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All Journal BIOMA : Jurnal Biologi Makassar Jurnal Kesehatan Komunitas JURNAL FARMASIMED (JFM) Jurnal Ilmiah Kesehatan Rustida Journal of Nursing and Public Health (JNPH) Journal SAGO Gizi dan Kesehatan SEHATI: Jurnal Kesehatan Insight Power (Kekuatan Wawasan) - Jurnal Pengabdian Kepada Masyarakat Digital (JUPED) EJOIN : Jurnal Pengabdian Masyarakat Jurnal Pengmas Kestra (JPK) Jurnal Laboratorium Khatulistiwa Indonesian Journal of Community Empowerment Jurnal Medika: Medika Jurnal Sains, Teknologi dan Kesehatan Journal of Medical Surgical Concerns Medistra Medical Journal (MMJ)

Vincentia Ade Rizky

Universitas Borneo Tarakan

Author-ID : 1239959

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Sosialisasi Efektifitas Penggunaan Pewarna Alternatif Preparat Permanen Telur Nematoda Kolon Menggunakan Pewarna Rhodamin B Saadah Siregar; Vincentia Ade Rizky; Asvia Rahayu; Visensius Krisdianilo
Jurnal Pengabdian Kepada Masyarakat Digital Vol 1, No 3: JUPED - Agustus 2022
Publisher : Insight Power (Kekuatan Wawasan)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.358 KB)

Staining is one way to do research to identify eggworms. The dyeing technique explains the material and description of the worm egg under the microscope and helps to distinguish it from the surrounding feces. A widely used method for staining histological tissue with hematoxylin and eosin. A commonly used stain for the above colon nematode examination is eosin stain. Rhodamine B dye is used instead of eosin dye to add a reference dye. The method used is experimental and uses eosin-controlled rhodamine B concentration ratios of 0.5%, 1%, 1.5%, and 2% to 3%. The worm egg morphology is clearly visible from the outer layer to the inner layer, the field of view of the rhodamine B dye is clearly visible, and the color contrast of the egg is also clearly visible. From the research conducted, it can be concluded that Rhodamine B can be used as an alternative to the dye eosin. After the seminar, participants in the dedication activity realized that dye Rhodamine B was used instead of dye eosin.Keywords: intestinal nematode egg, hematoxylin-eosin, rhodamine B

Perbedaan hasil pemeriksaan jumlah koloni bakteri pada daging ayam broiler dengan pemberian parutan serai (Cymbopogon citratus) setelah 24 jam Saadah Siregar; Vincentia Ade Rizky; Wenda Putri Natal Saragih
Jurnal SAGO Gizi dan Kesehatan Vol 4, No 1 (2022): Juli - Desember
Publisher : Politeknik Kesehatan Kemenkes Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/gikes.v4i1.1073

Background: Chicken meat is one of the most highly nutritious foodstuffs, as it contains nutrients that are indispensable to the human body, such as water, protein, fat, and other organic ingredients. However, the availability of these complete nutrients has caused the meat to become a medium for bacterial growth. Pathogenic bacteria and spoilage bacteria can cause health problems.Objectives: The study aims to determine the difference in examining the number of bacterial colonies in broiler chicken meat by giving lemongrass (Cymbopogon citratus) after 24 hours.Methods: Quantitative research using experimental design through laboratory testing, namely by determining whether or not there is a decrease in the number of germs in broiler chicken meat by giving grated lemongrass leaves after 24 hours. The sample is broiler chicken meat. The research was conducted in 2021. Laboratory testing was carried out using the pour plate method for two repetitions. Each plate of the dilution is calculated as the number of colonies by multiplying the dilution per 1 gram / 1 ml sample. Data were analyzed descriptively and bivariate using Independent T-test at 95% CI.Results: It was found that the growth of total germ numbers in chicken meat without incubation after 24 hours was treatment one was 39,96 x 106 CFU/gram, and treatment two was 39,87 x 106 CFU/gram, while chicken meat incubated for 24 hours was treatment one. The number of colonies in the first treatment was 40,39 x 106 CFU/gram, and 40,00 x 106 CFU/gram in treatment two. While chicken meat with lemongrass grater was incubated after 24 hours, there was a decrease in the number of colonies in the first treatment of 33,91 x 106 CFU/gram and the second treatment of 34,30 x 106 CFU/gram.Conclusion: There was a decrease in the number of germs in broiler chicken meat given grated lemongrass leaves after 24 hours of incubation.

