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All Journal BIOMA : Jurnal Biologi Makassar Jurnal Kesehatan Komunitas JURNAL FARMASIMED (JFM) Jurnal Ilmiah Kesehatan Rustida Journal of Nursing and Public Health (JNPH) Journal SAGO Gizi dan Kesehatan SEHATI: Jurnal Kesehatan Jurnal Riset Rumpun Matematika dan Ilmu Pengetahuan Alam (JURRIMIPA) Insight Power (Kekuatan Wawasan) - Jurnal Pengabdian Kepada Masyarakat Digital (JUPED) Termometer: Jurnal Ilmiah Ilmu Kesehatan Dan Kedokteran EJOIN : Jurnal Pengabdian Masyarakat Jurnal Pengmas Kestra (JPK) Jurnal Kabar Masyarakat Jurnal Laboratorium Khatulistiwa Indonesian Journal of Community Empowerment Jurnal Medika: Medika Jurnal Sains, Teknologi dan Kesehatan Science: Indonesian Journal of Science Journal of Medical Surgical Concerns Medistra Medical Journal (MMJ) Jurnal Ilmu Kedokteran dan Kesehatan Indonesia
Vincentia Ade Rizky
Universitas Borneo Tarakan
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Utilization of Kluwih Seed Starch (Artocarpus camansi) as an Alternative Growth Medium for Aspergillus niger Fungus saadah siregar; HAFIKA DALIMUNTHE; VINCENTIA ADE RIZKY
Medistra Medical Journal (MMJ) Vol 2 No 1 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i1.2372

Abstract

Background: Carbohydrates in kluwih seeds have sufficient nutrients, such as potatoes and tubers, making it possible to be used as a medium for mushroom growth. The nutritional composition of breadfruit contains 25% carbohydrates, 1.5% protein, and 0.3% fat. Aspergillus niger fungi require nutrients such as carbohydrates and protein to grow. This study aims to determine whether kluwih seed starch can be used as an alternative medium for the growth of Aspergillus niger fungi. Method: This study used a pre-experimental design with laboratory observation. The study was conducted by utilizing kluwih seed starch as a medium, compared to Potato Dextrose Agar (PDA) and Sabouraud Dextrose Agar (SDA). Results: The results showed that kluwih seed starch can be used as an alternative medium for the growth of Aspergillus niger, with fungal colonies that grow macroscopically and microscopically almost the same as PDA and SDA media. Conclusion: Kluwih seed starch can be an alternative substitute for PDA media for the growth of Aspergillus niger.
Modification Of Star Fruit Wuluh (Averrhoa Bilimbi L.) As A Substitute For Turk Solution In The Manual Method Leukocyte Count. Selda Lemriani Manullang; Vincentia Ade Rizky; Asvia Rahayu
Medistra Medical Journal (MMJ) Vol 2 No 2 (2025): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i2.2374

Abstract

Background: This study counts the number of leukocytes using a manual method. The natural materials used are lime, lemon, and tamarind, which contain glacial acid and citric acid with a pH below 2.0. These acids can lyse blood cells, except for leukocytes (white blood cells). Objective: This study aims to analyze the effectiveness of varying concentrations of starfruit (Averrhoa bilimbi) on the number of leukocytes (white blood cells). Method: This research employs an experimental method. The number of leukocytes is counted from the juice of starfruit at concentrations of 5% and 10%. The objective is to find the most effective concentration compared to the standard Turk solution as a control. Conclusion: The data were analyzed using One-Way ANOVA. The average number of leukocytes in the control group was 6,500, in the 10% concentration was 6,450, and in the 5% concentration was 4,500. The results of statistical tests and hypotheses showed a significance value of 0.000, which is less than ? (alpha) (0.05), thus the null hypothesis is rejected and there is a significant difference. The 10% concentration proved to be the most effective in replacing glacial acetic acid in the Turk solution.
Comparison Of Potato (Solanum tuerosum L) and Corn (Zea mays) as Alternative Media To Nutrient Agar Media In The Groth of Staphylococcus aureus Bacteria Rahmawati; Sa’adah Siregar; Vincentia Ade Rizky
Medistra Medical Journal (MMJ) Vol 2 No 1 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i1.2376

