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Jurnal Kedokteran Hewan

JKH
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Published by Universitas Syiah Kuala

ISSN : 1978225X EISSN : 25025600 DOI : 10.21157

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.

Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)

Articles 901 Documents

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MOLECULAR DETECTION OF GENE RESISTANT TO VARIOUS INSECTICIDES IN Aedes aegypti AT BANYUWANGI EAST JAVA USING POLYMERASE CHAIN REACTION Aditya Yudhana; Ratih Novita Praja; Maya Nurwartanti Yunita
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): June
Publisher : Universitas Syiah Kuala

The purpose of this study was to determine the type of insecticides which Aedes aegypti mosquitoes are resistant to, so that other susceptibleinsecticides still can be used. The study was a cross sectional epidemiological study with cluster sampling in Sub-district Wongsorejo,Banyuwangi, Muncar, Tegaldlimo, Kalibaru which is considered sufficient to represent Banyuwangi Regency. Mosquito samples appropriate tothe characteristics were isolated and tested for insecticide-resistance primer namely voltage gated sodium channel (VGSC) to determine thespecific resistance expressed in mosquitoes. The result of resistance test using WHO standard method showed that Aedes aegypti mosquito fromBanyuwangi Regency were resistant to cypermethrin 0.25% and malathion 0.8%. There was a VGSC coding gene with 250 bp band detectedusing polymerase chain reaction (PCR) technique which was associated with the resistance of Aedes aegypti mosquitoes in Banyuwangi Regencyto organophosphate insecticides (malathione) and pyrethroid insecticides (cypermethrin).

EFEK PEMBERIAN HORMON PREGNANT MARE’S SERUM GONADOTROPIN (PMSG) SEBELUM KAWIN TERHADAP GAMBARAN DARAH MERAH INDUK DOMBA SELAMA PERIODE KEBUNTINGAN Andriyanto A; Ridi Arif; Muhammad Darjat Darulfalah; Ganjar Maulana Nugraha; Nastiti Kusomorini; Hera Maheshwari; Wasmen Manalu
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui pengaruh pemberian hormon PMSG sebelum kawin terhadap gambaran darah merah induk domba bunting. Sebanyak 14 ekor induk domba bunting dengan kisaran bobot badan antara 20-25 kg dikelompokkan ke dalam dua kelompok, masing-masing terdiri atas 7 ekor domba. Perlakuan pertama terdiri atas induk domba bunting hasil perkawinan alami tanpa diberikan hormon PMSG (P0) dan perlakuan kedua terdiri atas induk domba bunting yang diberikan 125 IU PMSG (PG 600, Intervet, Holland) sebelum dikawinkan (P1). Sebelum diberikan hormon PMSG, domba penelitian diserentakkan berahinya dengan menyuntikkan 10 mg/ekor prostaglandin (PGF2α, LutalyseTM, Pharmacia Upjohn Company, Pfizer Inc.) sebanyak dua kali dengan interval sebelas hari. Penyuntikan hormon PMSG dilakukan bersamaan dengan penyuntikan hormon PGF2α yang kedua. Domba betina yang estrus dikawinkan secara alami dengan pejantan yang telah diseleksi. Pada hari ke-30 setelah dikawinkan, dilakukan pemeriksaan kebuntingan dan jumlah fetus dengan menggunakan ultrasonografi (USG). Sampel darah untuk pemeriksaan jumlah sel darah merah, kadar hemoglobin, dan nilai hematokrit diambil pada periode kebuntingan bulan ke-1, 2, 3, 4, dan 5. Perlakuan hormon PMSG sebelum perkawinan meningkatkan jumlah sel darah merah, nilai hematokrit, dan kadar hemoglobin induk domba bunting. Peningkatan jumlah sel darah merah, nilai hematokrit, dan kadar hemoglobin terjadi pada periode awal kebuntingan dan menurun pada akhir masa kebuntingan.