Identifikasi Bakteri Coliform pada Susu Kedelai Menggunakan Metode MPN (Most Probable Number) Vincentia Ade Rizky; Saadah Siregar; Asvia Rahayu; Visensius Krisdianilo; Suci Mustika Mawardani; Dian Pratiwi
Jurnal Ilmiah Kesehatan Rustida Vol 10 No 1 (2023): Januari
Publisher : Akademi Kesehatan Rustida

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55500/jikr.v10i1.191

Escherichia coli yaitu bakteri yang hidup diusus manusia, sebagai flora normal. Akan tetapi bakteri ini juga sering mengkontaminasi dan dapat menyebabkan penyakit pada manusia. Pengolahan pangan yang tidak hygienis dapat menyebabkan berkembangnya bakteri ini. Penelitian ini bertujuan untuk mengidentifikasi adanya bakteri Coliform pada susu kedelai yang disimpan disuhu kulkas dan suhu ruangan metode MPN (Most Probable Number). penelitian ini bersifat deskriptif analitik dengan populasi susu kedelai yang disimpan disuhu kulkas dan suhu ruangan pada penyimpanan ½, 1, 1 ½, dan 2 hari. Berdasarkan hasil pengujian dengan metode presumptive test, confirmative test dan complete test didapatkan hasil pada penyimpanan disuhu kulkas penyimpanan ½ hari dan 1 hari sampel 1,2,3,4,5 yaitu <3 CFU/mL, penyimpanan 1 ½ hari sampel 1, 2 dan 3 didapatkan 15 CFU/mL, sampel 4 dan 5 yaitu 11 CFU/mL. Penyimpanan 2 hari didapatkan 20 CFU/mL. Sedangkan pada penyimpanan disuhu ruangan ½ hari sebesar <3 CFU/mL, penyimpanan 1 hari pada sampel 1,2 dan 3 yaitu 11 CFU/mL, sampel 4 dan 5 yaitu 7 CFU/mL. Penyimpanan 1 ½ hari didapatkan sampel 1,2 dan 3 yaitu 21 CFU/mL, sampel 4 dan 5 yaitu 20 CFU/mL, penyimpanan 2 hari yaitu 28 CFU/mL. Pada penyimpanan ½ hari dan 1 hari pada penyimpanan disuhu kulkas serta penyimpanan pada suhu ruangan ½ hari dinyatakan tidak ada pengaruh dan layak dikonsumsi serta memenuhi syarat mutu, Sedangkan pada penyimpanan 1 ½ hari dan 2 hari pada suhu kulkas dan 1, 1 ½, dan 2 hari pada suhu ruangan terdapat pengaruh penyimpanan dan tidak memenuhi syarat mutu konsumsi.

SEMINAR IDENTIFIKASI BAKTERI ESCHERICHIA COLI O157:H7 PADA FESES PENDERITA DIARE DENGAN METODE KULTUR DAN PCR Vincentia Ade Rizky; Sa'adah Siregar; Visensius Krisdianilo; Asvia Rahayu
JURNAL PENGMAS KESTRA (JPK) Vol 1 No 1 (2021): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

The technique of identifying Escherichia coli O157: H7 bacteria in the feces of diarrhea sufferers using a molecular method, namely PCR has been proven to be more sensitive and shows fast results but is expensive when compared to conventional methods. Therefore, it can be recommended and used by health workers in early detection so that it will help make the diagnosis faster and determine treatment more effectively. The results of this seminar aim to make it easier for laboratory staff to identify the Escherichia coli O157: H7 bacteria in diarrhea sufferers.

