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All Journal Jurnal Kesehatan Komunitas JURNAL FARMASIMED (JFM) Jurnal Ilmiah Kesehatan Rustida Journal SAGO Gizi dan Kesehatan SEHATI: Jurnal Kesehatan Insight Power (Kekuatan Wawasan) - Jurnal Pengabdian Kepada Masyarakat Digital (JUPED) EJOIN : Jurnal Pengabdian Masyarakat Jurnal Pengmas Kestra (JPK) Jurnal Laboratorium Khatulistiwa Indonesian Journal of Community Empowerment Jurnal Medika: Medika Jurnal Sains, Teknologi dan Kesehatan Journal of Medical Surgical Concerns Medistra Medical Journal (MMJ)
Vincentia Ade Rizky
INKES MEDISTRA LUBUK PAKAM
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Analysis Of The Result Of DNA (Deoxyribonucleic Acid) Electrophoresis On Vaginal Mucosal Swabs With Cervical Cancer Patients Ramadhani, Siti; Rizky, Vincentia Ade; Siregar, Sa'adah
Medistra Medical Journal (MMJ) Vol 2 No 1 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i1.2371

Abstract

Background: Cervical cancer is one of the second-highest sexually transmitted diseases in the world, accounting for 13% of the total 22% of deaths caused by non-communicable diseases. Today there have been many developments about cervical cancer diagnosis methods especially in early detection aimed at recognizing cervical cancer at an earlier stage, including molecular biology detection using PCR tools and electrophoresis. Objective: To identify the results of DNA electrophoresis in vaginal mucosal swabs with cervical cancer. Method : The research method used is experimental. Results: Based on the results of a study using a documentary gel, 2 samples of HPV DNA tape were detected, it was tested positive because there was 450 bp DNA tape and 4 samples of negative results were obtained. Conclusion: The frequency distribution of respondents from 6 samples by using electrophoresis and focumerary gel as readers detected 2 positive samples. A positive result is expressed if a result in the documentary gel is found to be a DNA band. The size of the DNA ribbon produced with the DNA marker markers is at 450 bp, according to the sample target. This shows that the DNA bands in the sample are about 450 bp in size.
Utilization of Kluwih Seed Starch (Artocarpus camansi) as an Alternative Growth Medium for Aspergillus niger Fungus siregar, saadah; DALIMUNTHE, HAFIKA; RIZKY, VINCENTIA ADE
Medistra Medical Journal (MMJ) Vol 2 No 1 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i1.2372

Abstract

Background: Carbohydrates in kluwih seeds have sufficient nutrients, such as potatoes and tubers, making it possible to be used as a medium for mushroom growth. The nutritional composition of breadfruit contains 25% carbohydrates, 1.5% protein, and 0.3% fat. Aspergillus niger fungi require nutrients such as carbohydrates and protein to grow. This study aims to determine whether kluwih seed starch can be used as an alternative medium for the growth of Aspergillus niger fungi. Method: This study used a pre-experimental design with laboratory observation. The study was conducted by utilizing kluwih seed starch as a medium, compared to Potato Dextrose Agar (PDA) and Sabouraud Dextrose Agar (SDA). Results: The results showed that kluwih seed starch can be used as an alternative medium for the growth of Aspergillus niger, with fungal colonies that grow macroscopically and microscopically almost the same as PDA and SDA media. Conclusion: Kluwih seed starch can be an alternative substitute for PDA media for the growth of Aspergillus niger.
Modification Of Star Fruit Wuluh (Averrhoa Bilimbi L.) As A Substitute For Turk Solution In The Manual Method Leukocyte Count. Lemriani Manullang, Selda; Ade Rizky, Vincentia; Rahayu, Asvia
Medistra Medical Journal (MMJ) Vol 2 No 2 (2025): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i2.2374

