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I G. Made Krisna Erawan
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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia
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INDONESIA
Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
Deteksi Molekuler Gen Penyandi Protein Virb11 pada Brucella abortus Isolat Lokal Asal Pinrang, NTT dan Strain Vaksin Maria Gladis Bupu Maze; Didik Handijatno; Wiwik Tyasningsih; Suwarno Suwarno; Agnes Theresia Soelih Estoepangestie; Rahaju Ernawati
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.177 KB) | DOI: 10.19087/jveteriner.2020.21.4.503

Abstract

Brucellosis in cattle is a disease caused by Brucella abortus due to the reduction in livestock population caused by abortion, stillbirth, weak birth, infertility and sterility. Brucella abortus has several potentialvirulence factors, i.e. virB11 gene that encodes VirB11 protein is an important virulence factor acts as an ATPase for assembling organelles when the bacteria replicate, helping to complete the bacterial cycle and agress to another cells. The aim of this study are to re-identification Brucella abortus and detect virB11 gene as encoding of B. abortus VirB11 protein in local isolates from Pinrang, NTT, strain vaccines S19 and RB51. The isolates Brucella abortus were re-cultured in Brucella agar base and re-identification is followed by microscopic with Gram staining and biochemical tested with urease, citrat, indol and TSIA test. virB11gene was detected with PCR method. The PCR result showed virB11 gene have DNA band 720 bp.virB11 gene are present in local isolates from Pinrang, NTT, strain vaccines S19 and RB51.
Perbandingan Deteksi Titer Antibodi Pascavaksinasi Rabies Berbasis Kolorimetri Menggunakan ELISA Reader dan Kamera Telepon Genggam Koekoeh Santoso; Ulfatin Khoiriyah Herowati; Dordia Anindita Rotinsulu; Sri Murtini; Muhammad Yusuf Ridwan; Denny Widya Hikman; Abdul Zahid; Ardilasunu Wicaksono; Arifin Budiman Nugraha; Usamah Afiff; Agus Wijaya; Ridi Arif; Ronald Tarigan; Edi Sukmawinata
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.315 KB) | DOI: 10.19087/jveteriner.2021.22.1.79

Abstract

Rabies is an infectious disease, zoonotic, caused by virus from the genus Lyssa virus and generally transmitted by the bite of rabid animal, especially rabies infected dog. Rabies is preventable but is always fatal to humans if the central nervous system (CNS) is infected. Vaccination has been used as one of rabies prevention programmed. A total of 83 samples were tested using an Indirect ELISA (Enzyme Linked Immunosorbent Assay) to identify post-vaccination rabies antibody titer. Antibody titres correlated with absorbance values and standard solutions concentrations. Absorbance value can be determined using ELISA reader and mobile phone camera. Absorbance were read at 450 nm and 620 nm as reference using ELISA reader and image from mobile phone camera using image processing software (ImageJ). The aim of this study is to compared between ELISA reader as gold standard and mobile phone camera through validity testing such sensitivity, specificity, and accuracy. There is no significant difference between gold standard and alternative test equipment. The mobile phone camera has sensitivity 98,6%, specificity 88.8 % and accuracy 97,5%. The image processing method using ELISA reader is relatively expensive and difficult to hold in laboratory with minimum funds. Image processing method using a mobile phone camera with ImageJ application is expected to be an alternative tool to read the result of ELISA.
Rumen Fermentation Characteristic and In Vitro Digestibility of King Grass Silage Supplemented with Shredded Coconuts Pulp Ayu Septi Anggraeni; Hendra Herdian; Ahmad Sofyan; Anuraga Jayanegara; Nurrul Suri Aulia
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.086 KB)

