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All Journal Jurnal Ilmu Manajemen Jurnal Teknik Kimia USU Biology, Medicine, & Natural Product Chemistry MPI (Media Pharmaceutica Indonesiana) JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA ALCHEMY Jurnal Penelitian Kimia Jurnal Ilmiah Manajemen "E M O R" JAB (Jurnal Akuntansi & Bisnis) Stability: Journal of Management and Business CARADDE: Jurnal Pengabdian Kepada Masyarakat JPSCR : Journal of Pharmaceutical Science and Clinical Research FITOFARMAKA: Jurnal Ilmiah Farmasi Jurnal Analis Farmasi Oetoesan-Hindia: Telaah Pemikiran Kebangsaan Community Empowerment Advance Sustainable Science, Engineering and Technology (ASSET) Bioscientia Medicina : Journal of Biomedicine and Translational Research JAF (Jurnal Analis Farmasi) Jurnal Ilmu Manajemen SEMINAR NASIONAL PENELITIAN DAN PENGABDIAN KEPADA MASYARAKAT SWAGATI: Journal of Community Service Jurnal Sains dan Kesehatan Advance Sustainable Science, Engineering and Technology (ASSET) Berita Biologi
Marlina, Dian
Universitas Setia Budi
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Comparing Conventional and Modern Methods for The Phycocyanin Extraction from Spirullina sp Dian Marlina; Desi Purwaningsih; Reny Pratiwi; Ryan Werytama Saputra; Widiastuti Setyaningsih; Supriyono Supriyono
Advance Sustainable Science Engineering and Technology Vol. 6 No. 3 (2024): May - July
Publisher : Science and Technology Research Centre Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/asset.v6i3.907

Abstract

Spirulina platensis, a blue-green algae abundant in tropical regions, is rich in minerals, vitamins, fibers, and pigments, with low nucleic acid content. It has unique chromoproteins called phycobiliproteins, notably phycocyanin, used in various applications. This study aims to optimize phycocyanin extraction using different solvents (distilled water and sodium phosphate buffer pH 6.7) and methods (freeze-thaw and sonication). Spirulina platensis biomass was extracted in both solvents, then some of them was freeze for 24 and 48 hours followed by thawing overnight. The other was sonicated for 2.5 minutes, 50 Hz then soaked for 1, 2, and 3 hours. All of the samples were centrifuged at 6000 rpm for 10 minutes and the absorbance was measured using a UV-Vis spectrophotometer at wavelengths of 280, 620, and 650 nm. with freeze-thawing for 48 hours yielded the highest phycocyanin concentration (0.55%), with a yield of 11.07 and purity of 0.21. Sonication improved phycocyanin concentration, yield, and purity significantly, yielding 1.108, 25.85, and 0.26, respectively.
Co-Authors 12.520.20.206 Shinta Ratnawati, 12.520.20.206 Achmad Solechan Amanda, Nathasya Gracya Ana Indrayati Anak Agung Istri Sri Wiadnyani Anggraini, Natasya Dila Putri Aris Tri Jaka Harjanta, Aris Tri Arsyeni Destra Anarchy Arsyandi Asiyah, Siti Dwi Bambang Agus Herlambang, Bambang Desi Purwaningsih Desi Purwaningsih Dwinata Rahayuningsih Emmellia Yunitha Febrian Murti Dewanto Fitrianingsih, Sri Ghani Nurfiana Fadma Sari Gunawan Pamudji Widodo Haryo Kusumo Haryo Kusumo Ira Juliani Anwar Iswandi Iswandi Jason Merari Peranginangin Kusumo, Haryo Lumbantungkup, Gebi Evelin mayang, anissa Mega Novita Mega Novita Misnovita, Ghina Salsabila Muhamad Ansory, Hery Nabilah Nauli Jehan Nilawati, Anita Novitarini Nur Anggreini Dwi Sasangka Nurfiana Rachmawati Pramukantoro, Ganet Eko Prasetyanto, Panji Kusuma Priyanto, Widodo Purwaningsih, Avianti Eka Dewi Aditya Puspitasari, Ismi Putri, Cinthiya Ekwinta Rahmadita, Ivony Reny Pratiwi Rifki Hermana Ryan Werytama Saputra Santi Kartika Dewi, Lensa Erliana Sato, Harumi Senowarsito, Senowarsito Sephia Febriyanti Setiawan, Anang Kuncoro Rachmad Siti Aisiyah Siwi, Andini Prabandaru Supriyono Supriyono Sutomo Sutomo Takad Bagas Aji Prasetyo Titik Sunarni Vivin Nopiyanti Widiastuti Setyaningsih