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All Journal Jurnal Kesehatan Komunitas JURNAL FARMASIMED (JFM) Jurnal Ilmiah Kesehatan Rustida Journal SAGO Gizi dan Kesehatan SEHATI: Jurnal Kesehatan Insight Power (Kekuatan Wawasan) - Jurnal Pengabdian Kepada Masyarakat Digital (JUPED) EJOIN : Jurnal Pengabdian Masyarakat Jurnal Pengmas Kestra (JPK) Jurnal Laboratorium Khatulistiwa Indonesian Journal of Community Empowerment Jurnal Sains, Teknologi dan Kesehatan Journal of Medical Surgical Concerns Medistra Medical Journal (MMJ)
Vincentia Ade Rizky
INKES MEDISTRA LUBUK PAKAM
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IDENTIFIKASI BAKTERI ESCHERICHIA COLI O157:H7 PADA FESES PENDERITA DIARE DENGAN METODE KULTUR DAN PCR Rizky, Vincentia Ade; Siregar, Sa’adah; Krisdianilo, Visensius; Rahayu, Asvia; Syafrina Ginting, Suventi; ., Kartini
Jurnal FARMASIMED (JFM) Vol 3 No 2 (2021): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i2.615

Abstract

Escherichia coli O157: H7 is the main cause of foodborne disease in several countries, one of which is diarrhea. Diarrheal disease is still a major problem in Indonesia that needs treatment and study from various aspects. The conventional method of laboratory examination such as culture is a method that is often carried out, but in making the diagnosis requires a long time, the number of samples is large, and the results are less accurate because contamination can occur. Another more accurate technique for detecting Escherichia coli O157: H7 is the PCR technique. This study aims to identify the Escherichia coli O157: H7 bacteria by culture method and PCR. The results showed that the culture method and PCR of 8 isolated samples 4 showed positive results for the bacterium Escherichia coli O157: H7. However, the PCR method is more selective and faster than the culture method.
KARAKTERISASI DAN IDENTIFIKASI MOLEKULER BAKTERI PENGHASIL ENZIM PROTEASE DARI TEMPE YANG DIPERJUALBELIKAN DI PASAR LUBUK PAKAM Krisdianilo, Visensius; Siregar, Sa'adah; Rizky, Vincentia Ade
Jurnal FARMASIMED (JFM) Vol 4 No 1 (2021): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v4i1.616

Abstract

Protease enzyme is an enzyme that is important in protein breakdown. Animals, plants as well as microorganisms such as bacteria can produce this protease enzyme. In its application protease enzymes can be used in the pharmaceutical industry, detergent industry, skin products as well as food products. Tempe is one of the traditional food products that have been known for a long time, tempeh is made from soybean seeds fermented by mushrooms. Molecular identification can use polymerase chain reaction (PCR) method, PCR is the process of multiplying a certain nucleotide sequence using enzymatic processes in vitro. The presence of protein content in tempeh can be possible the presence of bacteria that can break down proteins in the tempeh, especially tempeh that has been fermented about 48-72 hours. Based on the results of characterization and identification of 5 isolates of tempeh post-fermentation 72 hours, positive results of protease enzymes found in isolate TPLP-1, TPLP-2 and TPLP-5, with the largest zone diameter in isolate TPLP-2 50 mm, then isolate with the highest protease enzyme activity isolate TPLP-2 molecularly identified by identifying the gene 16S rRNA which is subsequently included in the BLAST program and obtained by isolate TPLP-2 identified as Pseudomons stuastzeri.
EVALUASI PENGGUNAAN OBAT ASAM URAT DAN POLA PERESEPANNYA PADA PASIEN GOUT ARTRITIS DI INSTALASI RAWAT INAP DI RSUD DELI SERDANG LUBUK PAKAM TAHUN 2020 Rahayu, Asvia; Anna Teresia Marbun, Romauli; Nopita Sari Manalu, Dian; Siregar, Sa’adah; Ade Rizky, Vincentia; Krisdianilo, Visensius
Jurnal FARMASIMED (JFM) Vol 3 No 2 (2021): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i2.681

