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Berita Biologi
Published by Badan Riset dan Inovasi Nasional
ISSN : 23378751     EISSN : 23378751     DOI : https://doi.org/10.55981/berita_biologi
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Berita Biologi is the journal features articles showcasing advancements in biological research and related sciences in Indonesia. Berita Biologi comprises original scientific papers presenting research findings, short communications, and reviews that are unpublished or not under consideration elsewhere. Covered topics are expected to introduce novel aspects or information.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 126 Documents
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EFEK HEPATOPROTEKTIF EKSTRAK ETANOL BANDOTAN (Ageratum conyzoides L.) TERHADAP TIKUS WISTAR (Rattus norvegicus) DIINDUKSI PARAQUAT Listina, Jelia Enggal Listina; Nurazizah, Intan Faya Nurazizah; Apriyanti, Apriyanti; Rohamantin, Dita Rohamantin; Basalamah, Lubna Basalamah; Setiawan, Haris
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.5613

Abstract

Indonesia menduduki peringkat ke-3 di dunia sebagai negara dengan penggunaan pestisida setelah Brazil dan Amerika Serikat, tercatat pada tahun 2021 penggunaan pestisida mencapai 238 kT. Petani menggunakan pestisida yang mengandung paraquat dichloride. Paparan dari paraquat dichloride ke dalam tubuh akan mengakibatkan detoksifikasi di hepatosit sehingga hati rentan mengalami kerusakan yang ditandai dengan meningkatkan kadar SGOT dan SGPT darah. Tanaman bandotan (Ageratum conyzoides L.) merupakan salah satu gulma yang sebelumnya telah dibuktikan bahwa tanaman bandotan memiliki sifat alelokimia yang mampu meningkatkan kandungan fenolik yang bersifat sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak tanaman bandotan (Ageratum conyzoides L.) terhadap perubahan histologi pada organ hati dan ginjal tikus yang terpapar paraquat dichloride. Penelitian menggunakan 24 ekor Tikus Wistar jantan, dengan 4 perlakuan yang terdiri dari K (pemberian akuades), KN (pemaparan pestisida paraquat), P1 (pemaparan pestisida paraquat dan pemberian ekstrak bandotan 200 mg/kg BB), P2 (pemaparan pestisida paraquat dan pemberian ekstrak bandotan 400 mg/kg BB). Pemberian ekstrak dan paparan paraquat dilakukan selama satu minggu, dimana pemberian ekstrak diberikan satu jam setelah diberi paparan paraquat. Pada hari ke-7 tikus diambil sampel darahnya untuk dilakukan pengujian SGOT dan SGPT kemudian dieutanasi dan dibedah untuk diambil hati dan ginjalnya. Organ dipreparasi menggunakan metode parafin dan pewarnaan hematoksilin-eosin. Parameter pengamatan terdiri dari rasio index hati, bobot hati, luas area sel hepatosit, luas area inflamasi, sel hepatosit nekrosis, SGOT, dan SGPT. Seluruh parameter data dianalisis menggunakan uji ANOVA dan uji lanjutan Duncan (p<0,05). Hasil penelitian menunjukan bahwa perbaikan paling signifikan pada rasio index hati, bobot hati, luas area sel hepatosit, luas area inflamasi, sel hepatosit nekrosis, SGOT, dan SGPT yaitu pada perlakuan dosis 400 mg/Kg BB (p<0,05). Kesimpulan menunjukan bahwa pemberian dosis 400 mg/kg BB ekstrak etanol bandotan dapat melindungi hati serta menjaga kadar SGOT dan SGPT Tikus Wistar yang dipapar paraquat dicloride.
GELLAN GUM, A SPHINGOMONAS MICROBIAL POLYSACCHARIDES: BIBLIOMETRIC ANALYSIS OF TRENDS AND APPLICATIONS Fahmi Achmad Saputra; Indria Puti Mustika; Tiolita, Fitriana; Rudiyono
Berita Biologi Vol 23 No 2 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.5619

