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Perbedaan Teknik Penanaman Terhadap Hasil Jumlah Koloni Bakteri Escherichia Coli Pada Suhu Inkubasi 36C: Perbedaan Teknik Penanaman Terhadap Hasil Jumlah Koloni Bakteri Escherichia Coli Pada Suhu Inkubasi 36C RIZKY, VINCENTIA ADE
Jurnal FARMASIMED (JFM) Vol 1 No 2 (2019): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.942 KB) | DOI: 10.35451/jfm.v1i2.173

Abstract

Escherichia coli bacteria are gram-negative bacteria in form of single or paired cells it is included Enterobacteriaceae family and intestinal normal flora. Laboratory tests conducted for the calculation of the number of germs can be done using cultivation techniques using a loop and micropipet. The aim of this research is to know the difference of number of bacteria bacteria on calibrated loop and micropipet to colony of Escherichia coli bacteria. This research is an analytical observation study with cross sectional design. The hypothesis was tested using independent t test with 95% confidence level. The results showed the average number colonies of Escherichia coli bacteria growing on PCA media using calibrated loop technique was 138,25 CFU/mL where as the average number of colonies of Escherichia coli bacteria grown on PCA media using micropipet technique was 104,56 CFU/mL, and significant value of p = 0,001. Furthemore, the result of data analysis showed that there were no any differences in the number of colonies of Escherichia coli bacteria in planting using calibrated loop technique and micropipet at incubation temperature of 36ºC.
EFEKTIFITAS PENGGUNAAN PEWARNA ALTERNATIF PREPARAT PERMANEN TELUR NEMATODA USUS MENGGUNAKAN PEWARNA RHODAMIN B Siregar, Saadah; Krisdianilo, Visensius; Rizky, Vincentia Ade
Jurnal FARMASIMED (JFM) Vol 2 No 1 (2019): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.612 KB) | DOI: 10.35451/jfm.v2i1.194

Abstract

Staining is one of support microscopic examination in the identification of worm eggs. Staining techniques are needed to clarify the various elements and the description of the microscopic worm eggs and distinguish it from the surrounding dirt. Hematoxylin and Eosin staining method that is widely used in the staining of histological tissue. Dyes commonly used in the examination of intestinal nematode eggs on is dye eosin. To added reference dye Rhodamine B dye used as an alternative to dye eosin. The method used is experiment by conducting concentration ratio of Rhodamine B 1%, 1.5%, 2% and 2.5% to 2% eosin control. The results based on Kruskal-Wallis Test showed the normality test is 0.357, p-value sig (0.357) > 0.05, so that the data group to be tested otherwise normal. Then performed statistical tests Kruskal WallisTtest to determine the accuracy of the file thus obtained p-value (0.407) > 0.05, meaning there is no significant difference in the concentration of the dye Rhodamine B of the entire treatment. Morphology of worm eggs clearly visible from the outside layer to the inside layer, the visual field Rhodamine B dye is clearly visible, color contrast eggs are also clearly visible. From the research conducted, it can be concluded that the dye Rhodamine B dye can be used as an alternative to dye eosin.
PERBANDINGAN AKTIVITAS ANTIBAKTERI INFUSA DAUN JERUK NIPIS (CITRUS AURANTIFOLIA) DAN DAUN JERUK PURUT (CITRUS HYSTRIX) TERHADAP BAKTERI ESCHERICHIA COLI Siregar, Saadah; Indriani, Indriani; Vincentia Ade Rizky, Vincentia; Visensius Krisdianilo, Visensius; Anna Teresia Marbun, Romauli
Jurnal FARMASIMED (JFM) Vol 3 No 1 (2020): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i1.524