Abstract

Background: This study counts the number of leukocytes using a manual method. The natural materials used are lime, lemon, and tamarind, which contain glacial acid and citric acid with a pH below 2.0. These acids can lyse blood cells, except for leukocytes (white blood cells). Objective: This study aims to analyze the effectiveness of varying concentrations of starfruit (Averrhoa bilimbi) on the number of leukocytes (white blood cells). Method: This research employs an experimental method. The number of leukocytes is counted from the juice of starfruit at concentrations of 5% and 10%. The objective is to find the most effective concentration compared to the standard Turk solution as a control. Conclusion: The data were analyzed using One-Way ANOVA. The average number of leukocytes in the control group was 6,500, in the 10% concentration was 6,450, and in the 5% concentration was 4,500. The results of statistical tests and hypotheses showed a significance value of 0.000, which is less than ? (alpha) (0.05), thus the null hypothesis is rejected and there is a significant difference. The 10% concentration proved to be the most effective in replacing glacial acetic acid in the Turk solution.
WORKSHOP PENGARUH TEPUNG PISANG AMBON (Musa Paradisiaca) SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN JAMUR Aspergillus flavus PENGGANTI MEDIA PDA (Potato Dextrose Agar) Siregar, Sa’adah; Rizky, Vincentia Ade
MAJU : Indonesian Journal of Community Empowerment Vol. 2 No. 3 (2025): MAJU : Indonesian Journal of Community Empowerment, Mei 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/maju.v2i3.1284

Abstract

Fungi play a lot in the life both fungi that are saprophytic and are pathogenic, one that is pathogenisc and produce aflatoxin, the fungus Aspergillus flavus. PDA (Potato Dextrose Agar) is a medium commonly use for fungal growth. PDA medium are usually in the form instan so this media is ready to use. The composition of the PDA, one of which is a potato extract as a source of carbohydrates, so that other subtitutes can be carried out whose composition is almost the same as potatoes, using Ambon banana. Objective: The purpose of this research was to knew the comparison of Aspergillus flavus fungus diameter growth on PDA media and alternatif medium from ambon banana on concentration of 2%, 4% and 6%. Method: The methods this type of research was experimented by way of inoculating Aspergillus flavus with single dot method. Alternative media for ambon banana be used for the growth of the Aspergillus flavus fungus. Result: Data analysis by calculating the growth in the number of colonies growing at each contration shows that there are 85 colonies at a contration of 2%, 133 contration of 4%, and 130 contrations of 6% and there is a paired T-test abtained average results on ambon banana media with successive contrations of 2%, 4%, and 6% namely 77,33, 91,67, 123,33 and on the PDA (Potato Dextrose Agar) control media 92,33.
Real time polymerase chain reaction assay (RT-PCR) sebagai tes cepat mycobacterium tuberculosis dari sampel dahak pasien tuberculosis Krisdianilo, Visensius; Rizky, Vincentia Ade; Siregar, Sa’adah; Rahayu, Asvia
JOURNAL OF Medical Surgical Concerns Vol. 4 No. 1 (2024): June Edition 2024
Publisher : Published by: Indonesian Public Health-Observer Information Forum (IPHORR) Kerjasama dengan Himpunan Perawat Medikal Bedah Indonesia (HIPMEBI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56922/msc.v4i1.371

