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All Journal BIOMA : Jurnal Biologi Makassar Jurnal Kesehatan Komunitas JURNAL FARMASIMED (JFM) Jurnal Ilmiah Kesehatan Rustida Journal of Nursing and Public Health (JNPH) Journal SAGO Gizi dan Kesehatan SEHATI: Jurnal Kesehatan Jurnal Riset Rumpun Matematika dan Ilmu Pengetahuan Alam (JURRIMIPA) Insight Power (Kekuatan Wawasan) - Jurnal Pengabdian Kepada Masyarakat Digital (JUPED) EJOIN : Jurnal Pengabdian Masyarakat Jurnal Pengmas Kestra (JPK) Jurnal Kabar Masyarakat Jurnal Laboratorium Khatulistiwa Indonesian Journal of Community Empowerment Jurnal Medika: Medika Jurnal Sains, Teknologi dan Kesehatan Science: Indonesian Journal of Science Journal of Medical Surgical Concerns Medistra Medical Journal (MMJ)
Vincentia Ade Rizky
Universitas Borneo Tarakan
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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PENGARUH KONSENTRASI MEDIA KACANG KEDELAI(Glycine max L. Merr) DALAM PERTUMBUHAN BAKTERI Staphylococcus aureus  DIBANDINGKAN DENGAN  NUTRIENT Siregar, Sa’adah; Rizky, Vincentia Ade; Nasution, Salmiah
EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan Vol. 2 No. 2 (2025): EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan, Juni 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/empiris.v2i2.1285

Abstract

Soybean is a complete protein, inexpensive and is one of the food ingredients that contain groups of essential and non-essential amino acids, carbohydrates, fats, vitamins and minerals. Staphylococcus is coccus bacteria, gram positive with an irregular arrangement or clusters like grapes. Staphylococcus aureus is a bacterium that is non-spore, non-motile, facultative anaerobe, oxidase negative and catalase positive. The aim of the study was to determine the growth of Staphylococcus aureus bacteria in alternative media for soybeans as a substance for Nutrient agar media. The type of experimental research is to conduct a trial on the concentration of soy bean powder. research  population of soybeans purchased at Lubuk Pakam market. Is an experimental method for the growth of staphylococcus aureus bacteria in soybean media as a substitute for Nutrient agar. Of research from soybeans (Glycine max L. Merr) can be used as an alternative source of protein in the manufacture of alternative media, but have slight differences in the size of the colony, the color of the colony and the color of the pigment produced with varying concentrations of 20%, 40%, 60% and 80%.  Soybeans are effectively used as an alternative protein source to replace Nutrient agar media in growing Staphylococcus aureus bacteria for a more effective concentration, namely at a concentration of 60% when viewed from the number of colonies obtained in the soybean media
Pewarnaan alternatif alami daun miana (Coleus scutellarioides (L) benth) sebagai pengganti gentian violet pada pewarnaan gram bakteri staphylococcus aureus RIZKY, VINCENTIA ADE; SIREGAR, SAADAH; KRISDIANILO, VISENSIUS; RISKA WAHYUNI
Medistra Medical Journal (MMJ) Vol 1 No 2 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v1i2.2095

Abstract

Daun miana (Coleus scutellarioides (L) benth) yang memiliki corak ungu kemerahan mengindifikasikan terdapat antosianin, salah satu variannya yaitu crispa. Penelitian ini bertujuan untuk menguji zat warna antosianin pada sari daun miana sebagai pengganti gentian violet pada pewarnaan gram, Jenis penelitian yang digunakan adalah eksperimental/eksperimen. penelitian ini bertujuan untuk mengetahui apakah sari daun miana (Coleus scutellarioides (L) benth) mampu sebagai bahan alternatif pengganti reagen gentian violet pada bakteri Staphylococcus aureus. Dan pada penelitian ini dilakukan perasan sari daun miana (Coleus scutellarioides (L) benth). sampel bakteri Staphylococcus aureus dibuat sediaan preparat dan pewarnaan menggunakan gentian violet sebagai bahan kontrol dan menggunakan sari daun miana (Coleus scutellarioides (L) benth) sebagai eksperimen menggunakan konsentrasi sari daun miana yaitu 1%, 0,1% dan juga 0,5%. Data yang diperoleh diolah mengggunakan analisa data deskriftif. Hasil penelitian pada sari daun miana sari konsentrasi 1%, dan 0,1% mampu mewarnai bakteri Staphylococcus aureus dibandingan dengan konsentrasi 0,5%. Penelitian ini dapat juga dikembangkan dengan uji coba pada daun lain yang memiliki kandungan antosianin dan perlu juga melalukan konsentrasi yang lebih tinggi atau pun menggunakan metode yang lain.
Utilization of Lemon Juice (Citrus limon L.) as an Alternative Substitute for Sodium Citrate in Blood Sedimentation Rate Examination RIZKY, VINCENTIA ADE; PUTRI, IKKE ELMALIA; SIREGAR, SAADAH
Medistra Medical Journal (MMJ) Vol 2 No 1 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i1.2370