Abstract

Bacterial polysaccharides have gained considerable interest in recent years because of their wide range of applications. Gellan gum (GG) is a biodegradable microbial exopolysaccharide that has a wide range of commercial applications due to its versatility. Although GG is being used more frequently, research on it continues to evolve. This study used bibliometric analysis to examine patterns in GG application research over the last four decades. The data were obtained from the Scopus database, and the study of performance was carried out using R-Studio Biblioshiny. The important factors that were evaluated included the type of documents, the annual trends in publication, the subject areas covered, the titles of the journals, the analysis of citations, the distribution of countries, and the analysis of keywords. A total of 714 documents associated to GG were found, with articles being the most common document type. There has been a significant increase in research on GG applications since 2009. China became the primary provider, but the United States showed substantial participation in international collaborations. VOSviewer was used to show the co-authorship between countries and the co-occurrence of keywords in the field of GG research, emphasizing the predominant concentration on biomedical topics. The findings suggest that emergent research trends are primed for additional industrial growth.
BIODEGRADASI PLASTIK OLEH CAMPURAN BAKTERI LIPOLITIK DI DALAM KOLOM WINOGRADSKY Rahayu, Triastuti; Sidiq, Yasir; Harismah, Kun; Santhyami, Santhyami; Agustina, Putri; Haryanto, Haryanto; Tyastuti, Erma Musbita; Aurelia, Fanisha; Sabdina, Berlian Achya Putri
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.5706

Abstract

Plastik menjadi masalah serius karena sangat lambat didegradasi. Oleh karena itu banyak usaha dilakukan untuk mempercepat degradasinya tetapi belum menunjukkan hasil yang signifikan. Salah satu upaya yang dicoba adalah menggunakan bakteri lipolitik. Tujuan penelitian ini adalah menguji kemampuan degradasi plastik menggunakan campuran bakteri lipolitik dalam kolom Winogradsky. Penelitian ini merupakan penelitian eksperimen dengan 2 faktor perlakuan. Faktor 1; jenis plastik (P1/kresek hitam putih) dan (P2 /bungkus mie instan). Faktor 2; waktu inkubasi (W1/1,5 bulan) dan (W2/3 bulan). Plastik diuji degradasi menggunakan campuran bakteri B9 dan B28 dalam kolom Winogradsky. Data yang dianalisis berupa uji antagonisme dan identifikasi bakteri, persen pengurangan berat kering, SEM, dan FTIR. Kedua isolat tidak menunjukkan antagonisme. Isolat B9 teridentifikasi Staphylococcus saprophyticus, sedangkan B28 adalah Bacillus amyloliquifaciens. Persen pengurangan berat kering P1 lebih besar dibanding P2 sebesar 5,11 (4,91%) dan 4,99 (3,99%) masing-masing untuk 1,5 dan 3 bulan. Permukaan P2 terbentuk kerutan lebih banyak dan terdapat cekungan yang dalam di beberapa titik, sedangkan P1 masih tampak lebih halus. Hasil analisis FTIR P2 menunjukkan  munculnya spektra senyawa amina, alkane dan CO2. Campuran bakteri Staphylococcus saprophyticus dan Bacillus amyloliquifaciens mempunyai kemampuan mendegradasi plastik dalam kolom Winogradsky dan plastik 2 menunjukkan tingkat degradasi lebih lanjut dibandingkan plastik 1.
AKTIVITAS ANTIBAKTERI EKSTRAK DAUN BANDOTAN (Ageratum conyzoides) TERHADAP Bacillus cereus DAN Salmonella typhi Hartoyo, Vania Marsela; Sidharta, Boy; Widiastuti, Stefani Santi
Berita Biologi Vol 24 No 1 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.5760

Abstract

Diare merupakan penyakit dengan frekuensi buang air besar yang sering dan feses menjadi cair. Salah satu bahan alam yang dapat digunakan untuk mengatasi diare yaitu bandotan(Ageratum conyzoides). Penelitian ini bertujuan untuk melihat kemampuan ekstrak etanol daun bandotan dalam menghambat Bacillus cereus dan Salmonella typhi. Metode ekstraksi yang digunakan yaitu maserasi menggunakan pelarut etanol 96%. Hasil analisis fitokimia kualitatif ekstrak etanol daun bandotan mengandung senyawa alkaloid, flavonoid, tanin, steroid, dan saponin, sedangkan analisis kuantitatif flavonoid ekstrak etanol daun bandotan didapatkan nilai TFC 36,07mgQE/g ekstrak. Aktivitas antibakteri dilakukan dengan variasi konsentrasi ekstrak yaitu 10, 35, dan 60%. Hasil pengukuran luas zona hambat (LZH) dianalisis dengan ANOVA dan dilanjutkan dengan Duncan Multiple Range Test (DMRT). Konsentrasi ekstrak 60% menunjukkan LZH paling besar dibandingkan konsentrasi ekstrak lainnya yang menghambat pertumbuhan B. cereus dan S. typhi yaitu dengan rata-rata LZH masing-masing yaitu 8,79 cm2 dan 7,61 cm2. Konsentrasi ekstrak 10% menghambat pertumbuhan B. cereus dan S. typhi dengan LZH masing-masing 0,32 cm2 dan 0,30 cm2, sedangkan kontrol negatif 0 cm2 dan 0 cm2. Hasil uji Konsentrasi Hambat Minimum (KHM) ekstrak etanol daun bandotan terhadap B. cereus dan S. typhi didapatkan pada konsentrasi 15%.
ASSESMENT OF THE DNA BARCODES CHARACTERISTIC AND EVALUATION OF PHYLOGENETIC RELATIONSHIP OF Castanopsis argentea (Blume) A. DC. Chika, Syifara; Zahro, Shofiyyatuz
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.5800