Abstract

Background: Mycobacterium tuberculosis is known as a highly pathogenic bacterium. These bacteria are aerobic in shape like rods and have resistance to acids. This bacteria can cause tuberculosis (TB). TB disease has been widely recognized as a cause of death which is quite high in the world. Tuberculosis causes the death of nearly one million women each year. Currently, no country in the world is free from tuberculosis. Data shows that Indonesia is the third largest contributor of tuberculosis cases in the world. Examination of mycobacterium tuberculosis bacteria which is routinely carried out in hospitals or health centers uses microscopic diagnosis of acid-resistant bacilli (BTA). Recently, a rapid test of Mycobacterium tuberculosis was carried out using the semi-quantitative Real Time Polymerase Chain Reaction Assay (RT-PCR) method which targets the rpoB gene in Mycobacterium tuberculosis, which can automatically process preparations by extracting doxyribo nucleic acid (DNA) in a cartridge. The time needed for this diagnosis is approximately two hours until the results come out. Purpose: To see the results of the rapid test compared to the microscopic diagnosis at the Puskesmas. Method: Using the cross-sectional method, with a sample of 25 patients at the Lubuk Pakam Health Center. To detect Mycobacterium tuberculosis using the GeneXpert MTB/RIF tool. Furthermore, the data obtained was processed and examined using the Wilcoxon statistical test. Results: The value of the statistical test results using the Wilcoxon test showed that the p-value (Sig) was 0.264, the value was > 0.05, which means that in this study there were no significant differences in results between the microscopic method and RT-PCR. Conclusion: Overall there was no significant difference in the results between microscopy and RT-PCR methods. Keywords: Tuberculosis; Mycobacterium Tuberculosis; RT-PCR Pendahuluan: Mycobacterium tuberculosis dikenal sebagai bakteri yang sangat patogen. Bakteri ini berbentuk aerobik seperti batang dan memiliki ketahanan terhadap asam. Bakteri ini dapat menyebabkan tuberkulosis (TB). Penyakit TBC telah dikenal luas sebagai penyebab kematian yang cukup tinggi di dunia. Tuberkulosis menyebabkan kematian hampir satu juta wanita setiap tahun. Saat ini tidak ada satu negara pun di dunia yang bebas dari tuberkulosis. Data menunjukkan bahwa Indonesia merupakan penyumbang kasus tuberkulosis terbesar ketiga di dunia. Pemeriksaan bakteri mycobacterium tuberculosis yang rutin dilakukan di rumah sakit atau puskesmas menggunakan diagnosis mikroskopis acid-resistant basil (BTA). Baru-baru ini telah dilakukan uji cepat Mycobacterium tuberculosis menggunakan metode semi kuantitatif Real Time Polymerase Chain Reaction Assay (RT-PCR) yang menargetkan gen rpoB pada Mycobacterium tuberculosis, yang secara otomatis dapat memproses preparat dengan ekstraksi asam nukleat doxyribo (DNA) pada catridge. Waktu yang dibutuhkan dalam diagnosis ini adalah kurang lebih dua jam sampai hasilnya keluar. Tujuan: Untuk melihat hasil rapid test tersebut dibandingkan dengan diagnosis mikroskopis pada Puskesmas. Metode: Menggunakan metode  cross sectional, dengan sampel yaitu pasien Puskesmas Lubuk Pakam sebanyak 25 orang. Untuk mendeteksi Mycobacterium tuberculosis menggunakan alat GeneXpert MTB/RIF. Selanjutnya data yang didapat di olah dan diperiksa dengan menggunakan uji statistic Wilcoxon. Hasil: Nilai hasil uji statistic menggunakan uji Wilcoxon didapatkan hasil p-value (Sig) adalah 0.264 nilai tersebut > dari 0.05 yang berarti pada penelitian ini hasilnya tidak terdapat perbedaan hasil yang signifikan antara metode mikroskopis dengan RT-PCR. Simpulan: Secara keseluruhan tidak ada perbedaan yang signifikan dalam hasil antara metode mikroskopis dan RT-PCR.  
WORKSHOP PENGGUNAAN SARI DAUN PACAR KUKU (Lawsonia inermis L) SEBAGAI PENGGANTI SAFRANIN PADA PEWARNAAN GRAM Siregar, Sa’adah; Rizky, Vincentia Ade; Krisdianilo, Visensius; Jayanti, Neti Eka; Sitanggang, Morischa Deyla
EJOIN : Jurnal Pengabdian Masyarakat Vol. 1 No. 12 (2023): EJOIN : Jurnal Pengabdian Masyarakat, Desember 2023
Publisher : LPPM Institut Pendidikan Nusantara Global

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/ejoin.v1i12.1968

Abstract

Gram staining is one way to differentiate gram-positive bacteria and gram negative bacteria. Gram staining in the laboratory is done with using a solution of gentien violet, iodine, alcohol, and safranin. Leaf henna nail (Lawsonia inermis L) is a plant that produces the reddish yellow molecule is called Lawsone. Additionally leaves henna nails It also contains anthocyanins which produce a reddish orange color. This Community Service aims to inform the audience about the benefits of henna leaf extract (Lawsonia inermis L) is effectively used as a substitute for safranin in gram staining. The results of the workshop show that the audience can understand the benefits of henna nail leaf extract as a substitute for safranin in gram staining, but this is only an alternative.
PENYUSUNAN REKOMENDASI KESEHATAN LINGKUNGAN WILAYAH PESISIR BERBASIS DATA SEKUNDER Joni, Maria Selviana; Rizky, Vincentia Ade; Latifah, Umi
MAJU : Indonesian Journal of Community Empowerment Vol. 3 No. 1 (2026): MAJU : Indonesian Journal of Community Empowerment, January 2026
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/maju.v3i1.2276