Abstract

Background: The erythrocyte sedimentation rate (LED) examination is a hematological test that describes the rate at which red blood cells settle in plasma, using 3.8% sodium citrate anticoagulant and is measured in mm/hour. Lemon (Citrus limon L.) is a plant with natural properties as a metal binding agent or chelating agent. Lemons contain vitamin C, citric acid, potassium, and limonene. Citric acid compounds are able to form complex bonds with metals because they have COOH and -OH functional groups. Apart from citric acid, lemons contain flavonoids which function as antiviral, anticancer and antiallergenic. Objective: To test whether lemon juice (Citrus limon L.) can be used as an alternative ingredient to replace sodium citrate in LED tests. Method: This research is an experiment using the Westergreen method. Results: LED examination with 3.8% sodium citrate produced a value of 10 mm/hour. Lemon juice with a concentration of 3.8% produces a value of 15 mm/hour, 10% produces a value of 10 mm/hour, 50% produces a value of 18 mm/hour, and 100% produces a value of 0 mm/hour. Conclusion: A lemon juice concentration of 10% showed results closest to the control, with similar LED values. Thus, lemon juice (Citrus limon L.) can serve as a substitute for sodium citrate in the LED test
Analysis Of The Result Of DNA (Deoxyribonucleic Acid) Electrophoresis On Vaginal Mucosal Swabs With Cervical Cancer Patients Ramadhani, Siti; Rizky, Vincentia Ade; Siregar, Sa'adah
Medistra Medical Journal (MMJ) Vol 2 No 1 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i1.2371

Abstract

Background: Cervical cancer is one of the second-highest sexually transmitted diseases in the world, accounting for 13% of the total 22% of deaths caused by non-communicable diseases. Today there have been many developments about cervical cancer diagnosis methods especially in early detection aimed at recognizing cervical cancer at an earlier stage, including molecular biology detection using PCR tools and electrophoresis. Objective: To identify the results of DNA electrophoresis in vaginal mucosal swabs with cervical cancer. Method : The research method used is experimental. Results: Based on the results of a study using a documentary gel, 2 samples of HPV DNA tape were detected, it was tested positive because there was 450 bp DNA tape and 4 samples of negative results were obtained. Conclusion: The frequency distribution of respondents from 6 samples by using electrophoresis and focumerary gel as readers detected 2 positive samples. A positive result is expressed if a result in the documentary gel is found to be a DNA band. The size of the DNA ribbon produced with the DNA marker markers is at 450 bp, according to the sample target. This shows that the DNA bands in the sample are about 450 bp in size.
Utilization of Kluwih Seed Starch (Artocarpus camansi) as an Alternative Growth Medium for Aspergillus niger Fungus siregar, saadah; DALIMUNTHE, HAFIKA; RIZKY, VINCENTIA ADE
Medistra Medical Journal (MMJ) Vol 2 No 1 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i1.2372