Abstract

Background: Microbiology is the study of microorganisms, which are microscopic organisms that can only be observed through a microscope. These microorganisms have an important role in various aspects of life, including in the health sector. Objective: This study aims to evaluate whether alternative media, such as potato and corn, can be used as a substitute for Nutrient Agar in supporting the growth of Staphylococcus aureus bacteria. Methods: This research is experimental, by comparing the effectiveness of potato and corn media as an alternative to Nutrient Agar for the growth of Staphylococcus aureus. Results: The results of the research in the One-way Anova test obtained a significant value (0.00) which means (p <0.05), so it can be concluded that there is a significant difference between the ability of Nutrient Agar media, potato media and corn media in growing Staphylococcus aureus bacteria. To find out each group that has a significant difference, the Post-hoc follow-up test is carried out. The results of the post-hoc follow-up test shown in Figure 4.2.2 obtained a significant value between Nutrient Agar media and potato media (0.00), Nutrient Agar media with corn media (0.558), and between potato and corn media (0.00). Conclusion: The results showed that potato media was significantly different in its ability to grow Staphylococcus aureus compared to Nutrient Agar and corn. Potato media shows potential as an alternative to Nutrient Agar, despite its different effectiveness.
PENYUSUNAN REKOMENDASI KESEHATAN LINGKUNGAN WILAYAH PESISIR BERBASIS DATA SEKUNDER Maria Selviana Joni; Vincentia Ade Rizky; Umi Latifah
MAJU : Indonesian Journal of Community Empowerment Vol. 3 No. 1 (2026): MAJU : Indonesian Journal of Community Empowerment, January 2026
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/maju.v3i1.2276

Abstract

Coastal areas are vulnerable to environmental health problems, particularly those related to sanitation, clean water access, and domestic waste management. Poor environmental conditions contribute to the increased risk of environment-based diseases such as diarrhea and skin infections. This community service activity aimed to develop environmental health recommendations for coastal areas based on secondary data analysis. The method used was descriptive analysis of secondary data obtained from health profiles, statistical reports, and regional development planning documents. The analysis showed gaps between existing environmental health conditions and ideal health standards. Based on these findings, environmental health intervention recommendations focusing on disease prevention, health education, and strengthening primary health services were formulated. These recommendations are expected to support stakeholders in planning sustainable environmental health programs in coastal areas.
Real time polymerase chain reaction assay (RT-PCR) sebagai tes cepat mycobacterium tuberculosis dari sampel dahak pasien tuberculosis Visensius Krisdianilo; Vincentia Ade Rizky; Sa’adah Siregar; Asvia Rahayu
JOURNAL OF Medical Surgical Concerns Vol. 4 No. 1 (2024): June Edition 2024
Publisher : Published by: Indonesian Public Health-Observer Information Forum (IPHORR) Kerjasama dengan Himpunan Perawat Medikal Bedah Indonesia (HIPMEBI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56922/msc.v4i1.371