Abstract

Coastal areas are vulnerable to environmental health problems, particularly those related to sanitation, clean water access, and domestic waste management. Poor environmental conditions contribute to the increased risk of environment-based diseases such as diarrhea and skin infections. This community service activity aimed to develop environmental health recommendations for coastal areas based on secondary data analysis. The method used was descriptive analysis of secondary data obtained from health profiles, statistical reports, and regional development planning documents. The analysis showed gaps between existing environmental health conditions and ideal health standards. Based on these findings, environmental health intervention recommendations focusing on disease prevention, health education, and strengthening primary health services were formulated. These recommendations are expected to support stakeholders in planning sustainable environmental health programs in coastal areas.
Pendampingan Pengelolaan Sampah Berbasis Komunitas Menuju Ecohealthy Campus dan Desa Wisata Sehat Sugiyatmi, Tri Astuti; Saleh, Rachmat; Tamrin, Nasrullah; Seruni, Eka Nurul Hidayah Puspa; Awaluddin, Awaluddin; Egok, Adipura Atmadja; Rizky, Vincentia Ade; Rahayu, Avinda Sari Kanthi; Joni, Maria Selviana; Latifah, Umi; Laili, Ni'mah Hidayatul
Jurnal Medika: Medika Vol. 5 No. 1 (2026)
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/m5mtm120

Abstract

Permasalahan pengelolaan sampah yang belum optimal masih menjadi isu utama di kawasan pesisir Kota Tarakan, khususnya di Kelurahan Pantai Amal yang berfungsi sebagai kawasan wisata sekaligus lokasi Kampus Universitas Borneo Tarakan. Wilayah ini juga merupakan sentra budidaya rumput laut, di mana penggunaan botol plastik sebagai pelampung berkontribusi terhadap timbulan sampah pesisir yang berdampak pada kesehatan lingkungan. Kegiatan pengabdian kepada masyarakat ini bertujuan meningkatkan pengetahuan, kesadaran, dan komitmen sosial petugas kebersihan Universitas Borneo Tarakan serta anggota Kelompok Swadaya Masyarakat (KSM) Tempat Pengolahan Sampah (TPS) 3R (Reduce, Reuse, Recycle) Amal Jaya dalam pengelolaan sampah berbasis prinsip 3R dan kesehatan lingkungan. Metode yang digunakan adalah pendampingan komunitas melalui penyuluhan interaktif, diskusi, pemberian media edukasi, serta demonstrasi pembuatan komposter metode Takakura. Evaluasi dilakukan secara deskriptif menggunakan pre-test dan post-test serta observasi partisipasi peserta. Kegiatan dilaksanakan dalam dua tahap pada 30 September dan 7 Oktober 2025. Hasil kegiatan menunjukkan tingkat pengetahuan peserta berada pada kategori tinggi, disertai peningkatan pemahaman dan keterlibatan aktif. Kegiatan ini menghasilkan komitmen kolektif untuk melanjutkan pendampingan KSM TPS 3R guna mendukung terwujudnya Ecohealthy Campus dan Desa Wisata Sehat Pantai Amal.
Co-Authors Akmal, Dzul Anna Teresia Marbun, Romauli Asvia Rahayu Asvia Rahayu Asvia Rahayu Awaluddin Awaluddin DALIMUNTHE, HAFIKA Dian Pratiwi Egok, Adipura Atmadja Hutabarat, Sintya Indriani Indriani Jayanti, Neti Eka Joni, Maria Selviana Kartini . Krisdianilo, Vinsensius Krisdianilo, Visensius Laili, Ni'mah Hidayatul Lemriani Manullang, Selda Manurung, Arnita Elisabet Morischa Deyla Sitanggang Nasution, Salmiah Neti Eka Jayanti Nopita Sari Manalu, Dian PUTRI, IKKE ELMALIA Rahayu, Asvia Rahayu, Avinda Sari Kanthi Ramadhani, Siti Riska Wahyuni Sa'adah Siregar SAADAH SIREGAR Saadah Siregar, Saadah Saleh, Rachmat Sa’adah Siregar Seruni, Eka Nurul Hidayah Puspa Siregar, S'aadah Siregar, Sa'adah Siregar, Sa’adah Siregar, Sa’adah Sitanggang, Morischa Deyla Sri Mulyani Suci Mustika Mawardani Sugiyatmi, Tri Astuti Sutriyawan, Agung Syafrina Ginting, Suventi Syukurin Juliani Gulo Tamrin, Nasrullah Umi Latifah Visensius Krisdianilo Visensius Krisdianilo Visensius Krisdianilo Visensius Krisdianilo, Visensius Wenda Putri Natal Saragih