Abstract

Plant Lime (Citrus aurantifolia) and Plant Kaffir Lime (Citrus hystrix) including Rutacea family. Part of the plant lemon and lime are used as a drug other than fruit and leaves that can be used as medicine. This study aims to determine the antibacterial activity infuse lime leaves and lime leaves. Examination of the characteristics simplicia macroscopic examination. Phytochemical screening of compounds includes examining alkaloids, flavonoids, tannins and infuse saponin. Ekstrak conducted using distilled water solvent. Test of antibacterial activity against Escherichia coli bacteria carried by the agar diffusion method using a paper disc. Results obtained from macroscopic examination for lime leaves are fresh leaves leaf-shaped single smooth surface and the lower surface of the leaves are light green, the dark green upper surface. If torn, lime leaves produce coarse fibers. The leaves are small with a width of 3-5 cm. For lime leaves are fresh leaves, leaf litter pinnate compound leaves one. Child leaf blade oval to oblong, base rounded or blunt, the blunt end up pointed, 8-15 cm long, 2-6 cm wide, the upper surface colour some what shiny dark green, light green below the surface. Results of phytochemical screening simplicia powder lime leaves and lime leaves that contain a class of alkaloids, flavonoids and tannins. Antibacterial activity test results showed that there were differences between the antibacterial infusion lime leaves and lime leaves kaffir lime leaves which are more effective against the bacteria Escherichia coli compared kaffir lime leaves.
IDENTIFIKASI BAKTERI ESCHERICHIA COLI O157:H7 PADA FESES PENDERITA DIARE DENGAN METODE KULTUR DAN PCR Rizky, Vincentia Ade; Siregar, Sa’adah; Krisdianilo, Visensius; Rahayu, Asvia; Syafrina Ginting, Suventi; ., Kartini
Jurnal FARMASIMED (JFM) Vol 3 No 2 (2021): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i2.615

Abstract

Escherichia coli O157: H7 is the main cause of foodborne disease in several countries, one of which is diarrhea. Diarrheal disease is still a major problem in Indonesia that needs treatment and study from various aspects. The conventional method of laboratory examination such as culture is a method that is often carried out, but in making the diagnosis requires a long time, the number of samples is large, and the results are less accurate because contamination can occur. Another more accurate technique for detecting Escherichia coli O157: H7 is the PCR technique. This study aims to identify the Escherichia coli O157: H7 bacteria by culture method and PCR. The results showed that the culture method and PCR of 8 isolated samples 4 showed positive results for the bacterium Escherichia coli O157: H7. However, the PCR method is more selective and faster than the culture method.
KARAKTERISASI DAN IDENTIFIKASI MOLEKULER BAKTERI PENGHASIL ENZIM PROTEASE DARI TEMPE YANG DIPERJUALBELIKAN DI PASAR LUBUK PAKAM Krisdianilo, Visensius; Siregar, Sa'adah; Rizky, Vincentia Ade
Jurnal FARMASIMED (JFM) Vol 4 No 1 (2021): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v4i1.616

Abstract

Protease enzyme is an enzyme that is important in protein breakdown. Animals, plants as well as microorganisms such as bacteria can produce this protease enzyme. In its application protease enzymes can be used in the pharmaceutical industry, detergent industry, skin products as well as food products. Tempe is one of the traditional food products that have been known for a long time, tempeh is made from soybean seeds fermented by mushrooms. Molecular identification can use polymerase chain reaction (PCR) method, PCR is the process of multiplying a certain nucleotide sequence using enzymatic processes in vitro. The presence of protein content in tempeh can be possible the presence of bacteria that can break down proteins in the tempeh, especially tempeh that has been fermented about 48-72 hours. Based on the results of characterization and identification of 5 isolates of tempeh post-fermentation 72 hours, positive results of protease enzymes found in isolate TPLP-1, TPLP-2 and TPLP-5, with the largest zone diameter in isolate TPLP-2 50 mm, then isolate with the highest protease enzyme activity isolate TPLP-2 molecularly identified by identifying the gene 16S rRNA which is subsequently included in the BLAST program and obtained by isolate TPLP-2 identified as Pseudomons stuastzeri.
EVALUASI PENGGUNAAN OBAT ASAM URAT DAN POLA PERESEPANNYA PADA PASIEN GOUT ARTRITIS DI INSTALASI RAWAT INAP DI RSUD DELI SERDANG LUBUK PAKAM TAHUN 2020 Rahayu, Asvia; Anna Teresia Marbun, Romauli; Nopita Sari Manalu, Dian; Siregar, Sa’adah; Ade Rizky, Vincentia; Krisdianilo, Visensius
Jurnal FARMASIMED (JFM) Vol 3 No 2 (2021): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i2.681

