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Berita Biologi
Published by Badan Riset dan Inovasi Nasional
ISSN : 23378751     EISSN : 23378751     DOI : https://doi.org/10.55981/berita_biologi
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Berita Biologi is the journal features articles showcasing advancements in biological research and related sciences in Indonesia. Berita Biologi comprises original scientific papers presenting research findings, short communications, and reviews that are unpublished or not under consideration elsewhere. Covered topics are expected to introduce novel aspects or information.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 126 Documents
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PROFIL BIONOMIK VEKTOR POTENSIAL DENGUE DI KECAMATAN SUMBERSARI, KABUPATEN JEMBER Wathon, Syubbanul; Ali Maziun, Iffa; Oktarianti, Rike; Senjarini, Kartika
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.9215

Abstract

Kabupaten Jember menempati urutan ketiga tertinggi kasus Demam Berdarah Dengue (DBD) di Jawa Timur, dengan angka tertinggi di Kecamatan Sumbersari. Beberapa upaya untuk memberantas kasus DBD telah dilakukan, misalnya pemberantasan sarang nyamuk (3M plus), fogging, penggunaan larvasida, “Gerakan Jumantik” namun masih ada kasus DBD khususnya di daerah endemik. Upaya penanggulangan DBD memerlukan studi bionomik untuk mencapai strategi yang efektif dalam program pengendalian vektor. Tujuan penelitian ini adalah untuk mengetahui bionomik vektor potensial DBD di wilayah endemik DBD Kecamatan Sumbersari, Jember, yang meliputi: identifikasi morfologi, proporsi jenis dan perilaku istirahat vektor nyamuk, serta karakteristik habitat dan kepadatan larva vektor nyamuk. Penelitian ini dilakukan dengan pendekatan metode purposive sampling pada beberapa lokasi di Kecamatan Sumbersari pada bulan September 2020. Koleksi sampel larva Aedes dilakukan dengan single larva methods sedangkan nyamuk dikoleksi dengan metode resting collection di dalam maupun luar rumah. Hasil penelitian menunjukkan bahwa proporsi Ae. aegypti (91%) lebih tinggi dibandingkan dengan Ae. albopictus (9%). Perilaku istirahat Ae. aegypti menunjukkan karakter endofilik, sedangkan Ae. albopictus lebih bersifat eksofilik. Puncak aktivitas istirahat Aedes spp. terjadi pada pukul 09.00–10.00 WIB. Karakteristik tempat perindukan Aedes spp. ditemukan di kontainer air berupa bak mandi (62%, dibandingkan jenis ember dan pot), berbahan semen (46%, dibandingkan bahan plastik dan keramik), berwarna gelap (77%, dibandingkan dengan berwarna terang) dan terletak di dalam ruangan (85%, dibandingkan dengan di luar ruangan). Kepadatan larva berdasarkan indek House Indicator di Kecamatan Sumbesari menunjukkan angka sebesar 30%. Berdasarkan tabel resiko penularan DBD, maka resiko penularan DBD pada Kecamatan Sumbersari termasuk dalam kategori tinggi.
ANALISIS OTENTIKASI DAN FITOKIMIA TUMBUHAN BROTOWALI ASAL SABU RAIJUA Ledo, Mellissa Erlyn Stephanie; Rupidara, Anggreini; Ballo, Apriliana; Nge, Sonya T; Ngede, Alfandi
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