PENGARUH EKSTRAK BAWANG PUTIH (ALLIUM SATIVUM) DENGAN METODE PEMANASAN (BLACK GARLIC) TERHADAP LARVA AEDES AEGYPTI SAADAH SIREGAR; VINCENTIA ADE RIZKY; AINA FITRIA AULIA
Journal of Nursing and Public Health Vol 11 No 1 (2023)
Publisher : UNIVED Press, Universitas Dehasen Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37676/jnph.v11i1.4095

Demam Berdarah Dengue merupakan penyakit tropis yang masih menjadi masalah kesehatan di Indonesia. Untuk mengatasi masalah tersebut perlu dilakukan tindakan preventif dengan menerapkan perilaku pemberantasan sarang nyamuk. Salah satu upaya yang dapat dilakukan adalah dengan melakukan pemberantasan nyamuk aedes aegypti. Tujuan penelitian ini untuk mengetahui efektivitas larutan bawang putih dalam membunuh larva Aedes sp dan menganalisis beda jumlah larva yang mati dari berbagai konsentrasi larutan bawang putih. Jenis penelitian ini adalah penelitian eksperimental. Penelitian ini dilakukan di Kecamatan Teluk Mengkudu. Populasi yang digunakan dalam penelitian ini adalah larva aegypti dari penetasan telur yang diperoleh dari pemasangan ovitrap pada 20 rumah warga pada salah satu wilayah endemis. Jumlah sampel sebanyak 640 larva. Sampel diambil berdasarkan kriteria: Larva aegypti larva hidup dan larva yang bergerak aktif. Analisis yang digunakan adalah uji one way ANOVA. Hasil penelitian menunjukan pemberian larutan bawang putih dengan metode black garlic mempunyai efek larvasida alami terhadap aedes aegypti dengan tingkat mortalitas semakin tinggi pada konsentrasi larutan bawang putih yang lebih tinggi. Konsentrasi 13%, 14%, 15%, 16%, 17%, 18%, 19%, dan 20% merupakan konsentrasi larutan bawang putih dengan metode black garlic yang efektif membunuh 100% larva aedes aegypti Kematian larva aedes aegypti mulai mengalami kenaikan tinggi pada konsentrasi 6%, dan terus mengalami kenaikan hingga konsentrasi 20%. Kematian larva aedes aegypti dari konsentrasi dari bawang putih menunjukan nilai < 0.000. Hal ini menunjukan bahwa tidak terdapat perbedaan yang bermakna antara konsentrasi 5% dengan semua konsentrasi yang diujikan. Pemberian larutan bawang putih dengan metode black garlic mempunyai efek larvasida alami terhadap aedes aegypti Tidak terdapat perbedaan yang bermakna antara konsentrasi 5% dengan semua konsentrasi.

EFEKTIVITAS SEDIAAN GEL ANTISEPTIK TANGAN EKSTRAK DAUN SIRSAK Annona muricata Linn SEBAGAI ANTIBAKTERI TERHADAP Staphylococcus epidermidis VINCENTIA ADE RIZKY; SAADAH SIREGAR; VISENSIUS KRISDIANILO; SITI KHADIJAH
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 9 No. 1 (2024): Bioma : Januari - Juni 2024
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Soursop leaf (Annona muricata Linn) has flavonoid, alkaloid, and saponin compounds that can be used as antibacterial. Based on the compounds contained in soursop leaves can be used as a hand antiseptic. Based on this study, an antiseptic gel formulation of soursop leaf (Annona muricata Linn) as antibacterial against Staphylococcus epidermidis bacteria with a carbomer 940 base was made. Antiseptic gel of soursop leaf extract was formulated with unequal extract concentrations, namely in the 1 3% formulation, the 2.6 % formulation. , formulation 3 9%, and formulation 4 12%. The antibacterial test used was the well diffusion method. The antiseptic gel was tested for its physical properties, namely organoleptic test, homogeneity test, dispersion test, and pH test. Antiseptic gel preparations that have antibacterial activity against Staphylococcus epidermidis bacteria are formulations 3 and 4 9% and 12% extracts which have a picture zone of 5 and 7 mm while the positive control has an inhibition zone of 12 mm. The results of the tests carried out on organoleptic tests, dispersion tests, pH tests, homogeneity tests were in accordance with the parameters of the gel preparation. From this study, it showed the ability of soursop leaves to inhibit the growth of Staphylococcus epidermidis bacteria. Soursop (Annona muricata Linn) leaf extract. Has antibacterial activity against Staphyloccus epidermidis bacteria with a concentration of 3% formulation 1, formulation 6% concentration 2, formulation concentration 9%, formulation concentration 12% 4. At a concentration of 3%, 6% has inhibitory power with a diameter of 3mm, and a concentration of 9% had an inhibitory power with an inhibitory diameter of 5mm, while the concentration of 12% had an inhibitory power with an inhibitory diameter of 7mm against the bacterium Staphyloccus epidermidis.

