cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kab. sleman,
Daerah istimewa yogyakarta
INDONESIA
Biology, Medicine, & Natural Product Chemistry
Published by Universitas Islam Negeri Sunan Kalijaga Yogyakarta
ISSN : -     EISSN : -     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health
BIOLOGY, MEDICINE, & NATURAL PRODUCT CHEMISTRY, this journal is published to attract and disseminate innovative and expert findings in the fields of plant, animal, and microorganism secondary metabolite, and also the effect of natural product on biological system as a reference source for researchers in these fields, and with the aim to set international standards in their methodology.
Arjuna Subject : -
Articles 705 Documents
 67   68   69   70   71 
Phytochemical Screening and Antioxidant Studies of Trigonella foenum-graecum Seed Extract Abdulgafar Omeiza Zubair; Patience Anazuo Evinemi; Olurotimi Olutimehin Demokun; Rofiat Oyine Muhammed-Abdulgafar
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1737-1741

Abstract

Drugs are available to combact free radicals and other health related diseases. Fenugreek is one of the medicinal plants to check radicals and other health related diseases. The bioactive compounds in Trigonella foenum-graecum seed were extracted using Methanol through cold merceration technique. The phytochemical screening and antioxidant activities were carried out on the seed using standard methods. The results of phytochemical analysis carried out on the Methanol seed extract of Fenugreek indicates flavonoid, phenol, terpenoid, alkaloids, tannin, Glycoside, Fatty acid, phenols and saponins. However, the IC50 details was evaluated using DPPH free radical. The result of antioxidant activity of the ethylacetate extract exhibited high inhibition at low concentration of 0.1520 mg/cm3 of DPPH while low inhibition of chloroform extract at high concentration of 0.4816 mg/cm3.
Regenerative Hematinic Properties of Mondia whitei Root Aqueous Extract in Phenylhydrazine Hydrochloride-Induced Anemia in Female Wistar Rats MacDonald Idu; Benjamin Ogunma Gabriel; Aye-Mola Glory Dudun; Rametu Momodu
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1691-1698

Abstract

Anaemia, a medical condition worldwide, is of significant concern as it lowers hemoglobin or red blood cell levels, causing potential risk or death to patients. This study investigates the hematinic properties of Mondia whitei on phenylhydrazine hydrochloride-induced anaemic rats. Freshly prepared root of M. whitei was chopped into pieces, washed, shade-dried, and processed into aqueous extract. Phenylhydrazine hydrochloride (40 mg/kg) was administered across the groups for 3 days to induce anaemia in female Wistar rats. M. whitei extract (25, 50,100 mg/kg) and reference drug (folate 5 mg/kg) were orally administered for 7 days across the treatment groups, and hematological, peripheral blood smear, and histopathology parameters were analyzed. The results obtained from this study showed that the root extract exhibited a significant difference in the hematinic properties (red blood cells and their components, peripheral blood smear, and regeneration and rapid maturation of blood cells with normal blood morphology) across graded doses of the extract when compared with the untreated control p>0.05. The plant's traditional use in managing anaemia was scientifically validated, hence further studies are warranted to elucidate the implicated compound-to-compound isolation-guided bioassay.
Malondialdehyde Levels in Wistar Rat Liver after High Fat Diet and Mango Tea Mistletoe Extract Intake Putri Maharani Aulia Rakhman; Ari Hayati; Nour Athiroh Abdoes Sjakoer
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1649-1653

Abstract

Malondialdehyde (MDA) levels increase due to the accumulation of free radicals that exceed normal limits, leading to oxidative stress. Mango mistletoe and tea mistletoe (MESA-DP) are known to contain secondary metabolites that function as natural antioxidants. The use of antioxidants derived from these mistletoes can serve as an alternative to reduce MDA levels and neutralize free radicals. This study aimed to determine the dose of MESA-DP extract that is more effective in reducing MDA levels. This research employed a Randomized Pre Test–Post Test Control Group design using 35 male Wistar rats (Rattus norvegicus), which were divided into five treatment groups with 4 repetition: G1 normotensive as a control without high fat diet (HFD) and without gavage; G2 as an HFD control without gavage; G3 receiving HFD and MESA-DP of 50 mg/KgBW; G4 receiving HFD and MESA-DP of 100 mg/KgBW; and G5 receiving HFD and MESA-DP of 200 mg/KgBW. Data were analyzed using a one-way analysis of variance (ANOVA) to determine differences in hepatic MDA levels among groups. The results showed significant differences in hepatic MDA. Levels among all treatment groups following administration of MESA-DP extracts. The extracts demonstrated an effect in reducing MDA levels and enhance SOD levels thanks to its antioxidant properties. The most effective doses were found in group G3 50 mg/KgBW with an MDA level of 17,604 ± 0,389.
Activity of Antimycobacterial Candidate from Bacteriocins and Lactic Acid Isolated from Lactobacillus bulgaricus Against Mycobacterium tuberculosis Prima Nanda Fauziah; Imas Latifah; Ratna Mutu Manikam; Masdianto Masdianto
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1605-1610

