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INDONESIA
Dentino: Jurnal Kedokteran Gigi
ISSN : 23375310     EISSN : 25274937     DOI : 10.20527
Core Subject : Health,
Dentino [P-ISSN 2337-5310 | E-ISSN 2527-4937] is the journal contains research articles and review of the literature on dentistry which is managed by the Faculty of Dentistry, Lambung Mangkurat University. Dentino published twice a year, every March and September.
Arjuna Subject : -
Articles 530 Documents
THE RELATION BETWEEN DURATION OF FIXED ORTHODONTIC TREATMENT AND ORAL HYGIENE STATUS AMONG STUDENTS OF FACULTY OF DENTISTRY UNIVERSITAS MUSLIM INDONESIA 2017 Yustisia Puspitasari; Aulia Aita Aswar; Ilmianti Ilmianti
Dentino : Jurnal Kedokteran Gigi Vol 3, No 2 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i2.5372

Abstract

Background: Fixed orthodontic treatment is now widely used in society, but people often do not realize the risks of using this appliance such as the rise of oral hygiene and caries problem. The design of this appliance is more difficult to clean than removable orthodontic appliance so it can cause a variety of oral disease if long-term fixed orthodontic treatment is required and the cleanliness of the teeth and mouth is not maintained. According to RISKESDAS 2013, Ministry of Health of Indonesia reported that the prevalence of oral disease reached 25.9%. The results showed an increase in the prevalence of oral disesase compared to RISKESDAS 2007 results. Objective : to investigate the relation between duration of fixed orthodontic treatment and oral hygiene status among the students of Faculty of Dentistry Universitas Muslim Indonesia 2017. Method : The study was conducted as an analytic observational study with cross-sectional study design. Samples of this research were students of Faculty of Dentistry, Universitas Muslim Indonesia, Makassar, Indonesia. Oral hygiene status were analyzed using Attin Index. The result was statiscally analyzed with Chi-Square test. Results : The p value of this research was less than 0.05, therefore the results of this research are statistically significant. Conclusion : Hypothesis was accepted and showed that there was a significant relation between the duration of fixed orthodontic treatment and oral hygiene status among the students of Faculty of Dentistry Universitas Muslim Indonesia 2017.
PERBEDAAN PERUBAHAN WARNA RESIN KOMPOSIT NANOFILLER PADA PERENDAMAN AIR REBUSAN DAUN SIRIH MERAH ( PIPER CROCATUM) DAN OBAT KUMUR NON-ALKOHOL Noor Rizki Aulia; Dewi Puspitasari; Muhammad Yanuar Ichrom Nahzi
Dentino : Jurnal Kedokteran Gigi Vol 2, No 1 (2017)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v2i1.2600

Abstract

ABSTRACTBackground: One of resin composite type is nanofiller. Composite resin has tendency to have color change. One factor that can cause color change on the composite resin as mouthwash. Purpose: To determine the difference of color change between nanofiller composite resin that were immersed in decoction water of red bettel leaves (Piper crocatum) and non-alcohol mouthwash. Method: This study was a true experimental research design with control group design pre-test and post-test. It used disk form sample with 10mm diameters and 2mm of thickness. Total samples were 24, divided into 3 treatment groups immersed in decoction water of red bettel leaves (Piper crocatum), non-alcohol mouthwash and sterile aquadest within 24 hours in the incubator with temperature of 37C. Samples were examined by using optical spectrometer (OPT 101 type of photo detector) and microvolt digital. The statistical test was using One-way ANOVA and Post Hoc Bonferroni with <0.05 significance. Result: The result of this study showed significant color change on nanofiller composite resin after immersion in decoction water ofred bettel leaves (Piper crocatum) and non-alcohol mouthwash. Mean value of color change of resin composite immersed in aquadest (0,15Mv), non-alcohol mouthwash (0,24 Mv) and decoction water of red bettel leaves (Piper crocatum) (0,34Mv). Conclusion: There were difference of color change between nanofiller composite resin using the decoction water of red bettel leaves (Piper crocatum) and non-alcohol mouthwash. It showed that composite resin immersed in decoction water of red bettel leaves (Piper crocatum) had higher color change.Keywords : Resin composite nanofiller, color change, mouthwash
PERBANDINGAN INDEKS KARIES ANTARA ANAK YANG MENGKONSUMSI SUSU BOTOL DENGAN TANPA BOTOL USIA 2-5 TAHUN Tinjauan Playgroup Islam Terpadu Ukhuwah Banjarmasin Ghaitsa Ghaitsa; widodo widodo; Rosihan Adhani
Dentino : Jurnal Kedokteran Gigi Vol 2, No 2 (2017)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v2i2.4001