KARAKTERISTIK MORFOLOGI ESOFAGUS DAN LAMBUNG BANDIKUT (Echymipera kalubu) (Esophagus and Stomach Morphological Characteristic of Bandicoot Echymipera kalubu (Marsupialia: Peroryctidae)) Ursula Paulawati Maker; Chairun Nisa; Srihadi Agungpriyono
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

The morphological characteristics of the stomach and esophagus of five adults bandicoot (Echymipera kalubu) with average 1.16±0.29 kg in body weight and 38.2±4.76 cm in body length were studied macroscopically, microscopically and histochemically. The bandicoot esophagus were found relatively long about one third of body length. The esophageal glands were identified along the esophagus and the number decreased gradually from cranial to caudal region. In addition of smooth muscle, skeletal muscles were also examined in the external muscle layer of esophagus up to the esophageal junction. The stomach of the E. Kalubu had short lesser curvature and three glandular regions of cardiac, fundic and pyloric glands were observed, respectively. The cardiac glands area was small and concentrated in the cranial part of the stomach. The fundic glands area occupied about half parts of the stomach. Mucous surface epithelial cells, mucous neck cells, parietal cells and chief cells were found in the entire fundic gland. The parietal cells were the most abundant cells in the major curvature distributed from the basal to neck area of the glands, but few in the minor curvature. Chief cells were mostly distributed in the basal gland. The pyloric glands region was observed in caudal part of the stomach. Staining with alcian blue-periodic acid Schift (AB-PAS) showed various distribution and intensity of neutral and acid mucopolysaccharides in the esophageal and stomach mucosa. High concentration of neutral and acid mucopolysaccharides were detected in esophageal and stomach glands with various concentration. Morphological characteristics of the esophagus and stomach were assumed to be related to the digestive processes in the gastrointestinal tract of bandicoot.

ENDOPARASIT CACING PADA ORANGUTAN EX-CAPTIVE DI SUAKA MARGASATWA SUNGAI LAMANDAU KALIMANTAN TENGAH INDONESIA M. Mirsageri; M. Jamaluddin Assidiqi; Umi Cahyaningsih; Risa Tiuria; Zulfiqri Z
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan melakukan identifikasi dan mengetahui persentase orangutan yang terinfeksi endoparasit (cacing) pada orangutan excaptive yang berada di Suaka Margasatwa Sungai Lamandau. Sampel feses orangutan yang diperiksa sebanyak 30 individu yang didapatkan dicamp Gemini dan Siswoyo. Pemeriksaan feses dilakukan secara makroskopis dan mikroskopis. Pemeriksaan mikroskopis dilakukan dengan menggunakan dua metode yaitu metode Ridley dengan larutan sodium acetate, acetic acid, dan formaldehyde (SAF) fiksatif dan metode apung. Hasil pengamatan menunjukkan adanya infeksi cacing gastrointestinal sebesar 56,7%. Hasil penelitian menunjukkan bahwa orangutan positif terinfestasi telur cacing tipe ascarid; trichurid; dan strongiloid masing-masing sebesar 43,3; 16,7; dan 6,7%. Orangutan dapat mengalami infeksi lebih dari satu jenis tipe telur cacing.

VENTRAL MEDIAN AND LATERAL FLANK APPROACH FOR OVARIOHYSTERECTOMY IN CAT Bella Vera; Riki Siswandi; Raden Harry Soehartono
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala

This study was conducted to compare Ventral Median Approach (OHM) and Lateral Flank Approach (OHF) for feline ovariohysterectomy (OH). Fifteen healthy local female cats (Felis catus) with body weights ranged 2-4 kg and aged 1-2 years were divided into two groups, OHM (n= 8) and OHF (n= 7). Prior to OH, the cats were anesthethized using ketamine-xylazine and the sugery was performed by the same operator. The wound scoring showed a significantly higher difference in the OHM group on day 3 and 4 post-surgery. Scores of erythematous, swelling and dehiscence showed no significant differences. The 4Avet pain scale was significantly higher in OHF at the 2, 6, and 12-hours post surgery. The pain response was evaluated using baseline tactile Semmes-Weinstein Monofilaments and was significantly higher in OHF than OHM on day 1 to day 5 post surgery. Blood hematology, blood glucose and wound healing duration did not differ significantly between groups. The OHF approach showed greater post-operative pain than the OHM approach. Wound dehiscence was observed in two cats (25%) in OHM group from day 4 post-operative. The OHM approach showed a greater risk of complications than the OHF group and therefore requires better post-operative care. The OHF group showed a smaller risk of complications but requires better post-operative analgesic therapy.