 Dua jenis tumbuhan berkhasiat obat dari Sabu Raijua yang memiliki nama daerah Puri raho dan Loro wawi eddu  memiliki kemiripan morfologi dengan tumbuhan brotowali, namun belum dilakukan identifikasi taksonomi dan kandungan senyawa fitokimianya. Tujuan penelitian ini adalah untuk mengidentifikasi taksonomi Puri raho dan Loro wawi eddu serta menganalisis kandungan fitokimianya (kuersetin). Penelitian ini terdiri atas 2 tahap yaitu identifikasi morfologi dan analisis penanda DNA ITS2 dilanjutkan dengan identifikasi kandungan fitokimia (kuersetin) pada ekstrak batang  menggunakan metode kromatografi lapis tipis (KLT) dan metode spektrofotometri. Identifikasi taksonomi tumbuhan Puri raho dan Loro wawi eddu menggunakan buku kunci determinasi menunjukkan bahwa kedua tumbuhan ini termasuk dalam ordo Ranunculales, selanjutnya perbandingan akar, batang dan daun dengan spesies dari genus Tinospora lainnya menunjukkan kemiripan antara Puri Raho dengan Tinospora cordifolia dan Loro wawi eddu dengan Tinospora macrocarpa namun kedua tumbuhan asal Sabu Raijua ini tidak memiliki bunga, buah dan biji sehingga dilanjutkan dengan identifikasi molekuler menggunakan penanda DNA ITS2 yang menunjukan tumbuhan Puri raho memiliki persentase identitas DNA ITS2 sebesar 100% dengan Tinospora crispa voucher Chen ZD s.n. (PE) Sequence ID: KY365661.1, sedangkan Loro wawi eddu menunjukkan persentase identitas DNA ITS2 sebesar 99,17 % dengan Tinospora smilacina voucher Gray 8798 (MO) Sequence ID: KY365675.1. Uji kualitatif kandungan kuersetin menunjukkan hasil positif dengan munculnya bercak pada plat KLT dengan Rf 0,91 pada sampel dan larutan standar kuersetin, sedangkan hasil uji spektrofotometri menunjukkan kandungan kuersetin pada ekstrak batang Puri raho sebesar 99,24 ppm dan 78,08 ppm pada ekstrak batang Loro wawi eddu.
KEANEKARAGAMAN DAN DISTRIBUSI HERPETOFAUNA PADA BERBAGAI FUNGSI HUTAN DI KESATUAN PENGELOLAAN HUTAN (KPH) KAPUAS HULU UTARA, KALIMANTAN BARAT Rofi'i, Ikhwanudin; Subeno; Frita Kusuma Wardhani; Rika Bela Rahmawati
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.10280

Abstract

Kesatuan Pengelolaan Hutan (KPH) Kapuas Hulu Utara adalah salah satu wilayah pengelolaan hutan berbasis tapak yang terbagi dalam enam wilayah kerja berdasarkan fungsi hutan. Berbagai kegiatan yang dilakukan di kawasan tersebut dapat memengaruhi kehidupan satwa, salah satunya yaitu herpetofauna yang merupakan bio-indikator kesehatan lingkungan. Penelitian ini bertujuan untuk mengetahui keanekaragaman dan distribusi spasial spesies herpetofauna pada berbagai fungsi hutan di KPH Kapuas Hulu Utara. Penelitian dilakukan pada tiga fungsi hutan yang dominan di KPH Kapuas Hulu Utara, yaitu hutan lindung, hutan produksi, dan hutan produksi terbatas. Data keanekaragaman spesies herpetofauna diambil menggunakan metode VES rancangan transek atau jalur (VES with transect design) sepanjang ± 1 km dan dianalisis dengan menggunakan Indeks Shannon, sedangkan pola distribusi herpetofauna diketahui dengan menggunakan Indeks Dispersi dan Indeks Green. Berdasarkan hasil penelitian, diperoleh 86 spesies herpetofauna yang terdiri dari 36 spesies amfibi dan 50 spesies reptil. Indeks diversitas di hutan lindung tertinggi dibandingkan fungsi hutan lainnya, dengan nilai 3,01 sedangkan yang terendah pada fungsi hutan produksi dengan nilai diversitas sebesar 2,47. Herpetofauna di KPH Kapuas Hulu Utara memiliki pola distribusi mengelompok dengan nilai indeks dispersi sebesar 11,87 dan indeks green sebesar 0,36.  
THE EFFECTIVENESS OF Eucheuma cottonii EXTRACT AGAINST DENGUE VECTOR LARVAE AS AN EVALUATION FOR NATURAL LARVICIDE DEVELOPMENT Setyaningrum, Endah; Novrianda, Nabila Tias; Mumtazah, Dzul Fithria; Rosa, Emantis
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.11236