Abstract

Uric acid is a disease that arises due to excess purine in the body caused by uric crystals in the joint tissue that attacks the fingers, elbows, knees, toes, toes and heels. Gouty arthritis is a progressive disease caused by deposition of monosodium urate crystals (MSU) found in the joints, kidneys, and other connective tissue due to chronic hyperuricemia. The incidence of gouty arthritis is common in men as many as 25 patients (65.8%) and women as many as 13 patients (34.2%). The incidence of gouty arthritis continues to increase with age, at the age of 21-35 years (7.9%), ages 36-55 years (36.7%), age 56-65 years (28.9%), and at the age of 66 -75 years (26.3%). The purpose of this study was to examine the use of uric acid and its prescription patterns in arthritis patients in Deli Serdang Lubuk Pakam Hospital in 2020 in terms of the right parameters according to the indications, the right patients, the right drugs, and the right dosages. This research is a non-experimental study, data retrospective retrieval was done by looking at the medical record data of arthritis gout patients and analyzed descriptively. Sampling as many as 38 patients with puposive sampling method. Inclusion criteria in this study were patients who were diagnosed with gout and given arthritis medication with complete medical record data. The results of the study evaluated the use of gout and its prescribing patterns in 38 patients at Deli Serdang Lubuk Pakam Regional Hospital in 2020, which were 100% correct, 100% right, 100% right, and 96% right.
EVALUATION OF TITER RESULTS ON WIDAL EXAMINATION BASED ON THE DURATION OF FEVER IN HOSPITAL GRANDMED LUBUK PAKAM Rahayu, Asvia; Krisdianilo, Visensius; Hutabarat, Sintya; Siregar, S'aadah; Rizky, Vincentia Ade
Jurnal FARMASIMED (JFM) Vol 4 No 2 (2022): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v4i2.1010

Abstract

Kultur merupakan salah satu metode yang digunakan untuk diagnosis demam tifoid, namun di beberapa daerah seringkali tidak ada fasilitas untuk kultur, maka cara lain untuk membantu menegakkan diagnosis yang praktis dan tersedia di rumah sakit adalah dengan tes Widal. Tujuan penelitian ini adalah untuk mengetahui evaluasi hasil titer pada pemeriksaan Widal berdasarkan lama demam di RS GrandMed Lubuk Pakam. Penelitian ini dilakukan di Laboratorium Patologi Klinik RS GrandMed Lubuk Pakam. Jenis penelitian ini adalah deskriptif yang dilaksanakan mulai bulan Mei 2021 sampai dengan Juni 2021. Jumlah sampel yang diperoleh sebanyak 25 orang. Dari 25 sampel tersebut, hasil uji Widal dengan titer antibodi terhadap antigen O 1:80 adalah 2 orang, 1:160 orang, 1:320 orang, 2 orang. Titer antibodi terhadap antigen H 1:80 sebanyak 2 orang, 1:160 sebanyak 4 orang, 1:320 sebanyak 2 orang, dan titer antibodi terhadap antigen AH 1:80 sebanyak 6 orang, 1:160 sebanyak 5 orang dan 1:320 sebanyak 1 orang. Kesimpulan dari penelitian ini adalah 1:160 merupakan titer paling banyak ditemukan dengan titer antibodi tertinggi terhadap antigen H yaitu 1:320 lebih sering ditemukan pada durasi demam dengan kisaran 6-9 hari sedangkan titer antibodi tertinggi terhadap antigen AH adalah 1:320 ditemukan selama penelitian. demam dengan kisaran 6-9 hari.
PERBEDAAN MODIFIKASI LARUTAN ZnS (Zinc sulfite) DENGAN NaCl (Natrium Cloride) 0.9 % METODE FLOTASI TERHADAP JUMLAH TELUR CACING Soil Transmitted Helminth (STH) Siregar, Saadah; Manurung, Arnita Elisabet; Rizky, Vincentia Ade; Krisdianilo, Vinsensius
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1405