Abstract

Background: The prevalence of bottle caries in Indonesia is quite high at 48% is due to the high percentage of children drinking milk bottles which given while sleeping. Consuming milk formula using a bottle can cause caries, because the product of milk contain carbohydrates. Carbohydrate which contact with teeth will be fermented by bacteria become acid. This situation will cause the demineralization process that dissolves the enamel structure. Caries which caused by bottle feeding is known as Nursing Mouth Caries (NMC). Purpose: The purpose of this research is to know the difference of caries index of deciduous teeth between children who consumed milk with and without bottle.Methods: The target of this research is the students of Playgroup Islam Terpadu Ukhuwah Banjarmasin age 2-5 years with total sample of 80 children who were divided in to two groups, 40 children consumed milk with bottle and 40 children consumed milk without bottle. This research uses cross sectional design. def-t index is used as the measuring tool for deciduous teeth with caries.Results: The results showed that the index of caries in children who consumed milk with bottle is 5,3 which included in the high category, while the index of caries in children who consumed milk without bottle is 3,4 which included in the medium category. Statictical analysis of the results obtained value of p = 0,032 (p < 0,05) which indicated significant difference between the index of caries children who consumed milk with and without bottle. Children who consumed milk using bottle causing pool and contact with teeth. This situation will lower the pH of mouth and self cleansing diminished so that the process of demineralization happened. Conclusion: The conclusion is caries index in children who consumed milk with using bottle is higher than without using bottle.
INHIBITION ACTIVITY OF BELIMBING WULUH(Averrhoa bilimbi linn) LEAF EXTRACT TO Streptococcus mutans ON ACRYLIC PLATE Debby Saputera; Irma Zufira; Lia Yulia Budiarti
Dentino : Jurnal Kedokteran Gigi Vol 3, No 1 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i1.4612

Abstract

Background: The leaf of Averrhoa bilimbi contains flavonoids and tannins as antibacterial agent against Streptococcus mutans. The extract of Averrhoa bilimbi leaf can be used as a natural alternative ingredients of denture cleanser. Purpose: The main purpose of this research is to know the different antibacterial activities of Averrhoa bilimbi leaf extract 6.25%, 12.5%, 25%, 50%, and 100% against Streptococcus mutans on acrylic plate. Methods: The method of this research was  true experimental with posttest-only with control group design that consisted of Averrhoa bilimbi leaf extract in 6.25%, 12.5%, 25%, 50%, 100% and chlorhexidine gluconate 0.2%. The antibacterial testing method was using diffusion method. The measurement parameter was the amount of inhibition zone formed in the test medium. The data analysis was using Kruskall-Wallis test which resulted in p=0.000 (p<0.05). The result showed that there were differences in the inhibitory activity between each group. The test then continued with  Mann Whitney test that results in p=0.009 (p<0.05) which showed meaningful differences between each group. Results: The smallest inhibitory zone of 6.25% was 10.08 mm, the biggest inhibitory zone of 100% was 23.07 mm, and the inhibitory zone of chlorhexidine gluconate in 0.2% was 25.05 mm. The lower extract concentration inhibitory zone were smaller than the high extract concentration. There was significant increase in inhibitory activity of Averrhoa bilimbi leaf extracts cause by the flavonoids and tannins content as antibacterial. Conclusion: It can be concluded that there are  differences in the inhibitory activity of Averrhoa bilimbi leaf extract and chlorhexidine gluconate 0.2%.
EFEKTIVITAS ANTIFUNGI EKSTRAK METANOL BATANG PISANG MAULI (Musa acuminata) DAN Chlorhexidine gluconate 0,2% TERHADAP Candida albicans Dita Permatasari; Lia Yulia Budiarti; Maharani Laillyza Apriasari
Dentino : Jurnal Kedokteran Gigi Vol 1, No 1 (2016)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v1i1.409