Abstract

Dengue fever (DHF) is a tropical disease transmitted by the Aedes aegypti mosquito. Prolonged use of synthetic chemical larvicides such as Abate® has led to resistance and environmental impacts. Therefore, an alternative natural larvicide that is more environmentally sustainable is needed. Eucheuma cottonii is a red alga known to contain bioactive compounds such as flavonoids, alkaloids, terpenoids, saponins, and tannins that can potentially inhibit mosquito larvae development. This study aims to evaluate the effectiveness of the ethanol extract of E. cottonii on the mortality of Aedes aegypti instar III larvae as a basis for the development of natural biolarvicides. The study used a completely randomized design (CRD) with six treatments: four extract concentrations (50 ppm, 100 ppm, 200 ppm, and 300 ppm), one positive control (Abate®), and one negative control (water). Each test unit contained 20 third-instar larvae with four replicates. Observations were made 4, 8, 24, 48, and 72 hours after treatment. The highest larval mortality was achieved at a 200 ppm concentration of 13.25 ± 3.94. The LC₅₀ value at 24 hours was 42.35 ppm, while the LT₅₀ reached 52.77 hours, indicating a slow and less effective larvicidal effect at the test concentration. Although the ethanol extract of E. cottonii contains bioactive compounds, its effectiveness as a larvicide against Aedes aegypti larvae is still relatively low. These results indicate that E. cottonii is not optimally used alone as a bio-larvicide, but still has the potential to be further developed through improved extraction methods, increased concentrations, or combination formulations. This study provides a foundation for creating sustainable and environmentally friendly plant-based larvicides.
EXPRESSION OF HSA-MIR-22-3P IN THE URINE OF PROSTATE CARCINOMA PATIENTS AS A NON-INVASIVE BIOMARKER Prasetyo, Angga Dwi; Danarto, R.; Haryana, Sofia Mubarika; Astuti, Indwiani
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.11311

Abstract

Prostate carcinoma is one of the prostate diseases with the highest prevalence in men. Many factors cause the disease; some are androgen receptor disorder, mutation of genes, age, epigenetics, and environment. Currently, the detection of the disease is done by Prostate-Specific Antigen (PSA), Transurethral Resection of Prostate (TURP), and Digital Rectal Examination (DRE) tests; all of which are invasive to the patients. The microRNA that exists in urine exosomes can be used to detect non-invasive prostate carcinoma.  Hsa-miR-22-3p with Gleason Score. This study aimed to examine the expression potential of Hsa-miR-22-3p in urine samples of prostate carcinoma exosomes as a non-invasive biomarker to determine the correlation between the expression of Hsa-miR-22-3p and the value of the Gleason Score. This study is of cross-sectional observational analysis. Urine samples were obtained from RSUP dr. Sardjito Yogyakarta dan RSUP dr. Soeradji Tirtonegoro. The exosome isolation was then carried out, followed by RNA isolation, cDNA synthesis, and quantification using qRT-PCR. Based on the result of the study, there was a decrease in the expression of Hsa-miR-22-3p by 6.6 times in prostate carcinoma; there was a significant difference between the samples of prostate carcinoma and healthy individuals (P = 0,031), and there was a correlation between the expression level of Hsa-miR-22-3p and the value of Gleason Score. Therefore, Hsa-miR-22-3p has the potential to be used as a biomarker for prostate carcinoma patients.
ANTIOXIDANT ACTIVITY PROFILING OF RED BAJAKAH TAMPALA (Spatholobus littoralis Hassk) FRACTIONS USING THE DPPH RADICAL SCAVENGING METHOD Amanda, Nathasya Gracya; Sari, Ghani Nurfiana Fadma; Novita, Mega; Marlina, Dian
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.11322

Abstract

Indonesia’s rich biodiversity harbors numerous plants with medicinal properties, including Red Bajakah Tampala (Spatholobus littoralis Hassk.), which has long been used in traditional medicine for its therapeutic benefits. Despite its promising ethnomedicinal reputation, scientific validation of its antioxidant potential remains limited. This study aims to evaluate the antioxidant properties of Red Bajakah Tampala and identify key bioactive compounds responsible for its bioactivity. The plant's antioxidant capacity was assessed using the DPPH assay, and the chemical composition was analyzed through phytochemical screening and thin-layer chromatography (TLC). The ethyl acetate fraction exhibited the strongest antioxidant activity, with an IC₅₀ value of 12.87 ppm, followed by the n-hexane fraction (24.08 ppm) and the water fraction (57.23 ppm). Phytochemical screening revealed the presence of flavonoids, tannins, triterpenoids, phenols, and saponins, which contribute to the plant's antioxidant effects. These findings provide scientific evidence supporting the traditional use of Red Bajakah Tampala as a natural antioxidant, with implications for its potential use in therapeutic and cosmetic applications. Further research into the molecular mechanisms and practical applications of Bajakah-based formulations is recommended.
GROWTH OF NYPA PALM WORMS (Namalycastis rhodochorde) JUVENILES FED ON NYPA PALM FRONDS FERMENTED BY CELLULOLYTIC BACTERIA NrLtC4 AND NrLtG2 Hosiana Patricia Sheren; Ari Hepi Yanti; Tri Rima Setyawati; Rikhsan Kurniatuhadi
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.11352