Abstract

Uric acid is a disease that arises due to excess purine in the body caused by uric crystals in the joint tissue that attacks the fingers, elbows, knees, toes, toes and heels. Gouty arthritis is a progressive disease caused by deposition of monosodium urate crystals (MSU) found in the joints, kidneys, and other connective tissue due to chronic hyperuricemia. The incidence of gouty arthritis is common in men as many as 25 patients (65.8%) and women as many as 13 patients (34.2%). The incidence of gouty arthritis continues to increase with age, at the age of 21-35 years (7.9%), ages 36-55 years (36.7%), age 56-65 years (28.9%), and at the age of 66 -75 years (26.3%). The purpose of this study was to examine the use of uric acid and its prescription patterns in arthritis patients in Deli Serdang Lubuk Pakam Hospital in 2020 in terms of the right parameters according to the indications, the right patients, the right drugs, and the right dosages. This research is a non-experimental study, data retrospective retrieval was done by looking at the medical record data of arthritis gout patients and analyzed descriptively. Sampling as many as 38 patients with puposive sampling method. Inclusion criteria in this study were patients who were diagnosed with gout and given arthritis medication with complete medical record data. The results of the study evaluated the use of gout and its prescribing patterns in 38 patients at Deli Serdang Lubuk Pakam Regional Hospital in 2020, which were 100% correct, 100% right, 100% right, and 96% right.
EVALUATION OF TITER RESULTS ON WIDAL EXAMINATION BASED ON THE DURATION OF FEVER IN HOSPITAL GRANDMED LUBUK PAKAM Rahayu, Asvia; Krisdianilo, Visensius; Hutabarat, Sintya; Siregar, S'aadah; Rizky, Vincentia Ade
Jurnal FARMASIMED (JFM) Vol 4 No 2 (2022): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v4i2.1010

Abstract

Kultur merupakan salah satu metode yang digunakan untuk diagnosis demam tifoid, namun di beberapa daerah seringkali tidak ada fasilitas untuk kultur, maka cara lain untuk membantu menegakkan diagnosis yang praktis dan tersedia di rumah sakit adalah dengan tes Widal. Tujuan penelitian ini adalah untuk mengetahui evaluasi hasil titer pada pemeriksaan Widal berdasarkan lama demam di RS GrandMed Lubuk Pakam. Penelitian ini dilakukan di Laboratorium Patologi Klinik RS GrandMed Lubuk Pakam. Jenis penelitian ini adalah deskriptif yang dilaksanakan mulai bulan Mei 2021 sampai dengan Juni 2021. Jumlah sampel yang diperoleh sebanyak 25 orang. Dari 25 sampel tersebut, hasil uji Widal dengan titer antibodi terhadap antigen O 1:80 adalah 2 orang, 1:160 orang, 1:320 orang, 2 orang. Titer antibodi terhadap antigen H 1:80 sebanyak 2 orang, 1:160 sebanyak 4 orang, 1:320 sebanyak 2 orang, dan titer antibodi terhadap antigen AH 1:80 sebanyak 6 orang, 1:160 sebanyak 5 orang dan 1:320 sebanyak 1 orang. Kesimpulan dari penelitian ini adalah 1:160 merupakan titer paling banyak ditemukan dengan titer antibodi tertinggi terhadap antigen H yaitu 1:320 lebih sering ditemukan pada durasi demam dengan kisaran 6-9 hari sedangkan titer antibodi tertinggi terhadap antigen AH adalah 1:320 ditemukan selama penelitian. demam dengan kisaran 6-9 hari.
PERBEDAAN MODIFIKASI LARUTAN ZnS (Zinc sulfite) DENGAN NaCl (Natrium Cloride) 0.9 % METODE FLOTASI TERHADAP JUMLAH TELUR CACING Soil Transmitted Helminth (STH) Siregar, Saadah; Manurung, Arnita Elisabet; Rizky, Vincentia Ade; Krisdianilo, Vinsensius
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1405