EVALUATION OF SULFURIC ACID, BARIUM CHLORIDE, AND SEED GERMINATION ASSAY METHODS AS EARLY PREGNANCY DETECTION INSTRUMENTS IN CATTLE Zul Azmi; Muhammad I. Desem; Hastuti Handayani S. Purba; Dwi Endrawati; Faidah Rachmawati; Eni Kusumaningtyas; Didik T. Subekti
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

This study aims to validate and re-evaluate the use of H2SO4, BaCl2, and seed germination assay (SGA) methods to detect early pregnancy in cattle. Validation was carried out in order to find out the actual test performance value, which include accuracy, sensitivity, and specificity.Validation test was done by reacting the urine of pregnant and non-pregnant cattle populations with H2SO4 solution diluted at a ratio of 1 : 4, with 1% BaCl2 solution or through germination of mung bean seeds in Petri dishes. The results of this study indicated that the H2SO4 and BaCl2 methods have poor accuracy (21.88% and 28.13%) with the sensitivity of 81.82% and 100% and specificity of 9.43% and 13.21%, respectively. Meanwhile, the SGA method had a fairly good accuracy (74.55%) with sensitivity and specificity of 45.46% and 81.82%, respectively. Based on these results, the H2SO4, BaCl2, and SGA methods are not recommended for detection of early pregnancy in the field.

Development of Selection Method for Genetic Potential and Adjusment of Nutritional Requirement to Stimulate Gene Expression of Local Meat Chicken M. Aman Yaman; Dasrul Dasrul; Zulfan Zulfan
Jurnal Kedokteran Hewan Vol 3, No 2 (2009): September
Publisher : Universitas Syiah Kuala

This study aimed to evaluate the genetic potential of local meat chickens (LMC) to get the candidate of parent stock through the development of the selection method applied, as well as economical and practical approach and adjusment of nutritional requirement, especially protein and energy balance. Research was divided into 2 groups, where the female and male parent was reared for 2 months and the offspring (F1) was reared for 4 months and selected. The parent was mated by the method of artificial insemination (AI). Method of selection was used on the orientation of the meat chicken production parameters with a positive correlation with the gene expression Diet treatments were formulated by optimal nutrition standards in order to drive maximum primary production of local meat chicken, contained 17, 19, and 21% of protein. The result showed that feeding diet with 19% protein can stimulate optimum growth and food conversion of ALPU male, whereas female growth LMC in line with the increasing availability of protein in the diet, wherein feeding 21% of protein result in optimal growth until age 4 months.

MENCIT (Mus musculus) GALUR BALB-C YANG DIINDUKSIKAN STREPTOZOTOSIN BERULANG SEBAGAI HEWAN MODEL DIABETES MELITUS Erwin e; Etriwati e; Rusli r
Jurnal Kedokteran Hewan Vol 6, No 1 (2012): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui persentase peningkatan sel beta Langerhans pankreas yang mengalami nekrosis setelah diinduksi streptozotosin berulang.Tiga puluh ekor mencit jantan galur Balb-c, umur 12-14 minggu dengan bobot badan 30-40 g dikelompokkan menjadi 2 kelompok perlakuan.Kelompok I (KI)diberikan pelarut streptozotosin danKelompok II (KII) diberikan streptozotosin dengan dosis 40 mg/kg bb dalam 50 mM natrium sitrat buffer pH 4,5.Semua perlakuan diberikan secara intraperitoneum selama 5 hari berturut-turut. Pemeriksaan kadar glukosa darah dan berat badan dilakukan pada hari ke-0, 7, 14, 21, dan 28 sesudah pemberian perlakuan. Hewan percobaan dari masing-masing kelompok dieutanasiasebanyak 2 ekor pada hari ke-7, 14, 21, dan 28 setelah pemberian perlakuan untuk pewarnaan Gomori. Data pengamatan berat badan adalah42,86±2,3 vs 32,78±7,4 gdan kadar glukosa darah puasa 95,89±2,5 vs 255,77±87,60 mg/dl menunjukkan perbedaan yang signifikan (P0,05) antara kedua kelompok perlakuan, sedangkan persentase sel beta yang mengalami nekrosis antara kedua kelompok perlakuan 4,57±0,47 vs 64,11±4,10juga menunjukkan perbedaan yang signifikan (P0,05). Induksi dosis rendah streptozotosin secara berulang dapat menyebabkan peningkatan persentase nekrosis sel beta Langerhans pankreas.