Abstract

ABSTRACT Background: Mauli banana stem extract has antifungal effectiveness against Candida albicans because it has flavonoid, saponin, and tannin substances. Chlorhexidine gluconate 0,2% is widely used to treat oral candidiasis caused by Candida albicans fungal infection. Chlorhexidine gluconate 0,2% is known to have caused side effects. Purpose: The purpose of this study is to knowing the antifungal effectiveness differences between 25%, 80%, 100% mauli stem methanol extracts and chlorhexidine gluconate 0,2% against Candida albicans. Methods: This study was true experimental with post test-only with control group design consisted of 4 treatment groups. Treatment group I,II,III each was given 25%, 80%, 100% mauli banana stem methanol extract respectively and treatment group IV was given chlorhexidine gluconate 0,2% as positive control group. The repetition of each treatment was 7 times. Antifungal effectiveness was assessed by measuring inhibition zone of Candida albicans growth using diffusion method. Results: This research showed that mean inhibition zone of treatment group I (13 mm), treatment group II (17 mm), treatment group III (19 mm), and treatment group IV (21 mm). One-way Anova test indicated that there was significant difference between 25%, 80%, 100% mauli banana stem methanol extracts and chlorhexidine gluconate 0,2%. Conclusion: Based on the Post-hoc Bonferroni test, it can be concluded that 100% concentration mauli banana stem methanol extract had more prominent antifungal effectiveness than 25% and 80% against Candida albicans but still less effective than chlorhexidine gluconate 0,2%.Keywords: mauli banana stem extract, antifungal effectiveness, Candida albicans, chlorhexidine gluconate 0,2%ABSTRAK Latar Belakang: Ekstrak batang pisang mauli memiliki efektivitas antifungi terhadap Candida albicans karena mengandung senyawa flavonoid, saponin, dan tanin. Chlorhexidine gluconate 0,2% biasanya digunakan untuk mengobati kandidiasis oral yang disebabkan oleh infeksi jamur Candida albicans. Chlorhexidine gluconate 0,2% diketahui dapat menimbulkan efek samping. Tujuan: Tujuan penelitian ini adalah untuk mengetahui perbedaan efektivitas antifungi ekstrak metanol batang pisang mauli konsentrasi 25%, 80%, 100% dan chlorhexidine gluconate 0,2% terhadap Candida albicans. Metode: Penelitian ini merupakan penelitian eksperimental murni dengan post test-only with control group design yang terdiri dari 4 perlakuan. Perlakuan I,II,III masing-masing diberikan ekstrak metanol batang pisang mauli konsentrasi 25%, 80%, 100% dan perlakuan IV diberikan chlorhexidine gluconate 0,2% sebagai kontrol positif. Jumlah pengulangan setiap perlakuan adalah 7 kali. Efektivitas antifungi dinilai dari mengukur zona hambat pertumbuhan Candida albicans dengan metode difusi. Hasil: Penelitian ini menunjukkan bahwa rata-rata zona hambat yang terbentuk pada perlakuan I (13 mm), perlakuan II (17 mm), perlakuan III (19 mm), dan perlakuan IV (21 mm). Uji Oneway Anova menunjukkan bahwa terdapat perbedaan bermakna antara efektivitas antifungi ekstrak methanol batang pisang mauli konsentrasi 25%, 80%, 100% dan chlorhexidine gluconate 0,2%.Kesimpulan: Berdasarkan uji Post-hoc Bonferroni dapat disimpulkan bahwa efektivitas antifungi ekstrak metanol batang pisang mauli konsentrasi 100% lebih besar dibandingkan 25% dan 80%, tetapi masih kurang efektif dengan chlorhexidine gluconate 0,2%.
PENINGKATAN LEBAR LENGKUNG GIGI RAHANG ATAS MELALUI PERAWATAN ORTODONTI MENGGUNAKAN SEKRUP EKSPANSI Studi RSGM Gusti Hasan Aman Banjarmasin Nor Sakinah; Diana Wibowo; Zairin Noor Helmi
Dentino : Jurnal Kedokteran Gigi Vol 1, No 1 (2016)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v1i1.426

