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Tongku Nizwan Siregar
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INDONESIA
Jurnal Kedokteran Hewan
Published by Universitas Syiah Kuala
ISSN : 1978225X     EISSN : 25025600     DOI : 10.21157
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary
Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 901 Documents
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AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN KARI (Murraya koenigii) TERHADAP Staphylococcus aureus, Escherichia coli, dan Pseudomonas sp. R, Rastina; Sudarwanto, Mirnawati; Wientarsih, Ietje
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2842

Abstract

Penelitian ini bertujuan mengetahui aktivitas antibakteri, konsentrasi efektif, dan pengaruh peningkatan konsentrasi ekstrak etanol daun kari (Murraya koenigii) terhadap daya hambat pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Pseudomonas sp. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut etanol 96%. Pengujian aktivitas antibakteri menggunakan metode difusi agar. Parameter yang diukur adalah besarnya diameter daya hambat yang terbentuk di sekitar kertas cakram. Hasil uji aktivitas antibakteri dianalisis dengan metode one way anova dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa konsentrasi ekstrak 12,5; 25; dan 50% telah memberikan aktivitas daya hambat pertumbuhan bakteri uji. Konsentrasi efektif yang dapat menghambat bakteri Staphylococcus aureus dan Escherichia coli pada konsentrasi 50%, sedangkan bakteri Pseudomonas sp. pada konsentrasi 12,5; 25; dan 50%. Peningkatan konsentrasi ekstrak daun kari menunjukkan semakin luas diameter zona hambat pertumbuhan bakterinya. Penghambatan yang terjadi pada bakteri Staphylococcusaureus, Escherichia coli, dan Pseudomonas sp. tersebut, membuktikan bahwa daun kari mengandung senyawa aktif yang bersifat antibakteri, seperti flavonoid, fenol, alkaloid, dan saponin.
ANTISPERMATOGENIC ACTIVITY OF ETHANOLIC EXTRACT OF CALINA PAPAYA LEAVES ON SEMINIFEROUS TUBULES WISTAR RATS Setiawan, Haris; Wulandari, Sri Wijayanti; Agustina, Erlita Devi
Jurnal Kedokteran Hewan Vol 15, No 1 (2021): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i1.18435

Abstract

Indonesia is a country with high birth rate and the rate continues to increase each year. Methods to reduce high birth rate are needed, especially herbal based contraceptives. This research aims to study the possibility of Calina papaya leaf ethanolic extract as an antispermatogenic agent which hopefully could be expanded into natural antifertiliy drug candidate. Research was done using 24 white male Wistar rats divided into four groups. Dosages were given as follows: 0 mg/Kg W (control group), 100 mg/Kg W (P1), 200 mg/Kg W (P2) and 300 mg/Kg W (P3). Treatment was given in 30 days period. Rats were sacrificed on Day 31 and the testes were taken. Testes were then made into histological specimen using paraffin block and stained using Haematoxylin-Eosin. The diameter and surface area of seminiferous tubules, lumen surface area, and spermatogenesis index were then observed. Results showed that there is a significant difference (p0.05) on lumen surface area and spermatogenesis index between control and treatment groups. It is also found that the optimum dosage given to attain the desirable effect is 300 mg/Kg W. There is no significant difference found on surface area and diameter of tubulus seminiferous between control and treatment group (p0.05). Based on the results, papaya leaf (Calina variety) ethanolic extract has the potential a natural antispermatogenic with the optimum dose of 300 mg/Kg W.
IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF BOVINE HERPERVIRUSES (BoHV) DNA TERMINASE PARTIAL GENE IN ACEH CATTLE Prayitno, Lilik; Saepuloh, Uus; Mayasari, Ni Luh Putu Ika; Faisal, Faisal; Ayuningsih, Ellis Dwi; Pamungkas, Joko Pamungkas
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.8024

Abstract

Bovine Herpesvirus (BoHV) is a member of Herpesviridae family that acts as pathogenic virus causing infectious bovine rhinotracheitis (IBR) among cattles, resulting in economic loss for cattle industry. BoHV-1 infection in cows is closely related to abortion, respiratory infection, reduced milk production, infertility, and low birth weight. The aim of this study was to identify and characterize the molecular of BoHV-1 and other virus types, as well as the possible presence of other Herpesviridae family using PCR to amplify DNA terminase gene. Four out of 210 nose swab samples were positive for herpes virus on DNA terminase gene. Further characterization of samples showed 99-100% similarity to BoHv-1 and BoHV-6 sequence. Genetic distance between genera BoHV-1 and BoHV-6 is 0.518 and within genera was 0.001 and 0.044. According to phylogenetic tree analysis of DNA terminase gene, the analyzed sequence clustered into 2 genera, namely Varicellovirus which is identical to BoHV-1 and Macavirus which is identical to BoHV-6. The study provides scientific information on molecular characteristics of Herpesviridae family, especially BoHV-1 which is prevalent in Indonesia with the highest density in the central ranches in Aceh province
PRODUKSI EMBRIO KUCING SECARA IN VITRO DARI SPERMATOZOA HASIL PRESERVASI MELALUI FERTILISASI MIKRO Eriani, Kartini; Sukra, Yuhara; Boediono, Arief; Djuwita, Ita; Sumarsono, Sony Heru
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.563