Abstract

Worms is a disease caused by the entry of parasites in the form of worms into the human body due to the influence of environmental factors or food that is not kept clean. Factors that cause the risk of helminthiasis in humans include food that is infected with worm eggs, feet that are in direct contact with soil containing worm vectors because they do not wear footwear, the habit of defecating in random places, availability of clean water, hand washing, washing habits. plates, And nail hygiene. The purpose of this study was to determine the difference in the number of worm eggs to be found using ZnS 1% and NaCl 0.9% solution using the flotation method. The data obtained from faecal examination using the flotation method with ZnS 1% and NaCl 0.9%, namely Soil Transmitted Helminth (STH) worm eggs, then the number of eggs will be presented in table form and analyzed descriptively. Result With the average yield of worm eggs found in  a solution of ZnS  1% and NaCl 0.9% using the flotation method for the number of Soil Transmitted Helminth (STH) worm eggs. In ZnS 1% solution the average is 2.12 with 4 Ascaris lumbricoides eggs, Trichuris trichura 2 and 3 hookworms while in 0.9% NaCl solution the average is 1.31 with 5 Ascaris lumbricoides worm eggs, Trichuris trichura 1 and worms mine 1. The Manny Whitney statistical test showed results of Sig 0.003 (P<0.005), so it was concluded that there was a difference in the number of STH worm eggs found using ZnS 1% solution and 0.9% NaCl using the flotation method.
Perbandingan Pertumbuhan Aspergillus flavus Pada Media Filtrat Bekatul Dengan Berbagai Variasi Konsentrasi Sa’adah Siregar; Vincentia Ade Rizky; Syukurin Juliani Gulo
SEHATI: Jurnal Kesehatan Vol 5, No 1 (2025)
Publisher : Pelantar Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52364/sehati.v5i1.79

Abstract

Mushrooms play a lot of role in life, both beneficial and detrimental. Certain types of fungi are capable of producing a toxic organic compound called mycotoxin. The mycotoxin produced by Aspergillus flavus is known as aflatoxin. The mushroom growth medium that is often used is SDA (Sabouraud Dextrose Agar). The composition of SDA media is peptone mycology, sugar, agar. The content of rice bran is carbohydrates, protein, water, and agar. The purpose of the study was to determine the comparison of growth diameter and effectiveness of Aspergillus flavus  concentration on bran filtrate media with various concentration variations. The method used in this study was experimental with inoculation of Aspergillus flavus fungi with a single dot method. The average colony diameter concentrations of 5%, 10%, 15% and 20% were 5.3 mm, 8.4 mm, 11.4 mm, and 16.5 mm. On SDA media  the average colony diameter is 17.6 mm. The concentration of bran filtrate media that is close to SDA  media is a concentration of 20%.  The results  of the One Way ANOVA  test showed a significant difference in the growth of Aspergillus flavus between various variations in bran filtrate concentration obtained a sig value of 0.000 less than 0.05. The conclusion of  data analysis using the One Way ANOVA  test was a significant difference in the growth of Aspergillus flavus between various variations in bran filtrate concentration obtained a sig value of 0.000 less than 0.05. In the bonfferoni test results, it was found that a concentration of 20% had similarities or significance to the positive control media of natural resources with a value of (p) 0.331 more than 0.05 which means that there is no significant difference between bran filtrate media at a concentration of 20% with positive control media  of natural resources
SOSIALISASI PENINGKATAN DETEKSI SOIL TRANSMITTED HELMINTH (STH) MELALUI MODIFIKASI PADA LARUTAN ZnS (SULFIDA SENG) DAN NaCl (NATRIUM KLORIDA) 0,9% Siregar, Sa’adah; Rizky, Vincentia Ade
EJOIN : Jurnal Pengabdian Masyarakat Vol. 2 No. 6 (2024): EJOIN : Jurnal Pengabdian Masyarakat, Juni 2024
Publisher : LPPM Institut Pendidikan Nusantara Global

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/ejoin.v2i6.2998

Abstract

Public health is a crucial aspect of disease prevention efforts. One significant health issue in many countries, including Indonesia, is Soil Transmitted Helminth (STH) infection, often caused by poor sanitation and lack of environmental cleanliness awareness. STH, typically caused by Ascaris lumbricoides, Trichuris trichiura, and hookworm, can have severe health impacts, particularly on children. Fecal examination methods, especially flotation techniques using ZnS (zinc sulfide) and 0.9% NaCl (sodium chloride) solutions, are commonly employed for STH detection. However, optimizing flotation methods in the field is essential to enhance STH detection sensitivity. This study focuses on the modification of ZnS and 0.9% NaCl solutions to improve the effectiveness of detecting STH eggs. By disseminating information on these modifications to public health practitioners, this socialization aims to enhance detection and prevention efforts for STH infections. The evaluation results show significant improvements in participants' knowledge, skills, attitudes, understanding, method application, and satisfaction, indicating the effectiveness of this socialization in promoting better STH control and prevention programs.