Abstract

ABSTRACT Background: A screw expansion can be used to expand the arch of fangs toward the transverse and sagital suture, the anterior and posterior dependent type and the deployment of a screw. One advantage the use of screw is used to drive the teeth but the teeth are also used as the retention of equipment. Purpose: This research aimsed to identify the improvement of maxillary dental arch width through orthodontic treatment using expansion screw in RSGM Gusti Hasan Aman Banjarmasin. Methods: This type of research was experimental with pre and post test group design. The subject of this research consist one group who conducted orthodontic treatment using a screw expansion of a rounded tooth on the upper jaw. The sample was a study model of teeth derived from the clinic patients orthodontic as much as 9 people. Result: The result showed that on sample there was an increase on the width of maxillary dental arch with the average of enhancement after interpremolar activation 5x activation 0,92 mm, 10x activation 1,86 mm and intermolar 5x activation 0,99 mm, 10x activation 1,96 mm. Conclusion: Based on this research can be concluded that there was improvement of maxillary dental arch width through orthodontic treatment using expansion screw. ABSTRAK Latar Belakang: Sekrup ekspansi dapat digunakan untuk mengekspansi lengkung geligi ke arah transversal maupun sagital, anterior maupun posterior tergantung jenis dan penempatan sekrup. Salah satu keuntungan pemakaian sekrup adalah dapat digunakan untuk menggerakkan gigi tetapi gigi tersebut juga digunakan sebagai retensi peranti. Tujuan: Penelitian ini bertujuan untuk mengetahui peningkatan lebar lengkung gigi rahang atas melalui perawatan ortodonti menggunakan sekrup ekspansi di RSGM Gusti Hasan Aman Banjarmasin. Metode: Jenis penelitian ini adalah pre eksperimental dengan pre and post test group design. Subjek penelitian ini terdiri dari satu kelompok yang sedang melakukan perawatan ortodonti menggunakan sekrup ekspansi pada lengkung gigi rahang atas. Sampel penelitian ini adalah model studi gigi yang berasal dari pasien bagian klinik ortodonti berjumlah 9 orang. Hasil: Hasil penelitian menunjukkan bahwa pada sampel terdapat peningkatan lebar lengkung gigi rahang atas dengan rata-rata peningkatan setelah aktivasi interpremolar 5x aktivasi 0,92 mm, 10x aktivasi 1,86 mm dan intermolar 5x aktivasi 0,99 mm, 10x aktivasi 1,96 mm. Kesimpulan: Berdasarkan hasil penelitian yang dilakukan dapat diambil kesimpulan bahwa terdapat peningkatan lebar lengkung gigi rahang atas melalui perawatan ortodonti menggunakan sekrup ekspansi.
PERBANDINGAN SENSITIVITAS LIDAH TERHADAP RASA MANIS DAN PAHIT PADA ORANG MENGINANG DAN TIDAK MENGINANG DI KECAMATAN LOKPAIKAT KABUPATEN TAPIN Sunjaya Tunggala; Nurdiana Dewi; Asnawati Asnawati
Dentino : Jurnal Kedokteran Gigi Vol 1, No 2 (2016)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v1i2.566