Abstract

Worms is a disease caused by the entry of parasites in the form of worms into the human body due to the influence of environmental factors or food that is not kept clean. Factors that cause the risk of helminthiasis in humans include food that is infected with worm eggs, feet that are in direct contact with soil containing worm vectors because they do not wear footwear, the habit of defecating in random places, availability of clean water, hand washing, washing habits. plates, And nail hygiene. The purpose of this study was to determine the difference in the number of worm eggs to be found using ZnS 1% and NaCl 0.9% solution using the flotation method. The data obtained from faecal examination using the flotation method with ZnS 1% and NaCl 0.9%, namely Soil Transmitted Helminth (STH) worm eggs, then the number of eggs will be presented in table form and analyzed descriptively. Result With the average yield of worm eggs found in  a solution of ZnS  1% and NaCl 0.9% using the flotation method for the number of Soil Transmitted Helminth (STH) worm eggs. In ZnS 1% solution the average is 2.12 with 4 Ascaris lumbricoides eggs, Trichuris trichura 2 and 3 hookworms while in 0.9% NaCl solution the average is 1.31 with 5 Ascaris lumbricoides worm eggs, Trichuris trichura 1 and worms mine 1. The Manny Whitney statistical test showed results of Sig 0.003 (P<0.005), so it was concluded that there was a difference in the number of STH worm eggs found using ZnS 1% solution and 0.9% NaCl using the flotation method.
Perbandingan Pertumbuhan Aspergillus flavus Pada Media Filtrat Bekatul Dengan Berbagai Variasi Konsentrasi Sa’adah Siregar; Vincentia Ade Rizky; Syukurin Juliani Gulo
SEHATI: Jurnal Kesehatan Vol 5, No 1 (2025)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/sehati.v5i1.79

Abstract

Mushrooms play a lot of role in life, both beneficial and detrimental. Certain types of fungi are capable of producing a toxic organic compound called mycotoxin. The mycotoxin produced by Aspergillus flavus is known as aflatoxin. The mushroom growth medium that is often used is SDA (Sabouraud Dextrose Agar). The composition of SDA media is peptone mycology, sugar, agar. The content of rice bran is carbohydrates, protein, water, and agar. The purpose of the study was to determine the comparison of growth diameter and effectiveness of Aspergillus flavus  concentration on bran filtrate media with various concentration variations. The method used in this study was experimental with inoculation of Aspergillus flavus fungi with a single dot method. The average colony diameter concentrations of 5%, 10%, 15% and 20% were 5.3 mm, 8.4 mm, 11.4 mm, and 16.5 mm. On SDA media  the average colony diameter is 17.6 mm. The concentration of bran filtrate media that is close to SDA  media is a concentration of 20%.  The results  of the One Way ANOVA  test showed a significant difference in the growth of Aspergillus flavus between various variations in bran filtrate concentration obtained a sig value of 0.000 less than 0.05. The conclusion of  data analysis using the One Way ANOVA  test was a significant difference in the growth of Aspergillus flavus between various variations in bran filtrate concentration obtained a sig value of 0.000 less than 0.05. In the bonfferoni test results, it was found that a concentration of 20% had similarities or significance to the positive control media of natural resources with a value of (p) 0.331 more than 0.05 which means that there is no significant difference between bran filtrate media at a concentration of 20% with positive control media  of natural resources
SOSIALISASI PENINGKATAN DETEKSI SOIL TRANSMITTED HELMINTH (STH) MELALUI MODIFIKASI PADA LARUTAN ZnS (SULFIDA SENG) DAN NaCl (NATRIUM KLORIDA) 0,9% Siregar, Sa’adah; Rizky, Vincentia Ade
EJOIN : Jurnal Pengabdian Masyarakat Vol. 2 No. 6 (2024): EJOIN : Jurnal Pengabdian Masyarakat, Juni 2024
Publisher : LPPM Institut Pendidikan Nusantara Global