Abstract

Castanopsis, the third largest genus under Fagaceae, is widespread in tropical and subtropical areas in East and South Asia. This plant is one of the woody plants that has the potential to be developed because it is helpful for wildlife for nesting and is used in land reforestation activities. Based on data from the Red List of the International Union for the Conservation of Nature and Natural Resources (IUCN), it is reported that the Castanopsis argentea species is threatened with extinction. Research based on genetic aspects of this species is also rarely carried out. This research aims to conduct an in silico study and analyze the kinship relationships of Castanopsis argentea. The method used in this research is the in silico method, which takes Castanopsis argentea nucleotide data from NCBI in the matK region. Based on this research, the phylogenetic results show that the results of the phylogenetic tree reconstruction show that the matK region is divided into two ingroup clades and one outgroup. The matK region in Castanopsis argentea is highly conserved because it only has three genetic variations namely N282T, C285T, and C422A. In this study, the matK gene can group species from the same genus and separate species from different genus. This is in line with the results of the phylogenetic tree, which shows that members of the same genus successfully grouped into one clade. More research on DNA barcoding of Castanopsis argentea must be carried out and developed because more genetic data still needs to be collected at NCBI. The genetic data of a species is essential to research and is stored in NCBI's Genbank for genetic conservation purposes.
STUDY OF TB STATUS IN INDONESIA AND ENDOPHYTIC ACTINOMYCETES AS POTENTIAL SOURCE OF NATURAL PRODUCT FOR ANTI-TUBERCULOSIS DRUG RESISTANCE Ewaldo, Muhammad Farrel; Rukmana, Andriansjah; Nurkanto, Arif
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.6267

Abstract

Tuberculosis, caused by Mycobacterium tuberculosis, is a significant contributor to global mortality, being responsible for one of the primary causes of death worldwide. The current problem is antibiotic resistance in the treatment of tuberculosis patients, which causes the decreasing effectiveness of drugs for tuberculosis therapy. Therefore, exploration for finding new drugs is still necessary. Actinomycetes represent a group of microbes known for producing bioactive compounds, particularly in the form of antibiotics. This microbe can be found associated with plants as endophytic actinomycetes may have the same or similar secondary metabolites as their host. In certain instances, metabolites generated by endophytic actinomycetes exhibit uniqueness and hold the potential to combat antibiotic-resistant pathogenic microbes effectively. This review focuses on new ideas for exploring the potential of endophytic actinomycetes from medicinal plants, especially in Indonesia, considering existing tuberculosis conditions using reviews from scientific sources such as scientific journals, case reports, official government websites, and tuberculosis drug development. This review was then compiled and created a new idea to explore endophytic actinomycetes from medicinal plants in Indonesia using validated methods and contemporary research.
BIOACTIVITY OF ENDOPHYTIC FUNGI EXTRACT ISOLATED FROM THE LEAVES OF MISTLETOE (Dendrophthoe pentandra (L.) Miq.) ON THE LIME PLANT (Citrus aurantifolia) Hiras Habisukan, Ummi; Oktiansyah, Rian; Noviyanto; Anjeli, Riri
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.7423

Abstract

Mistletoe (Dendrophthoe pentandra (L.) Miq.) is a parasitic plant used in traditional medicine. This study evaluates the bioactivity of endophytic fungi in mistletoe . Endophytic fungal species were determined through morphological identification. Potato Dextrose Broth (PDB) media was used for the cultivation, and ethyl acetate was used as solvent to extract secondary metabolites. The antioxidant test was carried out using the DPPH method, while the paper disc diffusion method performed the antibacterial test. A total of 4 isolates of endophytic fungi were obtained from mistletoe leaves, namely isolates DB1 – DB4. The results of morphological analysis showed that DB1 was Paecilomyces sp., DB2 was Papulaspora sp., DB3 was Aspergillus sp., and DB4 was Mucor sp. The endophytic fungus DB3 (Aspergillus sp.) showed the most potential antioxidant and antibacterial activity. This endophytic fungal extract can potentially be a source of new drugs through further research by isolating the active compound.  
A CONTROL OF OXYGEN SUPPLY IN BIOCONVERSION OF SUGARCANE TRASH INTO XYLITOL BY Meyerozyma guilliermondii InaCCY65 Thontowi, Ahmad; Kanti, Atit; Listiyowati, Sri; Meryandini, Anja
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.7720

