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PENGARUH KONSENTRASI MEDIA KACANG KEDELAI(Glycine max L. Merr) DALAM PERTUMBUHAN BAKTERI Staphylococcus aureus  DIBANDINGKAN DENGAN  NUTRIENT Siregar, Sa’adah; Rizky, Vincentia Ade; Nasution, Salmiah
EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan Vol. 2 No. 2 (2025): EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan, Juni 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/empiris.v2i2.1285

Abstract

Soybean is a complete protein, inexpensive and is one of the food ingredients that contain groups of essential and non-essential amino acids, carbohydrates, fats, vitamins and minerals. Staphylococcus is coccus bacteria, gram positive with an irregular arrangement or clusters like grapes. Staphylococcus aureus is a bacterium that is non-spore, non-motile, facultative anaerobe, oxidase negative and catalase positive. The aim of the study was to determine the growth of Staphylococcus aureus bacteria in alternative media for soybeans as a substance for Nutrient agar media. The type of experimental research is to conduct a trial on the concentration of soy bean powder. research  population of soybeans purchased at Lubuk Pakam market. Is an experimental method for the growth of staphylococcus aureus bacteria in soybean media as a substitute for Nutrient agar. Of research from soybeans (Glycine max L. Merr) can be used as an alternative source of protein in the manufacture of alternative media, but have slight differences in the size of the colony, the color of the colony and the color of the pigment produced with varying concentrations of 20%, 40%, 60% and 80%.  Soybeans are effectively used as an alternative protein source to replace Nutrient agar media in growing Staphylococcus aureus bacteria for a more effective concentration, namely at a concentration of 60% when viewed from the number of colonies obtained in the soybean media
WORKSHOP PENGARUH TEPUNG PISANG AMBON (Musa Paradisiaca) SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN JAMUR Aspergillus flavus PENGGANTI MEDIA PDA (Potato Dextrose Agar) Siregar, Sa’adah; Rizky, Vincentia Ade
MAJU : Indonesian Journal of Community Empowerment Vol. 2 No. 3 (2025): MAJU : Indonesian Journal of Community Empowerment, Mei 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/maju.v2i3.1284

Abstract

Fungi play a lot in the life both fungi that are saprophytic and are pathogenic, one that is pathogenisc and produce aflatoxin, the fungus Aspergillus flavus. PDA (Potato Dextrose Agar) is a medium commonly use for fungal growth. PDA medium are usually in the form instan so this media is ready to use. The composition of the PDA, one of which is a potato extract as a source of carbohydrates, so that other subtitutes can be carried out whose composition is almost the same as potatoes, using Ambon banana. Objective: The purpose of this research was to knew the comparison of Aspergillus flavus fungus diameter growth on PDA media and alternatif medium from ambon banana on concentration of 2%, 4% and 6%. Method: The methods this type of research was experimented by way of inoculating Aspergillus flavus with single dot method. Alternative media for ambon banana be used for the growth of the Aspergillus flavus fungus. Result: Data analysis by calculating the growth in the number of colonies growing at each contration shows that there are 85 colonies at a contration of 2%, 133 contration of 4%, and 130 contrations of 6% and there is a paired T-test abtained average results on ambon banana media with successive contrations of 2%, 4%, and 6% namely 77,33, 91,67, 123,33 and on the PDA (Potato Dextrose Agar) control media 92,33.
WORKSHOP PENGGUNAAN SARI DAUN PACAR KUKU (Lawsonia inermis L) SEBAGAI PENGGANTI SAFRANIN PADA PEWARNAAN GRAM Siregar, Sa’adah; Rizky, Vincentia Ade; Krisdianilo, Visensius; Jayanti, Neti Eka; Sitanggang, Morischa Deyla
EJOIN : Jurnal Pengabdian Masyarakat Vol. 1 No. 12 (2023): EJOIN : Jurnal Pengabdian Masyarakat, Desember 2023
Publisher : LPPM Institut Pendidikan Nusantara Global