RESISTENSI ANTIBIOTIK DAN DETEKSI GEN PENGODE METHICILLIN RESISTANT Staphylococcus aureus (MRSA) ISOLAT BROILER DI WILAYAH YOGYAKARTA (Resistance of Antibiotics and Detection of Gene Encoding Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Broiler in Yogyakarta) Khusnan K; Dwi Kusmanto; Mitra Slipranata
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

The aims of the research were to evaluate resistance of Staphylococcus aureus against several antibiotics and to detect the gene encoding ofmethicillin resistant Staphylococcus aureus (MRSA) in broiler. Twenty three Staphylococcus aureus isolated from infected broiler in Yogyakartawere used. Genotypic identifications were done based on the occurrence of gene nuc and 23SrRNA. The resistance assay to several antibioticsrevealed that Staphylococcus aureus were resistant to penicillin 78% (18/23), doxycyclin 56% (15/23), gentamicin 26% (6/23), tetracyclin 22% (5/23), erythromycin 13% (3/23), and methicillin 9% (2/23). Detection of gene encoding MRSA on 23 isolats of S. aureus showed that 8 (34.8%) isolates contain gene mecA.Key words: Staphylococcus aureus, broiler, resistant, methicillin, mecA

KAJIAN KEMAMPUAN SELEDRI (Apium graveolens Linn.) SEBAGAI HERBAL ANTISTRES PADA TIKUS WISTAR (Rattus norvegicus) Yusni Y; Ibrahim Puteh
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Tujuan penelitian ini adalah mengetahui, mengkaji, dan menganalisis potensi seledri sebagai herba antistres dan imunomodulator dengan cara menghambat sekresi kortisol plasma pada tikus Wistar (Rattus norvegicus) yang diinduksi kejutan listrik. Penelitian ini merupakan penelitian eksperimental laboratoris, pre and postest with control group design. Sampel yang digunakan adalah 13 ekor tikus Wistar yang dibagi dalam 3 kelompok: P0, kelompok kontrol (kejutan listrik); P1, kejutan listrik + ekstrak seledri 0,14 mg; dan P2, kejutan + ekstrak seledri 0,72 mg. Kejutan listrik dan ekstrak seledri masing-masing diberikan selama 7 hari. Kadar kortisol diukur menggunakan metode enzyme-linked immunosorbent assay (ELISA). Analisis data menggunakan uji analisis varian satu arah. Hasil penelitian menunjukkan bahwa nilai rata-rata kadar kortisol serum kelompok P0 sebelum dan setelah diberikan perlakuan mengalami peningkatan, sedangkan kelompok P1 dan P2 mengalami penurunan (P0= 1,88 vs 3,65; P1= 4,59 vs 2,63 dan P2= 3,00 vs 2,99). Pemberian ekstrak seledri cenderung menurunkan kadar kortisol serum meskipun secara statistik tidak berbeda secara signifikan (P>0,05). Disimpulkan bahwa ekstrak seledri belum sepenuhnya berperan sebagai herbal antistres pada tikus Wistar yang mengalami stres.

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Tongku Nizwan Siregar

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+626517551536

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jkh@usk.ac.id

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Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

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Aceh

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Home Page

OAI Link

Editorial Team

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Contact Name Tongku Nizwan Siregar

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Phone +626517551536

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Editorial Address Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location Kab. aceh besar, Aceh INDONESIA

Abstract

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Abstract

Contact Name
Tongku Nizwan Siregar

Contact Email
jkh@usk.ac.id

Phone
+626517551536

Journal Mail Official
jkh@usk.ac.id

Editorial Address
Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location
Kab. aceh besar,

Aceh

INDONESIA