Abstract

Nypa palm worms (Namalycastis rhodochorde: Polychaetes) play important ecological roles as detritivores of organic matter in mangrove ecosystems, bioindicators of water pollution, and sediment stabilizers in estuarine environments. N. rhodochorde also shows potential for use in fermented feed-based aquaculture due to its high nutritional value as a natural food source for aquatic organisms. Cellulolytic lactic acid bacteria isolated from the coelomic fluid and gastrointestinal tract of N. rhodochorde, identified as isolates NrLtC4 and NrLtG2, have potential as starter cultures for fermenting niypa palm fronds, which are used as feed in nypa palm worm cultivation. This study aimed to evaluate the growth of juvenile N. rhodochorde fed nypa palm fronds fermented by the cellulolytic bacteria NrLtC4 and NrLtG2. Juveniles were maintained under consistent stocking density, container size, and water volume, with three treatments arranged in a completely randomized design: unfermented nypa palm fronds (control), nypa palm fronds fermented with isolate NrLtC4, and nypa palm fronds fermented with isolate NrLtG2. The results demonstrated that fermented feed significantly influenced both the number of body segments [p = 0.00021 and 0.0028 (p < 0.05)] and body length [p = 0.0020 and 0.0035 (p < 0.05)] of the juveniles. Juveniles fed nypa palm fronds fermented with strain NrLtC4 showed increases of 29.6% in segment number and 58.5% in body length, while those fed fronds fermented with strain NrLtG2 exhibited increases of 36.6% and 63.9%, respectively. The highest juvenile survival rate was observed in the NrLtC4 fermented feed group at 72%, followed by 69.33% in the NrLtG2 group. These findings suggest that bacterial isolates NrLtC4 and NrLtG2 have promising potential as a consortium feed to enhance the growth of nypa palm worms in aquaculture.
ISOLASI DAN KARAKTERISASI MOLEKULAR 16S rRNA PROBIOTIK DARI MINUMAN FERMENTASI KULIT BIJI KOPI SEBAGAI ANTI INFEKSI PENYAKIT TROPIS Maulidayanti, Sharfina; Andini, Futri; Situmorang, Ing Mayfa Br; Tanjung, Asbar; Ningrum , Isra Jannatin
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.11939

Abstract

Penyakit infeksi tropis yang disebabkan oleh bakteri Escherichia coli dan Salmonella typhi menjadi tantangan besar dalam dunia kesehatan, terutama dengan meningkatnya kasus resistensi antibiotik. Penelitian ini bertujuan untuk mengeksplorasi potensi probiotik dari minuman fermentasi kulit biji kopi (Coffea sp.) sebagai alternatif alami untuk menghambat pertumbuhan bakteri patogen tersebut. Isolasi dan karakterisasi fenotipik dilakukan terhadap bakteri asam laktat (BAL), meliputi pengamatan morfologi koloni, pewarnaan Gram, uji katalase, dan uji motilitas. Selain itu, dilakukan uji ketahanan terhadap pH rendah dan garam empedu, serta uji daya hambat terhadap E. coli dan S. typhi. Hasil menunjukkan bahwa isolat BAL yang diperoleh memiliki ciri khas, yaitu morfologi koloni bulat putih, bersifat Gram positif, non-motil, dan katalase negatif. Isolat juga mampu bertahan pada kondisi ekstrem, yakni pH 3 hingga 7 serta garam empedu 3%. Uji daya hambat menunjukkan kemampuan antibakteri yang baik, dengan zona hambat terhadap E. coli sebesar 27,25 mm dan terhadap S. typhi sebesar 22,5 mm, yang termasuk dalam kategori susceptible hingga intermediate. Analisis genotip melalui sekuensing gen 16S rRNA menunjukkan bahwa isolat memiliki persentase homologi sebesar 98,7% dengan Lacticaseibacillus rhamnosus, mengidentifikasikannya sebagai spesies tersebut. Dengan demikian, Lacticaseibacillus rhamnosus dari fermentasi kulit biji kopi menunjukkan potensi sebagai kandidat probiotik alami, yang dapat digunakan dalam pengendalian infeksi bakteri tropis seperti E. coli enteropatogenik dan S. typhi.
KEANEKARAGAMAN JENIS BAMBU DAN STUDI ETNOBOTANINYA DI KECAMATAN LAMPASIO, KABUPATEN TOLITOLI, SULAWESI TENGAH Muthmainnah, Muthmainnah; Pitopang, Ramadanil; Rachman, Imran; Yusran , Yusran; Ariyanti, Ariyanti
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.11997

