebah (Apis dorsata) Hutan Sumbawa Samsi Yordan; Abdullah Hasib; Muhammad Huda Ramadhan Ibrahim; Siti Nur Rohmah; Salsabilla Abani; Ira Sari Yudaniayanti
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9095.229 KB) | DOI: 10.22146/jsv.38446

Abstract

The aims of this study to determine the effect of honey bees (Apis dorsata) to prevent the decrease of femur density in  ovariohysterctomized rats as animal model of osteoporosis by Scanning Electron Microscope (SEM). This study used 20 adult female white rats (Rattus norvegicus) with body weight 300 gr. Divided into 5 groups, negative control group (P0-), not in ovariohysterectomy,  only given aquadest 1.5 ml/day ; Positive control group (P0 +), in ovariohysterectomy,  only given aquadest 1.5 ml/day; Treatment group 1 (P1), in ovariohysterectomy and given honey 1 gram / kg BB + 1.5 ml aquadest; Treatment group 2 (P2), in ovariohysterectomy and given honey 2 grams / kg BB + 1.5 ml aquadest; Treatment group 3 (P3), in ovariohysterectomy and given honey 4 grams / kg BB + 1.5 ml aquadest. After 12 weeks, all rats were sacrified for removal of the femur and performed by SEM. Analysis of SEM showed that mice administered honey with a dose of 4 grams / kg BW (P3) showed bone density approaching negative control treatment (P0-), ie, ovariohysterectomized rats. P3 showed the most less bone porosity than other treatment groups. The largest and most uniformly distributed porosity was shown by the positive control group (P0 +). The best results with the smallest quantity and size of the porosity of femur is in P3 group. In conclusion, honey (Apis dorsata) can prevent the decrease in bone density of the femur by Scanning Electron Microscope (SEM).
Gambaran Darah Kukang Dipelihara pada Kandang Penangkaran Wirdateti Wirdateti; Ni Luh Putu Rischa Padmacanthy; Raden Taufiq Purna Nugraha; Gono Semiadi
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8977.206 KB) | DOI: 10.22146/jsv.38448

Abstract

Maintenance of wildlife outside its original habitat is usually for the purposes of the exhibition in zoos, for research and captive breeding effort, especially for endangered species which eventually return to nature. The adaptability of the animals outside their original habitat can be seen from their health and their ability to reproduce in ex situ (captive) environment. One of the parameter that can be used to determine a health an animal is by observing their blood parameters and compared it with normal reference, however for many wild animals reference data for normal blood parameters are limited or not available. This study was conducted to determine a normal reference of blood parameters of slow lories that are kept in captive breeding condition, consisted of routine hematology and blood chemistry. This study used five animals consisting of four of N. coucang (1 male and three female) and one female N. javanicus. The results of the analysis showed that the value of hemoglobin, MCV, PDW, MPC, and erythrocytes (RBC) were higher on the male slow lories, while leukocytes (WBC), hematocrit, and MCHC were higher on the female. There were no significant differences between blood parameters of N. coucang and N. javanicus.
Pengaruh Serbuk Jambe (Areca catechu) Dibandingkan Serbuk Gabungan Jambe (Areca catechu) dan Binahong (Anredera cordifolia) Terhadap Ascariasis pada Ayam Buras Okti Herawati; Kurniasih Kurniasih; Joko Prastowo
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8078.461 KB) | DOI: 10.22146/jsv.38449

Abstract

Infestasi Ascaridia galli mengakibatkan penurunan produktivitas dari ayam buras baik daging maupun telur. Pengendalian infestasi A. galli dilakukan menggunakan tanaman yang mengandung antiparasit sebagai anthelmintik. Penelitian ini bertujuan untuk mengetahui efek pemberian serbuk Jambe dan serbuk gabungan Jambe dengan Binahong terhadap infestasi Ascaridia galli pada ayam buras berdasarkan jumlah egg per gram (epg) feses, berat badan, jumlah cacing A.galli, dan perubahan histopatologi usus. Hewan yang digunakan dalam penelitian ini ialah ayam buras yang berumur 2 bulan dan positif terinfestasi cacing Ascaridia galli. Dilakukan penghitungan jumlah egg per gram (epg) dan berat badan sebelum perlakuan. Sebanyak 10 ekor ayam buras yang terbagi ke dalam 2 kelompok. Kelompok 1 diberi serbuk Jambe dengan dosis 0,1mg/kg BB sedangkan kelompok 2 diberi serbuk gabungan Jambe 0,05mg/kg BB dan Binahong 0,0125mg/kg BB. Pengobatan dilakukan secara per oral selama 7 hari, pada hari ke 10 dilakukan eutanasi. Data hasil perhitungan jumlah egg per gram (epg), jumlah cacing, berat badan dan perubahan histopatologi dianalisa secara diskriptif. Pemberian serbuk Jambe maupun serbuk gabungan Jambe dan Binahong dapat menurunkan jumlah epg, meningkatkan berat badan ayam dan membunuh cacing A.galli sebesar 80% untuk kelompok 1 dan 40% untuk kelompok 2. Perubahan histopatologis usus halus terlihat adanya infiltrasi sel radang berupa eosinofil dan limfosit di duodenum, jejunum dan ileum serta terdapat potongan cacing cestoda pada kedua kelompok. Serbuk Jambe lebih berpotensi sebagai anthelmentik dibandingkan serbuk gabungan Jambe dan Binahong dilihat dari penurunan jumlah epg, peningkatan berat badan dan jumlah cacing pada usus. Pemberian serbuk Jambe maupun serbuk gabungan Jambe dan Binahong dapat membunuh nematoda tetapi tidak untuk cestoda.

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