Pengaruh Keberadaan Jentik dan Perilaku 3M Plus terhadap Kejadian Demam Berdarah Dengue Siregar, Saadah; Mulyani, Sri; Rizky, Vincentia Ade; Akmal, Dzul; Sutriyawan, Agung
Jurnal Kesehatan Komunitas Vol 9 No 3 (2023): Jurnal Kesehatan Komunitas
Publisher : LPPM Hang Tuah Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25311/keskom.Vol9.Iss3.1392

An infectious disease that is still a public health problem today is dengue hemorrhagic fever. Prevention efforts that can be done are by breaking the chain of transmission by eradicating mosquito nests. The purpose of this study is to analyze the influence of the presence of larvae and the behavior of 3M plus on the incidence of dengue hemorrhagic fever. The study used a cross-sectional design. The research was carried out in the working area of Puskesmas Puter, in July - August 2022. The population in this study was 7,110 heads of households. The number of samples was 67 heads of families, taken using stratified random sampling techniques. The statistical test used is the chi-square test. The results showed that the variables that affect the incidence of dengue hemorrhagic fever are the presence of larvae (p = 0.006 and POR = 4.8), closing water reservoirs (p = 0.009 and POR = 4.8), draining water reservoirs (p = 0.041 and POR = 3.5), and larvicides (p = 0.027 and POR = 4.4). This research can conclude that the incidence of DHF can be influenced by several factors, namely the presence of mosquito larvae, and the behavior of closing, draining, and sprinkling larvicide powder. It is recommended that health workers further optimize the role of levels in monitoring mosquito nest eradication behavior carried out by the community.

SOCIALIZATION OF GIVING GINGER TO REDUCING PATHOGENIC AND FOOD-DESTROYING BACTERIA IN CHICKEN MEAT: SOCIALIZATION OF GIVING GINGER TO REDUCING PATHOGENIC AND FOOD-DESTROYING BACTERIA IN CHICKEN MEAT Rizky, Vincentia Ade; Siregar, Saadah; Rahayu, Asvia; Krisdianilo, Visensius
JURNAL PENGMAS KESTRA (JPK) Vol. 2 No. 2 (2022): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Broiler chicken meat is a food source of animal origin that is widely consumed in Indonesia. The affordable price makes the demand for broiler chicken meat increasing. Chicken meat is easily contaminated by various microorganisms from the surrounding environment. Several types of microbes that can contaminate meat include E. Coli bacteria, Clostridium botulinum bacteria, Clostridium Perfringens, Salmonella bacteria, Staphylococcus aureus, and Anthrax. Microbial contamination in the form of Salmonella bacteria is generally found in broiler chicken meat. To overcome microbial contamination, the researchers conducted research on the effect of ginger on reducing the number of bacteria in chicken meat. This socialization aims to provide information to the public to be able to use ginger as an inhibitor and reduce the number of pathogenic bacteria and food destroyers in chicken meat. The results of this socialization were obtained from previous research which concluded that a decrease in the number of bacteria was due to the administration of ginger. This is because ginger contains secondary compounds such as phenols, treponoids and benzaldehyde which are bacteriostatic so that they function to inhibit the growth of microorganisms. The results of the socialization carried out in the community around Lubuk Pakam stated that they understood the processing and storage techniques (100%).

Workshop Diagnosis of Escherichia coli on Well Water Samples Krisdianilo, Visensius; Vincentia Ade Rizky; Saadah Siregar; Rahayu, Asvia
JURNAL PENGMAS KESTRA (JPK) Vol. 3 No. 1 (2023): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jpk.v3i1.1725