Abstract

Mycobacterium tuberculosis (Mtb) is a bacterium that causes a respiratory infectious disease known as tuberculosis. Tuberculosis (TB) is a global infectious disease that causes death. The current TB treatment method has a weakness, namely the tendency of patients to disobey the rules in taking drugs because they have to take several tablets of medicine for a long time. As a result, some strains of M.tuberculosis can strengthen themselves and become resistant to antimycobacterial drugs used during therapy. Based on these tuberculosis control problems, the WHO declared a global TB emergency.  The low effectiveness of currently used antitubercular drugs encourages the development of new antimycobacterial drugs with low side effects, fast-acting, broadly targeted, and can damage the virulence properties of M.tuberculosis so that the immune system can phagocytose M.tuberculosis. A recent solution from some researchers is to induce immunomodulatory effects that help during the tuberculosis infection process, including from lactic acid bacteria (LAB). This encourages researchers to reveal the interaction of probiotic L.bulgaricus on reducing the growth of M.tuberculosis by finding the main substance with antitubercular activity to obtain optimal results. This study used sensitive M.tuberculosis strain H37Rv to get complete preliminary data. This study tested lactic acid and bacteriocin of Lactobacillus bulgaricus at concentrations of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100%. M.tuberculosis bacteria were inoculated on media containing lactic acid and L. bulgaricus ATCC 11842 bacteriocin, then incubated at 37°C and observed every week from the fourth to the eighth week. Of the five concentrations of lactic acid and bacteriocin tested, only the 60-100% concentration could inhibit the growth of M.tuberculosis. In conclusion, lactic acid and bacteriocin of L. bulgaricus can hinder the growth of M.tuberculosis and can be developed into antimycobacterial drug candidates.
Isolation, Characterization and Antibacterial Studies of Trigonella foenum-graecum Seed Extract Abdulgafar Omeiza Zubair; Sunday Olawale Okeniyi; Rofiat Oyine Muhammed
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1743-1759

Abstract

Pharmaceuticals are available in managing bacteria and other health related diseases. This research focuses on the extraction using Methanol, metabolite screening, fractionation, antibacterial activities, isolation and characterization of the most potent fraction. The phytochemical screening showed Flavonoids, Alkaloids, Tannin, Glycoside, Phenol, Fatty acid, Terpenoids and saponins. The antibacterial sensitivity test carried out on the Hexane fraction using Agar well method showed inhibition range from 0-18 mm (concentration of 12.50-100 mg/cm3) for Staphylococcus aureus, 12-20 mm (concentration of 12.50-100 mg/cm3) for Escherichia coli, 13-22.50 mm (concentration of 12.50-100 mg/cm3) for Salmonella typhi and 0-12 mm (concentration of 12.50-100 mg/cm3) for Bacillus subtillis with an average inhibition range of 0-22.50 mm. For the Chloroform fraction, the antibacterial sensitivity test carried out showed zone of inhibition range from 0-16 mm (concentration of 12.50-100 mg/cm3) for Staphylococcus aureus, 0-12 mm (concentration of 12.50-100 mg/cm3) for Escherichia coli, 0-17 mm (concentration of 12.50-100 mg/cm3) for Salmonella typhi and 0 mm (concentration of 12.50-100 mg/cm3) for Bacillus subtillis with an average inhibition range of 0-17 mm. The antibacterial sensitivity test carried out on the Ethyl acetate fraction showed zone of inhibition range of 0 mm (concentration of 12.50-100 mg/cm3) for Staphylococcus aureus, 0-13 mm (concentration of 12.50-100 mg/cm3) for Escherichia coli, 0-12 mm (concentration of 12.50-100 mg/cm3) for Salmonella typhi and 0 mm (concentration of 12.50-100 mg/cm3) for Bacillus subtillis with an average inhibition range of 0-13 mm. From the above results, the n-Hexane fraction was subjected to the glass column separation technique and an ester of Methylhexadecanoic acid was gotten from the n-hexane fraction of Methanol extract of the seed of Trigonella foenum-graecum.
Anti-inflammatory and Nociceptive Properties of the Root Aqueous Extract of Vernonia amygdalina and Ocimum graticimum Using Animal Models Benjamin Ogunma Gabriel; Marian Jegbefumere Osemwegie; Innocent Inomo Inomo; Ofunre Ozolua Eboreime
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1699-1704