Abstract

Background: Carbohydrates in kluwih seeds have sufficient nutrients, such as potatoes and tubers, making it possible to be used as a medium for mushroom growth. The nutritional composition of breadfruit contains 25% carbohydrates, 1.5% protein, and 0.3% fat. Aspergillus niger fungi require nutrients such as carbohydrates and protein to grow. This study aims to determine whether kluwih seed starch can be used as an alternative medium for the growth of Aspergillus niger fungi. Method: This study used a pre-experimental design with laboratory observation. The study was conducted by utilizing kluwih seed starch as a medium, compared to Potato Dextrose Agar (PDA) and Sabouraud Dextrose Agar (SDA). Results: The results showed that kluwih seed starch can be used as an alternative medium for the growth of Aspergillus niger, with fungal colonies that grow macroscopically and microscopically almost the same as PDA and SDA media. Conclusion: Kluwih seed starch can be an alternative substitute for PDA media for the growth of Aspergillus niger.
Modification Of Star Fruit Wuluh (Averrhoa Bilimbi L.) As A Substitute For Turk Solution In The Manual Method Leukocyte Count. Lemriani Manullang, Selda; Ade Rizky, Vincentia; Rahayu, Asvia
Medistra Medical Journal (MMJ) Vol 2 No 2 (2025): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i2.2374

Abstract

Background: This study counts the number of leukocytes using a manual method. The natural materials used are lime, lemon, and tamarind, which contain glacial acid and citric acid with a pH below 2.0. These acids can lyse blood cells, except for leukocytes (white blood cells). Objective: This study aims to analyze the effectiveness of varying concentrations of starfruit (Averrhoa bilimbi) on the number of leukocytes (white blood cells). Method: This research employs an experimental method. The number of leukocytes is counted from the juice of starfruit at concentrations of 5% and 10%. The objective is to find the most effective concentration compared to the standard Turk solution as a control. Conclusion: The data were analyzed using One-Way ANOVA. The average number of leukocytes in the control group was 6,500, in the 10% concentration was 6,450, and in the 5% concentration was 4,500. The results of statistical tests and hypotheses showed a significance value of 0.000, which is less than ? (alpha) (0.05), thus the null hypothesis is rejected and there is a significant difference. The 10% concentration proved to be the most effective in replacing glacial acetic acid in the Turk solution.
Comparison Of Potato (Solanum tuerosum L) and Corn (Zea mays) as Alternative Media To Nutrient Agar Media In The Groth of Staphylococcus aureus Bacteria Rahmawati; Siregar, Sa’adah; Ade Rizky , Vincentia
Medistra Medical Journal (MMJ) Vol 2 No 1 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i1.2376

Abstract

Background: Microbiology is the study of microorganisms, which are microscopic organisms that can only be observed through a microscope. These microorganisms have an important role in various aspects of life, including in the health sector. Objective: This study aims to evaluate whether alternative media, such as potato and corn, can be used as a substitute for Nutrient Agar in supporting the growth of Staphylococcus aureus bacteria. Methods: This research is experimental, by comparing the effectiveness of potato and corn media as an alternative to Nutrient Agar for the growth of Staphylococcus aureus. Results: The results of the research in the One-way Anova test obtained a significant value (0.00) which means (p <0.05), so it can be concluded that there is a significant difference between the ability of Nutrient Agar media, potato media and corn media in growing Staphylococcus aureus bacteria. To find out each group that has a significant difference, the Post-hoc follow-up test is carried out. The results of the post-hoc follow-up test shown in Figure 4.2.2 obtained a significant value between Nutrient Agar media and potato media (0.00), Nutrient Agar media with corn media (0.558), and between potato and corn media (0.00). Conclusion: The results showed that potato media was significantly different in its ability to grow Staphylococcus aureus compared to Nutrient Agar and corn. Potato media shows potential as an alternative to Nutrient Agar, despite its different effectiveness.
WORKSHOP PENGARUH TEPUNG PISANG AMBON (Musa Paradisiaca) SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN JAMUR Aspergillus flavus PENGGANTI MEDIA PDA (Potato Dextrose Agar) Siregar, Sa’adah; Rizky, Vincentia Ade
MAJU : Indonesian Journal of Community Empowerment Vol. 2 No. 3 (2025): MAJU : Indonesian Journal of Community Empowerment, Mei 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/maju.v2i3.1284