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/ejoin.v1i12.1968

Abstract

Gram staining is one way to differentiate gram-positive bacteria and gram negative bacteria. Gram staining in the laboratory is done with using a solution of gentien violet, iodine, alcohol, and safranin. Leaf henna nail (Lawsonia inermis L) is a plant that produces the reddish yellow molecule is called Lawsone. Additionally leaves henna nails It also contains anthocyanins which produce a reddish orange color. This Community Service aims to inform the audience about the benefits of henna leaf extract (Lawsonia inermis L) is effectively used as a substitute for safranin in gram staining. The results of the workshop show that the audience can understand the benefits of henna nail leaf extract as a substitute for safranin in gram staining, but this is only an alternative.
POTENSI SENYAWA BIOAKTIF DARI EUCHEUMA COTTONII TERHADAP GEN NS5 DAN ENVELOPE (E) VIRUS DENGUE SERTA TARGET ACHE DAN OBP PADA AEDES AEGYPTI: TINJAUAN MINI Vincentia Ade Rizky; Maria Selviana Joni; Umi Latifah; Ni’mah Hidayatul Laili; Eka Nurul H.P.S; Avinda Sari Kanthi Rahayu; Rachmat Saleh; Nasrullah Tamrin; Awaluddin; Adipura Atmadja Egok; Tri Astuti Sugiyatmi
EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan Vol. 2 No. 4 (2025): EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan, Desember 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/empiris.v2i4.2015

Abstract

Dengue fever remains a major global health concern, especially in tropical regions, due to increasing insecticide resistance and the absence of specific antiviral treatments. Marine-derived natural products, such as those from Eucheuma cottonii, offer promising alternatives. This mini-review aims to evaluate the potential of E. cottonii bioactive compounds targeting molecular markers of Aedes aegypti mosquitoes and the Dengue virus (DENV). A review of 30 relevant national and international articles published between 2015 and 2025 was conducted, focusing on the larvicidal and antiviral activities of E. cottonii. E. cottonii contains flavonoids, alkaloids, terpenoids, and sulfated polysaccharides. These compounds inhibit AChE and OBP enzymes in mosquito larvae and target the NS5 and Envelope (E) proteins of DENV. The antiviral effects are also enhanced by its antioxidant and immunomodulatory activities. Eucheuma cottonii has a significant potential as a multifunctional natural resource for sustainable strategies in dengue control.
Penerapan Perilaku Hidup Bersih dan Sehat pada Karyawan Embung Persemaian di Kota Tarakan Maria Selviana Joni; Vincentia Ade Rizky; Umi Latifah; Tri Astuti Sugiyatmi
Jurnal Kabar Masyarakat Vol. 4 No. 2 (2026): Mei: JURNAL KABAR MASYARAKAT
Publisher : Institut Teknologi dan Bisnis Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54066/jkb.v4i2.3804

Abstract

Clean and Healthy Living Behavior (PHBS) is a promotive and preventive effort that aims to improve the degree of public health through behavior change. Nursery reservoir employees have a high risk of exposure to the work environment in the form of dirty water, biological waste, and pathogenic microorganisms. This community service activity was carried out to increase the knowledge and practice of PHBS among employees of the nursery reservoir in Juata Tarakan based on national PHBS indicators. The implementation method includes counseling, demonstration, and evaluation using pre-test and post-test. The results showed a significant increase in employee knowledge, with an average pre-test score of 58.7 increasing to 78.3 in the post-test. The statistical test resulted in a value of p = 0.000 (p < 0.05), which confirms the effectiveness of PHBS educational interventions in improving workers' health knowledge and behavior. These findings provide practical implications that counseling-based education and demonstrations can be an effective strategy in encouraging the implementation of PHBS in risky work environments. Thus, this activity is expected to be able to support the creation of a healthier work environment, increase productivity, and become a model for community-based occupational health interventions.
Potensi Larvasida Tanaman Endemik Kalimantan terhadap Aedes aegypti: Tinjauan Evidensi Ilmiah dan Prioritas Kandidat Riset Vincentia Ade Rizky; Maria Selviana Joni; Umi Latifah; Tri Astuti Sugiyatmi
JURNAL RISET RUMPUN MATEMATIKA DAN ILMU PENGETAHUAN ALAM Vol. 5 No. 1 (2026): April : JURRIMIPA: Jurnal Riset Rumpun Matematika dan Ilmu Pengetahuan Alam
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jurrimipa.v5i1.9131