Abstract

Xylitol is a sugar alcohol used as a sugar substitute for several prevention of health cases such as dental diseases, diabetes, and other health problems. Bioconversion of xylose into xylitol needs an optimum oxygen supply for xylitol synthesis. This research aims to determine the effect of dissolved oxygen on hydrolysate fermentation from sugar cane leaf as a source of xylose to xylitol by Meyerozyma guilliermondii InaCCY65. Dissolved oxygen was varied in aeration and fermentation agitation using a 3L scale bioreactor. Analysis of cell growth for several kinetic parameters during fermentation, xylose reductase, and xylitol dehydrogenase activity. Bioconversion of sugarcane trash hydrolysate into xylitol by M. guilliermondii InaCCY65 is influenced by the oxygen transfer coefficient (kLa) and aeration conditions. The increase in kLa number showed increased cell growth, xylose consumption, xylitol production, and decreased InaCCY65 cells. The optimum conditions of kLa were obtained at 45/h with 39 g/L xylitol production under the aeration effect. Optimum aeration in the bioconversion of sugarcane trash (SCT) hydrolysate become xylitol by M. guilliermondii InaCCY65 is 1.0%; under these conditions, xylitol yield and xylitol productivity are 0.78 g/g and 1.20 g/Lh. The effect of oxygen in the bioconversion of xylose to xylitol also has an impact on xylose reductase (XR) and xylose dehydrogenase (XDH) activities of M. guiellermondii InaCCY65. The results showed that the dissolved oxygen concentration must be carefully controlled during xylitol bioconversion to obtain efficient xylitol.
SPECIES COMPOSITION OF INTERTIDAL BIVALVES IN PRENDUAN BEACH, MADURA ISLAND Nazihah, Fathimah Qonita; Ambarwati, Reni; Isnaningsih, Nur Rohmatin
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.7831

Abstract

Prenduan Beach on the southern coast of Sumenep Regency, Madura Island, is a habitat for various species of bivalves that may be used further. The objective of our research is to document and examine the bivalvia population in Prenduan Beach. Sampling was carried out using the 5 x 5 m quadrat plot method with a 1 x 1 m subplot at three stations with different substrates. The species diversity index (Shannon-Wiener) ranged from 1.15 to 1.36 and was classified as low. Relative abundance (RA) ranged from 0.63 to 60.63%. This study collected 159 bivalvia specimens from seven families and 11 distinct species. The two species with the highest relative abundance were Anadara antiquata (60.68%) and Solen sp. (15.72%). Both species have the potential to be used as a source of seafood.
Cultural Importance Of Useful Plants In Kenduri Sko Traditional Ceremony Of Kerinci Community, Hamparan Rawang Sub-District, Sungai Penuh City, Jambi Province Adriadi, Ade; Asra, Revis; Wahyuni, Fitra; Marsanda, Firda Dwi; Hikmah , Nurul
Berita Biologi Vol 24 No 1 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.8326

Abstract

Hamparan Rawang District is one of the sub-districts in Sungai Penuh City, Jambi Province which has a culture and tradition. One of the traditional ceremonies in Hamparan Rawang District is the Kenduri Sko Traditional Ceremony or what is called Kenduri Pusako. Assessment of the cultural importance of plant species diversity in Kenduri Sko is useful in ethnobotanical studies which include subsistence activity strategies and traditional classifications. Data collection was carried out by interviewing key informants using the "in depth interview" method, then estimating the cultural importance value of each plant species used in Kenduri Sko with the ICS(Index Cultural Significance) formula. The results of the study found that Kenduri Sko is a tradition that symbolises the greatness of customs in their respective regions with the aim of improving friendship and which is characterised by the crowning of traditional titles, cleaning heirlooms and uniting male children of butino children. Based on the results of interviews and estimation of ICS values, it is known that the ICS value of useful plants for Kenduri Sko ranges from 8 to 50. Areca nut (Areca catechu L ) has the highest ICS value, while Cynodon dactylon (L.) Pers. has the lowest ICS value.

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