Abstract

Fungi play a lot in the life both fungi that are saprophytic and are pathogenic, one that is pathogenisc and produce aflatoxin, the fungus Aspergillus flavus. PDA (Potato Dextrose Agar) is a medium commonly use for fungal growth. PDA medium are usually in the form instan so this media is ready to use. The composition of the PDA, one of which is a potato extract as a source of carbohydrates, so that other subtitutes can be carried out whose composition is almost the same as potatoes, using Ambon banana. Objective: The purpose of this research was to knew the comparison of Aspergillus flavus fungus diameter growth on PDA media and alternatif medium from ambon banana on concentration of 2%, 4% and 6%. Method: The methods this type of research was experimented by way of inoculating Aspergillus flavus with single dot method. Alternative media for ambon banana be used for the growth of the Aspergillus flavus fungus. Result: Data analysis by calculating the growth in the number of colonies growing at each contration shows that there are 85 colonies at a contration of 2%, 133 contration of 4%, and 130 contrations of 6% and there is a paired T-test abtained average results on ambon banana media with successive contrations of 2%, 4%, and 6% namely 77,33, 91,67, 123,33 and on the PDA (Potato Dextrose Agar) control media 92,33.
Real time polymerase chain reaction assay (RT-PCR) sebagai tes cepat mycobacterium tuberculosis dari sampel dahak pasien tuberculosis Krisdianilo, Visensius; Rizky, Vincentia Ade; Siregar, Sa’adah; Rahayu, Asvia
JOURNAL OF Medical Surgical Concerns Vol. 4 No. 1 (2024): June Edition 2024
Publisher : Published by: Indonesian Public Health-Observer Information Forum (IPHORR) Kerjasama dengan Himpunan Perawat Medikal Bedah Indonesia (HIPMEBI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56922/msc.v4i1.371

Abstract

Background: Mycobacterium tuberculosis is known as a highly pathogenic bacterium. These bacteria are aerobic in shape like rods and have resistance to acids. This bacteria can cause tuberculosis (TB). TB disease has been widely recognized as a cause of death which is quite high in the world. Tuberculosis causes the death of nearly one million women each year. Currently, no country in the world is free from tuberculosis. Data shows that Indonesia is the third largest contributor of tuberculosis cases in the world. Examination of mycobacterium tuberculosis bacteria which is routinely carried out in hospitals or health centers uses microscopic diagnosis of acid-resistant bacilli (BTA). Recently, a rapid test of Mycobacterium tuberculosis was carried out using the semi-quantitative Real Time Polymerase Chain Reaction Assay (RT-PCR) method which targets the rpoB gene in Mycobacterium tuberculosis, which can automatically process preparations by extracting doxyribo nucleic acid (DNA) in a cartridge. The time needed for this diagnosis is approximately two hours until the results come out. Purpose: To see the results of the rapid test compared to the microscopic diagnosis at the Puskesmas. Method: Using the cross-sectional method, with a sample of 25 patients at the Lubuk Pakam Health Center. To detect Mycobacterium tuberculosis using the GeneXpert MTB/RIF tool. Furthermore, the data obtained was processed and examined using the Wilcoxon statistical test. Results: The value of the statistical test results using the Wilcoxon test showed that the p-value (Sig) was 0.264, the value was > 0.05, which means that in this study there were no significant differences in results between the microscopic method and RT-PCR. Conclusion: Overall there was no significant difference in the results between microscopy and RT-PCR methods. Keywords: Tuberculosis; Mycobacterium Tuberculosis; RT-PCR Pendahuluan: Mycobacterium tuberculosis dikenal sebagai bakteri yang sangat patogen. Bakteri ini berbentuk aerobik seperti batang dan memiliki ketahanan terhadap asam. Bakteri ini dapat menyebabkan tuberkulosis (TB). Penyakit TBC telah dikenal luas sebagai penyebab kematian yang cukup tinggi di dunia. Tuberkulosis menyebabkan kematian hampir satu juta wanita setiap tahun. Saat ini tidak ada satu negara pun di dunia yang bebas dari tuberkulosis. Data menunjukkan bahwa Indonesia merupakan penyumbang kasus tuberkulosis terbesar ketiga di dunia. Pemeriksaan bakteri mycobacterium tuberculosis yang rutin dilakukan di rumah sakit atau puskesmas menggunakan diagnosis mikroskopis acid-resistant basil (BTA). Baru-baru ini telah dilakukan uji cepat Mycobacterium tuberculosis menggunakan metode semi kuantitatif Real Time Polymerase Chain Reaction Assay (RT-PCR) yang menargetkan gen rpoB pada Mycobacterium tuberculosis, yang secara otomatis dapat memproses preparat dengan ekstraksi asam nukleat doxyribo (DNA) pada catridge. Waktu yang dibutuhkan dalam diagnosis ini adalah kurang lebih dua jam sampai hasilnya keluar. Tujuan: Untuk melihat hasil rapid test tersebut dibandingkan dengan diagnosis mikroskopis pada Puskesmas. Metode: Menggunakan metode  cross sectional, dengan sampel yaitu pasien Puskesmas Lubuk Pakam sebanyak 25 orang. Untuk mendeteksi Mycobacterium tuberculosis menggunakan alat GeneXpert MTB/RIF. Selanjutnya data yang didapat di olah dan diperiksa dengan menggunakan uji statistic Wilcoxon. Hasil: Nilai hasil uji statistic menggunakan uji Wilcoxon didapatkan hasil p-value (Sig) adalah 0.264 nilai tersebut > dari 0.05 yang berarti pada penelitian ini hasilnya tidak terdapat perbedaan hasil yang signifikan antara metode mikroskopis dengan RT-PCR. Simpulan: Secara keseluruhan tidak ada perbedaan yang signifikan dalam hasil antara metode mikroskopis dan RT-PCR.  
WORKSHOP PENGGUNAAN SARI DAUN PACAR KUKU (Lawsonia inermis L) SEBAGAI PENGGANTI SAFRANIN PADA PEWARNAAN GRAM Siregar, Sa’adah; Rizky, Vincentia Ade; Krisdianilo, Visensius; Jayanti, Neti Eka; Sitanggang, Morischa Deyla
EJOIN : Jurnal Pengabdian Masyarakat Vol. 1 No. 12 (2023): EJOIN : Jurnal Pengabdian Masyarakat, Desember 2023
Publisher : LPPM Institut Pendidikan Nusantara Global