Abstract

Dengue remains a major vector-borne disease worldwide, and the increasing resistance of Aedes aegypti to synthetic insecticides has encouraged the exploration of environmentally friendly botanical larvicides. This study aimed to systematically evaluate scientific evidence regarding the larvicidal potential of endemic Kalimantan plants against Aedes aegypti and to identify priority candidates for future research. A systematic literature review was conducted following the PRISMA 2020 guidelines. Literature searches were performed across Scopus, PubMed, ScienceDirect, SpringerLink, Web of Science, MDPI, Wiley Online Library, Garuda, SINTA, and Google Scholar for studies published between January 2016 and January 2026. A total of 925 records were identified, and after duplicate removal, screening, and eligibility assessment, 88 studies were included in the final synthesis. The results showed that botanical larvicides rich in alkaloids, flavonoids, terpenoids, phenolics, limonoids, and sulfur compounds demonstrated significant larvicidal activity against Aedes aegypti. However, no direct larvicidal studies were identified for endemic Kalimantan species, including Fibraurea tinctoria, Arcangelisia flava, Dacrydium beccarii, Agathis borneensis, and Scorodocarpus borneensis. These findings highlight substantial evidence gaps and indicate that Kalimantan biodiversity represents a promising source of novel larvicidal agents for sustainable dengue vector control.
Pendampingan Pengelolaan Sampah Berbasis Komunitas Menuju Ecohealthy Campus dan Desa Wisata Sehat Tri Astuti Sugiyatmi; Rachmat Saleh; Nasrullah Tamrin; Eka Nurul Hidayah Puspa Seruni; Awaluddin Awaluddin; Adipura Atmadja Egok; Vincentia Ade Rizky; Avinda Sari Kanthi Rahayu; Maria Selviana Joni; Umi Latifah; Ni&#039;mah Hidayatul Laili
Jurnal Medika: Medika Vol. 5 No. 1 (2026)
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/m5mtm120

Abstract

Permasalahan pengelolaan sampah yang belum optimal masih menjadi isu utama di kawasan pesisir Kota Tarakan, khususnya di Kelurahan Pantai Amal yang berfungsi sebagai kawasan wisata sekaligus lokasi Kampus Universitas Borneo Tarakan. Wilayah ini juga merupakan sentra budidaya rumput laut, di mana penggunaan botol plastik sebagai pelampung berkontribusi terhadap timbulan sampah pesisir yang berdampak pada kesehatan lingkungan. Kegiatan pengabdian kepada masyarakat ini bertujuan meningkatkan pengetahuan, kesadaran, dan komitmen sosial petugas kebersihan Universitas Borneo Tarakan serta anggota Kelompok Swadaya Masyarakat (KSM) Tempat Pengolahan Sampah (TPS) 3R (Reduce, Reuse, Recycle) Amal Jaya dalam pengelolaan sampah berbasis prinsip 3R dan kesehatan lingkungan. Metode yang digunakan adalah pendampingan komunitas melalui penyuluhan interaktif, diskusi, pemberian media edukasi, serta demonstrasi pembuatan komposter metode Takakura. Evaluasi dilakukan secara deskriptif menggunakan pre-test dan post-test serta observasi partisipasi peserta. Kegiatan dilaksanakan dalam dua tahap pada 30 September dan 7 Oktober 2025. Hasil kegiatan menunjukkan tingkat pengetahuan peserta berada pada kategori tinggi, disertai peningkatan pemahaman dan keterlibatan aktif. Kegiatan ini menghasilkan komitmen kolektif untuk melanjutkan pendampingan KSM TPS 3R guna mendukung terwujudnya Ecohealthy Campus dan Desa Wisata Sehat Pantai Amal.
Pewarnaan alternatif alami daun miana (Coleus scutellarioides (L) benth) sebagai pengganti gentian violet pada pewarnaan gram bakteri staphylococcus aureus VINCENTIA ADE RIZKY; SAADAH SIREGAR; VISENSIUS KRISDIANILO; RISKA WAHYUNI
Medistra Medical Journal (MMJ) Vol 1 No 2 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v1i2.2095