Abstract

Vernonia amygdalina is a medicinal plant used in folk medicine for the treatment of several diseases. Ocimum graticimum plant roots act as sedatives for children. Ocimum tea is an infused form of the herb used in treating fever and diaphoresis. The study aims to evaluate the anti-inflammatory and analgesic properties of the root extracts of biherbal using Swiss mice and Wistar rats. The plant samples were collected, air-dried, ground, and processed into a root biherbal extract. Adult Swiss mice and Wistar rats were obtained to assess anti-inflammatory and analgesic effects using animal models. The results obtained showed a significant reduction in edema at 100 and 200 mg/kg of the extract. The analgesic effects at 100 and 200 mg/kg had a significant reduction in acetic acid and formalin-induced pain models. All effects were statistically significant (p < 0.05). The biherbal extract exhibits significant anti-inflammatory and analgesic effects, with further research needed to identify bioactive compounds and explore its modern medical potential.
Fractionation and Antibacterial Activity of the Methanol Extract of White Champaka Flowers (Magnolia alba (DC.) Figlar) Ulil Amna; Irma Setia Fitri; Halimatussakdiah Halimatussakdiah; Mastura Mastura; Syarifah Yanti Astryna
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1655-1666

Abstract

Research on fractionation and antibacterial activity test of methanol extract of cempaka putih (Magnolia alba (DC.) Figlar) flower was conducted. This study aimed to investigate the class of chemical compounds contained in the methanol extract of cempaka putih (M. alba (DC.) Figlar) flowers and to test the antibacterial activity of the methanol extract of M. alba (DC.) Figlar flowers against Staphylococcus aureus and Escherichia coli bacteria. Based on the results of phytochemical screening, these showed that the methanol extract and its fractions contained secondary metabolites of alkaloid, terpenoids, steroids, saponins, flavonoids, phenols and tannins. Testing for antibacterial activity was carried out by the Kirby-Bauer method (agar diffusion) against S. aureus and E. coli bacteria, at concentrations of 5, 10, 20 and 40 ppm. The antibacterial activity test showed that the methanol extract gave moderate activity against E. coli and fraction A gave moderate activity against S. aureus. While other fractions showed weak activities against both bacteria.
The Effect of Liquid Organic Fertilizer Derived from Mustard Green Vegetable Waste on the Growth of Cayenne Pepper Plants (Capsicum frutescens) Firna Amanda; Manap Trianto; Lestari M.P Alibasyah; Aan Febriawan; Amalia Buntu; Ika Istadewi; Lilies Tangge
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1611-1620

Abstract

Organic fertilizer is a type of fertilizer produced from natural materials derived from living organisms. One form of organic fertilizer is liquid organic fertilizer, which has the advantage of providing nutrients that are more readily available and easily absorbed by plants. This study aimed to analyze the effect of different concentrations of liquid organic fertilizer derived from mustard green vegetable waste on the growth of cayenne pepper plants (Capsicum frutescens L.). The study employed a Completely Randomized Design (CRD) consisting of five treatment levels: P0 (control), P1 5% (10 mL), P2 10% (20 mL), P3 15% (30 mL), and P4 20% (40 mL). Each treatment was replicated three times, resulting in a total of 15 experimental units. The observed parameters included plant height, stem diameter, and number of leaves. Data were analyzed statistically using Analysis of Variance (ANOVA), followed by the Least Significant Difference (LSD) test when significant differences were detected. The results showed that the application of liquid organic fertilizer derived from mustard green vegetable waste had a significant effect on plant height, stem diameter, and leaf number of cayenne pepper plants. Treatment P2 with a concentration of 10% (20 mL) produced the best results across all observed parameters. This finding indicates that this concentration was able to provide sufficient and balanced nutrients to support vegetative plant growth, whereas increasing the concentration to 15% and 20% did not result in a significant improvement in growth. Therefore, liquid organic fertilizer derived from mustard green vegetable waste has the potential to be utilized as an alternative organic fertilizer for enhancing the growth of cayenne pepper plants, with an optimum concentration of 10% (20 mL).
Phytochemical Screening of Tamarind (Tamarindus indica L.) Seed and Peel Extracts under a Simplex-Centroid Solvent Design: Implications for the Valorization of Agro-Industrial By-Products Fabrício Moreira Marques; Bruno Fernando de Souza; Rosimere Campos Ferreira; Michele Cristina Vieira; Maurício Soares Barbosa; Cintia Lacerda Ramos; Harriman Aley Morais
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1705-1712