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/ejoin.v1i12.1968

Abstract

Gram staining is one way to differentiate gram-positive bacteria and gram negative bacteria. Gram staining in the laboratory is done with using a solution of gentien violet, iodine, alcohol, and safranin. Leaf henna nail (Lawsonia inermis L) is a plant that produces the reddish yellow molecule is called Lawsone. Additionally leaves henna nails It also contains anthocyanins which produce a reddish orange color. This Community Service aims to inform the audience about the benefits of henna leaf extract (Lawsonia inermis L) is effectively used as a substitute for safranin in gram staining. The results of the workshop show that the audience can understand the benefits of henna nail leaf extract as a substitute for safranin in gram staining, but this is only an alternative.
Co-Authors Adipura Atmadja Egok AINA FITRIA AULIA Akmal, Dzul Anna Teresia Marbun, Romauli Asvia Rahayu Asvia Rahayu Asvia Rahayu Avinda Sari Kanthi Rahayu Awaluddin Awaluddin Awaluddin DALIMUNTHE, HAFIKA Dian Pratiwi Eka Nurul H.P.S Eka Nurul Hidayah Puspa Seruni HPS, Eka Nurul Hutabarat, Sintya Indriani Indriani Jayanti, Neti Eka Kartini . Krisdianilo, Vinsensius Krisdianilo, Visensius Laili, Ni'mah Hidayatul Lemriani Manullang, Selda Manurung, Arnita Elisabet Maria Selviana Joni Morischa Deyla Sitanggang Nasrullah Tamrin Nasution, Salmiah Neti Eka Jayanti Nopita Sari Manalu, Dian PUTRI, IKKE ELMALIA Rachmat Saleh Rahayu, Asvia Rahmawati Ramadhani, Siti Riska Wahyuni Sa'adah Siregar SAADAH SIREGAR Saadah Siregar SAADAH SIREGAR Saadah Siregar, Saadah Saleh, Rachmat Sa’adah Siregar Siregar, S'aadah Siregar, Sa'adah Siregar, Sa’adah Siregar, Sa’adah Sitanggang, Morischa Deyla SITI KHADIJAH Sri Mulyani Suci Mustika Mawardani Sugiyatmi, Tri Astuti Sutriyawan, Agung Syafrina Ginting, Suventi Syukurin Juliani Gulo Tri Astuti Sugiyatmi Umi Latifah Umi Latifah Visensius Krisdianilo Visensius Krisdianilo Visensius Krisdianilo Visensius Krisdianilo, Visensius Wenda Putri Natal Saragih