Abstract

Background: Mycobacterium tuberculosis is known as a highly pathogenic bacterium. These bacteria are aerobic in shape like rods and have resistance to acids. This bacteria can cause tuberculosis (TB). TB disease has been widely recognized as a cause of death which is quite high in the world. Tuberculosis causes the death of nearly one million women each year. Currently, no country in the world is free from tuberculosis. Data shows that Indonesia is the third largest contributor of tuberculosis cases in the world. Examination of mycobacterium tuberculosis bacteria which is routinely carried out in hospitals or health centers uses microscopic diagnosis of acid-resistant bacilli (BTA). Recently, a rapid test of Mycobacterium tuberculosis was carried out using the semi-quantitative Real Time Polymerase Chain Reaction Assay (RT-PCR) method which targets the rpoB gene in Mycobacterium tuberculosis, which can automatically process preparations by extracting doxyribo nucleic acid (DNA) in a cartridge. The time needed for this diagnosis is approximately two hours until the results come out. Purpose: To see the results of the rapid test compared to the microscopic diagnosis at the Puskesmas. Method: Using the cross-sectional method, with a sample of 25 patients at the Lubuk Pakam Health Center. To detect Mycobacterium tuberculosis using the GeneXpert MTB/RIF tool. Furthermore, the data obtained was processed and examined using the Wilcoxon statistical test. Results: The value of the statistical test results using the Wilcoxon test showed that the p-value (Sig) was 0.264, the value was > 0.05, which means that in this study there were no significant differences in results between the microscopic method and RT-PCR. Conclusion: Overall there was no significant difference in the results between microscopy and RT-PCR methods. Keywords: Tuberculosis; Mycobacterium Tuberculosis; RT-PCR Pendahuluan: Mycobacterium tuberculosis dikenal sebagai bakteri yang sangat patogen. Bakteri ini berbentuk aerobik seperti batang dan memiliki ketahanan terhadap asam. Bakteri ini dapat menyebabkan tuberkulosis (TB). Penyakit TBC telah dikenal luas sebagai penyebab kematian yang cukup tinggi di dunia. Tuberkulosis menyebabkan kematian hampir satu juta wanita setiap tahun. Saat ini tidak ada satu negara pun di dunia yang bebas dari tuberkulosis. Data menunjukkan bahwa Indonesia merupakan penyumbang kasus tuberkulosis terbesar ketiga di dunia. Pemeriksaan bakteri mycobacterium tuberculosis yang rutin dilakukan di rumah sakit atau puskesmas menggunakan diagnosis mikroskopis acid-resistant basil (BTA). Baru-baru ini telah dilakukan uji cepat Mycobacterium tuberculosis menggunakan metode semi kuantitatif Real Time Polymerase Chain Reaction Assay (RT-PCR) yang menargetkan gen rpoB pada Mycobacterium tuberculosis, yang secara otomatis dapat memproses preparat dengan ekstraksi asam nukleat doxyribo (DNA) pada catridge. Waktu yang dibutuhkan dalam diagnosis ini adalah kurang lebih dua jam sampai hasilnya keluar. Tujuan: Untuk melihat hasil rapid test tersebut dibandingkan dengan diagnosis mikroskopis pada Puskesmas. Metode: Menggunakan metode  cross sectional, dengan sampel yaitu pasien Puskesmas Lubuk Pakam sebanyak 25 orang. Untuk mendeteksi Mycobacterium tuberculosis menggunakan alat GeneXpert MTB/RIF. Selanjutnya data yang didapat di olah dan diperiksa dengan menggunakan uji statistic Wilcoxon. Hasil: Nilai hasil uji statistic menggunakan uji Wilcoxon didapatkan hasil p-value (Sig) adalah 0.264 nilai tersebut > dari 0.05 yang berarti pada penelitian ini hasilnya tidak terdapat perbedaan hasil yang signifikan antara metode mikroskopis dengan RT-PCR. Simpulan: Secara keseluruhan tidak ada perbedaan yang signifikan dalam hasil antara metode mikroskopis dan RT-PCR.  
Penerapan Perilaku Hidup Bersih dan Sehat pada Karyawan Embung Persemaian di Kota Tarakan Maria Selviana Joni; Vincentia Ade Rizky; Umi Latifah; Tri Astuti Sugiyatmi
Jurnal Kabar Masyarakat Vol. 4 No. 2 (2026): Mei: JURNAL KABAR MASYARAKAT
Publisher : Institut Teknologi dan Bisnis Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54066/jkb.v4i2.3804