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/ejoin.v2i6.2998

Abstract

Public health is a crucial aspect of disease prevention efforts. One significant health issue in many countries, including Indonesia, is Soil Transmitted Helminth (STH) infection, often caused by poor sanitation and lack of environmental cleanliness awareness. STH, typically caused by Ascaris lumbricoides, Trichuris trichiura, and hookworm, can have severe health impacts, particularly on children. Fecal examination methods, especially flotation techniques using ZnS (zinc sulfide) and 0.9% NaCl (sodium chloride) solutions, are commonly employed for STH detection. However, optimizing flotation methods in the field is essential to enhance STH detection sensitivity. This study focuses on the modification of ZnS and 0.9% NaCl solutions to improve the effectiveness of detecting STH eggs. By disseminating information on these modifications to public health practitioners, this socialization aims to enhance detection and prevention efforts for STH infections. The evaluation results show significant improvements in participants' knowledge, skills, attitudes, understanding, method application, and satisfaction, indicating the effectiveness of this socialization in promoting better STH control and prevention programs.
PENGARUH KONSENTRASI MEDIA KACANG KEDELAI(Glycine max L. Merr) DALAM PERTUMBUHAN BAKTERI Staphylococcus aureus  DIBANDINGKAN DENGAN  NUTRIENT Siregar, Sa’adah; Rizky, Vincentia Ade; Nasution, Salmiah
EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan Vol. 2 No. 2 (2025): EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan, Juni 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/empiris.v2i2.1285

Abstract

Soybean is a complete protein, inexpensive and is one of the food ingredients that contain groups of essential and non-essential amino acids, carbohydrates, fats, vitamins and minerals. Staphylococcus is coccus bacteria, gram positive with an irregular arrangement or clusters like grapes. Staphylococcus aureus is a bacterium that is non-spore, non-motile, facultative anaerobe, oxidase negative and catalase positive. The aim of the study was to determine the growth of Staphylococcus aureus bacteria in alternative media for soybeans as a substance for Nutrient agar media. The type of experimental research is to conduct a trial on the concentration of soy bean powder. research  population of soybeans purchased at Lubuk Pakam market. Is an experimental method for the growth of staphylococcus aureus bacteria in soybean media as a substitute for Nutrient agar. Of research from soybeans (Glycine max L. Merr) can be used as an alternative source of protein in the manufacture of alternative media, but have slight differences in the size of the colony, the color of the colony and the color of the pigment produced with varying concentrations of 20%, 40%, 60% and 80%.  Soybeans are effectively used as an alternative protein source to replace Nutrient agar media in growing Staphylococcus aureus bacteria for a more effective concentration, namely at a concentration of 60% when viewed from the number of colonies obtained in the soybean media
Pewarnaan alternatif alami daun miana (Coleus scutellarioides (L) benth) sebagai pengganti gentian violet pada pewarnaan gram bakteri staphylococcus aureus RIZKY, VINCENTIA ADE; SIREGAR, SAADAH; KRISDIANILO, VISENSIUS; RISKA WAHYUNI
Medistra Medical Journal (MMJ) Vol 1 No 2 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v1i2.2095