Abstract

ABSTRACT  Background:Tongue has taste buds which are consisted of taste receptors. Its sensitivity is influenced by several factors including betel chewing habit. Purpose:This study aimed to identify whether tongue sensitivity of sweet and bitter tastes in betel chewing community was lower compared to non-betel chewing people in Kecamatan Lokpaikat Kabupaten Tapin. Methods:This study was an analytical survey with cross sectional approach. Samples were selected using total sampling method. Total samples were 32 people, classified into 2 groups of 16 women with betel chewing habit and 16 women without betel chewing habit. Both groups were given sucrose solution of 4 different concentrations (0,05; 0,1; 0,2 and 0,4g/mL) to test tongue sensitivity of sweet taste and quinine hydro-chloride solution of 4 different concentrations (0,0004; 0,0009; 0,0024 and 0,006g/mL) to test the bitter taste sensitivity. Result:Results shown provided an average scores of 1.875 ± 0.619 for sweet taste sensitivity and 1.250 ± 1.125 for bitter taste sensitivity in betel chewing community and an average scores of 3.687 ± 0.478 for sweet taste sensitivity and 3.000 ± 0.816 for bitter taste sensitivity in nonbetel chewing people. Mann-Whitney test result of both groups indicated a p-value of (0.000). Conclusion:Based on the results, it could be concluded that tongue sensitivity of sweet and bitter tastes in betel chewing community was lower than non-betel chewing people in Kecamatan Lokpaikat Kabupaten Tapin.  Key Words :tongue sensitivity, tongue,betel chewing  ABSTRAK  Latar Belakang:Lidah memiliki taste buds yang mengandung reseptor rasa. Sensitivitasnya dapat dipengaruhi oleh berbagai macam faktor termasuk kebiasaan menginang.Tujuan:Penelitian ini bertujuan untuk mengetahui apakah sensitivitas lidah terhadap rasa manis dan pahit pada orang menginang lebih rendah daripada orang yang tidak menginang di Kecamatan Lokpaikat Kabupaten Tapin. Metode:Penelitian ini merupakan penelitian survey analitik dengan pendekatan cross sectional. Pengambilan sampel menggunakan teknik total sampling. Sampel berjumlah 32 orang yang dibagi menjadi 2 kelompok yaitu 16 wanita dengan kebiasaan menginang dan 16 wanita tanpa kebiasaan menginang. Kedua kelompok sampel diberi larutan sukrosa dengan 4 konsentrasi berbeda (0,05; 0,1; 0,2 dan 0,4g/mL) untuk menguji sensitivitas rasa manis dan larutan quinine hydro-chloride dengan 4 konsentrasi berbeda (0,0004; 0,0009; 0,0024 dan 0,006g/mL) untuk menguji sensitivitas rasa pahit. Hasil: Hasil penelitian pada orang menginang terhadap rasa manis didapatkan skor rata-rata 1,875±0,619  pada rasa pahit didapatkan skor rata-rata 1,250 ±1,125 dan pada orang  tidak menginang terhadap rasa manis didapatkan skor rata-rata 3,687±0,478 pada rasa pahit didapatkan skor ratarata 3,000 ±0,816. Hasil uji Mann-Whitney pada kedua kelompok menunjukkan nilai p (0,000). Kesimpulan:Berdasarkan hasil penelitian ini dapat disimpukan bahwa sensitivitas lidah terhadap rasa manis dan pahit pada orang menginang lebih rendah daripada orang yang tidak  menginang di Kecamatan Lokpaikat Kabupaten Tapin.  Kata-kata kunci : sensitivitas lidah, lidah, menginang
GEOGRAPHIC TONGUE PREVALENCE AND NUTRITIONAL STATUS AMONG FIRST GRADE SCHOOL CHILDREN AT TANJUNGSARI SUBDISTRICT OF SUMEDANG Nita Septiani; Indah Suasani Wahyuni; Risti Saptarini; Nanan Nur’aeny
Dentino : Jurnal Kedokteran Gigi Vol 4, No 2 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v4i2.7053

Abstract

Background: Geographic tongue (GT) is a state of the tongue surface which is characterized by an atrophic filiform papillary, a rising in surrounding borderline with yellowish white appearance, and presentations in varying locations and shapes. One of the predicting factors of GT is nutritional deficiency. Objective: to determine the prevalence of GT and nutritional status among first grade school children at Tanjungsari Subdistrict of Sumedang. Methods: It was a descriptive research with the implementation of survey method. The sampling was obtained according to inclusion criteria. The research locations comprised of three elementary school representatives in Tanjungsari sub-district, Sumedang. Nutritional status was assessed using Food Frequency Questionaire (FFQ) Results: There were 200 children with a prevalence of geographic tongue in a total of 4.5%. Nutritional status obtained a result of 11.11% student included in the above-normal criteria, 22.22% students included in normal criteria, 11.11% student included in low deficiency criteria, 33.33% students included in moderate deficiency criteria, and 22.22% students included in  high deficiency criteria. Conclusion: The prevalence of geographic tongue among first grade school children at Tanjungsari District of Sumedang is 4.5% with the highest nutritional status in moderate deficiency category.Background: Geographic tongue (GT) is a state of the tongue surface which is characterized by an atrophic filiform papillary, a rising in surrounding borderline with yellowish white appearance, and presentations in varying locations and shapes. One of the predicting factors ofGT is nutritional deficiency. Objective: to determine the prevalence of GT and nutritional status among first grade school childrenat Tanjungsari Subdistrict of Sumedang.Methods:It was a descriptive research with the implementation of survey method. The sampling was obtained according to inclusion criteria.The research locations comprised of three elementary school representatives in Tanjungsari sub-district, Sumedang. Nutritional status was assessedusing Food Frequency Questionaire (FFQ)Results: There were 200 children with a prevalence of geographic tongue in a total of 4.5%. Nutritional status obtained a result of 11.11% student included in the above-normal criteria, 22.22% studentsincluded in normal criteria, 11.11% student included in low deficiency criteria, 33.33% students includedin moderate deficiency criteria, and 22.22% students included in  high deficiency criteria. Conclusion: The prevalence of geographic tongue among first grade school children at Tanjungsari District of Sumedang is 4.5% with the highest nutritional status in moderate deficiency category. Keyword:Children, elementary school, first grade, geographic tongue; nutritional status
BIOACTIVE COMPOUNDS OF RASAMALA (ALTINGIA EXCELSA NORNHA) LEAVES AS C-MYC PROTO ONCOGENE EXPRESSION SUPPRESSOR OF HUMAN TONGUE CANCER CELL IN VITRO Risyandi Anwar; Arlette Setiawan; Supriatno Supriatno; Unang Supratman
Dentino : Jurnal Kedokteran Gigi Vol 3, No 2 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i2.5379