Abstract

This study aimed to evaluate the phytochemical profile of tamarind seed and peel extracts using a simplex-centroid solvent design. Qualitative screening revealed a broader diversity of metabolites in seed extracts compared to peels. The solvent system showed limited influence on qualitative detection, indicating that matrix composition plays a dominant role. These findings highlight the potential of tamarind seeds as a source of bioactive compounds and support their valorization in functional food and bioproduct applications. However, further quantitative and biological studies are required. Tamarind (Tamarindus indica L.) processing generates significant amounts of seeds and peels, which are often underutilized despite their potential as sources of bioactive compounds. This study aimed to evaluate the phytochemical profile of tamarind seed and peel extracts using a simplex-centroid solvent design. Qualitative phytochemical screening revealed a broader diversity of metabolites in seed extracts, including flavonoids, tannins, terpenoids, saponins, amino acids, and cardiac glycosides, whereas peel extracts exhibited a limited profile, mainly characterized by reducing sugars. The solvent system showed minimal influence on qualitative detection, indicating that matrix composition plays a dominant role over extraction conditions in this context. The results highlight the relevance of tamarind seeds as a promising matrix for the recovery of bioactive compounds and support their potential valorization within sustainable and circular economy approaches. However, due to the qualitative nature of the analyses, further quantitative and biological studies are required to confirm these findings.
Inhibition of NF-kB Signaling by Extract of Male Papaya Flowers (Carica papaya L.) in Chronic Inflammatory Disease Models Lisa Savitri; Kharisul Ihsan; Elfred Rinaldo Kasimo
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1667-1676

Abstract

Chronic inflammation is closely associated with persistent activation of Nuclear Factor-kappa B (NF-kB) signaling pathways, which regulate the expression of pro-inflammatory cytokines and inflammatory mediators involved in various chronic inflammatory diseases. The present study investigated the anti-inflammatory activity of male papaya flower (C. papaya L.) extract through inhibition of NF-?B signaling in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Male papaya flowers were extracted using 70% ethanol and subjected to phytochemical screening, total phenolic content (TPC), and total flavonoid content (TFC) analyses. Cytotoxicity was evaluated using the MTT assay, while anti-inflammatory activity was assessed through nitric oxide (NO) production, enzyme-linked immunosorbent assay (ELISA) for pro-inflammatory cytokines, western blot analysis, immunofluorescence staining, and DPPH radical scavenging assay. Phytochemical analysis revealed the presence of flavonoids, phenolics, alkaloids, tannins, saponins, and terpenoids. The extract demonstrated high TPC and TFC values of 186.42 ± 5.31 mg GAE/g extract and 97.25 ± 3.84 mg QE/g extract, respectively. Male papaya flower extract exhibited low cytotoxicity at concentrations below 200 µg/mL and significantly suppressed nitric oxide production, TNF-alpha, IL-1beta, and IL-6 secretion in LPS-induced macrophages (p < 0.05). Western blot analysis showed that the extract inhibited phosphorylation of NF-kB p65 and I?B? proteins while reducing COX-2 and iNOS expression. Immunofluorescence analysis further confirmed inhibition of NF-kB nuclear translocation. In addition, the extract demonstrated strong antioxidant activity with an IC50 value of 58.4 µg/mL in the DPPH assay. These findings suggest that male papaya flower extract possesses potent anti-inflammatory and antioxidant properties through suppression of NF-kB signaling pathways and may serve as a promising natural therapeutic candidate for chronic inflammatory diseases.

Page 70 of 71 | Total Record : 705

 67   68   69   70   71 

Filter by Year

2014 2026


Reset
Filter By Issues
All Issue Vol 15, No 1 (2026) Vol 14, No 2 (2025) Vol 14, No 1 (2025) Vol 13, No 2 (2024) Vol 13, No 1 (2024) Vol 12, No 2 (2023) Vol 12, No 1 (2023) Vol 11, No 2 (2022) Vol 11, No 1 (2022) Vol 10, No 2 (2021) Vol 10, No 1 (2021) Vol 9, No 2 (2020) Vol 9, No 1 (2020) Vol 8, No 2 (2019) Vol 8, No 1 (2019) Vol 7, No 2 (2018) Vol 7, No 1 (2018) Vol 6, No 2 (2017) Vol 6, No 1 (2017) Vol 5, No 2 (2016) Vol 5, No 1 (2016) Vol 4, No 2 (2015) Vol 4, No 1 (2015) Vol 3, No 2 (2014) Vol 3, No 1 (2014) More Issue