Abstract

Penelitian ini bertujuan mengetahui motilitas dan kemampuan memfertilisasi sperma kucing yang berasal dari epididimis yang disimpanpada suhu 4 C. Epididimis disimpan dalam media phosphate buffer saline (PBS) pada suhu 4 C selama 1, 3, dan 6 hari. Viabilitas spermatozoa diamati dengan pewarnaan hoechst-propidium iodine. Fungsi biologis spermatozoa dievaluasi melalui teknik kultur in vitro dengan fertilisasi mikro dan perkembangan embrio di dalam media kultur CR1aa. Hasil penelitian menunjukkan persentase spermatozoa hidup pada hari ke-1, 3, dan 6 penyimpanan masing-masing adalah 81,0; 71,7; dan 70,7% (duktus deferens), 84,0; 81,2; dan 63,2% (kauda epididimis), 84,0%; 75,0; dan 74,7% (korpus epididimis). Persentase pronukleus (PN) yang didapat dengan teknik intra cytoplasmic sperm injection (ICSI) menggunakan spermatozoa epididimis pada hari ke-1, 3, dan 6 hari penyimpanan masing-masing adalah 8,0; 10,0; dan 5,9%. Preservasi epididimis pada suhu 4 C dalam PBS dapat digunakan untuk fertilisasi dan menghasilkan embrio kucing secara in vitro.
PROFIL HORMON ESTROGEN DAN PROGESTERON PADA SIKLUS BERAHI KAMBING LOKAL (Estrogen and Progesterone Profile in Aceh Local Goat Estrous Cycle ) Siregar, Tongku Nizwan
Jurnal Kedokteran Hewan Vol 3, No 2 (2009): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v3i2.3827

Abstract

The aim of this research was to find out the normal estrogen and progesterone profile in local goat estrous cycle. Five female Aceh local goats which the criteria clinically in good condition, have been giving birth before, 1.5-3.0 years old, and showed minimal twice regularly estrous cycle. All samples were synchronized with 0.5 ml cloprostenol (Estrone, Bioveta) using randomly infection pattern. The data obtained were analyzed descriptively. Blood serum was collected for examination of estrogen and progesterone content on 0, 7, 14, and 21 day of estrous cycle (day 0 of estrous cycle). Hormone analysis was conducted using ELISA. The results showed that estrogen and progesterone profile on day 0, 7, 14, and 21 were 62.21; 28.61; 29.84; 79.89 pg/ml and 0.0; 8.6; 14.7; 0.0 ng/ml respectively.Keywords: estrogen, progesterone, estrous cycle, Aceh local goats
EFEKTIVITAS PENGGUNAAN VAKSIN DNA DALAM PAKAN PADA IKAN MAS YANG DIINFEKSI KOI HERPESVIRUS Nuryati, Sri; Khodijah, Siti; A, Alimuddin; Setiawati, Mia
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2784

Abstract

Penelitian ini bertujuan menentukan frekuensi pemberian vaksin DNA koi herpesvirus (KHV) yang menghasilkan kelangsungan hidup tinggi pada ikan mas yang diinfeksi KHV. Frekuensi pemberian vaksin yaitu vaksinasi satu kali seminggu (K1), vaksinasi dua kali seminggu (K2), vaksinasi tiga kali seminggu (K3), ikan tidak divaksin dan diuji tantang KHV (K4), dan ikan diinjeksi dengan larutan phosphate buffered saline (K5). Pemberian pakan dilakukan 2 kali sehari secara satiasi.Mortalitas pada K1; K2; K3; dan K4 masing-masing adalah 33,335,77; 20,0010,00; 6,67 5,77; dan 43,335,77%, sedangkan kelangsungan hidup relatif (RPS) K1; K2; dan K3 masing-masing adalah 23,0713,32; 53,8423,07; 84,6013,32%. Disimpulkan bahwa pemberian pakan mengandung vaksin DNA anti-KHV dengan frekuensi tiga kali dalam satu minggu efektif meningkatkan kelangsungan hidup ikan mas yang diinfeksi KHV.
EFFECT OF ETHANOLIC NEEM (Azadirachta indica) LEAVES EXTRACT ON DEVELOPMENT OF SPERMATID AND ANDROGEN RECEPTOR EXPRESSION IN THE TESTIS OF RABBIT Aulia, Usma; Wahyuni, Sri; Gholib, Gholib; Dasrul, Dasrul; Adam, Mulyadi; Rahmi, Erdiansyah; Hamzah, Abdullah
Jurnal Kedokteran Hewan Vol 17, No 1 (2023): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i1.30042