Abstract

Shredded coconuts pulps are the potential wastes of coconuts product that can be used as a feed alternative because it contains enough nutrients. In the other side fat content of shredded coconut pulp are high enough. High-fat will bother the fermentation process in the rumen. The fermentation process can improve the quality of the digestibility and nutrient content of shredded coconut pulp. The purpose of this study was to evaluate the effect of silage formulation of shredded coconut pulp on in vitro rumen fermentation characteristics. P2 treatment gave the highest gas production than other treatment. Percentage of acetate, propionate, and butyrate, Acetate to Propionate ratio (A:P), Non glucogenic Ratio (NGR), Ammonia-N (NH3) production of shredded coconut pulp was significantly affected by treatments (P<0.05). Total gas production 48 hours, gas production from soluble fraction (a), insoluble fraction (b), total fraction (a+b), total VFA and biomass microbial rumen of silage gave no significant difference among treatments (P>0.05). While in total gas production 48 hours not significant, when it breakdown each hour show significant results, which means there is an increase in feed degradability. In this study silage formulation using, 8% rice bran, 5.4% of shredded coconut pulp and 2.6% “gaplek” flour treatment has the best degradability on in vitro rumen fermentation characteristics. Keyword: silage, shredded coconut pulp, rumen fermentation, in vitro.
Comparison of Hematological and Biochemical Profiles of Late Pregnant and Non-Pregnant Mares Murat Onur Yazlik; Ezgi Dikmeoglu; Arzu Esen; Ufuk Kaya; Özgenur Kafkas; Burak Baran; Murat Göcen
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.946 KB) | DOI: 10.19087/jveteriner.2021.22.1.1

Abstract

The maternal body must adapt to fetal growth and development during the state of pregnancy for successful foal and mother. Pregnancy status as in other mammals is related to physiology and hematology. This study aimed to compare the hematology profiles and biochemical parameters of late pregnant and non-pregnant mares. Twenty-two mares were included in the study. Mares were divided into two groups according to the pregnancy status. Hematology profile and biochemical parameters were analyzed from blood samples collected from the jugular vein. All data were analyzed using Stata 12/MP4. White blood cell count, lymphocyte, red blood cell count, hemoglobin concentration, hematocrit, monocyte and percentage of monocyte were significantly lower in non-pregnant mares compared to late pregnant mares (P<0.05). No significant differences were noted in values of neutrophil, eosinophil, mean cell volume, mean corpuscular hemoglobin concentration, mean corpuscular hemoglobin, platelet, mean platelet volume, red cell distribution width in both groups (P>0.05). On the other hand, biochemical parameters analysis showed that calcium, total bilirubin, and triglyceride concentrations were greater in late pregnant mares (P<0.05). However, urea, albumin, cholesterol, and magnesium concentrations were similar among groups (P>0.05). In conclusion, the present study showed hematology profile and biochemical parameters differences in late pregnant and non-pregnant mares. These values could be useful in clinical practices to assess the situation of late pregnant mares.
Keanekaragaman Ektoparasit pada Kelelawar Subordo Microchiroptera di Goa Jepang Bukit Plawangan, Sleman, Yogyakarta Soenarwan Hery Poerwanto; Luqman Rasyid Ridhwan; Giyantolin Giyantolin; Dhea Ginawati; Desak Putu Raka Paramitha
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.459 KB) | DOI: 10.19087/jveteriner.2020.21.4.629

Abstract

Ectoparasites in bats can cause a decrease in health and can be a disease vector. Ectoparasites found in bats are generally members of the Insect Class and Arachnida Class. This study was aim to determinethe diversity of ectoparasites in bats of the Microchiroptera suborder in Jepang Cave, Plawangan Hill, Sleman, Yogyakarta and knowing various factors that influence the existence of these ectoparasites in bats. Catching bats is done by mist net, bats are anesthetized and morphometric measurements are carried out. Descriptive methods are used to identify bats and their ectoparasites and analyze the abundance of ectoparasites using prevalence and intensity. Identification of ectoparasites were carriedout in the Animal Systematic Laboratory of the Parasitology Section of the Faculty of Biology, Universitas Gadjah Mada, by using the book of ectoparasites identification. Bat species were ound include Miniopterus schreibersii and Rhinolopus pusillus. The results showed that ectoparasite species were found in the batsof the Microchiroptera Suborder from the Subclass Acarina and the Insect Class. The species of Subclass Acarina found were Periglischrus sp., Spinturnix plecotinus, Blattisocius sp., And Glycyphagus sp. Speciesof the Insect Class found include Megastrebla sp., Stylidia caudata, Basilia sp., and Brachytarsina sp. The prevalence of R. pussilus in Jepang Cave, was 66.67% and it is frequent. The ectoparasite intensity categoryof Periglischrus sp. was 0.72, meanwhile Glycyphagus sp. and Stylidia caudata were 0.06, which is very low.
Ekspresi Transforming Growth Factor-beta dan Growth Differentiation Factor-9 Oosit Domba yang Divitrifikasi Sesudah dan Sebelum Maturasi In Vitro Zakiyatul Faizah; Raden Haryanto Aswin
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.212 KB) | DOI: 10.19087/jveteriner.2021.22.1.109