Abstract

Clean and Healthy Living Behavior (PHBS) is a promotive and preventive effort that aims to improve the degree of public health through behavior change. Nursery reservoir employees have a high risk of exposure to the work environment in the form of dirty water, biological waste, and pathogenic microorganisms. This community service activity was carried out to increase the knowledge and practice of PHBS among employees of the nursery reservoir in Juata Tarakan based on national PHBS indicators. The implementation method includes counseling, demonstration, and evaluation using pre-test and post-test. The results showed a significant increase in employee knowledge, with an average pre-test score of 58.7 increasing to 78.3 in the post-test. The statistical test resulted in a value of p = 0.000 (p < 0.05), which confirms the effectiveness of PHBS educational interventions in improving workers' health knowledge and behavior. These findings provide practical implications that counseling-based education and demonstrations can be an effective strategy in encouraging the implementation of PHBS in risky work environments. Thus, this activity is expected to be able to support the creation of a healthier work environment, increase productivity, and become a model for community-based occupational health interventions.
Analisis Epidemiologi Spasio-Temporal Infeksi Dengue Tahun 2016–2025 di Tarakan Tri Astuti Sugiyatmi; Vincentia Ade Rizky; Maria Selviana Joni; Rachmat Saleh; Umi Latifah
Termometer: Jurnal Ilmiah Ilmu Kesehatan dan Kedokteran Vol. 4 No. 3 (2026): Juli : Termometer: Jurnal Ilmiah Ilmu Kesehatan dan Kedokteran
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/termometer.v4i3.6688

Abstract

Dengue Hemorrhagic Fever (DHF) remains a serious public health problem in Tarakan City, Indonesia, with case numbers fluctuating annually. This study aimed to analyze trends in DHF incidence and map the spatial distribution of cases and the Larval-Free Index (LFI) in Tarakan City from 2016 to 2025. A descriptive observational study with a retrospective approach was conducted using secondary data obtained from the Tarakan City Health Department. The variables analyzed included the number of DHF cases, incidence rate (IR), case fatality rate (CFR), endemicity status of subdistricts, and the 2025 LFI. Descriptive analysis and Geographic Information System (GIS)-based spatial mapping were performed. The results showed fluctuating trends in DHF incidence throughout the study period. In 2025, 90 cases were reported, with an IR of 35.2 per 100,000 population and a CFR of 1.11%. Most cases occurred in endemic areas. Spatial analysis revealed an uneven distribution of cases, with some urban villages showing lower LFI values but higher dengue incidence. These findings indicate that GIS-based mapping can support the identification of priority areas for surveillance, vector control, and sustainable larval source reduction programs.
Efektivitas E-Modul Berbasis Video Terhadap Peningkatan Keterampilan Klinis Mahasiswa Pada Pembelajaran Skill Laboratory Ni&#039;mah Hidayatul Laili; Avinda Sari Kanthi Rahayu; Eka Nurul HPS; Vincentia Ade Rizky; Maria Selviana Joni; Umi Latifah; Rachmat Saleh; Awaluddin Awaluddin; Nasrullah Tamrin; Adipura Atmadja Egok; Tri Astuti Sugiyatmi
Science: Indonesian Journal of Science Vol. 2 No. 5 (2026)
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/sciencejurnalkesehatanmasyarakatdanteknologiterapan.v2i5.403

Abstract

Pembelajaran keterampilan klinis pada mahasiswa kedokteran merupakan komponen penting dalam membentuk kompetensi profesional yang terstandar. Proses pembelajaran di skill laboratory sering menghadapi kendala, seperti keterbatasan waktu praktik, variasi kemampuan mahasiswa, serta kurangnya media pembelajaran yang dapat diakses secara fleksibel. . Pembelajaran keterampilan klinis di skill laboratory membutuhkan media yang efektif untuk menunjang peningkatan kemampuan mahasiswa. Penelitian ini bertujuan untuk menganalisis efektivitas e-modul berbasis video terhadap peningkatan keterampilan klinis mahasiswa. Metode penelitian menggunakan desain kuasi-eksperimen dengan pendekatan one group pre-test dan post-test design melalui kuesioner yang diberikan sebelum dan sesudah penggunaan e-modul pada mahasiswa. Data dianalisis secara deskriptif dengan membandingkan hasil sebelum dan setelah intervensi. Hasil menunjukkan adanya peningkatan pemahaman materi, keterampilan prosedural, serta kepercayaan diri mahasiswa dalam melakukan tindakan klinis. Sebagian besar responden juga memberikan penilaian positif terhadap kemudahan penggunaan, kejelasan materi, dan fleksibilitas akses e-modul berbasis video. Kesimpulan penelitian ini adalah e-modul berbasis video efektif dalam meningkatkan keterampilan klinis mahasiswa dan layak digunakan sebagai media pembelajaran pendukung pada kegiatan skill laboratory.
Pemanfaatan Daun dan Bunga Senduduk (Melastoma malabathricum L.) sebagai Pewarna Alami Alternatif Pengganti Eosin 2% pada Identifikasi Telur Trichuris trichiura Umi Latifah; Vincentia Ade Rizky; Maria Selviana Joni; Rachmat Saleh; Nasrullah Tamrin; Adipura Atmadja Egok; Awaluddin Awaluddin
Jurnal Ilmu Kedokteran dan Kesehatan Indonesia Vol. 6 No. 2 (2026): Juli: Jurnal Ilmu Kedokteran dan Kesehatan Indonesia
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jikki.v6i2.11216