Abstract

The objective of this study was to determine the effect of ethanol extract of neem (Azadirachta indica) leaves on the number of spermatids and androgen receptor expression in the testicular tissue of New Zealand White (NZW) rabbits. Twelve adults male NZW rabbits with body weight (BW) 2-2.5 kg were divided into three treatment groups (n = 4), namely P1 (control group) given 0 mg/kg BW, P2 and P3 were given 200 mg/kg BW and 300 mg/kg BW of neem leaves ethanol extract respectively. The extract was administered orally using a gastric tube for 52 days. At the end of the treatment, the rabbits were terminated and the testes were collected to be processed into histological preparations and stained with Periodic Acid Schiff (PAS) staining to detect the number of spermatid and Immunohistochemical (IHC) staining to detect androgen receptor expression. The results showed that neem leaves extract administered at P3 rabbits significantly decreased the number of spermatids and androgen receptor expression in myoid peritubular cells and connective tissue P0.05), but not significantly different in the Leydig cells and blood vessels (P0.05) of testicular tissue. The conclusion of this study is that the ethanol extract of neem leaves at a dose of 300 mg/kg BW can be used as a contraceptive candidate compared to a dose of 200 mg/kg BW.
SINGLE BULB GARLIC (Allium sativum) EXTRACT IMPROVE SPERM QUALITY IN HYPERLIPIDEMIA MALE MICE MODEL Qadariah, Nosi; Lestari, Sri Rahayu; Rohman, Fatchur
Jurnal Kedokteran Hewan Vol 14, No 1 (2020): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i1.13562

Abstract

The aim of this research was to find out the influence of single bulb garlic on sperm quality improvement in hyperlipidemia model of male mice. Male mice (Balb-C, 12 weeks, bw 215 g) were given high fat diet for 45 days until their body weight achieved 455 g. The mice were then divided into six groups. The mice in the first group (control group (N)) was fed with 7 g higrow pokphan 551 per day. The mice in the second group (control negative group) was fed with 7 g high fat diet (HFD), while the mice in the third group (control positive group) were treated with statin at dose of 0.91 mg. The mice in groups 4, 5, and 6 were treated with single bulb garlic extract at dose of 125 mg/kg bw, 250 mg/kg bw, and 500 mg/kg bw, respectively. On the 31st day, the male were dissected and cauda epididymis was taken and chopped in a sterile phosphat buffer saline (PBS) and observation conducted on sperm quality consisted of sperm count, sperm motility and sperm normality. The results indicated a significant increase in sperm count, motility and normality in mice treated with single bulb garlic extract at dose of 250 mg/kg bw.
Mineral Level Status of Copper, Magnesium, and Iron in Local Goat Serum Related to Keeping Pattern System Delima, Mira
Jurnal Kedokteran Hewan Vol 2, No 1 (2008): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v2i1.9777

Abstract

This research was conducted to find out the mineral level status of Cu, Mg, and Fe in blood serum of local goats which are reared with different methods. Blood serum was taken from 15 male and 15 female goats, 15-18 month old. The goats were divided into three groups which consist of 5 males and 5 females. Three methods of caging in which most commonly conducted by local people were selected as treatments, i.e. 1) Goats are caged only at night, while in daytime (around 10.00-12.00 WIB) they are discharged freely therefore they could foraging; 2) Goat are caged at night, while in daytime (around 10.00-18.00 WIB) they are bound with rope at which they could foraging around and, the rope would be removed several times in that particular daytime; 3) Goats are released freely all day long, but they are provided with kind of place as a cage, under the house or under the tree. Blood serum was taken from every unit sample. This research revealed that there was no significant different among methods of caging on the level rates of Cu, Mg, and Fe in local goat serum. The level rates of Cu, Mg, and Fe in goats serum that was found from the research were in normal range.
PROFIL DISTRIBUSI INOS DAN KADAR NO PANKREAS TIKUS DIABETES MELITUS HASIL INDUKSI MLD-STZ PASCA PEMBERIAN EKSTRAK ETANOL TEMUGIRING (Curcuma heyneana) Lukiati, Betti; a, Aulanniam; Darmanto, Win
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.343

Abstract

Penelitian ini bertujuan menguji pengaruh ekstrak etanol temugiring terhadap distribusi inducible nitric oxide synthase (iNOS) dan kadar nitrogen oksida (NO) pada pankreas tikus diabetes hasil induksi multiple low dose-streptozotocin (MLD-STZ). Tiga macam dosis ekstrak etanol temugiring, yaitu 36, 72, dan 108 mg/kg bobot badan diberikan secara oral untuk terapi tikus diabetes hasil induksi MLD-STZ. Data distribusi iNOS dan kadar NO dianalisis dengan analisis varians jalur tunggal yang dilanjutkan dengan uji Duncan (=0,05). Hasil analisis menunjukkan bahwa ekstrak etanol temugiring berpengaruh sangat nyata (P0,01) terhadap penurunan distribusi iNOS dan kadar NO pada pankreas tikus diabetes hasil induksi MLD-STZ. Dosis 72 mg/kg bobot badan ekstrak etanol temugiring merupakan dosis optimal untuk terapi tikus diabetes hasil induksi MLD-STZ. Disimpulkan bahwa ekstrak etanol temugiring dapat menurunkan distribusi iNOS dan kadar NO pankreas tikus penderita diabetes melitus.

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