Abstract

Informasi mengenai keanekaragaman dan pemanfaatan etnobotani bambu di Kecamatan Lampasio, Kabupaten Tolitoli, masih terbatas, meskipun wilayah ini memiliki potensi sumber daya bambu yang tinggi dengan sebaran relatif merata di hampir semua desa. Penelitian ini bertujuan menganalisis jenis-jenis bambu dan bentuk pemanfaatannya oleh masyarakat. Penelitian dilakukan pada Februari–April 2025 menggunakan metode jelajah untuk eksplorasi jenis, serta studi etnobotani dengan purposive sampling dan snowball sampling. Nilai kepentingan budaya setiap jenis dianalisis dengan Index of Cultural Significance (ICS). Hasil penelitian menemukan sembilan jenis bambu yaitu Bambusa vulgaris var. striata (Lodd.ex Lindl), Bambusa vulgaris var. vulgaris Schard, Schizostachyum brachycladum Kurz, Dendrocalamus strictus (Roxb). Nees, Gigantochloa pseudoarundinacea (Steud.) Widjaja, Gigantochloa atroviolacea Widjaja, Schizostachyum lima (Blanco) Merr, Bambusa glaucophylla Widjaja, dan Bambusa multiplex (Lour.) Jenis bambu dengan nilai ICS tertinggi adalah Schizostachyum brachycladum Kurz (ICS = 516), yang dimanfaatkan luas sebagai bahan pangan, pagar, kayu bakar, wadah nasi lemang, tali, dan anyaman. Jenis ini perlu difokuskan pada konservasi dan budidaya untuk menjaga ketersediaannya. Sebaliknya, Bambusa glaucophylla Widjaja dan Bambusa multiplex (Lour.) memiliki nilai ICS terendah (ICS = 12), mencerminkan pemanfaatan yang terbatas. Kedepan, kedua jenis ini berpotensi dikembangkan melalui eksplorasi pemanfaatan baru dan peningkatan kesadaran masyarakat terhadap nilai tambahnya.
BEYOND THE CONTROVERSY: SOIL MICROBIOME DYNAMICS IN OIL PALM VS. NATURAL PEAT ECOSYSTEMS Widiastuti, Happy; Pulunggono, Heru; Siswanto, Siswanto; Mubarak, Husni; Anwar, Syaiful; Taniwiryono, Darmono; Sumawinata, Basuki; Sabiham, Supiandi; Permatasari, Galuh
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.12564

Abstract

The increasing global demand for palm oil has driven the expansion of oil palm cultivation into peatland areas, raising concerns over environmental sustainability. This study investigates the impact of different land uses on soil microbial diversity and physicochemical properties by comparing three ecosystems: peat soil planted with oil palm (GS), peat soil with natural fern vegetation (GNS), and mineral soil cultivated with oil palm (MS). Physicochemical parameters including organic carbon, cellulose, and lignin content were analyzed, followed by 16S rRNA gene sequencing (V3-V4 regions) using the Illumina MiSeq platform. Sequencing data were processed using the QIIME2 pipeline and classified with the SILVA v.138 database. Results show that while MS had the highest microbial diversity, GS and GNS also supported diverse bacterial communities. Organic carbon, cellulose, and lignin were more abundant in GNS and GS than in MS, reflecting the influence of vegetation on soil composition. Dominant bacterial phyla across all sites included Proteobacteria, Actinobacteria, Firmicutes, and Acidobacteria. These findings suggest that oil palm cultivation on peatlands, when properly managed, does not necessarily reduce microbial diversity. Environmental factors such as vegetation cover, soil type, and water table levels play key roles in shaping microbial communities in tropical soils.

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