Abstract

Oocyte vitrification today became a hope to preserve fertility. Its was a major challenge because of oocyte characteristic in every phase. Immature oocytes were more sensitive to osmotic stress and the membrane wes less stable while mature oocyte have spindles that were very susceptible to temperature decrease. The study aim to compare the effect of vitrification before and after in vitro maturation to the expression TGF beta and GDF9. Oocyte of ewes divided into control groups (K0), K1 maturation prior vitrification, K2 vitrification prior maturation. Vitrification begins with washing oocytes in PBS supplemented of 20%serum for 1-2 minutes, followed by equilibration medium PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred to 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing was processed by in the media: 1). PBS + 20% serum + 0.5 M sucrose, 2).PBS + 20% serum + 0.25 M sucrose, and 3).PBS + 20% serum + 0.1 M sucrose. Immunocytochemical stain was performed to evaluate TGF ? and GDF9 expression. Remmele scale index (IRS) was used to read the result. TGF beta expression both in oocyte and cummulus of K0 and K1 was significant statistically difference (p<0.05) compare with K2. GDF9 expression both in oocyte and cummulus of K0 and K1 was significant statistically difference (p<0.05) compare with K2. We concluded that immature oocyte give better expression of TGF â and GDF9 than mature oocyte.
In Vitro Ovine Embryo Culture: Effectiveness of TCM-199 in Combination with 17?-Estradiol Hormone and Co-Culture of FTEC Yon Soepri Ondho; Dela Ayu Lestari; Daud Samsudewa
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.646 KB) | DOI: 10.19087/jveteriner.2020.21.4.558

Abstract

Objective of this research was to determine the effectiveness of various culture mediums on thecleavage to morula in in vitro embryo culture of ovine. A total of 1.064 zygotes (fertilized oocytes) were grown in three kinds of different embryo culture medium, which were Tissue Culture Medium 199 (TCM-199) (T1), TCM-199 + 17â-estradiol (T2) and TCM-199 + co-culture of Fallopian Tube Epithelial Cell (FTEC) (T3). Data were analyzed using a completely randomized design with ANOVA analysis and HonestlySignificant Difference test. The variable observed were the percentage of embryo cleavage and morula. Results showed that T3 had the highest percentage (72.45%) of the cleavage occurred in zygotes cultured in the TCM-199 + FTEC co-culture medium, as well as embryo growth to the morula stage (75.75%). Incontrast, zygotes cultured in T1 and T2 had lower cleavage and lower morula development than T3, as much as 27.38%; 47.82% and 58.15%; 66.66%, respectively. In conclusion, TCM-199 + FTEC co-culture medium is the best medium for embryo culture.
LYMNAEA SPP. SNAIL AS INTERMEDIATE HOST OF TREMATODE IN PRAFI DISTRICT, MANOKWARI, PAPUA BARAT Noviyanti Noviyanti; Angelina Novita Tethool; Purwaningsih Purwaningsih; Fadjar Satrija; Sri Murtini
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.555 KB)

Abstract

Paramphistomum spp. and Fasciola spp. are the most common trematodes that infect cattle throughout the world including in Manokwari, West Papua. During their life cycle, freshwater snails act as the intermediate host for the larval. This study aims to provide information about the presence of cercariae –the larval form of trematode- within the body of Lymnaea spp. snail. The study was conducted in August-September 2019 in Prafi District, Manokwari, West Papua. Lymnaea spp. were collected from 15 permanent and seasonal drainage around a water dam, rice field irrigation, and palm oil plantations. Snails are collected by hand using a quadrant or using the "man per minute" method. Cercaria within the snail's body is examined using the "crushing method" under a light microscope. Snails that have cercaria trematodes are categorized as infected snails. The percentage of snails infected with trematodes in August and September were 15.92% (53 out of 333 snails) and 38.05% (78 out of 205 snails), respectively. Cercariae that were identified were longifurcate-pharyngeate cercariae (Strigea cercaria), Cercariaeum cercaria, and Virgulate cercaria. The adult phase of these cercaria are generally exist in the intestine of mammals, amphibians, birds and fish. Further research is needed to provide more accurate information about the epidemiology and morphology of trematodes in freshwater snails.
Seroprevalensi Virus Avian Influenza H9N2 pada Ayam Kampung (Gallus domesticus) di Pasar Beringkit, Kabupaten Badung, Bali Brigita Galilea Adu; Messy Saputri Boru Sembiring; Oktryna Hodesi Sibarani; I Gusti Ngurah Kade Mahardika; Ida Bagus Kade Suardana; I Gusti Ayu Agung Suartini; Tjokorda Sari Nindhia
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.636 KB)

