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All Journal Bioma : Berkala Ilmiah Biologi IPTEK Journal of Proceedings Series Jurnal Kimia Riset Indonesian Journal of Chemical Science Jurnal Surya Medika Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosaintifika: Journal of Biology & Biology Education KLOROFIL: Jurnal Ilmu Biologi dan Terapan Gorontalo Agriculture Technology Journal Jurnal Respirologi Indonesia Jurnal Abdi Insani Spizaetus: Jurnal Biologi dan Pendidikan Biologi SIMBIOSA Humanism : Jurnal Pengabdian Masyarakat KLOROFIL: Jurnal Ilmu Biologi dan Terapan Journal Pharmasci (Journal of Pharmacy and Science) Journal of Experimental and Clinical Pharmacy (JECP) Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Prosiding Seminar Nasional Biologi Jurnal Komunitas Farmasi Nasional IJENSET
Umarudin, Umarudin
Akademi Farmasi Surabaya
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Validation Of Linerity Test Method For Determination Antioxidant Activity of Ascorbic Acid By DPPH Method Using Ethanol Solvent: Validasi Metode Uji Linieritas Pada Penentuan Aktivitas Antioksidan Asam askorbat Dengan Metode DPPH Menggunakan Pelarut Etanol Galuh Gondo Kusumo; Sri Safitri; Mercyska Suryandari; Andhika Dwi Aristyawan; Siti Annurijati Hatidja; umarudin
Journal Pharmasci (Journal of Pharmacy and Science) Vol. 10 No. 1 (2025): Journal Pharmasci (Journal of Pharmacy and Science)
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v10i1.454

Abstract

Method’s validatio is very important in research, including in the antioxidant activity test. Based on USP XXXVII 2014, antioxidant activity test is included in category III research where analytical methods are used to ensure the performance and quality of finished product. Precision is a must in this cathegory and other parameters may be needed. This research aims to test the validation of the linearity of the antioxidant activity method of ascorbic acid using DPPH with ethanol as the antioxidant testing solvent. This study used ascorbic acid samples with 96% p.a. ethanol solvent. The test was conducted by taking 10 mg of ascorbic acid with concentrations of 1, 2, 3, 4, 5 ppm and repeating it 3 times. The data obtained concluded that the absorbance results from replicates 1, 2, and 3 were not significantly different. As the concentration increased, the resulting absorbance decreased. The percentage decrease results from this research can be considered valid because the obtained r values are close to 1, namely replication 1 = 0.9932, replication 2 = 0.9946, replication 3 = 0.9910.
Antibacterial Activity of Vesicularia ferriei Ethanol Extract Againts Staphylococcus aureus: Aktivitas Antibakteri Ekstrak Etanol Lumut Vesicularia ferriei Terhadap Bakteri Staphylococcus aureus Andhika Dwi Aristyawan; Umarudin; Mercyska Suryandari; Silvi Ayu Wulansari; Galuh Gondo Kusumo
Journal Pharmasci (Journal of Pharmacy and Science) Vol. 11 No. 2 (2026): Journal Pharmasci (Journal of Pharmacy and Science)
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v11i2.552

Abstract

Indonesia's tropical climate contributes to a high incidence of bacterial infections, including those caused by Staphylococcus aureus. This Gram-positive bacterium forms irregular clusters, does not produce spores, is facultatively anaerobic, and is immobile. It is responsible for various infections, including skin infections, endocarditis, bacteremia, and pneumonia. Vesicularia ferriei, an aquarium moss commonly used in aquascaping, belongs to the Hypnaceae family and contains secondary metabolites such as flavonoids, flavonols, and triterpenoids. This study aims to determine the antibacterial activity of Vesicularia ferriei ethanol extract against Staphylococcus aureus. The antibacterial activity was tested using the disc diffusion method with the pour plate technique at concentrations of 100 ppm, 500 ppm, 1,000 ppm, 5,000 ppm, and 10,000 ppm, with three replications. The results showed weak inhibition zones around the paper discs at 5,000 ppm (3.76 mm) and 10,000 ppm (4.76 mm). The negative control (10% DMSO) produced no inhibition zone, while the positive control (Cefadroxil antibiotic) exhibited a strong inhibition zone averaging (15.07 mm). These findings indicate that the ethanol extract of Vesicularia ferriei has antibacterial activity against Staphylococcus aureus, albeit in the weak category. This study serves as a reference for further research exploring different extraction methods, antibacterial testing techniques, solvents, and extract concentrations. Additional studies are needed to investigate the broader benefits of Vesicularia ferriei extract and its potential as an antibacterial agent.
Co-Authors Agustin Widia Rani Aisyah Hadi Ramadani Aisyah Hadi Ramadani Anidnya, Widya Dara Anindya, Widya Dara Anisa Rizki Amalia Aristyawan, Andhika Dwi Ayu Wulandari Bawanda, Edward Melfin Beauty Salma Ardiyanti Prameswari Devianti, Vika Ayu Dwi Rahmawati Mufidah Evy Aryanti Fajar Fajar Fredy Kurniawan Galuh Gondo Kusumo Harsini Ina Firdausyiah Jumriah Nur, Jumriah Junairiah Junairiah Lailatus Sa’diyah Madurani, Kartika Anoraga Mercyska Suryandari Mochammad Sulton Aziz Irawan Munifatul Izzati Novicalia Citra Dewi Ramadhan Renaisansa Ramadhan Renaisansa Reviono Reviono Sa’diyah, Lailatus Sa’diyah, Lailatus Selvyronica Eka Agustine Siti Annurijati Hatidja Siti Nurhaliza Sri Safitri Surahmaida, Surahmaida Syafitri, Meyke Herina Syukrianto Syukrianto, Syukrianto Tiffany Wanda Nur Azizah Ulfa, Ninik Mas Wahyu Isma Mayang Iswari Widayati, Yuyun Wulansari, Silvi ayu Yuliarni, Floreta Fiska Yuyun Widayati