Abstract

Background: Tongue cancer is a common neoplasm found in oral cavity. It is characterized by aggressive cell growth, poor prognosis and being the cause of mortality. Objectives: to discover bioactive compounds of Rasamala leaves which possess an activity to inhibit SP-C1 human tongue cancer cell proliferation by reducing the expression of c-Myc proto oncogene. Methods: This is an experimental laboratory study using SP-C1 human tongue cancer cell. Separation of bioactive compounds from Rasamala leaves ethyl acetate extract was using various chromatography techniques guided by antiproliferative assay. Results: Two compounds were produced consisting of kaempferol (1) and quercetin (2). Compound 1 and 2 were tested to assess antiproliferative activity of kaempferol and quercetin upon SP-C1tongue cancer cell. IC50 values obtained from antiproliferative assay of each compound were 0.72 and 0.70 ug/ml respectively. Data analysis using ANCOVA test attained a significant value of α=0.05 and proceeded for probit analysis. The activity of  compound 1 and 2 was tested on c-Myc proto oncogene and it was acquired that compound 1 and 2 can suppress c-myc proto oncogene expression. Conclusion: Rasamala compounds consist of kaempferol (1) and quercetin (2) which possess an activity as tongue cancer cell proliferation inhibitor by reducing c-myc proto oncogene expression.
THE TOXICITY OF METHANOL EXTRACT OF MAULI BANANA STEM (Musa acuminata) AGAINST BONE MARROW MESENCHYMAL STEM CELL IN VITRO Amy Nindia Carabelly; Suhartono Taat Putra; Ketut Suardita
Dentino : Jurnal Kedokteran Gigi Vol 2, No 1 (2017)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v2i1.2595

Abstract

ABSTRACTThe latest regeneration therapy has developed towards the usage of Mesenchymal stem cells (MSC) including for aggressive periodontitis, but limited amounts of MSC need extra growth factor in cells culture process. Growth factor is quite expensive so an alternative source is needed. Mauli banana stem is a proven antioxidant and has the most bioactive tannin. Methanol extract of mauli banana stem is not toxic towards fibroblast cell BHK 21 with 25% concentrate, but there is no research about the toxicity of methanol extract of mauli banana stem against MSC. Purpose: To analyze the toxicity of methanol extract of mauli banana stem against MSC in vitro. Method and source: True experimental using the posttest only control group design. MSC culture with treatment methanol extract of mauli banana stem with dosage 2,5 mg/ml; 5 mg/ml; 7,5 mg/ml; 10 mg/ml. Treatment Con A5 μg/ml is used for positive control group and is not treated as negative control group. Each group is incubated for 24 hours and 48 hours, then it is given reagent MTT and is read with ELISA reader. Result: Kruskal-Wallis and independent-sample T-test result shows that there is a significant difference between treatment group and control group. Conclusion: Methanol extract of mauli banana stem with dosage 2.5 mg/ml; 5 mg/ml; 7.5 mg/ml; 10 mg/ml is toxic towards MSC in vitro under treatment for 24 hours and 48 hours.Key words : Mauli banana stem, Mesenchymal stem cells, Toxicity