Abstract

Daun miana (Coleus scutellarioides (L) benth) yang memiliki corak ungu kemerahan mengindifikasikan terdapat antosianin, salah satu variannya yaitu crispa. Penelitian ini bertujuan untuk menguji zat warna antosianin pada sari daun miana sebagai pengganti gentian violet pada pewarnaan gram, Jenis penelitian yang digunakan adalah eksperimental/eksperimen. penelitian ini bertujuan untuk mengetahui apakah sari daun miana (Coleus scutellarioides (L) benth) mampu sebagai bahan alternatif pengganti reagen gentian violet pada bakteri Staphylococcus aureus. Dan pada penelitian ini dilakukan perasan sari daun miana (Coleus scutellarioides (L) benth). sampel bakteri Staphylococcus aureus dibuat sediaan preparat dan pewarnaan menggunakan gentian violet sebagai bahan kontrol dan menggunakan sari daun miana (Coleus scutellarioides (L) benth) sebagai eksperimen menggunakan konsentrasi sari daun miana yaitu 1%, 0,1% dan juga 0,5%. Data yang diperoleh diolah mengggunakan analisa data deskriftif. Hasil penelitian pada sari daun miana sari konsentrasi 1%, dan 0,1% mampu mewarnai bakteri Staphylococcus aureus dibandingan dengan konsentrasi 0,5%. Penelitian ini dapat juga dikembangkan dengan uji coba pada daun lain yang memiliki kandungan antosianin dan perlu juga melalukan konsentrasi yang lebih tinggi atau pun menggunakan metode yang lain.
Utilization of Lemon Juice (Citrus limon L.) as an Alternative Substitute for Sodium Citrate in Blood Sedimentation Rate Examination RIZKY, VINCENTIA ADE; PUTRI, IKKE ELMALIA; SIREGAR, SAADAH
Medistra Medical Journal (MMJ) Vol 2 No 1 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i1.2370

Abstract

Background: The erythrocyte sedimentation rate (LED) examination is a hematological test that describes the rate at which red blood cells settle in plasma, using 3.8% sodium citrate anticoagulant and is measured in mm/hour. Lemon (Citrus limon L.) is a plant with natural properties as a metal binding agent or chelating agent. Lemons contain vitamin C, citric acid, potassium, and limonene. Citric acid compounds are able to form complex bonds with metals because they have COOH and -OH functional groups. Apart from citric acid, lemons contain flavonoids which function as antiviral, anticancer and antiallergenic. Objective: To test whether lemon juice (Citrus limon L.) can be used as an alternative ingredient to replace sodium citrate in LED tests. Method: This research is an experiment using the Westergreen method. Results: LED examination with 3.8% sodium citrate produced a value of 10 mm/hour. Lemon juice with a concentration of 3.8% produces a value of 15 mm/hour, 10% produces a value of 10 mm/hour, 50% produces a value of 18 mm/hour, and 100% produces a value of 0 mm/hour. Conclusion: A lemon juice concentration of 10% showed results closest to the control, with similar LED values. Thus, lemon juice (Citrus limon L.) can serve as a substitute for sodium citrate in the LED test
Co-Authors Adipura Atmadja Egok Akmal, Dzul Anna Teresia Marbun, Romauli Asvia Rahayu Asvia Rahayu Asvia Rahayu Avinda Sari Kanthi Rahayu Awaluddin DALIMUNTHE, HAFIKA Dian Pratiwi Eka Nurul H.P.S Hutabarat, Sintya Indriani Indriani Jayanti, Neti Eka Kartini . Krisdianilo, Vinsensius Krisdianilo, Visensius Lemriani Manullang, Selda Manurung, Arnita Elisabet Maria Selviana Joni Morischa Deyla Sitanggang Nasrullah Tamrin Nasution, Salmiah Neti Eka Jayanti Ni’mah Hidayatul Laili Nopita Sari Manalu, Dian PUTRI, IKKE ELMALIA Rachmat Saleh Rahayu, Asvia Ramadhani, Siti Riska Wahyuni Sa'adah Siregar SAADAH SIREGAR Saadah Siregar, Saadah Sa’adah Siregar Siregar, S'aadah Siregar, Sa'adah Siregar, Sa’adah Siregar, Sa’adah Sitanggang, Morischa Deyla Sri Mulyani Suci Mustika Mawardani Sutriyawan, Agung Syafrina Ginting, Suventi Syukurin Juliani Gulo Tri Astuti Sugiyatmi Umi Latifah Visensius Krisdianilo Visensius Krisdianilo Visensius Krisdianilo Visensius Krisdianilo, Visensius Wenda Putri Natal Saragih