Abstract

Daun miana (Coleus scutellarioides (L) benth) yang memiliki corak ungu kemerahan mengindifikasikan terdapat antosianin, salah satu variannya yaitu crispa. Penelitian ini bertujuan untuk menguji zat warna antosianin pada sari daun miana sebagai pengganti gentian violet pada pewarnaan gram, Jenis penelitian yang digunakan adalah eksperimental/eksperimen. penelitian ini bertujuan untuk mengetahui apakah sari daun miana (Coleus scutellarioides (L) benth) mampu sebagai bahan alternatif pengganti reagen gentian violet pada bakteri Staphylococcus aureus. Dan pada penelitian ini dilakukan perasan sari daun miana (Coleus scutellarioides (L) benth). sampel bakteri Staphylococcus aureus dibuat sediaan preparat dan pewarnaan menggunakan gentian violet sebagai bahan kontrol dan menggunakan sari daun miana (Coleus scutellarioides (L) benth) sebagai eksperimen menggunakan konsentrasi sari daun miana yaitu 1%, 0,1% dan juga 0,5%. Data yang diperoleh diolah mengggunakan analisa data deskriftif. Hasil penelitian pada sari daun miana sari konsentrasi 1%, dan 0,1% mampu mewarnai bakteri Staphylococcus aureus dibandingan dengan konsentrasi 0,5%. Penelitian ini dapat juga dikembangkan dengan uji coba pada daun lain yang memiliki kandungan antosianin dan perlu juga melalukan konsentrasi yang lebih tinggi atau pun menggunakan metode yang lain.
Utilization of Lemon Juice (Citrus limon L.) as an Alternative Substitute for Sodium Citrate in Blood Sedimentation Rate Examination VINCENTIA ADE RIZKY; IKKE ELMALIA PUTRI; SAADAH SIREGAR
Medistra Medical Journal (MMJ) Vol 2 No 1 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i1.2370

Abstract

Background: The erythrocyte sedimentation rate (LED) examination is a hematological test that describes the rate at which red blood cells settle in plasma, using 3.8% sodium citrate anticoagulant and is measured in mm/hour. Lemon (Citrus limon L.) is a plant with natural properties as a metal binding agent or chelating agent. Lemons contain vitamin C, citric acid, potassium, and limonene. Citric acid compounds are able to form complex bonds with metals because they have COOH and -OH functional groups. Apart from citric acid, lemons contain flavonoids which function as antiviral, anticancer and antiallergenic. Objective: To test whether lemon juice (Citrus limon L.) can be used as an alternative ingredient to replace sodium citrate in LED tests. Method: This research is an experiment using the Westergreen method. Results: LED examination with 3.8% sodium citrate produced a value of 10 mm/hour. Lemon juice with a concentration of 3.8% produces a value of 15 mm/hour, 10% produces a value of 10 mm/hour, 50% produces a value of 18 mm/hour, and 100% produces a value of 0 mm/hour. Conclusion: A lemon juice concentration of 10% showed results closest to the control, with similar LED values. Thus, lemon juice (Citrus limon L.) can serve as a substitute for sodium citrate in the LED test
Analysis Of The Result Of DNA (Deoxyribonucleic Acid) Electrophoresis On Vaginal Mucosal Swabs With Cervical Cancer Patients Siti Ramadhani; Vincentia Ade Rizky; Sa'adah Siregar
Medistra Medical Journal (MMJ) Vol 2 No 1 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i1.2371

Abstract

Background: Cervical cancer is one of the second-highest sexually transmitted diseases in the world, accounting for 13% of the total 22% of deaths caused by non-communicable diseases. Today there have been many developments about cervical cancer diagnosis methods especially in early detection aimed at recognizing cervical cancer at an earlier stage, including molecular biology detection using PCR tools and electrophoresis. Objective: To identify the results of DNA electrophoresis in vaginal mucosal swabs with cervical cancer. Method : The research method used is experimental. Results: Based on the results of a study using a documentary gel, 2 samples of HPV DNA tape were detected, it was tested positive because there was 450 bp DNA tape and 4 samples of negative results were obtained. Conclusion: The frequency distribution of respondents from 6 samples by using electrophoresis and focumerary gel as readers detected 2 positive samples. A positive result is expressed if a result in the documentary gel is found to be a DNA band. The size of the DNA ribbon produced with the DNA marker markers is at 450 bp, according to the sample target. This shows that the DNA bands in the sample are about 450 bp in size.