Diarrhea is an endemic disease in Indonesia and is also a disease that can potentially cause extraordinary events that are often accompanied by death. Escherichia coli is a commensal bacterium, intestinal pathogen and extraintestinal pathogen that can cause urinary tract infections, meningitis, and septicemia. Most of the E. coli bacteria are in the digestive tract of animals as well as humans and are normal flora, but some are pathogenic that can cause diarrhea in humans (Bettelheim, 2000). Water is very potential in the spread of various diseases, for example water contaminated by pathogens and drunk by humans can cause disease (water born disease). Food and water contamination has been identified as a potential source of the spread of Escherichia coli pathogenicity in humans. The method has the disadvantage of taking a long time, a large number of samples, and an incorrect reading method of results. PCR technique is one of the molecular techniques used to identify infectious diseases caused by Escherchia coli. This method has many advantages that can produce accurate, fast, specific product amplification, requiring a small number of samples. and this method can be used to overcome the weaknesses of conventional diagnostics (cultures). The purpose of this study was to socialize the detection of Escherichia coli bacteria in well water samples by culture and PCR methods. From the examination that has been done obtained the results of cultural examination obtained colonies that grow on the medium Mac Conkey To appear round, flat edges, smooth surface with the color of the colony, then the results of pcr examination obtained 239 bp, which means bacteria found from the isolation of well water samples in the bottom of the identic pakam with Escherichia coli.As well as workshop participants can follow and understand the materials submitted and also the examination procedures carried out.

Workshop on The Use of Miana Leaf Juice (Coleus Scutellarioides (L) Benth) as An Alternative to Safranin Dye in Bacterial Gram Staining Krisdianilo, Visensius; Siregar, Sa'adah; Rahayu, Asvia; Rizky, Vincentia Ade
JURNAL PENGMAS KESTRA (JPK) Vol. 4 No. 1 (2024): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jpk.v4i1.2198

One of the commonly used stains in bacterial identification is gram staining, this staining is used to distinguish the physical properties of the cell wall and the chemical properties of bacteria, this dye will distinguish bacteria into gram-positive bacteria with the result of the bacteria will be purple and gram negative with the result of red bacteria. The use of safranin is effective in working on bacteria because it is alkaline, this can cause binding between the chromophore component in the dye and the cytoplasm of bacteria that are basophilic. However, the use of safranin also has a negative impact on health and the environment. Therefore, the need to use materials that are safer for health and the environment, miana leaf juice (Coleus Scutellarioides (L) Benth) can be used as a natural dye to replace safranin. Minana leaves are very easy to find in gardens and are usually ornamental plants, the anthocyanin content in this plant can be used as a color pigment to replace safranin, anthocyanins will produce red to dark red pigments in an acidic atmosphere. The purpose of this community service activity, the community service organized a workshop for students of the Medical Laboratory Technology Study Program, Lubuk Pakam Medical Health Institute, aiming to provide information on the use of miana leaf juice (Coleus scutellarioides (L) Benth) as an alternative to safranin dye in bacterial gram staining. From the results of the service, it can be concluded that the participants can understand the material and the demonstration, this can be seen from the increase in the percentage of understanding both from before 61% to 87% after being given the demonstration and material.

Co-Authors Adipura Atmadja Egok AINA FITRIA AULIA Akmal, Dzul Anna Teresia Marbun, Romauli Asvia Rahayu Asvia Rahayu Asvia Rahayu Avinda Sari Kanthi Rahayu Awaluddin Awaluddin Awaluddin DALIMUNTHE, HAFIKA Dian Pratiwi Eka Nurul H.P.S Eka Nurul Hidayah Puspa Seruni Hutabarat, Sintya Indriani Indriani Jayanti, Neti Eka Kartini . Krisdianilo, Vinsensius Krisdianilo, Visensius Laili, Ni'mah Hidayatul Lemriani Manullang, Selda Manurung, Arnita Elisabet Maria Selviana Joni Morischa Deyla Sitanggang Nasrullah Tamrin Nasution, Salmiah Neti Eka Jayanti Nopita Sari Manalu, Dian PUTRI, IKKE ELMALIA Rachmat Saleh Rahayu, Asvia Rahmawati Ramadhani, Siti Riska Wahyuni Sa'adah Siregar Saadah Siregar SAADAH SIREGAR SAADAH SIREGAR Saadah Siregar, Saadah Saleh, Rachmat Sa’adah Siregar Siregar, S'aadah Siregar, Sa'adah Siregar, Saâ€™adah Siregar, Sa’adah Sitanggang, Morischa Deyla SITI KHADIJAH Sri Mulyani Suci Mustika Mawardani Sugiyatmi, Tri Astuti Sutriyawan, Agung Syafrina Ginting, Suventi Syukurin Juliani Gulo Tri Astuti Sugiyatmi Umi Latifah Umi Latifah Visensius Krisdianilo Visensius Krisdianilo Visensius Krisdianilo Visensius Krisdianilo, Visensius Wenda Putri Natal Saragih

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