Abstract

Virus Avian Influenza (Avian Influenza Virus/AIV) subtipe H9N2 (AIV-H9N2) telah menjadi perhatian bagi kesehatan unggas. Virus ini telah dilaporkan di beberapa provinsi di Indonesia. Pasar Beringkit merupakan pasar unggas yang menerima suplai unggas dari berbagai daerah di Bali. Pasar ini menjual berbagai jenis unggas seperti: ayam, itik dan ayam kampung. Penelitian ini bertujuan untuk mengetahui seroprevalensi virus Avian Influenza subtipe H9N2 pada unggas domestik di pasar Beringkit, Kabupaten Badung, Bali. Sebanyak 187 sampel darah dikumpulkan dari tiga kali pengambilan yang berbeda. Serum diambil dari ayam broiler, ayam kampung dan itik yang belum divaksin dan diuji menggunakan Hambatan Hemaglutinasi (Haemagglutination Inhibition/HI). Serum diencerkan lima kali dengan NaCl dan dipanaskan 55oC selama 30 menit sebelum dilakukan pengujian. Hasil pemeriksaan uji HI dianalisis dengan uji statistik Non-parametrik Chi-Square. Hasil penelitian ini menunjukkan bahwa dari 187 sampel serum,43 sampel positif mengandung antibodi AIV-H9N2. Seroprevalensi AIV-H9N2 pada ayam broiler sebesar 15,9% (dari total 63), ayam kampung 35,5% (dari total 62) dan itik sebesar 17,7% (dari total 62). Hasil analisis statistika menunjukkan bahwa sampel yang diambil dari antar spesies dengan tiga kali pengambilan berbeda menunjukkan hasil tidak berbeda nyata. Penelitian ini menunjukkan bahwa unggas domestik di Bali telah terinfeksi AIV-H9N2. Biosekuriti, pengawasan pasar dan vaksinasi efektif dalam mencegah infeksi perlu ditingkatkan. Dampak ekonomi yang disebabkan AIV-H9N2 pada unggas domestik perlu dikaji lebih lanjut.
Menduga Berat Karkas pada Sapi Bali Jantan dan Betina Menggunakan Bobot Hidup Tjokorda Sari Nindhia; Elizabeth Kezi Damayanti; Putu Sampurna
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.12 KB) | DOI: 10.19087/jveteriner.2021.22.1.49

Abstract

Penelitian ini bertujuan untuk menduga bobot karkas pada sapi bali jantan dan betina dengan menggunakan bobot hidupnya. Hal tersebut dapat membantu peternak dan pembeli sapi untuk menduga bobot karkas menggunakan bobot hidup. Penelitian ini menggunakan sampel yaitu sapi bali sebanyak 20 ekor jantan dan 24 ekor betina yang dipotong di Rumah Potong Hewan Mambal. Pengumpulan data pada penelitian ini dilakukan dengan pengukuran langsung pada bobot hidup dan bobot karkas. Data yang diperoleh dianalisis dengan independent t-test kemudian dianalisis dengan regression metode power. Hasil rata-rata pengukuran dari bobot hidup pada sapi bali jantan yaitu 312,55±13,96 kg sedangkan pada sapi bali betina 247,00±6,96 kg serta hasil rata-rata pengukuran dari bobot karkas pada sapi bali jantan yaitu 171,87±9,14 kg sedangkan pada sapi bali betina 115,43±3,33 kg. Nilai koefisien korelasi yang didapatkan untuk sapi bali jantan adalah 0,954 sedangkan untuk sapi bali betina adalah 0,918. Nilai koefisien deteminasi (R2) yang didapatkan untuk sapi bali jantan adalah 0,91 dan untuk sapi bali betina adalah 0,843 sehingga ditemukan hubungan yang erat antara bobot hidup dengan bobot karkas pada sapi bali jantan dan betina. Hasil penelitian ini menunjukkan bobot karkas dapat diduga menggunakan bobot hidup dengan persamaan pada sapi bali jantan yaitu Y= 0,208X1,168 sedangkan pada sapi bali betina yaitu Y=0,763X0,911. Berdasarkan hasil analisis regression metode power dapat diketahui laju perubahan bobot karkas pada sapi bali jantan lebih tinggi daripada sapi bali betina sehingga sapi bali jantan lebih ekonomis untuk dipotong karena memiliki persentase bobot karkas lebih tinggi daripada sapi bali betina.

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