Abstract

Examination of helminth eggs in fecal samples remains the primary method for diagnosing helminth infections. A 2% eosin stain is commonly applied to improve contrast in microscopic preparations; however, synthetic dyes have limitations due to their chemical properties and low environmental friendliness. The senduduk plant (Melastoma malabathricum L.) contains natural pigments such as anthocyanins and flavonoids, which may serve as potential natural staining agents. This study aimed to evaluate the effectiveness of senduduk leaf and flower extracts as alternative stains to replace 2% eosin in identifying Trichuris trichiura eggs. This research used a laboratory experimental design consisting of one control group and five treatment groups. The treatments included flower extract with a flower-to-distilled water ratio of (1:5), pure flower extract (100%), flower extract with 70% alcohol (1:5), leaf extract with distilled water (5:1), and leaf extract with 70% alcohol (5:1). The results showed that all extract preparations were able to stain the microscopic slides, enabling clear observation of T. trichiura egg morphology. Extracts using alcohol as a solvent produced stronger and clearer contrast compared to those prepared with distilled water. In conclusion, senduduk leaf and flower extracts demonstrate potential as environmentally friendly natural alternative stains for parasitological microscopic examinations.
Co-Authors Adipura Atmadja Egok AINA FITRIA AULIA Akmal, Dzul Anna Teresia Marbun, Romauli Asvia Rahayu Asvia Rahayu Asvia Rahayu Avinda Sari Kanthi Rahayu Awaluddin Awaluddin Awaluddin Dian Pratiwi Eka Nurul H.P.S Eka Nurul HPS HAFIKA DALIMUNTHE Hutabarat, Sintya IKKE ELMALIA PUTRI Indriani Indriani Jayanti, Neti Eka Kartini . Krisdianilo, Vinsensius Krisdianilo, Visensius Laili, Ni'mah Hidayatul Manurung, Arnita Elisabet Maria Selviana Joni Morischa Deyla Sitanggang Nasrullah Tamrin Nasution, Salmiah Neti Eka Jayanti Ni&#039;mah Hidayatul Laili Nopita Sari Manalu, Dian Rachmat Saleh Rahayu, Asvia Rahmawati Riska Wahyuni Romaulianna Theresia Marbun Sa'adah Siregar Sa'adah Siregar Saadah Siregar SAADAH SIREGAR SAADAH SIREGAR Saadah Siregar, Saadah Sa’adah Siregar Selda Lemriani Manullang Siregar, S'aadah Siregar, Sa'adah Siregar, Sa’adah Siregar, Sa’adah Sitanggang, Morischa Deyla SITI KHADIJAH Siti Ramadhani Sri Mulyani Suci Mustika Mawardani Sutriyawan, Agung Syafrina Ginting, Suventi Syukurin Juliani Gulo Tri Astuti Sugiyatmi Umi Latifah Umi Latifah Visensius Krisdianilo Visensius Krisdianilo Visensius Krisdianilo Visensius Krisdianilo, Visensius Wenda Putri Natal Saragih